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SENYAWA ASAM 2- METILESTER-1-H-PIROL-4-KARBOKSILAT DALAM EKSTRAK ETIL ASETAT BUAH SALAK VARIETAS BONGKOK SEBAGAI ANTIOKSIDAN DAN ANTIHYPERURICEMIA Afrianti, Leni Herliani; Sukandar, Elin Yulinah; Ibrahim, Slamet; Adnyana, I Ketut
Jurnal Teknologi Dan Industri Pangan Vol 21, No 1 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (371.817 KB) | DOI: 10.6066/2464

Abstract

SENYAWA ASAM 2- METILESTER-1-H-PIROL-4-KARBOKSILAT DALAM EKSTRAK ETIL ASETAT BUAH SALAK VARIETAS BONGKOK SEBAGAI ANTIOKSIDAN DAN ANTIHYPERURICEMIA [Studies on 2-Methylester-1-H-Pyrolle-4-Carboxylic Acid Compound in Ethylacetate Extract of Snake Fruit Variety Bongkok as Antioxidant and Anthyperuricemic] Leni Herliani Afrianti 1)*, Elin Yulinah Sukandar 2), Slamet Ibrahim 3), dan I Ketut Adnyana 2). 1) Departemen Teknologi Pangan Universitas Pasundan, Bandung. 2) Farmakologi-Farmasi Klinik, Sekolah Farmasi Institut Teknologi Bandung. 3) Farmasi kimia Research Group, Sekolah Farmasi Institut Teknologi Bandung.   Diterima  1 Juli 2009 / Disetujui 20 Mei  2010 ABSTRACT   The aim of the study was to determine the antioxidant and antihyperuricemia activity of ethyl acetate extract of snake fruit (Salacca edulis Reinw.) var. Bongkok. The research methods used in this study comprised  of three stages. First stage, the isolation processes, consist ed of maceration, fractionation, and purification using several techniques of chromatography. The chemical structures of the isolated compounds were determined based on UV, IR, 1-D NMR, and 2-D NMR spectral data. The ethyl acetate extract of snake fruit var. Bongkok isolated was a new compound 2-methylester-1-H-pyrolle-4- carboxylic acid. In the second stage the antioxidant activity of the extract and the isolated compounds were measured by 1,1 diphenol  (DPPH) method. The antioxidant activity of the extracts and the isolated compounds were expressed as IC50, The ethyl acetate extracts at concentrations of 0.2, 2, 20, 200, 400, and 2000 µg/mL showed inhibition of 9.67, 4.47, 41.89, 96.06, 82.54, and 90.60 % respectively, with an IC50 of 1.6 µg/mL. Ascorbic acid standards at the same concentration range showed an  IC50 of 0.54 µg/mL. Meanwhile, at the same concentrations the 2-methylester-1-H-pyrolle-4-carboxylic acid showed free radical inhibition of 17.48, 21.48, 18.14, 31.87, and 62.34 % respectively, with an  IC50 of 3.27  µg/mL. During the third stage,  the antihyperuricemic properties of the extracts and the isolated compound were examinated in vitro using inhibition of xanthin oxidase method. The ethyl acetate extracts at concentrations of 0.01, 0.02, 0.2, 2, and 2000  µg/mL showed xanthin oxidase inhibition of 49.24, 49.58, 50.28 and 52.26 % respectively, with an  IC50 of 24.75 µg/mL. At the same concentrations, the 2-methylester-1-H-pyrolle-4- carboxylic acid, showed xanthin oxidase inhibition of 27.7, 30.5, 37.3, 50.27 and 50.55 % respectively, with an IC50 of 48.86 µg/mL.  Allopurinol as a standard drug showed an IC50 of 0.92 µg/mL.   Key word : 2-methylester-1-H-pyrolle-4- carboxylic acid compound, ethyl acetate extract of snake fruit var. Bongkok, antioxidant activity, antihyperuricemia, IC50.
Determination of Iodate and Iodide Content in Iodized Salt By Ion Pair High Performance Liquid Chromatography Method Cahyadi, Wisnu; Firman, Kurnia; Ibrahim, Slamet; Kartadarma, Embit
Jurnal Teknologi Dan Industri Pangan Vol 15, No 1 (2004): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/528

