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Ekstraksi Isoflavon Kedelai dan Penentuan Kadarnya Secara Ultra Fast Liquid Chromatography (UFLC) Sartini, .; Djide, M. Natsir; Permana, A. Dian; Ismail, .
SAINSMAT Vol 3, No 2 (2014): September
Publisher : SAINSMAT

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Abstract

Isoflavon merupakan komponen polifenol utama dalam kacang kedelai. Isoflavon kedelai utamanya dalam bentuk glikosida (Genistin dan Daidzin) dibanding bentuk aglikonnya (Genistein dan Daidzein). Tujuan penelitian ini adalah untuk mengetahui pengaruh metode ekstraksi terhadap kandungan isoflavonnya (Genistein, Daidzein, Genistin, Daidzin). Isoflavon kedelai diekstraksi dengan beberapa cara, yaitu: 1) kacang kedelai tanpa kulit ari diblender dengan air panas, 2). Kacang kedelai tanpa kulit ari diblender dengan etanol 70 % , 3) kacang kedelai utuh diekstraksi secara maserasi dengan aseton 70 %, dan 4) kacang kedelai utuh diekstraksi secara maserasi dengan metanol.. Perbandingan kacang kedelai dan cairan penyari 1:10. Ekstrak yang diperoleh dianalisis kadar isoflavonnya menggunakan kromatografi cair kecepatan tinggi. Hasil penelitian menunjukkan total isoflavon tertinggi (dihitung sebagai total genistein, daidzein, genistin, daidzin) ada pada kacang kedelai utuh yang diekstraksi dengan metanol.Kata Kunci : Metode Ekstraksi, Isoflavon, Kedelai (Glycine max L.), Ultra Fast Liquid Chromatography
PERBANDINGAN AKTIVITAS ANTELMINTIK ALBENDAZOLE DAN LEVAMISOLE TERHADAP ASCARIDIA GALLI SECARA IN VITRO Balqis, Ummu; Hambal, Muhammad; Darmawi, .; Harris, Abdul; Rasmaidar, .; Athaillah, Farida; Muttaqien, .; Azhar, .; Ismail, .; Daud, Razali
Acta VETERINARIA Indonesiana Vol. 4 No. 2 (2016): Juli 2016
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.142 KB) | DOI: 10.29244/avi.4.2.97-102

Abstract

Penelitian ini meneliti aktivitas antelmintik albendazole dan levamisole terhadap hambatan motilitas, percepatan waktu paralisis, dan motilitas cacing Ascaridia galli dewasa secara in vitro. Sebanyak empat ekor cacing masing dibuat triplikat dalam NaCl 0,9% masing-masing dengan konsentrasi 15 mg/ml Albendazole, dan 0.6 mg/ml Levamisole. Motilitas cacing diamati pada interval 10, 20, 30, dan 40 jam. Paralisis dan kematian diamati pada tampilan tidak ada pergerakan badan pada bagian kepala dan ekor cacing. Hasil menunjukkan bahwa aktivitas albendazole dan levamisole terhadap mortalitas A. galli berturut-turut terjadi pada 40 dan 30 jam pasca inkubasi. Levamisole dapat menghambat motilitas A. galli pada jam ke 10 dan juga menyebabkan lebih awal paralisis pada 6,75 ± 0,50 jam pasca inkubasi. Kajian tersebut mengindikasikan bahwa aktivitas antelmintik levamisole lebih awal dibandingkan efek albendazole pada cacing A. galli.
NOTES ON SOME BIOLOGICAL ASPECTS OF ARCTORNIS RIGUATA SNELLEN (LEPIDOPTERA: LYMANTRIIDAE) SUTRISNO, HARI; SUPUTA, .; PURNOMO, HARI; POLANDONO, SISWO; WALUYO, CIPTO; UBAIDILLAH, ROSICHON; DARMAWAN, .; ISMAIL, .; HIDAYAT, IMAN; WIDYASTUTI, NUNUK
HAYATI Journal of Biosciences Vol. 20 No. 1 (2013): March 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (40.309 KB) | DOI: 10.4308/hjb.20.1.46

