Iman Rusmana
Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University. Jl. Raya Darmaga, Bogor 16680, West Java. Indonesia

Published : 47 Documents
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Journal : Biosaintifika: Journal of Biology

The Effect of Biofertilizer on The Diversity of N2O Reducing Bacteria in Paddy Fields of Sukabumi, Indonesia Cahyadi, Alfan; Rusmana, Iman; Mubarik, Nisa Rachmania
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.9796

Abstract

Some of the methanotrophic bacteria and N2O reducing bacteria have been proven to be able to support the plant growth and increase the productivity of paddy. However effect of the methanotrophic and N2O reducing bacteria application as a biofertilizer to indigenous N2O reducing bacteria is still not well known yet. The aim of this study was to analyze the diversity of N2O reducing bacteria in lowland paddy soil based on a nosZ gene. Soil samples were taken from lowland paddy soils in Pelabuhan Ratu Sukabumi, West Java, Indonesia. There were two treatments for the paddy field soil, ie. biofertilizer-treated field 20% fertilizer (50 kg/ha) with the addition of biofertilizer and 100% fertilizer. PCR amplification of nosZ gene was successfully conducted using nosZF and nosZR primer pair. Denaturing Gradient Gel Electrophoresis (DGGE) process was conducted at 150 V for 5.5h. There were three differences nosZ bands were sequenced. The phylogenetic analysis showed that they were close to uncultured bacteria. Microbial diversity in the biofertilizer-treated field was higher than that of in the 100% fertilizer-treated field. The biofertilizer treatment has higher in microbial diversity than that of applied non-biofertilizer paddy fields. This research might have impact in the application of biofertilizers due to the emission of N2O as a green house gas from paddy fields farming activity. The biofertilizer has great potential application in sustainable environmental friendly agriculture systems.
Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10 Kurniati, Tri Handayani; Rusmana, Iman; Suryani, Ani; Mubarik, Nisa Rachmania
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6448

Abstract

Pyrene degradation and biosurfactant activity by a new strain identified as Gordonia cholesterolivorans AMP 10 were studied. The strain grew well and produced effective biosurfactants in the presence of glucose, sucrose, and crude oil. The biosurfactants production was detected by the decreased surface tension of the medium and emulsification activity. Analysis of microbial growth parameters showed that AMP10 grew best at 50gmL-1pyrene concentration, leading to 96% degradation of pyrene within 7days. The result of nested PCR analysis revealed that this isolate possessed the nahAc gene which encodes dioxygenase enzyme for initial degradation of Polycyclic Aromatic Hydrocarbon (PAH). Observation of both tensio-active and emulsifying activities indicated that biosurfactants which produced by AMP 10 when grown on glucose could lower the surface tension of medium from 71.3 mN/m to 24.7 mN/m and formed a stable emulsion in used lubricant oil with an emulsificationindex (E24) of 74%. According to the results, it is suggested that the bacterial isolates G. cholesterolivorans AMP10 are suitable candidates for bioremediation of PAH-contaminated environments.How to CiteKurniati, T. H., Rusmana, I. Suryani, A. & Mubarik, N. R. (2016). Degradation of Polycyclic Aromatic Hydrocarbon Pyrene by Biosurfactant-Producing Bacteria Gordonia cholesterolivorans AMP 10. Biosaintifika: Journal of Biology & Biology Education, 8(3), 336-343.
Co-Authors , Lizawati , Sobir -, Rahman . Mahyarudin, . Achmad Dinoto Aliati Iswantari ALINA AKHADIYA ALINA AKHDIYA Amanda, Nisa Widya ANGELA MARIANA LUSIASTUTI Ani Suryani ANIK KUSMIATUN ANIK KUSMIATUN ANJA MERYANDINI Antonius - Suwanto ANTONIUS SUWANTO ANTONIUS SUWANTO Ari Fina Bintarti, Ari Fina ARIS TRI WAHYUDI Baskoro S Wibowo, Baskoro S Cahyadi Irwan, Cahyadi Cahyadi, Alfan CECEP KUSMANA CECILIA ANNA SEUMAHU Daniel Djokosetiyanto DEDY DURYADI SOLIHIN Desniar - - Desniar -, Desniar Desniar . DIAH ISKANDRIATI DIAH ISKANDRIATI DONDIN SAJUTHI DONDIN SAJUTHI DWI NINGSIH SUSILOWATI Eddy Supriyono Fachriyan Hasmi Pasaribu Ferbiyanto, Andri Fredinan Yulianda HAPPY WIDIASTUTI Hefni Effendi I MADE ARTIKA I Made Samudra It Jamilah KATHARINA JESSICA KATHARINA JESSICA Khairul Syahputra, Khairul Komang Gede Wiryawan Kukuh Nirmala Kuntari, Wahyu Budi Lena Novita MIFTAHUDIN ., MIFTAHUDIN Munti Yuhana MUTTAQIN, MAFRIKHUL Niken T.M Pratiwi, Niken T.M NISA RACHMANIA MUBARIK Novita, Hessy Novita, Hessy PANJI, TRI - PRIHASTO SETYANTO Puspaningsih, Dewi Rahman Rahman RATNA SETYANINGSIH RATNANINGTYAS, SUSI Ridwan Affandi RIKA RAFFIUDIN Rina Hesti Utami ROEDHY POERWANTO Siti Nur Jannah SRI BUDIARTI SRI BUDIARTI SUSAN SOKA SUSAN SOKA T. Widiyanto Thenawidjaya, Maggy TRI EKO WAHYONO, TRI EKO Tri Handayani Kurniati Tri Muji Ermayanti Tri Puji Priyatno Tri Widiyanto UTUT WIDYASTUTI Wibowo, Risky Hadi Widiyati, Ani Yadi Suryadi Yohana A Dahliani, Yohana A YULIN LESTARI Yuni Puji Hastuti Yuni Puji Hastuti Yusli Wardiatno Yusup, Ciptadi Achmad