Abstract

Two species of iodine, i.e. iodide and iodate in commercial iodized salt were determined using ion pair HPLC. From 15 samples analysed, the iodide and iodate content ranged from 24,05 ± 2,51 to 70,25 ± 3,78 ppm and from 31,43 ± 8,10 to 87,59 ± 0,44 ppm, respectively. The method used was found satisfactory in terms of precission, accuracy, sensitivity and selectivity, therefore the method seem acceptable for the determination of iodide and iodate content in iodized salt samples. Key words : Iodine species, determination of iodine species content, and ion-pair HPLC
Pengaruh Interaksi Fisika Amoksisilina Trihidrat-Kalium Klavulanat terhadap Peningkatan Potensi Antibiotika pada Bakteri Non-Beta Laktamase Sarcina Lutea Sp. Nugrahani, Ilma; Asyarie, Sukmadjaja; Nurono S., Sundani; Ibrahim, Slamet
Jurnal Kedokteran Maranatha Vol 7, No 2 (2008)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The relation between physical interaction amoxicillin-clavulanate with their increasing antibiotic sensitivity to non-betalactam bacteria: Sarcina lutea sp has been studied.  Amoxicillin and clavulanate were mixing as physical mixture (AC-PM) and freeze dried binary system (AC-FD). The experiment measuring the inhibition diameter to Sarcina lutea sp from physical mixture (AK-CF) and freeze dried mixture (AK-BK) in molar ratios: 0:10; 1:9; 2:8; until to 10:0. The results showed that there were increasing antibiotic potency with extremity on (3:7); (5:5); (7:3) physical mixtures and on (3:7); (5:5); (6:4) freeze dried binary systems. The result is relevant with preliminary research that proved the extreme interactions on the molar ratios.
Identification of physical interaction between levodopa–benzerazide hydrochloride Nugrahani, Ilma; Asyarie, Sukmadjaja; Soewandhi, Sundani Noerono; Ibrahim, Slamet
INDONESIAN JOURNAL OF PHARMACY Vol 18 No 2, 2007
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1353.355 KB) | DOI: 10.14499/indonesianjpharm0iss0pp96-104

Abstract

The aim of this research was to investigate the possibility physical interaction occurred between of levodopa and benzerazide hydrochloride, which known as compounds used in combination for the the antiparkinson. The “cold contact methods” was used which was developed from Kofler contact method’s (hot stage methods). Thermograms Differential Scanning Calorimetry and difractograms X-Ray indicated that levodopa – benzerazide hydrochloride tent to form a peritecticum-molecular compound physical interaction with eutectics point at 4:6 and 9:1 molar ratios.Key words: benzerazide hydrochloride, cold contact method, levodopa, physical interaction.
Radioiodination of andrographolide and its biodistribution in mice for inflammatory tracer Levita, Jutti; A., Cahya Nova; Nawawi, As’ari; Ibrahim, Slamet
INDONESIAN JOURNAL OF PHARMACY Vol 21 No 4, 2010
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.965 KB) | DOI: 10.14499/indonesianjpharm0iss0pp258-265

