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Combination of Testosterone Undecanoate (TLI) and Water Extract of Medan Blustru (Lulfa aegtptiaca Milt.) as Alternatif in Male Contraceptive Ilyas, Syafruddin
Journal of the Indonesian Medical Association Vol. 61 No. 10 October 2011
Publisher : Journal of the Indonesian Medical Association

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Abstract

Combination of Testosterone Undecanoate 8U) and Blustru Medan (Luffa aeg,ptincaMilL) was administrated to male mouse (Mus musculus L.). The aim this research is to evaluatethe correlation ofplasm testosterone with quality and quantigt of sperm. Male mouse was injected with 0,25 mg of TU/6 weeles and 270 mg/25 g body weight of Blustru Medan/day. Condition of these treatments were recorded at 0 (0week), 6 (6hweek), 12 (12h week) and 18 (1&h week). Each treatment had its control. The serum testosterone, quality (morphologt andviability) and quantity (concentration) of sperm were analized after administration combination of TU and Blustru Medan. Result of this research was significant dffirence at the plasm testosterone, quality and quantity of sperm at the group of I8h week treatment (p<0,05). There were also strong and significant corelation between viability vs concentration of sperm (0,928; p<0,001). In conclusion W+Blustru Medan was able to decrease spefln concentration and also decreasing serum testosterone, morpholog,t and viability of sperm. J Indon Med Assoc 2011;61:405-11.Keywords: Testosterone Undecanoate, Blustru Medan (Lttffa aegyptiaca Mill.), sperm normalmorfo log, sperm viability, sperm conc entration
Pengaruh Media Tanam dan Pupuk Daun terhadap Aklimatisasi Anggrek Dendrobium (Dendrobium sp) Wardani, Sri; Setiado, Hot; Ilyas, Syafruddin
Jurnal Ilmu Pertanian KULTIVAR Vol 5, No 1 (2011)
Publisher : Jurnal Ilmu Pertanian KULTIVAR

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Penelitian ini bertujuan untuk mengetahui pengaruh jenis media tumbuh aklimatisasi dan jenis pupuk daun terhadap pertumbuhan pada proses aklimitasiasi anggrek Dendrobium sp. Penelitian ini dilaksanakan di Laboratorium Pemuliaan Tanaman, Fakultas Pertanian, Universitas Sumatera Utara, Medan yang dimulai dari bulan Januari sampai dengan Maret 2009. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan dua faktor. Faktor pertama adalah jenis media tumbuh aklimatisasi (arang sekam, cocopeat, dan campuran antara arang sekam dan cocopeat). Faktor yang kedua adalah jenis pupuk daun (pupuk majemuk foliar I dan pupuk majemuk foliar II). Parameter yang diamati adalah persentase hidup, pertambahan tinggi planlet, pertambahan diameter batang, pertambahan jumlah daun, pertambahan berat total, saat muncul tunas, jumlah tunas, berat akar, volume akar dan jumlah klorofil daun. Hasil penelitian menunjukkan bahwa interaksi jenis media tumbuh aklimatisasi dengan jenis pupuk daun berpengaruh nyata terhadap parameter saat muncul tunas dan jumlah tunas. Kata kunci: acclimitazation, orchid, Dendrobium sp
GAMBARAN HISTOLOGIS HEPAR MENCIT (Mus musculus L.) STRAIN DDW SETELAH PEMBERIAN EKSTRAK N-HEKSAN BUAH ANDALIMAN (Zanthoxylum acanthopodium DC.) SELAMA MASA PRA IMPLANTASI DAN PASCA IMPLANTASI Prasetiawan, Eka; Sabri, Emita; Ilyas, Syafruddin
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

