Denny Irawati
Jurusan Teknologi Hasil Hutan Fakultas Kehutanan Universitas Gadjah Mada

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Pemanfaatan Serbuk Kayu untuk Produksi Etanol dengan Perlakuan Pendahuluan Delignifikasi Menggunakan Jamur Phanerochaete Chrysosporium Irawati, Denny; Azwar, Norman Razief; Syafii, Wasrin; Artika, I Made
Jurnal Ilmu Kehutanan Vol 3, No 1 (2009)
Publisher : Jurnal Ilmu Kehutanan

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Abstract

Currently, Indonesia is in the middle ofpetroleum crisis. One ofthe alternative fuels which can be used as a petroleum substitute is ethanol. Ethanol can be produced from timber waste (sawdust). Indonesia in 2003 had timber waste potency of about 3-4 millions m3. However, ethanol production from sawdust has problems due to its lignin content. Therefore, research on bio-delignification treatment of sawdust prior to ethanol making process is required. In the present study ethanol was produced by simultaneous saccharification and fermentation (SSF) using crude cellulose from Trichoderma viride and Saccharomyces cerevisiae. The raw materials for ethanol production are sengon (Paraserianthes falcataria (L.) Nielsen syn.), meranti (Shorea sp.) and teak (Tectona grandis LIIVN.f.) sawdust after pretreatment with white rot fungi Phanerochaete chrysosporium for 10, 20 and 30 days incubation time. The yield of ethanol was between 1.65-44.83 g/1. The best combination treatment is sengon sawdust with 30 day incubation time.Keywords : sawdust, white rot fungi Phanerochaete chrysosporium, ethanol.
Pemanfaatan Serbuk Kayu untuk Produksi Etanol dengan Perlakuan Pendahuluan Delignifikasi Menggunakan Jamur Phanerochaete Chrysosporium Irawati, Denny; Azwar, Norman Razief; Syafii, Wasrin; Artika, I Made
Jurnal Ilmu Kehutanan Vol 3, No 1 (2009)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jik.925

Abstract

Utilization of Sawdust to Produce Ethanol Using Delignification Pre-treatment with White Rot Fungi Phanerochaete chrysosporiumCurrently, Indonesia is in the middle ofpetroleum crisis. One ofthe alternative fuels which can be used as a petroleum substitute is ethanol. Ethanol can be produced from timber waste (sawdust). Indonesia in 2003 had timber waste potency of about 3-4 millions m3. However, ethanol production from sawdust has problems due to its lignin content. Therefore, research on bio-delignification treatment of sawdust prior to ethanol making process is required. In the present study ethanol was produced by simultaneous saccharification and fermentation (SSF) using crude cellulose from Trichoderma viride and Saccharomyces cerevisiae. The raw materials for ethanol production are sengon (Paraserianthes falcataria (L.) Nielsen syn.), meranti (Shorea sp.) and teak (Tectona grandis LIIVN.f.) sawdust after pretreatment with white rot fungi Phanerochaete chrysosporium for 10, 20 and 30 days incubation time. The yield of ethanol was between 1.65-44.83 g/1. The best combination treatment is sengon sawdust with 30 day incubation time.
Usaha Pemanfaatan Limbah Budidaya Jamur Sebagai Bahan Baku Pembuatan Briket Untuk Energi di Kelompok Tani Jamur Sedyo Lestari Irawati, Denny; Pradipta, Naresvara Nircela; Sutapa, Johanes Pramana Gentur
Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 2, No 2 (2017): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.27033

