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In Vitro Bioremediation of Toluene Contaminated Soil With Addition of Local Strains Bacillus Irianto, Agus; Komar, M. Syamsul
Jurnal Mikrobiologi Indonesia Vol 5, No 2 (2000): JURNAL MIKROBIOLOGI INDONESIA
Publisher : Jurnal Mikrobiologi Indonesia

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Abstract

Bioremediatlon is the use of microorganism to remove or detoxify toxic or unwanted chemicals in an environment. Among those chemicals are hydrocarbon such as toluene. Environment pollution by substances such as toluenewill destroy the environment and resulting reduction of the biodiversity of organisms. Some species of microorgan.isms which capable to adapt and degrading toxic substance will exist. However, to optimise bioremediation nutrientaddition such as N and introducing degrader microbes are necessary. This study tried to solved the problem by ureaaddition in 0.25% w/v concentration and introducing promising local bacilli isolates namely UK41 and UX44 respec.tively as well as their mixture. The parameters measured was toluene, COD, BOD, pH, microbial number, DO andCO2. The best result was revealed from introduction of mixture of U41 and U44. It means that local isolates of bacilliU41 and U44 are prospective to be used as soil bioremedlation agent for hydrocarbon contaminated soil.
BIODEGRADASI PETROLEUM MENGGUNAKAN BAKTERI INDIGENOUS DARI PERAIRAN MUARA SUNGAI DONAN CILACAP Risdiyanto, Risdiyanto; Irianto, Agus; Sastranegara, Moh. Husein
Buletin Limbah Vol 16, No 3 (2013): Nopember 2013
Publisher : Buletin Limbah

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Abstract

BIODEGRADASI PETROLEUM MENGGUNAKAN BAKTERI INDIGENOUS DARI PERAIRAN MUARA SUNGAI DONAN CILACAP.Tumpahan petroleum di perairan menjadi masalah karena dapat menimbulkan kontaminasi dan berpengaruh terhadap makhluk hidup dan manusia karena komponen petroleum bersifat toksik dan karsinogenik. Teknik remediasi secara biologi (bioremediasi) menggunakan mikroorganisme terutama bakteri indigenous yang menjadi solusi dalam pengendalian kontaminasi petroleum. Efektivitas bioremediasi petroleum di lingkungan menggunakan bakteri indigenous sangat dipengaruhi oleh faktor-faktor lingkungan dan jumlah bakteri. Penggunaan bakteri indigenous dalam aplikasi bioremediasi akan lebih efektif karena bakteri sudah teradaptasi dengan baik pada lingkungan dan mampu berkembang baik. Tujuan penelitian ini adalah untuk mengetahui ragam isolat bakteri indigenous yang dapat ditemukan pada sedimen terkontaminasi petroleum di kawasan perairan muara Sungai Donan Cilacap, efektivitas konsorsia bakteri indigenous dalam mendegradasi petroleum, dan persepsi masyarakat tentang pencemaran petroleum. Penelitian ini menggunakan metode survei dan eksperimental. Hasil
The Effect of Length of A3-51 Probiotic Supplementation to The Number of Intestine Enterobacteriaceae in Oreochromis niloticus Desnilasari, Dewi; Sukanto, Sukanto; Irianto, Agus
SAINTEK PERIKANAN Vol 2, No 1 (2006): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

