AGUSTIN INDRAWATI
Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner FKH IPB

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The Comparison of Streptococcus agalactiae Isolated from Fish and Bovine using Multilocus Sequence Typing LUSIASTUTI, ANGELA MARIANA; SEEGER, HELGA; INDRAWATI, AGUSTIN; ZSCHÖCK, MICHAEL
HAYATI Journal of Biosciences Vol 20, No 4 (2013): December 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.20.4.157-162

Abstract

Multilocus sequence typing (MLST) has greater utility for determining the recent ancestral lineage and the relatedness of individual strains. Group B streptococci (GBS) is one of the major causes of subclinical mastitis of dairy cattle in several countries. GBS also sporadically causes epizootic infections in fish. The aim of this study was to compare the evolutionary lineage of fish and bovine isolates in relation to the S. agalactiae global population as a whole by comparing the MLST profiles. Twenty S. agalactiae isolates were obtained from dairy cattle and fish. PCR products were amplified with seven different oligonucleotide primer pairs designed from the NEM316 GBS genome sequence. Clone complexes demonstrated that bovine and fish isolates were separate populations. These findings lead us to conclude that fish S. agalactiae is not a zoonotic agent for bovine. MLST could help clarify the emergence of pathogenic clones and to decide whether the host acts as a reservoir for another pathogenic lineage.
Uji Patogenisitas Zoospora Kapang Lagenidium giganteum terhadap Larva Instar-2 Nyamuk Aedes aegypti Skala Laboratorium (PATHOGENICITY TEST OF ZOOSPORA LAGENIDIUM GIGANTEUM FUNGI AGAINST AEDES AEGYPTI LARVAE 2nd UNDER LABORATORY CONDITION) Indrawati, Agustin; Sudarwanto, Mirnawati; Tampubolon, Mangaraja Pidoli; Soejoedono, Retno Damayanti; Wibawan, I Wayan Teguh
Jurnal Veteriner Vol 12, No 1 (2011)
Publisher : Jurnal Veteriner

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Abstract

Dengue Haemorrhagic fever (DHF) is one of fearsome diseases in society. Incidence of the disease isincreasing. Dengue fever is caused by dengue virus and transmitted by Aedes aegypti mosquito vector.Various chemical controls have been conducted to prevent the spread of the disease, but active contents ofthe chemical controlling substances are suspected causing many negative effect, in environment, such asvector resistance, death of non target living creatures, and environmental contamination.This researchobjective was to find an alternative solution in order to control the dengue vector by using entomopathogenicfungi as biological control agent. This research was conducted by isolation and identification of fungiinfecting mosquito larvae. Macroscopic observation revealed that one of the nine isolation products wasLagenidium giganteum. The effectiveness test in laboratory showed the zoospore LD50 to Ae.aegypti larvaeof instar 2nd was 2,35 x 106 zoospore/ml, while the LD95 value was 1,35 x 107 zoospore/ml. The oosporeeffectiveness test showed LD50 was 6,7 x 102 oospore/ml and LD95 was 1,94 x 103 oospore/ml. Using LPCBdye and blue tolouidin 2,5%, the infection mechanism of L.giganteum fungi in Ae.aegypti mosquito larvawas detected. The research is concluded that the entomophatogen fungi L. giganteum was very prospectiveto be used as a biological control agent against vector of DHF.
Brucella ceti : THE Threat of Emerging Zoonoses in Indo Pacific Bottle Nose (Tursiops aduncus) Indrawati, Agustin; Affif, Usama
Jurnal Sain Veteriner Vol 30, No 2 (2012)
Publisher : Fakultas Kedokteran Hewan