Abstract

Andrographolide,  a  bioactive  component  of  Andrographis  paniculata (Burm.F)  Nees,  is  the  major  lactone  diterpenoidal  bicyclic  constituent  in  this plant  which  has  proven  to  exert  anti-inflammatory  activity  in  vitro  which  was occurred via  several mechanism, e.g inhibition of inducible nitric oxide synthase (iNOS),  inhibition  of  radical  oxygen  species,  and  inhibition  of  NF-kappaB activation. The labeling with radionuclide is often used for therapy, detection and quantification of metabolites in the body. Even though the metabolites are very low  in  concentration  they  can  be  detected  by  the  energy  they  emitted. Radionuclide  can  be  used as  radiotracer  to  detect  whether  drug  really interacts with  its  target.  The  objective  of  this  research  is  to  synthesize 131I-labelled andrographolide  and  to  study  its  biodistribution  in  mice  to  understand  the location  of  its  organ  target.  Indirect  radioiodination  of  andrographolide  wascarried  out  by  using  bromine  as  the  leaving  group  and  followed  by  fast iodination  at  40oC,  yielded  72.6  %  purity  of  the  labeled  compound.  Iodination was occurred through proton substitution at C-12. Then the andrographolide-131I was  injected  into  lateral  vein  of  mice’s  tail  to  study  its  biodistribution.  The compound  was  distributed  in  all organs  with the  highest  accumulation  occurred in  the  stomach  (16.87  %/gram  organ).  The  result  showed  that  inducing  the animals  with  LPS  caused  inflammation  in  the  stomach  and  increased  the production  of  prostaglandin  as  proven  by  the  distribution  of  the  radioligand  in that organ.Key words: andrographolide, radioiodination, anti-inflammatory, biodistribution
SENYAWA ASAM 2- METILESTER-1-H-PIROL-4-KARBOKSILAT DALAM EKSTRAK ETIL ASETAT BUAH SALAK VARIETAS BONGKOK SEBAGAI ANTIOKSIDAN DAN ANTIHYPERURICEMIA [Studies on 2-Methylester-1-H-Pyrolle-4-Carboxylic Acid Compound in Ethylacetate Extract of Snake Fruit Var Afrianti, Leni Herliani; Sukandar, Elin Yulinah; Ibrahim, Slamet; Adnyana, I Ketut
Jurnal Teknologi dan Industri Pangan Vol 21, No 1 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (371.817 KB)

Abstract

The aim of the study was to determine the antioxidant and antihyperuricemia activity of ethyl acetate extract of snake fruit (Salacca edulis Reinw.) var. Bongkok. The research methods used in this study comprised  of three stages. First stage, the isolation processes, consist ed of maceration, fractionation, and purification using several techniques of chromatography. The chemical structures of the isolated compounds were determined based on UV, IR, 1-D NMR, and 2-D NMR spectral data. The ethyl acetate extract of snake fruit var. Bongkok isolated was a new compound 2-methylester-1-H-pyrolle-4- carboxylic acid. In the second stage the antioxidant activity of the extract and the isolated compounds were measured by 1,1 diphenol  (DPPH) method. The antioxidant activity of the extracts and the isolated compounds were expressed as IC50, The ethyl acetate extracts at concentrations of 0.2, 2, 20, 200, 400, and 2000 µg/mL showed inhibition of 9.67, 4.47, 41.89, 96.06, 82.54, and 90.60 % respectively, with an IC50 of 1.6 µg/mL. Ascorbic acid standards at the same concentration range showed an  IC50 of 0.54 µg/mL. Meanwhile, at the same concentrations the 2-methylester-1-H-pyrolle-4-carboxylic acid showed free radical inhibition of 17.48, 21.48, 18.14, 31.87, and 62.34 % respectively, with an  IC50 of 3.27  µg/mL. During the third stage,  the antihyperuricemic properties of the extracts and the isolated compound were examinated in vitro using inhibition of xanthin oxidase method. The ethyl acetate extracts at concentrations of 0.01, 0.02, 0.2, 2, and 2000  µg/mL showed xanthin oxidase inhibition of 49.24, 49.58, 50.28 and 52.26 % respectively, with an  IC50 of 24.75 µg/mL. At the same concentrations, the 2-methylester-1-H-pyrolle-4- carboxylic acid, showed xanthin oxidase inhibition of 27.7, 30.5, 37.3, 50.27 and 50.55 % respectively, with an IC50 of 48.86 µg/mL.  Allopurinol as a standard drug showed an IC50 of 0.92 µg/mL.
IDENTIFIKASI INTERAKSI KIMIA FISIKA PADA KOMBINASI ANTIBIOTIKA AMOKSISILINA TRIHIDRAT - KALIUM KLAVULANAT DENGAN KALORIMETER LARUTAN DAN REKRISTALISASI Nugrahani, Ilma; Asyarie, Sukmadjaja; N.S., Sundani; Ibrahim, Slamet
Pharmaceutical Sciences and Research (PSR) Vol 4, No 3 (2007)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1320.559 KB) | DOI: 10.7454/psr.v4i3.3803