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Abstract

The aim of this research was to know the effect of length of A3-51 probiotic  supplementation  to the number   of   intestine   Enterobacteriaceae.   Research   used   experimental   method   using   Completely Randomized  Design  which  was  based  on  4   treatments  i.e.  La  (without  probiotic),  Ll  (probiotic supplementation for 7 days), L2 (probiotic supplementation for 14 days), L3 (probiotic supplementation for 21 days). Each  treatment was repeated 3 times. The result showed that the length period of A3-51 probiotic   supplementation  very   significantly  (P<0,01)  increased  the  intestine   Enterobacteriaceae number. However, in the same time the amount of pathogenic Enterobacteriaceae decreased. In addition, probiotic supplementation improved protein, fat, and carbohydrate digestions.   Key words: Enterobacteriaceae, intestine, probiotic, nutrient digestibility
Immunologic Quality Improvement of Racing Pigeon Squabs through Induction of Cropmilk using Probiotic Lactobacillus sp. Irianto, Agus; Budiyanto, B Heru
ANIMAL PRODUCTION Vol 6, No 2 (2004): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Probiotic is live microbial cells supplementation that has beneficial effect to the host. Feeding adult pigeons (Columba livia) with food supplemented with probiotic, Lactobacillus sp., at concentration 106,108, and 1010 cells g-1 respectively have been done in order to improve the immunologic quality of the squabs. Immunologic response namely the number and activity of kidney’s macrophage were examined. Also, the pathologic signs which developed following artificial infection by intramuscular injection with Escherichia coli suspension was examined. The result indicated that adult pigeon feed with food containing probiotic have beneficial effect to the squabs (through cropmilk) by improving their immune response namely increasing the number of kidney’s macrophage and their in vitro phagocytic activity. Also, it reduced the E. coli population in the squabs’ gut. (Animal Production 6(2): 11-124 (2004) Key Words: pigeon squab, probiotic, Lactobacillus sp., immune response
Karakteristik Morfologi Mycobacterium tuberculosis yang Terpapar Obat Anti TB Isoniazid (INH) secara Morfologi Widodo, Widodo; Irianto, Agus; Pramono, Hendro
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 3 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

INH mempunyai peranan dalam menghambat sintesis asam mikolat yang merupakan komponen pembentuk dinding sel M. tuberculosis. Mengetahui pengaruh pemaparan obat INH terhadap karakteristik M. tuberculosis secara morfologi sel. Metode penelitian eksperimen dengan menggunakan Rancangan percobaan acak Lengkap dengan variasi waktu pemaparan 24 jam, 48 jam, 72 jam dan 96 jam pada konsentrasi INH 0,5 μg/ml dengan jumlah ulangan 6. Hasil penelitian menunjukkan ada pengaruh pada masing-masing perlakuan INH 0,5 μg/ml terhadap karakteristik M. tuberculosis, pada pemaparan 24 jam diperoleh persentase pertumbuhan 18,25%,  ukuran rerata sel 1,64 x 0,35 µm, persentase kerusakan sel 28,1%. Pada pemaparan 48 jam diperoleh persentase pertumbuhan sel  7,9%,  ukuran rerata sel 1,64 x 0,36 µm, persentase kerusakan sel 41%, pada pemaparan 72 jam diperoleh persentase pertumbuhan sel 7,0%,  ukuran rerata sel 1,64 x 0,32 µm, persentase kerusakan sel 53,4%, pada pemaparan 96 jam diperoleh persentase pertumbuhan sel 1,6%, ukuran rerata sel 1,61 x 0,29 µm,  persentase kerusakan sel 57%. Perubahan morfologi sel yang terpapar INH 0,5 μg/ml pada permukaan sel terjadi kerutan dan penyusutan panjang sel setelah regenerasi, permukaan sel mulai kasar dan sedikit ada kerusakan, sel mengalami lisis dan sebagian sel berbentuk oval , sel mengalami perubahan bentuk menjadi kokus. karakteristik M. tuberculosis yang terpapar INH 0,5 µg/ml mengalami perubahan morfologi sel, menjadi lebih pendek dan perubahan bentuk dari batang menjadi kokus, namun perubahan morfologi ini tidak terjadi secara permanen pada generasi berikutnya.Kata kunci       : M. tuberculosis, INH, Morfologi, 
Pengaruh Suplementasi Probiotik A3-51 terhadap Derajat Imunitas Oreochromis niloticus Didasarkan pada Angka Kuman pada Ginjal Setelah Uji Tantang dengan Aeromonas hydrophila dan Aeromonas salmonicida achromogenes Irianto, Agus; Hernayanti, Hernayanti; Iriyanti, Ning
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 2 (2006)
Publisher : Universitas Gadjah Mada