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Liver histological features of DDW mice (Mus musculus L.) treated with n-hexane extract of Andaliman (Zanthoxylum acanthopodium DC.) fruit during pre- and post-implantation has been studied in Laboratory of Animal Structure, Department of Biology, Faculty of Mathematics and Natural Science, University of Sumatera Utara, Medan. Experiment used completely randomized design (CRD) consisted of 2 periods of treatment: pre-implantation (day 0-3; group A) and post-implantation (day 6-14; group B). For each group,  mice were treated daily by oral gavage with 0, 1, 2, 4, or 6 % of andaliman extract in 0.3 ml of 1% CMC. The result showed, liver in treatment groups was pale and showed mottled surface texture. Hepatocyte damage in post-implantation group was higher than in pre implantation group (p> 0.05). We concluded that n-hexane extract of andaliman fruit appears to negatively affect liver histology of mice. Keywords: liver histology, andaliman (Zanthoxylum acanthopodium DC.),  post implantation, pre implantation
GAMBARAN HISTOLOGIS PULMO MENCIT JANTAN (Mus musculus L.) SETELAH DIPAPARI ASAP ROKOK ELEKTRIK Triana, Nanin; Ilyas, Syafruddin; Hutahaean, Salomo
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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The effects of electric cigarette exposure on lung tissue of mice were studied. The experiments using completely randomized design (CRD) with 3 treatments, i.e.  treatment of electric cigarette with strawberry flavour, tobacco flavour, and control treatment that was not exposed by cigarette smoke. Eight male mice were used for each group. Exposure treatment was done every day for 2 consecutive weeks with dosage 20 times suction using syringe of 60 mL. After receiving treatment for 2 weeks, the mice were killed by cervical dislocation. The lungs were isolated and prepared histologically using paraffin method and then stained with by haematoxylin and eosin  (HE). The results showed that, smoke treatment mice lose weight significantly (p< 0.05). In gross anatomy, electric cigarrete smoke affect lung feature, but histological observation on  membrane damage, damage to the lumen and the relationship between alveoli the smoke effect were statistically unsignificant (p>0.05).. In summary short period exposure to electric  cigarette smoke  tends affect lung damage in anatomy, but statistically has no effect of the damage to the lung tissue.   Keywords: alveolar lumen, alveolar membrane, electric cigarettes
PENGARUH PEMBERIAN VITAMIN C DAN E TERHADAP GAMBARAN HISTOLOGIS HEPAR MENCIT (Mus musculus L.) YANG DIPAJANKAN MONOSODIUM GLUTAMAT (MSG) Maulida, Ahri; Ilyas, Syafruddin; Hutahaean, Salomo
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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This study aimed to determine the effects of vitamin C and E on liver microstructure of mouse exposed to monosodium glutamate (MSG) for 30 days. The experiment used completely randomized design which consists of 6 treatments and  5 replications. Mice were treated daily by oral gavage with 4 mg MSG (P1),  4 mg MSG + 0.26 mg  vitamin C (P2),  4 mg MSG + 0.026 vitamin E (P3), or  4 mg  MSG + 0.26 mg  vitamin C + 0.026 mg vitamin E (P4). All dosages were based on mg/g body weight. Two control groups K- and K+ were treated with water and castor oil respectively. The mice liver tissue slide was prepared by the paraffin method and stained with haematoxylin-eosin (HE). The results showed that MSG caused cell degeneration and necrosis in liver tissue. Vitamin C and E was  able to recover  liver cell damage induced by MSG.  The highest result was found in P3 and combination treatment (P4) where the recovery effect reaches 64% and 80% respectively. Keywords : Liver, Monosodium glutamate (MSG), Vitamin C, Vitamin E.
PENGARUH PEMBERIAN VITAMIN C DAN E TERHADAP GAMBARAN HISTOLOGIS GINJAL MENCIT (Mus musculus L.) YANG DIPAJANKAN MONOSODIUM GLUTAMAT (MSG) Zulfi, Zulfiani; Ilyas, Syafruddin; Hutahaean, Salomo
Saintia Biologi Vol 1, No 3 (2013)
Publisher : Saintia Biologi