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Abstract

Incidence of Brucellosis in marine mammals in the world is  found lately particularly in Penniped and Cetacea. Indonesia is an archipelago that is rich with its Wildlife and one of them is Indo Pacific bottle nose dolphins (Tursiops aduncus). In this research, Indo Pasific bottle nose dolphins (Tursiops aduncus) were used deriving from captive of marine mammal conservation in Indonesia PT Wersut Seguni. All of them were in good condition. A total of 23 blood samples collected from the caudal vein, located above the tail fin. Blood samples was taken as Aseptic as possible. Screening test against Brucella spp was done by Rose Bengal Test (RBT) and Rapid Test B. Brucella. One sample out of 23 samples had  antibody positif against Brucella spp (4%). The results of this research concluded that Brucella spp found is likely to be Brucella Ceti . It is also found in Indonesia territorial  and  in the Indo Pacific bottle nose dolphins (Tursiops aduncus). We hope that this finding will do raise our awerness to emerging zoonoses.Keywords: brucellosis, Brucella ceti, RBT, Rapid test, Tursiops aduncus 
Monoclonal antibodies to human IgM Sjahrurachman, Agus; Indrawati, Agustin; Ernawati, Betty; Ibrabim, Fera; Mardiastuti, Mardiastuti
Medical Journal of Indonesia Vol 8, No 4 (1999): October-December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (512.461 KB) | DOI: 10.13181/mji.v8i4.714

Abstract

[no abstract available]
Studi Sistem Respirasi dan Kajian Mikrobiologis Lumba-lumba Hidung Botol Indo Pasifik (Tursiops aduncus) dari Perairan Laut Jawa Mulyani, Guntari Titik; Fibrianto, Yuda Heru; Budipitojo, Teguh; Indrawati, Agustin
ACTA VETERINARIA INDONESIANA - Indonesian Veterinary Journal Vol 2, No 1 (2014): Januari 2014
Publisher : Bogor Agricultural University

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Abstract

Lumba-lumba hidung botol (bottlenose dolphin) adalah spesies lumba-lumba yang paling umum danpaling dikenal orang. Gangguan sistem respirasi pada lumba-lumba sering dijumpai, sementara jenisbakteri yang sering menyerang lumba-lumba dari perairan Laut Jawa belum pernah diteliti. Penelitianini bertujuan untuk mempelajari pemeriksaan klinis sistem respirasi lumba-lumba dan melakukan isolasibakteri serta jamur pada sistem respirasi bottlenose dolphin (Tursiops aduncus) dari Perairan Laut Jawa.Penelitian dilakukan dengan studi pustaka, pemeriksaan klinis serta isolasi sampel sistem respirasi. Swabdeep blowhole dilakukan terhadap sepuluh ekor bottlenose dolphin di PT. Wersut Seguni Indonesia.Sampel dikirim ke laboratorium Mikrobiologi FKH UGM untuk isolasi dan identifikasi terhadap bakteridan jamur. Hasil penelitian menunjukan bahwa pemeriksaan klinis lumba-lumba hanya dapat dilakukansecara inspeksi. Hasil isolasi ditemukan bahwa 5 dari 10 lumba-lumba (50%) positif Staphylococcus aureus,sedangkan jamur tidak ditemukan. Berdasarkan hasil penelitian, disimpulkan bahwa pemeriksaan klinislumba-lumba dapat dilakukan dengan cara inspeksi terhadap blowhole, tingkah laku hewan, adanya“chuff” atau “honk”, frekuensi respirasi, sosialisasi hewan dan posisi hewan saat berenang. BakteriStaphylococcus aureus dijumpai pada sistem pernafasan lumba-lumba hidung botol dari perairan LautJawa di PT. Wersut Seguni Indonesia.
Studi Sistem Respirasi dan Kajian Mikrobiologis Lumba-lumba Hidung Botol Indo Pasifik (Tursiops aduncus) dari Perairan Laut Jawa Mulyani, Guntari Titik; Fibrianto, Yuda Heru; Budipitojo, Teguh; Indrawati, Agustin
Acta VETERINARIA Indonesiana Vol 2, No 1 (2014): Januari 2014
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.2.1.7-11

