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Penentuan Lama Sulfonasi pada Proses Produksi Surfaktan Mes untuk Aplikasi EOR Rival, Mira; Irawadi, Tun Tedja; Suryani, Ani; Setyaningsih, Dwi; Hambali, Erliza
Jurnal Ilmu Pertanian Indonesia Vol 16, No 1 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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For producing oil remains that remained at old oil wells (mature field), a method of advanced oilacquirement improvement known as an Enhanced Oil Recovery (EOR) should be applied. Surfactant plays animportant role in EOR process by reducing interfacial tension (1FT), altering wettability, reducing oil viscosity,and stabilizing dispersion to facilitate the process of oil jetting from reservoir to production well. To optimallycleanse oil that still remained a surfactant compatible with formation water and reservoir is needed. This studywas conducted to get the best time of sulfonation process for producing MES surfactant with lower interfacialtension for EOR application. Results showed that the best times of sulfonation process with lower interfacialtension value were 3 and 4 hours.Keywords: Surfactant, MES, sulfonation time, interfacial tension, EOR.
SINTESIS PATI SAGU IKATAN SILANG FOSFAT BERDERAJAT SUBSTITUSI FOSFAT TINGGI DALAM SUASANA ASAM [Synthesis of Cross-Linked Sago Starch Phosphate with the Highest Degree of Substitution of Phosphate Under Acidic Condition] Romengga, Jorion; Irawadi, Tun Tedja; Djulaika, Retno; Muntamah, .; Zakaria, Ahmad
Jurnal Teknologi Dan Industri Pangan Vol 22, No 2 (2011): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/4251

Abstract

SINTESIS PATI SAGU IKATAN SILANG FOSFAT BERDERAJAT SUBSTITUSI FOSFAT TINGGI DALAM SUASANA ASAM [Synthesis of Cross-Linked Sago Starch Phosphate with the Highest Degree of Substitution of Phosphate Under Acidic Condition] Jorion Romengga*, Tun Tedja Irawadi, Retno Djulaika, Muntamah, dan Ahmad Zakaria Fakultas Matematika dan Ilmu Pengetahuan Alam, Departemen Kimia, Institut Pertanian Bogor   Diterima 23 Februari 2011 / Disetujui 10 Oktober 2011 ABSTRACT   Cross-linked sago starch phosphate (SgP) with high phosphorus contents was successfully synthesized by reacting sago with a mixture of primary and secondary sodium phosphates under acidic condition. The experimental variables investigated include pH, temperature, reaction time, and mixture rate. The physicochemical properties evaluated were moisture, swelling power, water binding capacity, transmittance (%T) and percent amylose (%Am), while the pasting properties examined were pasting time, pasting temperature, viscosity at peak, final, and setback. The granule structure was observed by scanning electron microscope and X-ray diffraction. The results showed that the maximum degree of phosphate substitution was obtained at pH of 6.50, 40°C, 20 minutes of reaction time and 300 rpm of mixing rate. The physicochemical (%T and %Am) and pasting (viscosity at peak, final, and setback) properties of SgP were significantly different (P
BIOADSORPSI Hg(II) OLEH PATI SAGU TAUT SILANG FOSFAT [Bioadsorption of Hg(II) by Crosslinked Sago Starch Phosphate] Romengga, Jorion; Irawadi, Tun Tedja; Sugiarti, Sr
Jurnal Teknologi Dan Industri Pangan Vol 23, No 2 (2012): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/6151