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The aim of this research is to study the effect of vitamin C and E on kidney microstructure of mouse exposed to monosodium glutamate for 30 days. This research used complete randomized design which consisted of 6 treatments and 5 replications. The control consisted of  K-, and K+ (castor oil 0.3 ml). The treatments were MSG 4 mg/g BW, P1 (only MSG), P2 (MSG plus vitamin C 0.26 mg/g BW), P3 (MSG plus vitamin E 0.026 mg/g BW) and P4 (MSG plus vitamin C and E). After treatments were completed, the mice were sacrificed by cervical dislocation, and kidney tissue section was prepared by  the paraffin method and  stained with Haematoxylin and Eosin staining. Microstructure damage of kidneys showed a significant difference between control and treatments. In P1, the proximal tubule damage was 69.95%±5 and in P4 was 47.22%±3. In summary MSG can cause damage in the form of a narrowing of the lumen of the proximal tubules of the kidney. A combination of vitamins C and E can reduce the effect of MSG on the kidney.   Keywords: kidney, Monosodium glutamat (MSG), Vit C, Vit E.
PENGARUH PEMBERIAN VITAMIN C DAN E TERHADAP GAMBARAN HISTOLOGIS TESTIS MENCIT (Mus musculus L.) YANG DIPAJANKAN MONOSODIUM GLUTAMAT (MSG) Kalsum, Ummi; Ilyas, Syafruddin; Hutahaean, Salomo
Saintia Biologi Vol 1, No 3 (2013)
Publisher : Saintia Biologi

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The aim of this study is to determine the effect of vitamin C and E on  testicular histology of mice following exposure to monosodium glutamate (MSG) for 30 days. Completely Randomized Design (CRD) consisting of 6 treatments and 5 replications was used in this experiment. Mice were treated daily by oral gavage with 4 mg MSG (P1),  4 mg MSG + 0,26 mg  vitamin C (P2),  4 mg MSG + 0,026 vitamin E (P3), and  4 mg  MSG + 0,26 mg  vitamin C + 0,026 mg vitamin E (P4). All dosages (mg/g) were given based on body weight. Two control groups K- and K+ treated with water and castor oil respectively. The results showed that MSG decrease testis weight and its volume (P<0,05), MSG also decrease the diameter of seminiferous tubule and the number of spermatogenic cells. Vitamin C recovers testis weight (P<0,05), but cannot recover its volume, diameter of seminiferous tubules and the number  spermatogenic cells (P>0,05). Vitamin E increases the weight and volume of the testes and spermatogenic cells (P<0,05), but unable to recover diameter of the seminiferous tubules (P>0,05). The combination of vitamin C and E recover testis weight and its volume, seminiferous  tubule  diameter  and number  of  spermatogenic  cells (P<0,05). Keywords: Monosodium Glutamate, Vitamin C, Vitamin E, Testis
PENGARUH RADIASI SINAR GAMMA TERHADAP TANAMAN KACANG HIJAU (Vigna radiata L.) PADA KONDISI KEKERINGAN Sianipar, Jelita; Putri, Lollie Agustina.P; Ilyas, Syafruddin
AGROEKOTEKNOLOGI Vol 1, No 2
Publisher : Program studi Agroekoteknologi, Fakultas Pertanian, Universitas Sumatera Utara-Medan

