Iman Rusmana
Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University. Jl. Raya Darmaga, Bogor 16680, West Java. Indonesia

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Journal : MEDIA PETERNAKAN - Journal of Animal Science and Technology

Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics Jannah, Siti Nur; Dinoto, Achmad; Wiryawan, Komang Gede; Rusmana, Iman
Media Peternakan - Journal of Animal Science and Technology Vol 37, No 3 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (771.819 KB) | DOI: 10.5398/medpet.v37i3.7969

Abstract

The aims of this study were to screen and characterize lactic acid bacteria (LAB) isolated from gastrointestinal (GI) tract of Cemani chicken, one of Indonesian local chicken and to investigate their potential use as probiotics. LAB were isolated from GI tract using MRSA and GYPA media and incubated anaerobically. Selected LAB were determined their probiotic properties with several assays. Identification of selected LAB was based on 16S rDNA sequences, morphological and biochemical characteristics. Ninety five bacteria  were isolated and characterized as lactic acid bacteria (Gram positive, catalase negative, non sporeforming and acid producing). Twenty four isolates of LAB demonstrated antimicrobial activity against Escherichia coli JCM 1649 and Salmonella enteritidis B2586, and three selected isolates, i.e. CCM011, CSP004, and CVM002 showed the highest inhibition activity. The isolates had characters of high cell surface hydrophobicity and inter-isolate coaggregation ability of LAB, high survival at low pH, high  phytase and protease activity (but no amylase and lipase activity), weak coaggregation with pathogen and no resistance to the examined antibiotics. The isolates were identified based on sequence analysis of 16S rRNA gene as Lactobacillus salivarius, however, each isolate had different profiles of sugar fermentation. Therefore the three LAB isolates had potential application as probiotics for chicken. Key words: Cemani chicken, gastrointestinal tract, lactic acid bacteria, probiotic
Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics Jannah, Siti Nur; Dinoto, Achmad; Wiryawan, Komang Gede; Rusmana, Iman
Media Peternakan Vol 37, No 3 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (771.819 KB) | DOI: 10.5398/medpet.2014.37.3.182

Abstract

The aims of this study were to screen and characterize lactic acid bacteria (LAB) isolated from gastrointestinal (GI) tract of Cemani chicken, one of Indonesian local chicken and to investigate their potential use as probiotics. LAB were isolated from GI tract using MRSA and GYPA media and incubated anaerobically. Selected LAB were determined their probiotic properties with several assays. Identification of selected LAB was based on 16S rDNA sequences, morphological and biochemical characteristics. Ninety five bacteria  were isolated and characterized as lactic acid bacteria (Gram positive, catalase negative, non sporeforming and acid producing). Twenty four isolates of LAB demonstrated antimicrobial activity against Escherichia coli JCM 1649 and Salmonella enteritidis B2586, and three selected isolates, i.e. CCM011, CSP004, and CVM002 showed the highest inhibition activity. The isolates had characters of high cell surface hydrophobicity and inter-isolate coaggregation ability of LAB, high survival at low pH, high  phytase and protease activity (but no amylase and lipase activity), weak coaggregation with pathogen and no resistance to the examined antibiotics. The isolates were identified based on sequence analysis of 16S rRNA gene as Lactobacillus salivarius, however, each isolate had different profiles of sugar fermentation. Therefore the three LAB isolates had potential application as probiotics for chicken.Key words: Cemani chicken, gastrointestinal tract, lactic acid bacteria, probiotic
Co-Authors , Lizawati , Sobir -, Rahman . Mahyarudin, . Achmad Dinoto Aliati Iswantari ALINA AKHADIYA ALINA AKHDIYA Amanda, Nisa Widya Ani Suryani ANIK KUSMIATUN ANIK KUSMIATUN ANJA MERYANDINI Antonius - Suwanto ANTONIUS SUWANTO ANTONIUS SUWANTO Ari Fina Bintarti, Ari Fina ARIS TRI WAHYUDI Baskoro S Wibowo, Baskoro S Cahyadi Irwan, Cahyadi Cahyadi, Alfan CECEP KUSMANA CECILIA ANNA SEUMAHU Daniel Djokosetiyanto DEDY DURYADI SOLIHIN Desniar - - Desniar -, Desniar Desniar . DIAH ISKANDRIATI DIAH ISKANDRIATI DONDIN SAJUTHI DONDIN SAJUTHI DWI NINGSIH SUSILOWATI Eddy Supriyono Fachriyan Hasmi Pasaribu Ferbiyanto, Andri Fredinan Yulianda HAPPY WIDIASTUTI Hefni Effendi I MADE ARTIKA I Made Samudra It Jamilah KATHARINA JESSICA KATHARINA JESSICA Khairul Syahputra, Khairul Komang Gede Wiryawan Kukuh Nirmala Kuntari, Wahyu Budi Lena Novita MIFTAHUDIN ., MIFTAHUDIN Munti Yuhana MUTTAQIN, MAFRIKHUL Niken T.M Pratiwi, Niken T.M NISA RACHMANIA MUBARIK Novita, Hessy PANJI, TRI - PRIHASTO SETYANTO Puspaningsih, Dewi Rahman Rahman RATNA SETYANINGSIH RATNANINGTYAS, SUSI Ridwan Affandi RIKA RAFFIUDIN Rina Hesti Utami ROEDHY POERWANTO Siti Nur Jannah SRI BUDIARTI SRI BUDIARTI SUSAN SOKA SUSAN SOKA T. Widiyanto Thenawidjaya, Maggy TRI EKO WAHYONO, TRI EKO Tri Handayani Kurniati Tri Muji Ermayanti Tri Puji Priyatno Tri Widiyanto UTUT WIDYASTUTI Wibowo, Risky Hadi Widiyati, Ani Yadi Suryadi Yohana A Dahliani, Yohana A YULIN LESTARI Yuni Puji Hastuti Yuni Puji Hastuti Yusli Wardiatno Yusup, Ciptadi Achmad