Abstract

BIOADSORPSI Hg(II) OLEH PATI SAGU TAUT SILANG FOSFAT [Bioadsorption of Hg(II) by Crosslinked Sago Starch Phosphate] Jorion Romengga1)*, Tun Tedja Irawadi2) dan Sri Sugiarti2) 1) Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Tanjungpura, Pontianak 2) Departemen Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor   Diterima 15 September 2011 / Disetujui 09 Juli 2012 ABSTRACT   Crosslinked-sago-starch-phosphate (SgP) has been successfully synthesized from native sago starch (Sg) and Na2HPO4-NaH2PO4 in an acidic condition. The compound was designed as bioadsorbent for removing Hg(II) inside human digestion tract as shown by in vitro test. The bioadsorption followed pseudo-second order of reaction kinetic and Freundlich equation as chemisorption. As a result, 21% of Hg(II) was removed at pH of 6.80 and reached the isothermal equilibrium of the bioadsorption at pH of 5.80 and 8.60 for 29.95% and 31.39%, respectively. The result showed that SgP is more feasible than activated carbon to be used as bioadsorbent in removing Hg(II) in human digestion tract as proved by in vitro system.   Keywords: bioadsorption, phosphate crosslinked, Hg(II), phosphate, sago starch ABSTRAK   Pati sagu taut silang fosfat (SgP) telah berhasil disintesis dari pati sagu natif (Sg) dan Na2HPO4-NaH2PO4 dalam suasana asam. SgP tersebut didesain sebagai bioadsorben untuk menjerap Hg(II) yang terdapat dalam saluran pencernaan manusia yang dilakukan secara in vitro. Proses bioadsorpsi tersebut memenuhi kinetika reaksi orde kedua semu dan persamaan Freundlich sebagai kemisorpsi. Adapun hasilnya menunjukkan bahwa 21% ion Hg(II) telah dijerap pada pH 6,80 dan mencapai kesetimbangan isotermal bioadsorpsi pada pH 5,80-8,60 sebesar 29,95% dan 31,39%, secara berturut-turut. Hal ini menunjukkan bahwa SgP lebih berguna daripada karbon aktif sebagai bioadsorben umum yang digunakan untuk menjerap Hg(II) pada saluran pencernaan manusia yang dibuktikan secara in vitro.   Kata kunci: bioadsorpsi, taut silang fosfat, Hg(II), fosfat, pati sagu  
Isolasi Dan Penapisan Aktinomisetes Laut Penghasil Antimikroba Sunaryanto, Rofiq; Marwoto, Bambang; Irawadi, Tun Tedja; Hartoto, Liesbetini
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 14, No 2 (2009): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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 Telah diiakukan isolasi dan penapisan aktinomisetes laut yang mampu menghasilkan senyawa antimikroba. Isolasi diiakukan ditiga tempat berbeda yaitu di Pantai Barat Banten, Pantai Utara Cirebon, dan Pantai Selatan Yogyakarta. Isolasi dilakukan dengan dua metode pre-treatment yaitu dengan metode pengasaman dan metode pemanasan. Dari hasil isolasi diperolehjumlah total isolat sebanyak 50 isolat. Setelah diiakukan penapisan diperoleh 4 isolat yang mampu menghambat Eschereschia coli, 5 isolat mampu menghambat Streptococcus aereus, 4 isolat mampu menghambat Bacillus subtilis, 4 isolat mampu menghambat Pseudomonas aeroginosa, 5 isolat mampu menghambat Candida albican, dan 4 isolat mampu menghambatAspergillus niger. Hasil identifikasi morfologi dan DNA dari salah satu isolat yang memiliki aktivitas antibakteri paling kuat (isolat A11) adalah Streptomyces sp. Secara morfologi isolat A11 memiliki hifa yang bercabang dengan kantong spora pada ujung hifa. Minimum Inhibition Concentration (MIC) isolat A11 terhadap Bacillus subtilis sebesar 120,86 μg/ml.Kata kunci: Isolasi, penapisan, aktinomisetes laut, antimikrobaIsolation and screening of antimicrobial-producing marine actinomycetes has been conducted on isolates taken from West Banten, North Cirebon, and South Yogyakarta Coasts. Two methods pretreatments were applied i.e. acid and heat shock method. The research 50 isolates. The screening reavealed four isolates which has ability to inhibi Eschereschia coli, 5 isolates could inhibited Streptococcus aereus, 4 isolates could inhibited Bacillus subtilis, 4 isolates could inhibited Pseudomonas aeroginosa, 5 isolats could inhibited Candida albican, and 4 isolatescould inhibited Aspergillus niger. Result of  identification morphology and DNA of isolate A11 it’s Streptomyces sp. Morphology of isolate A11 haves branching hyphae with spore sack at the end of hyphae. The Minimum Inhibition Concentration (MIC) of isolate A11 to Bacillus subtilis was 120,86 μg /ml.Key words: Isolation, Screening, Marine Actinomycetes, Antimicrobial.
PERUBAHAN SUHU PIROLISIS TERHADAP STRUKTUR KIMIA ASAP CAIR DARI SERBUK GERGAJI KAYU PINUS WIJAYA, Mohammad; NOOR, Erliza; IRAWADI, Tun Tedja; PARI, Gustan
Jurnal Ilmu dan Teknologi Hasil Hutan Vol 1, No 2 (2008): Jurnal Ilmu Teknologi Hasil Hutan
Publisher : Departemen Hasil Hutan, IPB

