Iman Rusmana
Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University. Jl. Raya Darmaga, Bogor 16680, West Java. Indonesia

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Journal : BIOTROPIA

ISOLATION AND CHARACTERIZATION OF METHANOTROPHIC BACTERIA FROM RICE FIELDS Iman Rusmana; ALINA AKHADIYA
BIOTROPIA Vol 16, No 2 (2009)
Publisher : SEAMEO BIOTROP

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Abstract

Methane is a greenhouse gas and has potency to deplete ozone layer.  Rice fields are a significantly sources of atmospheric methane.  Chemical fertilizers application in rice fields can increase methane emission. Methanotrophic bacteria are unique in their ability to utilize methane as a sole carbon and energy source.  This research was successfully isolating and characterizing methanotrophic bacteria from rice fields in Bogor and Sukabumi, West Java, Indonesia.  Determination of methane oxidation activity using Gas Chromatography showed that all isolates performed methane oxidation activity.  The highest methane oxidation activity was performed by BGM 9 isolate. And DNA amplification of BGM 9 genome was performed single band of mmoX in the size of 500 bp and three bands of pmoA  in the size of 1000, 750 and 500 bp respectively
Co-Authors , Lizawati , Sobir -, Rahman . Mahyarudin, . Achmad Dinoto Aliati Iswantari ALINA AKHADIYA ALINA AKHDIYA Amanda, Nisa Widya Ani Suryani ANIK KUSMIATUN ANIK KUSMIATUN ANJA MERYANDINI Antonius - Suwanto ANTONIUS SUWANTO ANTONIUS SUWANTO Ari Fina Bintarti, Ari Fina ARIS TRI WAHYUDI Baskoro S Wibowo, Baskoro S Cahyadi Irwan, Cahyadi Cahyadi, Alfan CECEP KUSMANA CECILIA ANNA SEUMAHU Daniel Djokosetiyanto DEDY DURYADI SOLIHIN Desniar - - Desniar -, Desniar Desniar . DIAH ISKANDRIATI DIAH ISKANDRIATI DONDIN SAJUTHI DONDIN SAJUTHI DWI NINGSIH SUSILOWATI Eddy Supriyono Fachriyan Hasmi Pasaribu Ferbiyanto, Andri Fredinan Yulianda HAPPY WIDIASTUTI Hefni Effendi I MADE ARTIKA I Made Samudra It Jamilah KATHARINA JESSICA KATHARINA JESSICA Khairul Syahputra, Khairul Komang Gede Wiryawan Kukuh Nirmala Kuntari, Wahyu Budi Lena Novita MIFTAHUDIN ., MIFTAHUDIN Munti Yuhana MUTTAQIN, MAFRIKHUL Niken T.M Pratiwi, Niken T.M NISA RACHMANIA MUBARIK Novita, Hessy PANJI, TRI - PRIHASTO SETYANTO Puspaningsih, Dewi Rahman Rahman RATNA SETYANINGSIH RATNANINGTYAS, SUSI Ridwan Affandi RIKA RAFFIUDIN Rina Hesti Utami ROEDHY POERWANTO Siti Nur Jannah SRI BUDIARTI SRI BUDIARTI SUSAN SOKA SUSAN SOKA T. Widiyanto Thenawidjaya, Maggy TRI EKO WAHYONO, TRI EKO Tri Handayani Kurniati Tri Muji Ermayanti Tri Puji Priyatno Tri Widiyanto UTUT WIDYASTUTI Wibowo, Risky Hadi Widiyati, Ani Yadi Suryadi Yohana A Dahliani, Yohana A YULIN LESTARI Yuni Puji Hastuti Yuni Puji Hastuti Yusli Wardiatno Yusup, Ciptadi Achmad