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Kesesuaian Hasil Pemeriksaan Antibodi Virus Herpes Simpleks Metode Enzyme-Linked Immunofiltration Assay dengan Enzyme-Linked Immunosorbent Assay

Majalah Kedokteran Bandung Vol 44, No 3 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Infeksi virus herpes simpleks (HSV) merupakan infeksi yang disebabkan oleh HSV tipe 1 (HSV-1) dan HSV tipe 2 (HSV-2). HSV-1 biasanya menyebabkan penyakit orofasial, sedangkan HSV-2 biasanya menyebabkan infeksi perigenital. Diagnosis infeksi HSV ditegakkan berdasarkan anamnesis, pemeriksaan fisis, dan laboratorium. Metode deteksi anti-HSV metode enzyme-linked immunosorbent assay (ELISA) memiliki sensitivitas 93–100% dan spesifisitas 95–100%, sedangkan metode enzyme-linked immunofiltration assay (ELIFA) memiliki sensitivitas 83,36–97% dan spesifisitas 83,93–98%. Tujuan penelitian adalah menilai kesesuaian hasil pemeriksaan anti-HSV antara metode ELIFA dan ELISA. Bila terdapat kesesuaian yang baik maka metode ELIFA dapat menggantikan metode ELISA. Penelitian dilakukan di laboratorium klinik RSUP Dr. Hasan Sadikin Bandung sejak bulan Januari–Mei 2011. Rancangan penelitian adalah potong lintang. Subjek penelitian adalah serum penderita tersangka infeksi HSV. Dilakukan analisis statistik untuk menilai agreement Kappa. Sebanyak 66 sampel diperiksa anti-HSV metode ELIFA dan ELISA. Hasil pemeriksaan IgM anti-HSV antara metode ELIFA dan ELISA memiliki kesesuaian baik (p<0,001; K=0,621), hasil pemeriksaan IgG anti-HSV-1 antara metode ELIFA dan ELISA memiliki kesesuaian sedang (p<0,001; K=0,533), dan hasil pemeriksaan IgG anti-HSV-2 antara metode ELIFA dan ELISA memiliki kesesuaian kurang (p=0,006; K=0,260). Simpulan, hanya pemeriksaan IgM anti-HSV metode ELIFA yang memiliki hasil kesesuaian baik dengan metode ELISA, sedangkan pemeriksaan IgG anti-HSV metode ELIFA memiliki kesesuaian sedang atau kurang. [MKB. 2012;44(3):152–8].Kata kunci: IgM anti-HSV, IgG anti-HSV, kesesuaian, metode ELIFA, metode ELISA Agreement of Herpes Simplex Virus Antibody Test Result between Enzymelinked Immunofiltration and Enzyme-linked Immunosorbent Assay MethodsHerpes simplex virus (HSV) infections are very common and are caused by HSV type 1 (HSV-1) and HSV type 2 (HSV-2). HSV-1 being mostly associated with orofacial disease, whereas HSV-2 is usually associated with perigenital infection. Diagnosis of HSV infection is established based on history, physical and laboratory examination. Enzyme-linked immunosorbent assay (ELISA) method to detect anti-HSV has a sensitivity 93–100% and specificity 95–100%, whereas enzyme-linked immunofiltration assay (ELIFA) has a sensitivity 83.36–97% and specificity 83.93–98%. The aim of this study was to assess the agreement of anti-HSV between ELIFA and ELISA methods. This study was conducted in the clinical laboratory RSUP Dr. Hasan Sadikin Bandung since January to May 2011. The study design was cross sectional. Subjects of this study were serum of patients suspected HSV infection. Statistical analysis was performed to assess Kappa agreement. A total of 66 samples were examined anti-HSV using ELIFA and ELISA method. There was good agreement between test results of anti-HSV IgM ELIFA and ELISA method (p<0.001, κ=0.621), moderate agreement between test results of anti- HSV-1 IgG ELIFA and ELISA method (p<0.001, κ=0.533), and fair agreement between test results of anti-HSV-2 IgG ELIFA and ELISA method (p=0.006, κ= 0.260). In conclusions, only the anti-HSV IgM ELIFA method has good agreement with ELISA. DOI: http://dx.doi.org/10.15395/mkb.v44n3.86 

Phytochemical Assay and Antiplatelet Activity of Fractions of Velvet Bean Seeds (Mucuna pruriens L.)

