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THE INFLUENCES OF CHITOSAN FROM Penaeus monodon ON C-REACTIVE PROTEIN EXPRESSION IN AORTA AND CORONARY ARTERY OF SPRAGUE DAWLEY RATS BY HIGH FAT INDUCTION

JOURNAL OF COASTAL DEVELOPMENT Vol 16, No 1 (2012): Volume 16, Number 1, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

The objective of this study was to analyze the influences of chitosan on C-Reactive Protein expression in aorta and coronary artery of Sprague Dawley rats by high fat induction. The animals for this study were 20 adult male rats divided into four groups, i.e. group I as the control was fed with basal diet containing normal fat for 3 months, group II was fed diet containing high fat for 3 months, group III was fed diet containing high fat and given chitosan 180 mg per kg body weight per day orally in  2 ml aquadest for 3 months, group IV was fed diet containing high fat for 3 months and  after 1 month given chitosan 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the hearts were collected to histopathological and immunohistochemical analysis by immunohistochemistry streptavidin-biotin method. C-Reactive Protein expression in aorta was negative. Chitosan was able to prevent atheroma plaque formation in coronary artery and CRP may involve in atherosclerosis.

MIKROANATOMI HEPAR MENCIT (Mus musculus) SETELAH PEMBERIAN KITIN PER-ORAL

JURNAL SAINS DAN MATEMATIKA Volume 17 Issue 2 Year 2009
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

ABSTRACT--The  objective of  the experiment  was determines  liver  microanatomy  and diet consumption  n  the Mus muscalus  after give of  chitin. This research  used  completely  randomized  design. There  were  fow  concentration  of chitin, 0 mg chitin per day; 1,3 mg chitin per day; 1,95  mg chitin per day; 2,6 mg chitin per day. Anova  was  used  in data  analysis  and  LSDT test  in 57o  level. The  result  of experiment indicated  that  the  chitin was  not cause alteration  on  liver microanatomy.Keywords:  chittin, Mus musculus, liver microanatomy

Chitosan Mempertipis Dinding dan Memperbesar Diameter Lumen Arteri Koroner Tikus Putih yang Diberi Pakan Lemak Tinggi (CHITOSAN COULD THINEN WALL AND WIDEN LUMEN DIAMETER CORONARY ARTERY OF SPRAGUE DAWLEY RATS INDUCED BY HIGH FAT RATION)

Jurnal Veteriner Vol 14, No 3 (2013)
Publisher : Jurnal Veteriner

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Abstract

The objective of this study was to analyze the influence of chitosan on coronary artery wall thicknessand lumen diameter of Sprague Dawley rats induced by high fat ration. The animals for this study weretwenty adult male rats divided into four groups, i.e. group I as the control was fed with basal rationcontaining normal fat for 3 months, group II was fed ration containing high fat for three months, group IIIwas fed ration containing high fat and given chitosan 180 mg per kg body weight per day orally in 2 mLaquadest for 3 months, group IV was fed ration containing high fat for three months and after one monthgiven chitosan 180 mg per kg body weight per day orally in 2 mL aquadest for two months. Each groupconsisted of five animals. After 90 days, the rats were necropsied and the hearts were collected tohistopathological. The difference between treatments was statistical analysis by Anava, and continued byLowest Significant Difference Test to locate the difference. Normal fat ration did not influence coronaryartery wall thickness and coronary artery lumen diameter in rats. High fat ration increased coronaryartery wall thickness and chitosan could be decreased coronary artery wall thickness in rats. High fatration + chitosan that given simultaneously could be increased coronary artery lumen diameter in rats.Based on the result of this study, it was concluded that high fat ration was a major factor able to causeatherosclerosis and chitosan was able to prevent atheroma plaque formation.

Profile Triglycerides Japanese Quail (Coturnix coturnix japonica) After Giving Turmeric (Curcuma longa) Powder

International Journal of Science and Engineering Vol 8, No 1 (2015)
Publisher : Chemical Engineering Diponegoro University

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Abstract

The purpose of this study was to determine the triglyceride profile of Japanese quail (Coturnix coturnix japonica) after being given the parameters of turmeric (Curcuma longa) powder; feed intake, profiles of liver triglycerides, profiles of serum triglycerides and profiles of meat triglycerides. This study uses an experimental method with A Completely Randomized Design Pattern. Test animals used were 45 female Japanese quails were divided into 3 groups, namely; P0: quail were not given turmeric powder, P1: quail were given turmeric powder a dose of 54 mg/quail/day, P3: quail were given turmeric powder a dose of 108 mg/quail/day. Each group with 5 replications. Each repeat consists of 3 Japanese quails. Provision of treatment every day for 30 days starting from the age of 14 days. Data were analyzed using analysis of variance (ANOVA) followed by Duncans test with 95% confidence interval (α = 0.05 level). Analysis of the data used is software Minitab software 16. Results showed that the levels of turmeric powder are given in Japanese quail significant (P˂0.05) on liver triglyceride levels, serum and Japanese quail meat but had no significant effect (P˃0.05) on consumption feed. The results showed that the optimal dose of turmeric powder to lower triglyceride levels of Japanese quail is 108 mg/quail/day as evidenced by the highest decrease in liver triglyceride profiles, profiles of serum triglycerides and triglyceride profiles of Japanese quail meat compared with other treatments.