Abstract

Lumba-lumba hidung botol (bottlenose dolphin) adalah spesies lumba-lumba yang paling umum danpaling dikenal orang. Gangguan sistem respirasi pada lumba-lumba sering dijumpai, sementara jenisbakteri yang sering menyerang lumba-lumba dari perairan Laut Jawa belum pernah diteliti. Penelitianini bertujuan untuk mempelajari pemeriksaan klinis sistem respirasi lumba-lumba dan melakukan isolasibakteri serta jamur pada sistem respirasi bottlenose dolphin (Tursiops aduncus) dari Perairan Laut Jawa.Penelitian dilakukan dengan studi pustaka, pemeriksaan klinis serta isolasi sampel sistem respirasi. Swabdeep blowhole dilakukan terhadap sepuluh ekor bottlenose dolphin di PT. Wersut Seguni Indonesia.Sampel dikirim ke laboratorium Mikrobiologi FKH UGM untuk isolasi dan identifikasi terhadap bakteridan jamur. Hasil penelitian menunjukan bahwa pemeriksaan klinis lumba-lumba hanya dapat dilakukansecara inspeksi. Hasil isolasi ditemukan bahwa 5 dari 10 lumba-lumba (50%) positif Staphylococcus aureus,sedangkan jamur tidak ditemukan. Berdasarkan hasil penelitian, disimpulkan bahwa pemeriksaan klinislumba-lumba dapat dilakukan dengan cara inspeksi terhadap blowhole, tingkah laku hewan, adanya?chuff? atau ?honk?, frekuensi respirasi, sosialisasi hewan dan posisi hewan saat berenang. BakteriStaphylococcus aureus dijumpai pada sistem pernafasan lumba-lumba hidung botol dari perairan LautJawa di PT. Wersut Seguni Indonesia.
Deteksi Residu Hormon Trenbolon Asetat pada Sapi Siap Potong Impor asal Australia Danial, Rifky; Latif, Hadri; Indrawati, Agustin
Acta VETERINARIA Indonesiana Vol 3, No 2 (2015): Juli 2015
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.3.2.70-76

Abstract

Trenbolon asetat (TBA) merupakan hormon penggertak pertumbuhan yang diimplankan ke sapi untuk meningkatkan berat badan dan mengefisiensi konversi pakan. Penggunaan TBA dapat meninggalkan residu dalam urin dan dapat menyebabkan efek negatif. Tujuan dari penelitian ini adalah untuk menganalisis keberadaan residu TBA dalam urin sapi siap potong impor dari Australia. Ukuran sampel dihitung dengan menggunakan rumus deteksi penyakit dan sampel dipilih secara acak. Sebanyak 60 sampel dianalisis menggunakan enzim-linked immunosorbent assay (ELISA). Tes menunjukkan bahwa sebanyak 100% urin sapi siap potong dari Australia mengandung residu TBA dengan konsentrasi yang bervariasi. Konsentrasi residu TBA < 2 part per billion (ppb) terdeteksi pada 37 sampel (61,67%), konsentrasi residu TBA 2-4 ppb terdeteksi pada 7 sampel (7%), dan konsentrasi residu TBA > 4 ppb terdeteksi pada 16 sampel (26,67%). Hasil positif menunjukkan bahwa sapi potong asal Australia mengandung residu hormon trenbolon asetat (TBA).Kata kunci: ELISA, residu, sapi potong impor, trenbolon asetat, urin (Detection of Trenbolone Acetate Hormone Residues in Imported Slaughter Cattle from Australia)Trenbolone acetate (TBA) is a growth hormone promoter which is implanted into cattle to increase weight gain and feed conversion efficiency. The use of TBA can leave residue in urine and may cause negative effects. The objective of this research was to analyze the presence of the TBA residue in imported slaughter cattle urine from Australia. Cattle urine samples were collected from Animal Quarantine Installation. Sample size was calculated using the formula of detect disease and selected by random sampling. A total of 60 samples of cattle urine were analyzed for level of trenbolone acetate residues by using enzyme-linked immunosorbent assay (ELISA) method. The test showed that positive results in all of urine samples (100%) of slaughter cattle imported from Australia with variation in TBA residues concentrations. The concentration of residual TBA < 2 ppb were detected in 37 samples (61.67%), the residual concentration of TBA 2-4 ppb were detected in 7 samples (7%), and the concentration of residual TBA > 4 ppb were detected in 16 samples (26.67%). Total of 60 urine samples contained TBA residues. The presence of TBA residues with concentration above 4 ppb was 16 samples (26.7%). Positive results in the samples was indicated the Australian cattle contains trenbolone acetate (TBA) residue.Keywords: ELISA, residue, imported slaughter cattle, trenbolone acetate, urine
Deteksi Salmonella spp. pada Telur Ayam Konsumsi yang Dilalulintaskan melalui Pelabuhan Tenau Kupang Nugroho, Susanto; Purnawarman, Trioso; Indrawati, Agustin
Acta VETERINARIA Indonesiana Vol 3, No 1 (2015): Januari 2015
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.3.1.16-22