Abstract

Mushroom farmer society Sedyo Lestari is located in the Argosari, Sedayu, Bantul. The high demand for mushrooms media (baglog) is a good business opportunities for this farmer society. But the process of baglog making requires a lot of energy in the form of firewood, especially for sterilization process of the baglog. On the other hand, the mushroom cultivation process itself remains the waste that can be used as fuel for the sterilization process. So far Sedyo Lestari farmer society members do not have the technology to used the mushroom cultivation waste as a fuel. Therefore, it is imperative to transfer knowledge of biomass briquetting technique. The method used in this activities are socialization, training, and mentoring by starting with the introduction of briquettes and briquetting technology. Subsequently made briquette-making machines followed by training briquette making. Mentoring and socialization were also made to all members of farmer society. Outcome of these activities was briquette-making machines that can be used by all members of the society as well as knowledge about the process of making briquettes. The benefit of using briquettes of mushroom cultivation media waste as fuel for subtitute the firewood can provide savings to the expenditures of farmers for buying firewood and solving the waste management problems for farmers.
Optimalisasi Penggunaan Lahan dengan Sistem Agroforestri dan Pendampingan Pascapanennya di Kelompok Tani Dusun Kemuning, Gunungkidul Kusumandari, Ambar; Irawati, Denny; Soedjoko, Sri Astuti
Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 1, No 1 (2015): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.16924

Abstract

Kemuning sub village district is located in Bunder village, Patuk, Gunung Kidul. This area is near by the Bunder forest park and Wanagama Educational Forest. Most of the people live there are farmers. As the soil is relatively unfertile, they prefer to plant trees, so they called as forest community farmers. Among the trees, they plant cassava, peanut, and corn, so it called as agroforestry. In the field, the area seems still has the opportunity that can be added to be planted. Based on this situation, in this project, the optimalization of land use was introduced to ask the farmers to plant the undergrowth species includes: zingiber, kunyit and kencur. This project was started by introducing the agroforestry system, where they can plant together both trees and non trees at the same land. The socialization was done by inviting the farmers to come to the meeting. The advantages of agroforestry was explained to them, and also the post harvesting management to produce the instant zingiber was also trained and demonstrated to the female farmers. This product is also useful for attracting the local tourist who usually come at the week end. The output of this project was the agroforestry demplot can be built in the field, so the farmers can learn and study to develope at their own land. The female farmer can also make the instant zingiber as the healthy drink.
Usaha Pemanfaatan Limbah Budidaya Jamur Sebagai Bahan Baku Pembuatan Briket Untuk Energi di Kelompok Tani Jamur Sedyo Lestari Irawati, Denny; Pradipta, Naresvara Nircela; Sutapa, Johanes Pramana Gentur
Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 2, No 2 (2017): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.27033

Abstract

Mushroom farmer society Sedyo Lestari is located in the Argosari, Sedayu, Bantul. The high demand for mushrooms media (baglog) is a good business opportunities for this farmer society. But the process of baglog making requires a lot of energy in the form of firewood, especially for sterilization process of the baglog. On the other hand, the mushroom cultivation process itself remains the waste that can be used as fuel for the sterilization process. So far Sedyo Lestari farmer society members do not have the technology to used the mushroom cultivation waste as a fuel. Therefore, it is imperative to transfer knowledge of biomass briquetting technique. The method used in this activities are socialization, training, and mentoring by starting with the introduction of briquettes and briquetting technology. Subsequently made briquette-making machines followed by training briquette making. Mentoring and socialization were also made to all members of farmer society. Outcome of these activities was briquette-making machines that can be used by all members of the society as well as knowledge about the process of making briquettes. The benefit of using briquettes of mushroom cultivation media waste as fuel for subtitute the firewood can provide savings to the expenditures of farmers for buying firewood and solving the waste management problems for farmers.
Optimalisasi Penggunaan Lahan dengan Sistem Agroforestri dan Pendampingan Pascapanennya di Kelompok Tani Dusun Kemuning, Gunungkidul Kusumandari, Ambar; Irawati, Denny; Soedjoko, Sri Astuti
Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 1, No 1 (2015): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.16924