Abstract

Physico-chemical interaction of amoxicillin trihydrate - potassium clavulanate has been investigated. The method used in this experiment was solution calorimeter analysis that was collaborated by the re-crystallization observation. Amoxicillin-clavulanate were mixed on the variety ratios (0:10; 9:1; ...; 1:9; 0:10) and analyzed by solution calorimeter in the HCl 0,1 N and phosphate buffer pH 6,8 solution as the gastrointestinal liquids. After that, the re-crystallizations of amoxicillin-clavulanate on the variety ratios were analyzed by polarization microscope and digital camera. The results of observation showed that amoxicillin-clavulanate have the physical-chemical interactions in the ratios 3:7; 5:5; 7:3 respectively in both of pH models of gastrointestinal liquids.
Pengamatan Visual Tahap-Tahap Pembentukan Kristal Sistem Eutektikum Asetaminofen-Pseudoefedrin Hidroklorida Nugrahani, Ilma; Asyarie, Sukmadjaja; Soewandhi, Sundani Nurono; Ibrahim, Slamet
Pharmaceutical Sciences and Research (PSR) Vol 7, No 1 (2010)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (713.824 KB) | DOI: 10.7454/psr.v7i1.3447

Abstract

The aim of this research was to observe the recrystallization process of two component,acetaminophen-pseudoephedrine hydrochloride in ethanol and the thermal profiles.The visual data resulted from polarization microscope observation confirmed withthermograph DSC showed that the crystal habit was represented to the intrinsic characterrepresentative by thermodynamic properties of the binary system.The visualizationprocess proved that in different molar ratios, the binary system formed crystalshabit with different and step by step showed inhibit, to form polycrystalline until atmolar ratio 6:4 formed the fines single crystals mixture which was smaller than beforeco-recrystallization. Acetaminophen was observed as monoclinic hexagonal crystalswhile pseudoephedrine hydrochloride was observed monoclinic/orthorombic forms.Keywords: acetaminophen, crystals habit, ethanol, pseudoephedrine HCl.
Polimorfisasi dan Solvatomorfi Amoksisilina Trihidrat setelah Proses beku Kering Nugrahani, Ilma; Asyarie, Sukmadjaja; Soewandhi, Sundani Nurono; Ibrahim, Slamet
Pharmaceutical Sciences and Research (PSR) Vol 7, No 2 (2010)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (604.417 KB) | DOI: 10.7454/psr.v7i2.3452

Abstract

The polymorphismof amoxicillin has been identified after freeze drying process. Thefreeze dried amoxicillin have some specific physical properties different from their rawmaterial, that was proved by DSC, XRD, and polarize microscope. Exothermal curvefrom DSC thermogram changes to endothermal curve, diffractogram XRD changes todifferent profile, and the crystal shows different habit. All of data showed that freezedrying process improve amoxicillin to different crystal form which has higher meltingoxidation point and lower hydrate. The improvement of the crystal structure mightbe impact to change physical-pharmaceutical properties like dissolution, absorption,and change it antibiotic potency.Keywords: amoxicillin trihydrate, freeze drying, polymorphism.
Determination of Iodate and Iodide Content in Iodized Salt By Ion Pair High Performance Liquid Chromatography Method Cahyadi, Wisnu; Firman, Kurnia; Ibrahim, Slamet; Kartadarma, Embit
Jurnal Teknologi dan Industri Pangan Vol 15, No 1 (2004): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Two species of iodine, i.e. iodide and iodate in commercial iodized salt were determined using ion pair HPLC. From 15 samples analysed, the iodide and iodate content ranged from 24,05 ± 2,51 to 70,25 ± 3,78 ppm and from 31,43 ± 8,10 to 87,59 ± 0,44 ppm, respectively. The method used was found satisfactory in terms of precission, accuracy, sensitivity and selectivity, therefore the method seem acceptable for the determination of iodide and iodate content in iodized salt samples. Key words : Iodine species, determination of iodine species content, and ion-pair HPLC