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Abstract

Objectives of this research was to know the effect of probiotic A3-51 supplementation on food to the total number of bacteria in kidney and mortality of Oreochromis niloticus after challenge test with Aeromonas hydrophila and Aeromonas salmonicida achromogenes. This research was consisted of two experiment, the first was to know the highest immunity level of fish based on the number of macrophage and its phagocytic activity. Meanwhile, the second was to know the immunity level of fish challenged with A. hydrophila and A. salmonicida achromogenes by intra-peritoneal injection. The experiment used Completely Randomized Design in triplicates. The result from the first experiment showed that the highest non spesific immune system response, by means number of macrophage, was found 21 days treatment. The second experiment showed that the highest total number of bacteria in kidney and mortality level were 20.23 x 108 cells/g and 46.67%, respectively, both was found in control fish injected intra-peritoneally with A. salmonicida achromogenes.
Efektivitas Layanan Bimbingan Kelompok untuk Meningkatkan Motivasi Belajar dan Mengurangi Perilaku Membolos Siswa Safitri, Nur’aini; Neviyarni, Neviyarni; Irianto, Agus
Konselor Vol 3, No 4 (2014): KONSELOR
Publisher : Universitas Negeri Padang

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Abstract

The low learning motivation and behavior of truant one of the problems in education that requires actions to sustainable reduction in the future. Group guidance services by leveraging dynamics group of students will obtain variety information that can help to overcome the various problems that life can be effective (KES) back. This research is a quantitative experiment design using form of quasi-experiment with the design of the non equivalent control group. The purpose of this study to increase the learning motivation and reduce behavior of truant students by implementing group guidance services. The subjects were students of grade IX from two schools, namely SMP Negeri 26 Padang (control group) and SMP Negeri 29 Padang (experimental group). Samples were taken by using purposive sampling with criteria that students who have low learning motivation and behaving truant more than three days in a month. The data collect using learning motivation scale with Likert model and student attendance recapitulation which then analyzed using the Wilcoxon Signed Ranks Test and Mann-Whitney 2 Independent Samples. The  study  revealed  that: (1) there was a significant differences between the learning motivation and behavior of truant students experimental group before and after group guidance services, where the average score of students motivation to increase and frequency behavior of truant s students decreased after treatment, (2) there was a significant differences between the learning motivation and behavior of truant students control group before and after information services, where the average score of students motivation to increase and frequency behavior of truant students decreased after treatment, (3) there  was  not  a significant difference between the learning motivation and behavior of ditching the control group students with student the experimental group after a given treatment, and (4) the application group guidance service is more effective to improve learning motivation and reduce the frequency behavior of truant students compared with information services, where the average score of motivation to learn the experimental group is higher and the frequency behavior of truant the experimental group was lower than with the control group after treatment.
Efektivitas Layanan Informasi dengan Pendekatan Contextual Teaching And Learning dalam Meningkatkan Arah Perencanaan Karier Siswa SMK Putri, Ramtia Darma; S, Neviyarni; Irianto, Agus
Konselor Vol 4, No 3 (2015): KONSELOR
Publisher : Universitas Negeri Padang