Abstract

Kemuning sub village district is located in Bunder village, Patuk, Gunung Kidul. This area is near by the Bunder forest park and Wanagama Educational Forest. Most of the people live there are farmers. As the soil is relatively unfertile, they prefer to plant trees, so they called as forest community farmers. Among the trees, they plant cassava, peanut, and corn, so it called as agroforestry. In the field, the area seems still has the opportunity that can be added to be planted. Based on this situation, in this project, the optimalization of land use was introduced to ask the farmers to plant the undergrowth species includes: zingiber, kunyit and kencur. This project was started by introducing the agroforestry system, where they can plant together both trees and non trees at the same land. The socialization was done by inviting the farmers to come to the meeting. The advantages of agroforestry was explained to them, and also the post harvesting management to produce the instant zingiber was also trained and demonstrated to the female farmers. This product is also useful for attracting the local tourist who usually come at the week end. The output of this project was the agroforestry demplot can be built in the field, so the farmers can learn and study to develope at their own land. The female farmer can also make the instant zingiber as the healthy drink.
Association of Mushroom Cultivation and Ozonolysis as Pretreatment for Enzymatic Saccharification of Sengon(Falcataria moluccana) Sawdust Irawati, Denny; Wedatama, Soekmana; Ishiguri, Futoshi; Yokota, Shinso
Jurnal Ilmu Kehutanan Vol 12, No 1 (2018)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jik.34104

Abstract

Wood industry based on sengon (Falcataria moluccana) material has developed rapidly and cause the huge wastes. Lignocellulosic materials, such as sengon sawdust, offer a great potential as cheap and abundant feedstock for biofuels production. Ozone oxidation pretreatment was carried out on sengon wastes to improve fermentable sugar production by enzymatic saccharification. Sengon wood (SW), sengon media (SM), and sengon spent media (SMM), that was waste of media after mushroom cultivation, treated with ozone for different treatment time were enzymatically saccharified. Then the decrease of hydrolysis weight, reducing sugar yield, and monosaccharide yields were determined. Ozone treatment resulted in Klason lignin degradation in the all samples, resulting in the improvement of subsequent enzymatic saccharification. Ozone treatment with the duration more than 30 min was not suitable for SMM sample. Glucose yield after saccharification from the ozone-treated sample for 60 min was the highest among all samples in SW and SM treated, but for SMM it was 30 min. Based on these results, we concluded that mushroom cultivation can reduce the ozone time treatment and it is an effective treatment to improve sugar yield by enzymatic saccharification of sengon wastes. Gabungan Pra-perlakuan Ozonolisis dan Budidaya Jamur Sebelum Sakarifikasi Enzimatis Serbuk Kayu Sengon (Falcataria moluccana)IntisariIndustri kayu berbahan baku kayu sengon (Falcataria moluccana) saat ini tengah berkembang dengan sangat pesat dan hal ini menyebabkan timbulnya banyak limbah kayu sengon. Materi lignosellulosa, seperti limbah serbuk kayu sengon, merupakan potensi yang sangat baik sebagai bahan baku bio-fuel karena murah dan banyak tersedia. Praperlakuan dengan oksidasi ozon pada limbah kayu sengon dilakukan untuk meningkatkan produksi gula reduksi melalui proses sakarifikasi enzimatis. Serbuk sengon (SW), media sengon (SM), dan limbah media sengon (SMM), yang merupakan sisa dari media budidaya jamur, diberi perlakuan dengan ozon pada berbagai lama waktu perlakuan. Sampel yang sudah diberi perlakuan ozon tersebut kemudian dianalisis kandungan kimianya dan di-sakarifikasi secara enzimatis. Selanjutnya diukur laju hidrolisis, kadar gula pereduksi, dan kadar monosakarida. Perlakuan dengan ozon menyebabkan degradasi kadar Klason lignin di semua sampel, sehingga mengakibatkan peningkatan kemampuan sakarifikasi dari enzim. Perlakuan ozon dengan lama waktu lebih dari 30 menit tidak sesuai untuk sampel SMM. Kadar glukosa hasil sakarifikasi yang tinggi diperoleh dari sampel SW dan SM yang diberi perlakuan ozon selama 60 menit, serta SMM yang diberi perlakuan 30 menit. Berdasarkan hasil ini, dapat disimpulkan bahwa perlakuan jamur dapat mengurangi lama waktu perlakuan ozon dan praperlakuan efektif untuk meningkatkan kadar gula reduksi yang dihasilkan dari sakarifikasi enzimatis limbah kayu sengon.
Hidrolisis Media Sisa Budidaya Jamur Kuping Menggunakan Tiga Jenis Enzim Selulase Irawati, Denny
Jurnal Ilmu Kehutanan Vol 11, No 1 (2017)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jik.24900

