Yulia Sari Ismail
Biology Department, Faculty of Mathematics and Natural Sciences, Syiah Kuala University

Published : 5 Documents
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HIV Genotype Analysis from HIV Infected Patients in East Java Area Ismail, Yulia Sari; Soetjipto, Soetjipto; Wasito, Eddy Bagus; Nasronudin, Nasronudin
Jurnal Natural Volume 12, Number 2, September 2012
Publisher : Syiah Kuala University

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Abstract

Human Immunodeficiency Virus type 1 (HIV-1) has been known to cause Acquired Immune Deficiency Syndrome (AIDS) disease and has been alaso divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with host can affect the severity of the disease. This study was aimed to analyze HIV-1 genotypes circulating in HIV/AIDS patients from the East Java region descriptively. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia.  This study used blood plasma from patients who had been tested to be HIV positive who were seeking treatment or are reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant genotypes in East Java belonged to one group of Circulating Recombinant Form (CRF), namely CRF01_AE and CRF3x_01B, which has been also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) were in the same branch with CRF01_AE and CRF3x_01B, except one sample (HIV40) was in the same branch with subtype B.
Potensi air nira aren (Arenga pinnata Merr.) sebagai sumber isolat bakteri asam asetat (BAA) Yunita, Yunita; Ismail, Yulia Sari; Maha, Feni Wahyuni
Journal of BioLeuser Vol 1, No 3 (2017): Desember 2017
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, University of Syiah Ku

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Aren (Arenga pinnata Merr.) merupakan salah satu tumbuhan palma yang dapat tumbuh dengan baik di Aceh. Salah satu produk yang dapat dihasilkan oleh aren adalah nira. Nira merupakan cairan manis yang diperoleh dari tandan bunga yang belum mekar. Kadar air pada nira segar berkisar antara 80 – 85% dan sukrosa sekitar 15%. Keadaan tersebut sangat cocok untuk pertumbuhan mikroorganisme. Isolasi dan karakterisasi jenis-jenis bakteri pada produk tumbuhan seperti nira aren sampai saat ini masih terbatas. Selain itu, mengingat Aceh merupakan salah satu daerah persebaran tumbuhan aren dan potensi nira aren untuk menghasilkan isolat Bakteri Asam Asetat (BAA), maka dilakukan penelitian yang bertujuan untuk mengidentifikasi bakteri yang berperan dalam aktivitas fermentasi asam asetat pada nira aren. Parameter yang diamati pada penelitian ini adalah morfologi koloni dan morfologi sel. Morfologi yang diamati pada penelitian ini adalah morfologi koloni dan morfologi sel. Morfologi koloni diamati dengan cara menumbuhkan isolat pada media padat PYG, kemudian bentuk, warna, tepian dan elevasi diamati secara visual. Sementara itu morfologi sel diamati dengan menggunakan pewarnaan Gram. Hasil penelitian menunjukkan bahwa terdapat empat isolat BAA yang diisolasi dari air nira aren (Arenga pinnata Merr.). Berdasarkan buku Bergey’s Determinative Bacteriology, keempat isolat tersebut tergolong ke dalam famili Acetobacteriaceae. Oleh karena itu aren (Arenga pinnata Merr.) berpotensi sebagai tumbuhan yang bisa menghasilkan isolat BAA.
Isolasi, karakterisasi dan uji aktivitas antimikroba bakteri asam laktat dari fermentasi biji kakao (Theobroma cacao L.) Ismail, Yulia Sari; Yulvizar, Cut; Putriani, Putriani
Journal of BioLeuser Vol 1, No 2 (2017): Agustus 2017
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, University of Syiah Ku

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Bakteri Asam Laktat (BAL) merupakan kelompok bakteri yang mampu mengubah karbohidrat  (glukosa) menjadi asam laktat. Penelitian ini bertujuan untuk mengisolasi dan mengetahui karakteristik BAL yang berperan dalam aktivitas fermentasi asam laktat pada biji kakao (Theobroma cacao L.).  Parameter yang diamati adalah morfologi koloni (bentuk, tepian, elevasi dan warna isolat), morfologi sel (bentuk sel dan pewarnaan Gram), uji biokimia (TSIA, indol, motilitas, MR-VP, tipe fermentasi, ketahanan suhu 15oC dan 37oC, ketahanan garam 5%, 6.5% dan 10%, serta katalase,) dan uji aktivitas antimikroba.  Dari hasil penelitian ini diperoleh tiga isolat BAL yang merupakan kelompok bakteri Gram positif berbentuk basil dan kokus. Ketiga isolat secara umum memiliki morfologi koloni yang berbeda yaitu berbentuk bundar, tepian licin, elevasi cembung dan datar, berwarna krem dan putih susu. Hasil uji biokimia menunjukkan bahwa ketiga isolat termasuk famili Lactobacillaceae, genus Lactobacillus (isolat FBK 1 dan FBK 3) dan genus Enterococcus (isolat FBK2).  Pada uji daya hambat (aktivitas antimikroba), ketiga isolat mampu menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus.
Karakterisasi Bakteri Asam Laktat Indegenous Dari Jruek Drien, Provinsi Aceh Yulvizar, Cut; Ismail, Yulia Sari; Moulana, Ryan
Jurnal Teknologi dan Industri Pertanian Indonesia Vol 7, No 1 (2015): Vol.(7) No.1, April 2015
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (645.833 KB) | DOI: 10.17969/jtipi.v7i1.2831

Abstract

(Characterization Of Lactit Acid Bacteria Indegenous From Jruek Drien, Aceh ProvinceABSTRACT. Jruek drien is traditional fermented Durian. The purpose of this research was to obtain Lactic acid bacteria indigenous from jruek drien for development potential probiotic. The research was conducted at Laboratory of Microbiology, Department of Biology, Faculty of Mathematic and Natural Science of Syiah Kuala University from Februari – Juny 2015. Sample was taken from Aceh Jaya Regency, Aceh Barat Regency, Aceh Barat Daya Regency, Nagan Raya Regency dan Aceh Selatan Regency. The results showed that there are 9 Lactic acid bacteria indigenous isolate from jruek drien.
The Potential Endophytic Bacteria Isolated from Rice (Oryza sativa) as Biofertilizer Putriani, Putriani; Fitri, Lenni; Ismail, Yulia Sari
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.252 KB) | DOI: 10.15294/biosaintifika.v11i2.18401

Abstract

Endophytic bacteria are beneficial microorganisms that interact with host plants without causing any interference or damage to plants. This research aimed to obtain endophytic bacteria isolated from the root tissue of rice plants (Oryza sativa L.) which have potential to produce IAA hormones,, identify the endophytic bacteria in morphologically and physiologically, and analysis of the selected isolate 16S rRNA genes. Based on the results of this research, there  were six endophytic bacteria isolates obtained. They have high morphological diversity and differen ability producing IAA hormones. The highest concentration of IAA (425 ppm) was obtained  isolates from  EAP3. Isolate EAP3 also produce inhibit the growth of Xanthomonas oryzae with a 5.2 mm inhibition zone. Based on the biochemical test, EAP3 had 60% similarity with Enterobacter asburiae. Analysis of the 16S rRNA gene showed that EAP3 had the highest similarity with Enterobacter asburiae strain U4 by 99%. This research data is considered as new information about the potential of endophytic bacteria from the roots of rice plants (Oryza sativa L.) which is capable of producing IAA hormones and is able to inhibit pathogenic bacteria. This research provides information that can be used as a basis for developing endophytic bacteria as biological fertilizers.