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The purpose of this research is to study the effect of  pyrolysis temperature to the wood vinegar chemical composition by be produced wood vinegar, charcoal, biofuel  etc. The variation of pyrolysis  temperatures were 110, 200, 300, 400 and 500 °C at 5 hours. That pine wood vinegar yield obtained were 13.80%, 16.12%, 11.99%, 15.51% and 0.90 %.  Analysis  DTA (Diferential Thermal Analysis)  showed thermal decompotition occurs at  227, 320.2 and 349.7 °C.  Identification of GC-MS of pine sawdust could provide compounds that mostly derived from acid group and was dominated by acetic acid produced was 31.65%. At distillate of teak smokes was obtained three yield of liquid smokes resulted from conversion of pine wood  wastes can be utilized to preserve fishes.   Keywords :  Pine sawdust,  pyrolysis, wood vinegar, and preserve  fishes
Hydrolysis Methods of Starch for Culture Media of Bacillus sp. BMN14 Producing Biosurfactant Lipopeptide Richana, Nur; Suryani, Ani; Makagiansar, Helena Yusuf; Irawadi, Tun Tedja
Jurnal Mikrobiologi Indonesia Vol 5, No 2 (2000): JURNAL MIKROBIOLOGI INDONESIA
Publisher : Jurnal Mikrobiologi Indonesia

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Ezymatic and acid hydrolysis of starch for culture media of Bacillus sp. BMN14 producing lipopeptide blosurfactantwere studied using cassava, arrowroot and sago starch. These starch bydrolysates were used as glukose substitute Inthe were to culture media of biosurfactant.producing bacteria. Starch hydrolyzed from arrowroot 73.08-83.5% andcassava 80.0-87.98% yielded glucose higher than sago 62.5-64.5%. Capability of the bacteria to produce biosurfactantrepresented as the amount of acid precipitate 0.78 g/l and surface tension of the broth 33.6 mN/rn in the culturemedium contained enzymatic hydrolyzed-starch.
The Process of Xylanase Production from Bacillus pumilus RXAIII-5 RICHANA, NUR; IRAWADI, TUN TEDJA; NUR, M. ANWAR; SAILAH, ILLAH; SYAMSU, KHASWAR
Microbiology Indonesia Vol 1, No 2 (2007): August 2007
Publisher : Indonesian Society for microbiology

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The optimum conditions for the growth of Bacillus pumilus RXAIII-5 (a potential xylanase producer) were sought, these included temperature, pH, aeration, and agitation of the culture batch. Afterwards a mathematical model based on the parameter of cultivation kinetics was formulated. At the same time, the rheology of the fluid used for bacterial cultivation in a bioreactor was studied. The data obtained was used for estimating the ‘scaling up’ of enzyme production. The results of the study indicate that the optimum condition for processing in 50 ml Erlenmeyer flask are used temperature of 35 oC (308oK), pH 7, and an agitation rate of 140 rpm. The highest xylanase activity and its specific activity are 297.132 U.ml-1 and 655.32 U.g-1protein, respectively. Subsequent experiments in a bioreactor using all of the experiment parameters mentioned above, except for the agitation rate, shows that the results are as follows. The highest specific growth was at 0.082 hour-1 at an aeration and agitation rate of 0.5 vvm and 150 rpm, respectively. Based on the data of the cultivation kinetics, the optimum conditions for the fermentation in Biostat 2L-bioreactor is 1 vvm and 200 rpm of aeration and agitation, respectively . The efficiency of substrate (Yp/s) and of cell biomass (Yp/x) to produce xylanase is 50.744 U.g-1 and 43.906 U.g-1, respectively. The efficiency of substrate to cell production (Yx/s) is 1.178g.g-1. The liquid cultivation-medium has non-Newtonian properties. Based on a mathematical model it is found that the consistency index (k constant) and index of liquid behavior (n value) are 0.179 g.cm-1.second-1 and 0.3212, respectively. Becouse the value of 0
Isolation and Characterization of Antimicrobial Substance from Marine Streptomyces sp. SUNARYANTO, ROFIQ; MARWOTO, BAMBANG; IRAWADI, TUN TEDJA; HARTOTO, LIESBETINI
Microbiology Indonesia Vol 4, No 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