Abstract

Jamur kuping (Auricularia polytricha) termasuk dalam kelas Basidiomycetes yang banyak dibudidayakan di Indonesia. Setelah 6-8 bulan masa pembudidayaan media budidaya jamur kuping harus diperbaharui. Sejauh ini media sisa budidaya jamur kuping belum dimanfaatkan dengan baik, bahkan limbah tersebut hanya dibuang begitu saja sebagai limbah. Hal ini sangat berbahaya karena sisa miselia yang terdapat di dalam media sisa dapat mendegradasi lebih lanjut media dan mengakibatkan pencemaran lingkungan yaitu terlepasnya gas metana ke udara. Oleh karena itu pemanfaatan sisa media budidaya kuping perlu dilakukan antara lain untuk memproduksi gula pereduksi dengan menggunakan berbagai jenis enzim selulase. Penelitian ini menggunakan media sisa budidaya jamur kuping yang berasal dari 3 jenis kayu, yaitu sengon (Falcataria moluccana), jati (Tectona grandis), dan meranti (Shorea sp.), yang dihidrolisis menggunakan 3 jenis enzim selulase yaitu Driselase, Cellulase “Onozuka” R-10, dan Meicelase. Setelah hidrolisis, kemudian diukur laju hidrolisisnya dan dianalisis kadar gula pereduksinya. Rancangan yang digunakan adalah rancangan acak lengkap dengan aras 3 x 3 serta ulangan sebanyak 3 untuk setiap perlakuan. Hasil penelitian menunjukkan bahwa media sisa budidaya jamur kuping memiliki potensi sebagai bahan baku gula pereduksi yang selanjutnya dapat difermentasi menjadi bio-etanol atau bahan kimia lainnya. Laju hidrolisis berkisar antara 1,43-21,29%, dengan kombinasi tertinggi yaitu media sisa budidaya yang terbuat dari serbuk kayu meranti dengan menggunakan enzim Meicelase. Kadar dan rendemen gula pereduksi tertinggi juga dihasilkan dari kombinasi perlakuan yang sama, yaitu berturut-turut sebesar 127,7 mg/g dan 12,8% dengan kisaran hasil antara 56,01-127,7 mg/g atau rendemen 5,6-12,8%.The Hydrolysis of Ear-Mushroom Cultivation Media Residue by Using Three Kinds of Cellulase EnzymesAbstractEar mushrooms (Auricularia polytricha) belongs to class Basidiomycetes is widely cultivated in Indonesia. After 6-8 months of cultivation, the media should be renewed. Therefore, the rest of the media is under utilized and just thrown away as a waste. This is dangerous because the residual mycelia that contained in the rest of the media may further degrade the media. This pollutes the environmental pollution due to discharge of methane into the air. Therefore, the utilization of residual ear mushroom media needs to be processed. In fact, this residue can produce a reducing-sugar using various commercial cellulase enzymes. This study used the residual media of the cultivation of mushroom derived from three types of wood, i.e. sengon (Falcataria moluccana), teak (Tectona grandis), and meranti (Shorea sp.), and then they were hydrolyzed using three types of cellulase enzymes, i.e. Driselase, Cellulase “Onozuka” R-10, and Meicelase. The hydrolysis rate was measured and the reducing-sugar yield was analyzed. The design used was completely randomized design with 3 x 3 factors and 3 replications for each treatment. The results showed that the residue of the mushroom cultivation media has the potential of reducing sugar as a raw material which can then be fermented into bio-ethanol or other chemicals. Hydrolysis rate ranged from 1.43 to 21.29%. The highest combination of residual cultivation medium was made from meranti sawdust by using Meicelase enzyme. The highest reducing sugar content yield were also resulted from the combination of the same treatment, which amounted to 127.7 mg/g or 12.8% with output range between 56.0 and 127.7 mg/g (or yield 5.6-12.8%).
PENGGUNAAN Phanerochaete chrysosporium PADA PENGOLAHAN PULP BIO-SEMI-MEKANIS KAYU TERENTANG Aprianis, Yeni; Irawati, Denny; Marsoem, Sri Nugroho
Jurnal Penelitian Hasil Hutan Vol 34, No 3 (2016): Jurnal Penelitian Hasil Hutan
Publisher : Pusat Penelitian dan Pengembangan Hasil Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2016.34.3.231-239