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Abstract

Direction of career planning become very important because it can guidelines students in decisions making of his career in the future.  Direction of career planning should be prepared and improved considering that not all students have a mature direction of career planning. Central Statistics Agency (BPS) of West Sumatera in August 2014 recorded the open unemployment of about 6.99%  or reach 150 thousand people are more dominated by graduates SMK as much as 11.15%. In addition, the results of AUM umum in one of the SMK was indicated problems in careers and jobs as much to 33.56%. This demonstrates that students do not have a clear understanding about direction of career planning that will be chosen. One of the efforts to improve the students direction of career planning is the information service.  This research was intended to find out the effectiveness of information service with contextual teaching and learning approach to improve the direction of career planning student of SMK.This study uses quantitative methods. This type of research is Quasi Experiment with Nonrandomized Control Group Pretest-posttest Design. The subjects were students of SMK Padang Nusatama as an experimental group and the SMK Nasional Padang as a control group. The research instrument used Likert Scale models, and then analyzed using Paired Samples t-test and Independent Sample t-test with SPSS version 17.00.In general the studys findings that information service with contextual teaching and learning approach is effective in improving direction of career planning student of SMK. Specifically: (1) there was a significant differences between direction of career planning in the experimental group before and after the given information service with contextual teaching and learning approach, (2) there was a significant differences between direction of career planning in the control group before and after the given conventional information service, and (3) there was a significant differences between direction of career planning in the experimental group who were information service with contextual teaching and learning approach and those in the control group who were conventional information service.
Komunikasi Interpersonal antara Orang Tua dan Anak Remaja serta Identitas Diri Remaja: Studi di Bina Keluarga Remaja Parupuk Tabing, Koto Tangah, Padang, Sumatera Barat Irianto, Agus; Aimon, Hasdi; Nirwana, Herman; Prasetya, Agung Tri
Populasi Vol 26, No 1 (2018)
Publisher : Pusat Studi Kependudukan dan Kebijakan, Universitas Gadjah Mada

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Abstract

Tujuan penelitian ini adalah mendeskripsikan komunikasi interpersonal antara orang tua dengan anak remajanya serta ketercapaian identitas remaja guna pembinaan program Bina Keluarga Remaja (BKR) oleh petugas dan konselor. Metode yang digunakan merupakan bagian dari pengembangan model ADDIE. Penyusunan tes melibatkan tiga ahli serta pelaksaannya dibantu oleh Pembina BKR dan konselor. Instrumen yang digunakan berbentuk pertanyaan dan pengukuran kepribadian yang menggunakan Skala Likert. Analisis data dilakukan dengan deskripsi dan hasil analisis menunjukkan bahwa tingkat komunikasi antara orang tua dan anak remajanya masih sedang dan cenderung rendah. Tingkat kelayakan materi berada pada kategori sangat layak. Untuk itu, perlu dikembangkan modul yang dapat digunakan oleh orang tua dan anak remajanya yang dapat membentuk komunikasi interpersonal efektif antara orang tua dan anak remajanya.
APLIKASI PROBIOTIK Aeromonas sobria A3-51 UNTUK REKAYASA NUTRISI DAN IMUNOMODULASI PERIFITON PAKAN ALAMI IKAN TILAPIA Irianto, Agus; Iriyanti, Ning
Jurnal Riset Akuakultur Vol 6, No 1 (2011): (April 2011)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

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Abstract

Probiotik Aeromonas sobria A3-51 terbukti telah mampu menekan insiden infeksi Aeromonas hydrophila dan A. salmonicida pada berbagai jenis ikan in vitro. Aplikasi melalui pakan mengalami banyak kendala oleh sebab itu dicari metode alternatif dengan menggunakannya untuk memanipulasi komponen mikroba penyusun perifiton pakan alami ikan pada substrat bambu. Dari penelitian yang dilakukan terbukti A. sobria A3-51 mampu mempengaruhi karakter biofilm perifiton serta mikroba pada lingkungan perairan ketika dilakukan di kolam tanah. Faktor lingkungan cukup berpengaruh pada kualitas air seperti penetrasi sinar matahari, pH, dan suhu. Hasil analisis proksimat menunjukkan bahwa protein perifiton cukup tinggi mencapai 22,88%-27,90% sehingga potensial digunakan sebagai bahan pakan pengganti. Adapun dari aspek imunitas, terbukti A. sobria A3-51 mampu menghambat pertumbuhan beragam bakteri Gram-negatif patogen ikan. Ketika dilakukan pengujian secara in vitro terhadap ikan tilapia, hasil belum menunjukkan bukti bahwa perifiton mampu meningkatkan derajat imun ikan tilapia berdasarkan gambaran darah