Abstract

Salmonelosis adalah salah satu penyakit food-borne bakterial zoonotik yang paling penting di seluruh dunia. Salmonella spp. adalah penyebab salmonelosis akibat konsumsi makanan berbahan dasar unggas dan produk unggas yang terkontaminasi. Unggas dan telur ayam dianggap merupakan salah satu reservoir Salmonella spp. yang paling penting. Salmonella spp. ditularkan melalui rantai makanan dan akhirnya menular ke manusia. Meningkatkan keamanan produk unggas dengan cara deteksi dini terhadap food-borne patogen merupakan komponen penting untuk membatasi kontaminasi Salmonella spp.. Metode deteksi dan identifikasi Salmonella spp. merupakan strategi yang dirancang untuk mencegah kontaminasi unggas dan produk unggas. Tujuan dari penelitian ini adalah untuk mendeteksi Salmonella spp. dari telur ayam yang berasal dari 4 pengirim telur menggunakan metode konvensional. Jumlah sampel dihitung dengan menggunakan rumus menduga prevalensi dan diambil menggunakan metode acak berlapis. Analisis data hasil positif Salmonella spp. dilakukan secara deskriptif. Berdasarkan hasil pengujian dari 270 sampel, 5 pengujian dengan metode konvensional positif Salmonella spp.. Berdasarkan hasil pengujian positif kontaminasi Salmonella spp. pada telur ayam maka diperlukan evaluasi terhadap pengiriman telur ayam konsumsi antar pulau.Kata kunci: kontaminasi, Salmonella spp., metode konvensional, telur ayam. (Detection of Salmonella spp. in Commercial Hen Eggs Entering through Tenau Port Kupang)Salmonellosis is one of the most important food-borne bacterial zoonotic diseases worldwide. Salmonella spp. are causative agent of salmonellosis associated with contaminated commercial poultry and poultry product. Poultry and eggs are considered one of the most important Salmonella spp. reservoirs. Salmonella spp. were able to pass through the food chain and ultimately transmitted to humans. Improving safety of poultry products by early detection of food-borne pathogens would be considered an important component for limiting exposure to Salmonella contamination. Detection and identification method for Salmonella spp. are considered to be an important component of strategies designed to prevent poultry and poultry product. The aims of the study were to detect Salmonella spp. from hen eggs collected from 4 exporters using conventional method. Samples size were calculated using estimates prevalence formula and selected by stratified random sampling. Data regarding the proportion of Salmonella spp. positive samples were analyzed descriptively. 270 samples, 5 test by conventional method were positive Salmonella spp.. According of positive test results Salmonella spp. contamination in hen eggs was necessary to evaluate the delivery of commercial hen eggs between islands.Keywords: conventional methods, hen eggs, Salmonella spp. contamination.
Deteksi Penyakit Bovine Viral Diarrhea pada Sapi Potong Impor melalui Pelabuhan Tanjung Priok Primawidyawan, Aditya; Indrawati, Agustin; Lukman, Denny Widaya
Acta VETERINARIA Indonesiana Vol 4, No 1 (2016): Januari 2016
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.4.1.7-13