EFEK CHITOSAN PADA HISTOPATOLOGIS AORTA TIKUS PUTIH YANG DIBERI PAKAN LEMAK TINGGI

JURNAL ANATOMI FISIOLOGI Vol 23, No 1 (2015): VOLUME XXIII, NOMOR 1, MARET 2015
Publisher : JURNAL ANATOMI FISIOLOGI

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Abstract

The objective of this study was to analyze the influence of chitosan on aorta histopatologic of Sprague Dawley rats induced by high fat ration. The animals for this study were twenty adult male rats divided into four groups, i.e. group I as the control was fed with basal ration containing normal fat for 3 months, group II was fed ration containing high fat for 3 months, group III was fed ration containing high fat and given chitosan 180 mg per kg body weight per day orally in 2 ml aquadest for 3 months, group IV was fed ration containing high fat for 3 months and after 1 month given chitosan 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the aortas of heart were collected to histopathological. Histopathologic analysis of aortas using hematoxylin-eosin staining method and were analysis by descriptive. The rats given normal diet did not induce atheroma plaque. The rats given high fat diet induced atheroma plaque. The rats given high fat and given chitosan simultaneously did not induce atheroma plaque. The rats given high fat and after 1 month given chitosan 40% did not induce atheroma plaque and 60% induced atheroma plaque. Based on the result of this study, it was concluded that high fat ration was a major factor able to cause atherosclerosis and chitosan was able to prevent atheroma plaque formation.   Key words: Atherosclerosis, aorta, atheroma plaque, chitosan, high fat ration.

Potensi Cangkang Udang Laut (Penaeus monodon F.) Terhadap Profil Lipid Tikus Putih Hiperlipidemia

Bioma Vol. 17, No.1, Tahun 2015
Publisher : Bioma

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Abstract

The objective of this study was to analyze the potency of sea shrimp carapace (Penaeus monodon F.) on lipid profiles of Sprague Dawley rats induced by high fat ration. The animals for this study were twenty adult male rats divided into four groups, i.e. group I as the control was fed with basal ration containing normal fat for 3 months, group II was fed ration containing high fat for 3 months, group III was fed ration containing high fat and given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 3 months, group IV was fed ration containing high fat for 3 months and after 1 month given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the blood were collected to analyzes lipid profiles. Lipid profiles were measured using colorimetric enzymatic method and Roche/Hitachi cobas c systems automatically calculate.  The difference between treatments was statistical analysis by Anava, and continued by Lowest Significant Difference Test to locate the difference. The result showed that high fat diet decreased HDL level, but increased total cholesterol level and LDL level. Sea shrimp carapace increased HDL but decreased total cholesterol level and LDL level in rats by high fat induction.   Keywords: Sea shrimp carapace, Sprague Dawley rats, lipid profiles

Ratio Kadar Ldl/Hdl Tikus Putih Sprague Dawley Hiperlipidemia Setelah Diberi Cangkang Udang Laut (Penaeus monodon F.)

Bioma Vol. 17, No.2, Tahun 2015
Publisher : Bioma

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Abstract

The objective of this study was to analyze the ratio of LDL/HDL level of Sprague Dawley rats induced by high fat ration after given of sea shrimp carapace (Penaeus monodon F.). The animals for this study were twenty adult male rats divided into four groups, i.e. group I as the control was fed with basal ration containing normal fat for 3 months, group II was fed ration containing high fat for 3 months, group III was fed ration containing high fat and given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 3 months, group IV was fed ration containing high fat for 3 months and after 1 month given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the blood were collected to analyzes ratio of LDL/HDL level. LDL level and HDL level were measured using colorimetric enzymatic method and Roche/Hitachi cobas c systems automatically calculate.  The difference between treatments was statistical analysis by Anava, and continued by Lowest Significant Difference Test to locate the difference. The result showed that high fat diet increased ratio of LDL/HDL level and Sea shrimp carapace decreased ratio of LDL/HDL level.   Keywords: Sea shrimp carapace, Sprague Dawley rats, ratio of LDL/HDL level

PENGARUH PEMBERIAN LARUTAN KOMBINASI MIKROMINERAL (Cu, Fe, Zn, Co), VITAMIN (A, B1, B12, C) DAN JUS BUAH MENGKUDU (Morinda citrifolia L.) SEBAGAI AIR MINUM TERHADAP KONSUMSI MINUM DAN BOBOT KARKAS PUYUH (Coturnix coturnix japonica L.).

