Luthfia Indriyani
Cancer Chemoprevention Research Center Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta.
Articles
2
Documents
Combination of Tangeretin and 5-Fluorouracil Modulates Cell Cycle and Induce Apoptosis on Widr Cells

Indonesian Journal of Cancer Chemoprevention Vol 3, No 1 (2012)
Publisher : Indonesian Research Gateway

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Abstract

Co-chemotherapeutics approaches are increasing in cancer treatment in order mainly to suppress the resistance phenomenon of cancer treatment and to enhance the cytotoxic effect of the main chemotherapeutics agent. Tangeretin has been known to have cytotoxic effect to some cancer cells through some pathways in the cells. To explore the potential effect of tangeretin as co-chemotherapeutics agent this research was subjected to study the cytotoxic  effect of tangeretin in combination with 5-Fluoro Uracil (5-FU) on WiDR colon cancer cells covering the modulation of cell cycle and apoptosis induction. Cytotoxic effect was examined by using MTT assay while apoptosis induction was determined by annexin-V flowcytometry. Under MTT assay, tangeretin showed weak cytotoxic activity on the cells. However, tangeretin significantly enhanced the cytotoxic effect of 5-FU on the cells. This co-chemotherapeutics effect likely correlated with cell cycle modulation effect, especially in inducing polyploidy phenomenon as expressed in the flowcytometric graph of the DNA content. This combination also increased apoptosis induction. These result suggest that tangeretin is potential to be developed as co-chemotherapeutic agent for 5-FU on colon cancer and further molecular mechanism need to be exploredKeywords : Tangeretin, 5-Fluorourasil, WiDr, cell cycle, apoptosis

INDUKSI APOPTOSIS EKSTRAK ETANOL CIPLUKAN (Physalis angulata L.) PADA SEL KANKER LEHER RAHIM HeLa MELALUI PENEKANAN EKSPRESI Bel-2

Jurnal Bahan Alam Indonesia Vol 7, No 7 (2011)
Publisher : Indonesian Research Gateway

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Abstract

The incidence of cervical cancer continue to rise encourage a variety of studies to explore the plant as chemopreventive. Ciplukan (Physalis angulata L.) is one of potential plant as chemopreventive agent by regulating cell proliferation, cell cycle and apoptosis. In this study the potential effect of ciplukan ethanolic extract as inducers of apoptosis in human cervical carcinoma HeLa cell line wil be futher investigated. Induction of apoptosis was evaluated by Double Staining methods and Immunocytochemistry. Apoptosis observations qualitatively indicate the occurrence of early apoptosis marked by orange fluorescence and apoptosis bodies because of cell fragmentation. Then the expression of Bcl-2 was observed to know molecular mechanism of apoptosis. Immunocytochemistry experiment showed that Bcl-2 as antiapoptosis protein was inhibited in comparison with control cells. These data indicated that ciplukan ethanolic extract (P.angulata L.) could induced the apoptosis Hela cell line by downregulation of Bcl-2. Study of ciplukan shoud be developed to support ciplukan to be effective anticancer agents.