Abstract

Penelitian ini bertujuan untuk melakukan suatu kajian serologis tentang penyakit bovine viral diarrhea (BVD) dan mendeteksi adanya kaitan pemeliharaan kandang sebagai faktor risiko sumber penularan penyakit BVD pada sapi potong impor. Pengujian screening awal mengggunakan ELISA (enzyme linked immunosorbant assay) Antibodi BVD terhadap 100 sampel serum darah sapi, dan ditemukan 63 positif terhadap adanya antibodi anti BVD. Selanjutnya dilakukan pengujian lanjutan ELISA Antigen BVD dan hasilnya seluruh sampel negatif terhadap Antigen BVD. Hasil positif uji ELISA terhadap antibodi BVD mengindikasikan bahwa sampel mengandung antibodi anti BVD akibat pernah terinfeksi oleh virus BVD secara sementara (transient) atau melalui vaksinasi. Berdasarkan dokumen health certificate dari negara asal tidak terdapat informasi yang jelas terhadap perlakuan vaksinasi BVD pada sapi potong impor. Oleh karena itu diperlukan pemeriksaan screening di negara Indonesia untuk mendeteksi dan melakukan usaha preventif mencegah penyebaran di feedlot. Faktor-Faktor yang mempengaruhi kejadian hasil ELISA antibodi positif BVD, terkait dengan penyebaran penyakit BVD selama dalam masa pemeliharaan dan penggemukkan adalah program biosekuriti pada peternakan dengan nilai (OR=3,316; CI=1,380-7,967), dan pengelolaan limbah kandang dalam peternakan dengan nilai (OR=2,667; CI=1,105-6,434). Hasil ini menunjukkan ada asosiasi antara kedua faktor yang ada pada peternakan dengan kejadian penyakit BVD.Kata kunci: BVD, ELISA antibodi dan antigen, faktor risiko. (Detection and Risk Factors Study of Bovine Viral Diarrhea in Cattle Imports at Tanjung Priok Port)This research was a serological study on bovine viral diarrhea (BVD) and also to detect the relevance of maintenance farm management as a risk factor on the spreads of BVD. The initial screening test was performed using antibody capture enzyme linked immunosorbent assay (ELISA) to BVD on 100 cattle blood serum samples. The screening test showed that of 63 samples were positive to BVD antibody and 37 samples were negative to BVD antibody. The next screening test was performed using antigen capture ELISA to BVD and all samples showed negative results on BVD antigen. The results of the ELISA test positive for antibodies to BVD indicates that the samples examined anti-BVD antibodies due to BVD virus had been infected by a temporary (transient) or vaccination. Based on the document health certificate from the country of origin there is no clear information on the treatment of BVD vaccination on imports of beef cattle. So, we need a screening examination in the country of Indonesia to detect and perform preventive measures to prevent the spread in feedlots. Relevant factors that affected the occurrence of positive result on BVD antibody detection was farm biosecurity programs with odds ratio (OR) value of 3.316 and confidential interval (CI) value of 1.380-7.967. Further relevant factor was caging waste management with OR value of 2.667 and CI value of 1.105-6.434. There were statistically significant differences (p<0.05) between farm biosecurity programs and caging waste management related to BVD disease incidence.Keywords: BVD, ELISA antibodies and antigen, risk factors
DETEKSI MOLEKULER DAN KERAGAMAN VIRUS NEWCASTLE DISEASE PADA AYAM KAMPUNG DI WILAYAH ACEH D, Darniati; Setiyaningsih, Surachmi; Indrawati, Agustin
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (540.562 KB) | DOI: 10.21157/j.ked.hewan.v9i2.2841

Abstract

Tujuan dari penelitian ini adalah untuk mendeteksi keberadaan virus Newcastle disease (VND) dan mengkaji keragaman dari virus terisolasi. Sampel penelitian berupa usapan kloaka dan orofaring dari 177 ekor ayam kampung yang diambil dari unggas pekarangan dan pasar unggas di 12 kecamatan dalam wilayah Kabupaten Aceh Besar dan Kota Banda Aceh. Penapisan virus dilakukan pada sampel pool dengan real-time reverse transcriptation-polymerase chain reaction (rRT-PCR) dengan target gen matriks. Inokulasi 309 sampel representasi 157 ayam asal pool positif matriks pada telur ayam berembrio spesifik pathogen free (SPF) menghasilkan 69 isolat yang berasal dari 51 ekor ayam. Sebagian besar (45,09%)ayam mengeluarkan virus melalui orofaring, 25,39% melalui kloaka dan orofaring, serta 19,61% melalui kloaka. Karakterisasi keragaman isolat dilakukan dengan uji hemagglutination inhibition (HI) menggunakan serum Komarov dan Hitchner B1, rRT-PCR gen fusi dan uji elusi. Adanya keragaman epitop permukaan virus ditunjukkan dengan titer HI yang bervariasi antar isolat, perbedaan mencapai 4 log dengan serum Komarov, dan 3 log dengan B1. Sebagian besar isolat mempunyai afinitas yang lebih tinggi terhadap serum Komarov yang mengindikasikan kecenderungan kepada galur virulen. Penentuan patogenisitas menggunakan rRT-PCR menunjukkan 73,95% isolat termasuk ke dalam galur virulen (mesogenik/velogenik), sementara dari uji elusi menunjukkan 72,46% isolat termasuk galur velogenik, 20,29% mesogenik dan 7,25% dari galur lentogenik.