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Isolation and purification of antimicrobial active substance produced by marine Actinomycetes has been carried out.  Marine sediment samples were obtained from six different places at Banten West Coast.  Isolation was conducted using two pretreatment methods,  acid and heat shock pre-treatment.  A total of 29 Actinomycetes isolates were obtained from the various sediment samples collected, then tested for antimicrobial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Pseudomonas aeruginosa ATCC27853, Bacillus subtilis ATCC 66923, Candida albicans BIOMCC00122, and Aspergillus niger BIOMCC00134.  Among the isolates, isolate A11 was the most activity to Gram-positive and Gram-negative bacteria, and morphological observation and identification using 16S rRNA showed that the isolate was similar to Streptomyces sp.  Production of active compound from A11 isolate used yeast peptone medium.  Purification of active compounds was carried out using silica-gel-column chromatography and preparative HPLC.  A single peak of active compounds was detected by HPLC, which showed a retention time of 8.35 min and maximum absorbance in UV visible at 210 nm and 274.5 nm respectively.
Cyclo (Tyrosyl-Prolyl) Produced by Streptomyces sp.: Bioactivity and Molecular Structure Elucidation SUNARYANTO, ROFIQ; MARWOTO, BAMBANG; HARTOTO, LIESBETINI; IRAWADI, TUN TEDJA
Microbiology Indonesia Vol 5, No 2 (2011): June 2011
Publisher : Indonesian Society for microbiology

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Determination of bioactivity by minimum inhibitory concentration (MIC) methods and molecular structure identification of antibiotic produced by Streptomyces sp. have been carried out. The antibiotic was produced by liquid culture using Streptomyces sp. isolate. Purification of antibiotic was carried out by silica gel column chromatography and preparative HPLC. Molecular structure identification was carried out using ESI-MS, 1H NMR, 13C NMR, and 13C DEPT NMR. Pure antibiotic showed inhibition activity to Gram-negative and Gram-positive bacteria. MIC to Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC27853 , Staphylococcus aureus ATCC25923, and Bacillus subtilis ATCC66923 were 27.0, 68.7, 80.2, and 73.7 μg mL-1 , respectively. Identification using ESI-MS showed that the molecular weight of this antibiotic was 260 g mol-1 , and molecular formula was C14H16N2O3 . Elucidation of molecular structure using 1HNMR, 13C NMR, and 13C DEPT NMRshowed that antibiotic was cyclo(tyrosyl-prolyl).
KAJIAN MANFAAT EKONOMIS PENERAPAN KONSEP PRODUKSI BERSIH PADA INDUSTRI KARET REMAH BERBASIS KARET RAKYAT Utomo, Tanto Pratondo; Fauzi, Anas Miftah; Irawadi, Tun Tedja; Romli, Muhammad; Aman, Amril; Honggokusumo, Suharto
Majalah Ilmiah Ekonomi Komputer 2007: Majalah Ilmiah Ekonomi Komputer Edisi Agustus
Publisher : Majalah Ilmiah Ekonomi Komputer

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Tujuan penelitian ini adalah menganalisis manfaat ekonomis penerapan konsep produksi bersihberdasarkan altematif terpilih untuk perbalkan proses pada industri karet remah yang dapal meningkatkanefisiensi dan mengurangi resiko pencemaran dan dapat diterapkan pada penyedia bahan baku (petani karet,KUD, dan pedagang pengumpul) dan pengolahan bokar menjadi karet remah (pabrik karet remah). Hasilpenelitian menunjukkan bahwa penerapan konsep produksi bersih pada industri karet remah berbasis karetrakyat yang diterapkan pada tahap penyediaan bahan baku dan pada tahap pengolahan bokar menjadi karetremah menghasilkan keuntungan ekonomis dengan (1) penghematan air sebanyak 18,5 m3110n karet kering;(2) penghematan energi senilai Rp. 7.91011on karet kering; (3) tidak diperlukan investasi untuk peralatanpenghilangan bau (malodour); (4) dihindari terjadinya kerugian akibat proses penggantungan selama 14 harisenilai Rp. 70/kg bokar; dan (5) tahapan proses pengolahan bokar menjadi karet remah lebih singkat dengantidak digunakannya mesin hammer-mills. Dampak ekonomis yang bersifat menambah biaya adalah (1)diperlukan investasi tambahan untuk resirkulasi air; (2) investasi fasilitas penggilingan bokar; dan (3) investasibiaya pengolahan Iimbah berupa serum hasil pengpresan bokar pada tingkat petani karet.Kata kunci: produksi bersih, bokar, karet remah, manfaat ekonomis