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Demand of mungbean will be increase, so the production must be increased. One of efforts is to create superior variety by giving mutation.The goal of this research is to find out the effect of  four level radiation dosage and drought stress to growth and yield of  mungbean (Vigna radiata L.), has finished in greenhouse of Agriculture Faculty of North Sumatera University, Medan conducted from May until August 2012. This Research was conducted using by Randomize Block Design factorials.The first factor is radiation dosage with four levels : 0, 10, 20, and 30 krad. The second factors is drought stress:  100%, 80%, 60%,and 40% KL. Data were analyzed with ANOVA and continued with HSD.The result of research showed the radiation significantly affected to the harvesting time. Drought stress significantly affected to the root volume, the number of pod per plant,and weight seeds per plant. The combination between dose of radiation and drought stress significantly affected to flowering time.   Key word : mungbean, radiation, drought stress
PENGUJIAN RESPONS PERTUMBUHAN BEBERAPA VARIETAS PADI SAWAH (Oryza sativa L.) AKIBAT CEKAMAN SALINITAS Hutajulu, Herti Folarida; Rosmayati, Rosmayati; Ilyas, Syafruddin
AGROEKOTEKNOLOGI Vol 1, No 4 (2013)
Publisher : Program studi Agroekoteknologi, Fakultas Pertanian, Universitas Sumatera Utara-Medan

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Growth Response Testing of Several Rice Varieties (Oryza sativa L.) Caused of Salinity. In theSub-District Percut Sei Tuan there are around 700 hectares more area paddy fields that can becategorized as has been affected by sea water (is 1-2 km to coast). The production rice fields in thearea was around 2-3 tons per hectare with using varieties Ciherang and Mekongga. But varietiesCiherang have potential result8.5 tonnes/ha with result average 6.0 tonnes/ha in the non- salinity.The problem is to be a basic idea how to increase production the be examined back some varietiesthat had been found varieties that not only could but critical market result in standard national resultrice that is 6.0 ton/ha.This study aims to determine the growth response of some rice varieties due tosalinity stress. The study was conducted in the Village of Tanjung Rejo, Hamlet Paluh Merbau, Sub-District Percut Sei Tuan, Deli Serdang Regency with altitude of 1.5 meters above sea level in April-September 2012 using a Randomized Block Design with eight varieties of rice plant (Ciherang, IR64 , Lambur, Batanghari, Banyuasin, IR 42, Inpari 10 and Margasari) with 3 replication. Parameter isobserved germination, salt injury, plant height, number of tillers, leaf chlorophyll, leaf area, numberof productive tillers, panicle length, number of grains contains per panicle, number of grains perpanicle empty, grain weight per plot, and 1000 grain weight. The results showed that Ciherangvarieties and Banyuasin is highly tolerant to salinity. Banyuasin and Batanghari production per plothad the highest and lowest are IR 64 varieties and Inpari 10 varieties.Keywords: salinity, varieties, paddy.
PENGARUH PERTUMBUHAN VEGETATIF BEBERAPA VARIETAS KEDELAI HITAM DENGAN PEMBERIAN VERMIKOMPOS PADA TANAH MASAM Nasution, Chandri Lidya Putri; Siregar, Luthfi Aziz Mahmud; Ilyas, Syafruddin
AGROEKOTEKNOLOGI Vol 2, No 1 (2013)
Publisher : Program studi Agroekoteknologi, Fakultas Pertanian, Universitas Sumatera Utara-Medan

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ABSTRACTThe effect of Vegetative Growth on some Varieties of Black Soybeans with The Provision ofVermicompost on Acid Soils.Insufficient Black soybeans productiondomestic demand is a problemthat faced today. The addition of organic matter and using acid soils is one way that can be done toincrease the production of black soybean.The objective of the research was to know the effect ofvegetativegrowth on some varieties of black soybeans (Glycine max (L.) Merrill) with the provisionof vermicompost on acid soils. The experiment was conducted in the Public Land, Pasar I, TanjungSari, Medan, with altitude ± 25 meters above sea level from July to October 2012.Using a factorialrandomized block design with the first factor application of vermicompost (0, 0.8, 1.6 and 2.4kg/m²) and the second varieties (Detam-1, Detam-2, Cikuray, Merapi, Malikka) with threereplications. The result showed that the application of vermicompost insignificanly affected to all ofparametric in the research. The variety significantly affected the plant height at 2, 3, and 5 MST,andthe number of branches on the main stem. The combine between application of vermicompost andsoybean varieties were insignificanly to all of parametric in the research.Key word : soybean, acid soils, vermicompost, varieties