HAYATI Journal of Biosciences Vol 17, No 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Platelet aggregation is an important factor contributing to the formation of thrombus due to an uncontrolled blood clotting. An antiplatelet agent is a compound which decreases platelet aggregation and inhibits thrombus formation. The objectives of this study were to determine the class of compound employing phytochemical assay and to determine the in vitro antiplatelet activity of four fraction, namely hexane, ethyl acetate, butanol, and water fractions of velvet bean seeds (Mucuna pruriens L.) using epinephrine (EPN) as agonist of platelet aggregation. The antiplatelet activities were tested in human platelet rich plasma with hyperaggregation. To determine the activities, EPN was arranged at 4 level of concentrations  (300, 150, 75, and 30 μM), and antiplatelet agents were at 500 µg/ml. The results indicated that ethyl acetate, butanol and water fraction contained high flavonoids and moderate phenols. The water, butanol and ethyl acetate fractions of velvet bean seeds exhibited potential inhibition of EPN-induced platelet aggregation at all concentrations. The strongest antiplatelet agent was water fraction and had the same antiplatelet activity as aspirin at level 150, 75, and 30 μM of EPN. Butanol fraction had the same antiplatelet activity as aspirin at the lowest EPN (30 μM).

POTENCY OF ANTIOXIDANT, ANTICHOLESTEROL AND PLATELET ANTIAGGREGATION OF BLACK TEA (Camelia sinensis )

Buletin Penelitian Tanaman Rempah dan Obat Vol 22, No 1 (2011): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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Abstract

Epidemiological studies have shown an in-verse correlation betweendiets rich in po-lyphenols, flavonoids and reduced risk of cardiovasculardisease. These associationswere mainly ascribed to the antioxidant, antiaggregation and anticholesterol capa-city of polyphenols. This association has been explained that atherogenesis is ini-tiated by hypercholesterol, lipid peroxida-tion and hyper aggregation platelet. The research was carried out to evaluate anti-oxidant, anticholesterol and antiaggrega-tion activities of methanol extract and frac-tions of black tea (Camelia sinensis). To evaluate antioxidant activity of methanol extract and fractions were compared  with (-)-Epigallocatechine 3-gallate (EGCG), an-ticholesterol activity were compared with simvastatin and antiaggregation activity were compared with aspirin. Antioxidant activity using the 1,1-diphenyl-2-picryl-hy-drazyl (DPPH) free radical scavenging acti-vity at two concentrations 100 µg/mL, 50 µg/mL and anticholesterol was cholesterol oxidase activity at two concentrations 0.005 µg/mL, 0.0025 µg/mL, the antiag-gregation  activity  used epinephrine (EPN)agonist at two concentrations 300 μM, 75 μM and one concentration 37.1 µg/mL of methanol extract and fractions of black tea. To know the difference of treatment, the data were analysed using Analysis of Variance (ANOVA) and be continued Dun-can’s new multiple range test. The results showed that DPPH scavenging activity were high (88.59-93.556%), the anticho-lesterol showed high activity (93.663-97.434%) and the antiaggregation sho-wed low up to high activity (79.967-4.31%). Using Duncan’s new multiple ra-nge test showed that anti-oxidant activity were not different among extract and fractions of black tea,the highest anti-cholesterol activity was black tea extract 0.005 µg/mL (97.434%) and the highest antiaggregation activity was ethyl acetate fraction on 75 μM EPN agonist (4.310 %), water fraction 37.1 µg/mL with 300 μM EPN agonist (21.833%).