JURNAL ANATOMI FISIOLOGI Vol 23, No 2 (2015): Volume XXIII, Nomor 2, Oktober 2015
Publisher : JURNAL ANATOMI FISIOLOGI

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Abstract

Quail (Coturnix-coturnix japonica L.) was becoming more popular in Indonesia because of the product that produce was substantial enough like the meat and egg. This animal often used as an experiment in order to enhance it’s productivity. This research was to find out the potential of giving combination of micromineral (Cu, Fe, Zn, Co), vitamin (A, B1, B12, C) and morinda juice (Morinda citrifolia L.) solution as drinking water to drink consumption and weight of carcass. The treatment was begun when the quail was in age of 4 to 16 weeks. The experiment in this research was nonfactorial experimentation with complete random plan (RAL). The obtained data was analyzed by using the analysis of varian (ANOVA) and if there was a real difference then it will be done an advanced testing by using the Duncan test in confidence level 95%. According to the analysis result towards the drink consumption and the weight of carcass indicate any real difference inter cluster of quail whose it’s drink was added by combination of micromineral (Cu, Fe, Zn, Co), vitamin (A, B1, B12, C) and morinda juice with control, in conclusion that giving combination of micromineral (Cu, Fe, Zn, Co), vitamin (A, B1, B12, C) and morinda juice has the potency in the drinking management. Keywords : Coturnix coturnix japonica L., micromineral, vitamin, morinda juice

PENGARUH PEMBERIAN MIKROMINERAL (Fe, Co, Cu, Zn), VITAMIN (A, B1, B12, C) DAN JUS MENGKUDU (Morinda citrifolia L.) TERHADAP KONSUMSI PAKAN, BOBOT LEMAK ABDOMINAL DAN JUMLAH FOLIKEL OVARIUM YANG BERKEMBANG PADA PUYUH (Coturnix coturnix japonica L.)

JURNAL ANATOMI FISIOLOGI Vol 23, No 2 (2015): Volume XXIII, Nomor 2, Oktober 2015
Publisher : JURNAL ANATOMI FISIOLOGI

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Abstract

The purpose of the research was evaluated feed consumption, abdominal fat and amount mature folliclesfrom cultivated with combination solution of microelements (Fe, Co, Cu, Zn), vitamins (A, B1, B12, C) and morinda juice (Morinda citrifolia L.) as drinking water to optimization the productincultivation of the quail.  Sixty quails (Coturnix coturnix japonica L.) fourteen days were divided into four treatments of combination solution microelements, vitamins and morinda juice with 15 quails in each treatments. The treatments were control, microelements and vitamins, morinda juice, and minroelements, vitamins and morinda juice. Experimental data were analyzed by using ANOVA based on completely randomized design. The result showed that combination solution of microelements, vitamins and morinda juice significant in feed consumption. Morinda juice increased feed consumption so that solution must not be used in cultivation technique of quail. Microelements and vitamins combination decreased feed consumption but not influential to mature follicles. Keywords: microelements, vitamins, morinda juice, Coturnix coturnix japonica L.

KADAR APOPROTEIN A DAN APOPROTEIN B SERUM DARAH TIKUS PUTIH Sprague Dawley HIPERLIPIDEMIA SETELAH DIBERI CANGKANG UDANG LAUT (Penaeus monodon F.)

JURNAL ANATOMI FISIOLOGI Vol 23, No 2 (2015): Volume XXIII, Nomor 2, Oktober 2015
Publisher : JURNAL ANATOMI FISIOLOGI

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Abstract

  The objective of this study was to analyze the  apoprotein  A  and apoprotein B level of Sprague Dawley rats induced by high fat ration after given of sea shrimp carapace (Penaeus monodon F.). The animals for this study were twenty adult male rats divided into four groups, i.e. group I as the control was fed with basal ration containing normal fat for 3 months, group II was fed ration containing high fat for 3 months, group III was fed ration containing high fat and given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 3 months, group IV was fed ration containing high fat for 3 months and after 1 month given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the blood were collected to analyzes  of apoprotein A and apoprotein B level. Apoprotein A level and Apoprotein B level were measured using colorimetric enzymatic method and Roche/Hitachi cobas c systems automatically calculate.   The result showed that high fat diet increased  of apoprotein B level, sea shrimp carapace increased  of apoprotein A level and decreased  of apoprotein B level.   Keywords : Sea shrimp carapace, Sprague Dawley rats, apoprotein A and apoprotein B level