Abstract

Arctornis riguata Snellen is one of lymantriids which attacked a vast range of mango trees in Probolinggo at the beginning of 2011. About 1.2% mango trees from nine sub-districts have been defoliated by the larvae of this species. The larvae of this genus have been reported to forage at Anacardiacea as well; however, they have never been reported to forage at cultivated mango trees in Indonesia. Since there is no biological information of this species, thus, a study on some biological aspects of this species is needed. This study was conducted in the field as well as in the laboratory during 4 months (March-July 2011). The diagnostic characters of this species are black scale at dorsal antenna on both male and female and slightly setae particularly at the costal angle of valve on the male genitalia. Life span of this species is in the range of 30-37 days. This study also found four natural enemies of A. riguata i.e.: Bleparipa sp. (Diptera: Tachinidae), Euagathis sp. (Hymenoptera: Brachonidae), Theronia sp. (Hymenoptera: Ichneumonidae), and Brachymeria lasus (Hymenoptera: Chalcididae). Moreover, a single fungal pathogen of this species also was identified, i.e. Isaria fumosorosea Wize. Having these results, we considered that to control A. riguata, one need to conserve the native natural enemies by manipulating their environment.  
The Shifting of Burden of Proof on Corruption Offences in Indonesia After The Ratification of United Nations Convention Against Corruption (UNCAC) 2003 Mulyadi, Lilik; Ismail, .
The Southeast Asia Law Journal Vol 2, No 1 (2016)
Publisher : Postgraduate of Jayabaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.527 KB) | DOI: 10.31479/salj.v2i1.77

Abstract

This article describes the result  of a research  regarding  the shifting of burden  of proof  on corrup-  tion offenses in the Indonesia after the ratification of United Nations Convention Against Corruption (UNCAC) 2003. T he article uses normative research which regulation, conceptual, case and compar- ative approach. Such research emphasizes interpretation and legal construction to obtain some legal norms, conception, regulation list and its implementation in concerto cases. Regulation  and concep-  tual approach to used how to know, existences, consistency and harmonization regarding the shifting of burden of proof upon corruption offenses in legislation body. The cases approach uses comparative law regarding the reversal burden of proof upon corruption offender between Indonesia and the other countries. This research shows that the shifting of burden of proof has never yet applied for in the corruption cases Indonesia. The Indonesian corruption regulation policy, especially  article 12B, 37, 37A, 38B apparently it’s not cleaq and disharmony to norm of sudden charge of fortune the shifting of burden of proof formulation in connection with United Nations Convention Against Corruption 2003 (KAK 2003). So, necessary (needs) of modification sudden charge of fortune shifting of burden of proof formulation which preventive, represive and restorative characteristic.Keywords: Corruption; The Shifting of Burden of Proof; Balanced Probability of Principles         
MP-12 EFFECT OF AKBISPROB SUPPLEMENTATION ON ANTIBACTERIAL-PRODUCING LACTIC ACID BACTERIA (LAB) ISOLATED FROM LAYING HENS INTESTINE Nurliana, .; Coumandary, Sema; Farimansysh, Fashihah Rahmah Noya; Sugito, .; Masyitha, Dian; Darmawi, .; Erina, .; Gholib, .; Rahmi, Erdiansya; Ismail, .
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Generally, Bacteria in the intestine consist of beneficial bacteria and bacteria that have the potential to disrupt animal health such as pathogenic bacteria. The presence of pathogenic bacteria is one of the factors that cause gastrointestinal dysfunction, but can be suppressed by maintaining the presence of beneficial bacteria for the digestive tract, especially the type of Lactic Acid Bacteria (LAB) (Astuti, 2016). LAB has antagonistic activity because it is able to inhibit pathogenic bacteria so it can compete to maintain normal flora balance in the digestive tract.  The main inhibitory activity of LAB is caused by the accumulation of primary metabolites such as lactic acid, acetic acid, ethanol, and carbon dioxide. In addition, LAB is also capable of producing antibacterial compounds such as bacteriocins (Furtado et al., 2014). Naturally LAB has existed in the digestive tract of chickens, but it is easy to experience changes in the amount due to the influence of given the feed (Widodo et al., 2015). Feed and the environment can affect the composition of microbes in the digestive tract in chickens(Ghadban, 2002; Apajalahti et al., 2004). Provision of fermented feed can improve the composition of intestinal microflora and increase the number of LAB. Fermented feed is generally easily biodegradable and has a higher nutritional value than the original ingredient and it can reduce the pH of the digestive tract.AKBISprob is an alternative product to increase poultry production in the form of supplement made from a mixture of soybean wate, and palm kernel meal which is fermented with Aspergillus niger.  The fermentation process using Aspergillus niger can reduce the crude fiber contained in AKBIS, because the mold can produce amylase, pectinase, amyloglucosidase and cellulase enzymes which can degrade cellulose so that it is easier to digest. In addition, Aspergillus niger also produces metabolites in the form of citric acid which is a component that can reduce intestinal pH so that it is suitable for LAB growth. Based on research conducted by Nurliana et al. (2016; 2017), supplementation of 4% AKBISprob can maintain the chickens production and health as well increase the number of LAB and reduce the number of pathogenic bacteria such as Escherichia coli and Salmonella sp. in chickens intestine. Thus creating a balance of microflora in the digestive tract of laying hens. Based on the above reasons, it is necessary to conduct research on antibacterial detection of BAL in laying hens that have been given AKBISprob.