Abstract

Kayu terentang (Campnosperma auriculata Hook.f) memiliki berat jenis rendah (0,3), sehingga salah satu alternatif pemanfaatannya menjadi pulp adalah dengan proses semi-mekanis. Pengurangan konsumsi energi pada proses kimia-mekanis dilakukan menggunakan jamur pendegradasi lignin (Phanerochaete chrysosporium) sebagai perlakuan awal. Pengolahan pulp semi-mekanis menggunakan NaOH 4% dan masa inkubasi 0 (kontrol), 3, 4, dan 5 minggu. Hasil penelitian menunjukkan bahwa perlakuan P. chrysosporium berpengaruh nyata terhadap kehilangan berat, kandungan kimia, dan kebutuhan energi refining. Kehilangan berat serpih kayu berkisar 15,95-21,31% dan peningkatan kadar selulosa mencapai 6,77%. Inkubasi selama lima minggu menurunkan kadar lignin hingga 22,97% dan menghemat energi refining sebesar 22,7%.
Ethanol Production Using SSF Method from Calcium Hydroxide Pretreated Wood Meal Irawati, Denny; Sutapa, Johannes PG; Firmansyah, Ardi B; Mardika, Permana A; Nugroho, Farkhan W; Marsoem, Sri N
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 11, No 1 (2013): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Jurnal Ilmu dan Teknologi Kayu Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1024.398 KB)

Abstract

The objective of this research was to determine the effect of wood waste pretreatment with calcium hydroxide on the yield of ethanol. Wood waste of jati (Tectona grandhis) and sengon (Paraserianthes falcataria) were treated with 20% of CaOH2 solution for 4 days at 50, 60, and 70 °C preceding ethanol production with simultaneous saccharification and fermentation (SSF) method. In the present SSF process, saccharification and fermentation were respectively carried out by the use of cellulase and Saccharomyces cerevisiae in a shaker incubator with 12 rpm at 30 °C for 72 hours. The resulting ethanol was then analyzed using gas chromatography. The chemical compounds of woods (i.e. extractive, holocellulose, alpha-cellulose, pentose and lignin) were also evaluated. It was found that increasing the temperature of alkaline pretreatment increased ethanol yield for both sengon and jati wood wastes. The ethanol yield was in the range of 0.027-0.054%. CaOH2 pretreatment at 70 oC of sengon wood wastes resulted in the highest yield. At elevated temperature, the pretreatment of wood wastes decreased the content of extractive, holocellulose, lignin and pentosan of sengon and jati wood wastes. These were in the contrary to that of increased alpha cellulose content.Key words: calcium hidroxyde, enzymatic saccharification, ethanol, Paraserianthes falcataria, Tectona grandis