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BIOLOGI REPRODUKSI INDUK IKAN KLON HITAM (Amphiprion percula) DI HATCHERY Setiawati, Ketut Maha; Gunawan, Gunawan; Hutapea, Jhon Harianto
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 4, No 2 (2012): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Jurnal Ilmu dan Teknologi Kelautan Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Ikan klon hitam (Amphiprion percula) sering juga disebut klon biak merupakan salah satu ikan hias laut yang memiliki nilai ekonomi tinggi yang dapat memijah dalam akuarium. Tujuan penelitian ini adalah untuk mengetahui beberapa aspek biologi reproduksi ikan klon hitam pada kondisi pemeliharaan akuarium. Sepasang induk dengan panjang total 4-9 cm diperlihara dalam akuarium ukuran 60x40x30 cm3. Jumlah induk yang digunakan sebanyak 18 pasang, setiap akuarium satu pasang induk. Pakan yang diberikan pertama-tama pakan buatan kemudian satu jam berikutnya pakan campuran jembret dan kopepoda sampai kenyang dengan frekuensi 2 kali sehari. Variabel yang diamati adalah ukuran induk, jumlah, dan derajat tetas telur. Hasil penelitian menunjukkan bahwa induk ikan betina memijah pada kisaran panjang 6,8-9,5 cm sedangkan jantan pada kisaran panjang 4,6-6,2 cm. Rata-rata jumlah telur per pemijahan sangat bervariasi yaitu 423±255 telur dengan frekuensi rata-rata 2,74 ± 0,38 kali/bulan. Pemijahan induk secara parsial dengan frekuensi maksimum 4 kali/bulan. Induk dengan ukuran 9,5 cm masih dapat aktif memijah dengan frekuensi 3 kali/bulan. Pasangan induk dapat menghasilkan jumlah telur di atas 700 telur/pemijahan. Penelitian ini juga menunjukkan bahwa induk ikan klon hitam dapat memijah sepanjang tahun di akuarium dengan jumlah telur dan frekuensi pemijahan yang bervariasi antar individu dan antar periode pemijahan. Derajat tetas rata-rata telur selama penelitian adalah 79,72±13,73 % dengan jarak waktu dari pemijahan pertama ke pemijahan berikutnya antara 7-14 hari.Kata kunci: Reproduksi, ikan klon hitam, Amphiprion percula, derajat tetas
REPRODUCTION BIOLOGY OF TRUE PERCULA CLOWN FISH Amphiprion percula IN HATCHERY Setiawati, Ketut Maha; Gunawan, Gunawan; Hutapea, Jhon Harianto
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 4, No 2 (2012): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Jurnal Ilmu dan Teknologi Kelautan Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

True percula clown fish (Amphiprion percula) is one of marine ornamental fish with high economic value and can spawn throughout the year in an aquarium. The aims of this research were to understand reproduction aspects of true percula clown fish broodstock in an aquarium. A pair of broodstock used in this experiment were 4-9 cm in total length and maintained in an aquarium of 60x40x30 cm3 dimension. Numbers of brrodstock were 18 pairs and each aquarium filled with one pair of broodstock. During the experiment, fish were fed with artificial feed first and one hour later with mysids shrimp and copepod until satiation twice a day. Parameters observed were broodstock size, numbers and hatching rate of eggs. The results showed, the size of spawned female were range from 6.6-9.5 cm and male from 4.6-6.2 cm. Average of eggs produced per spawning was varied 423±255 with the average of spawning frequency was 2.78±0.38 times/month. Broodstock was spawn partially with the maximum spawning frequency of single broodstock was 4 times/month. The biggest female with size of 9.5 cm was still productive and spawn 3 times/month. There was a pair of broodstock which can produce more than 700 eggs/spawning. This results indicated that true percula clown fish can spawn throughout the year in the aquarium with varied of eggs number and spawning frequency for each individual and spawning period. The average of hatching rate during the experiment was 79.72±13.73 % with range between first to the next spawning 0f 7-14 day.Keywords: reproduction, true percula clown fish, Amphiprion percula, hatching rate
REPRODUCTION AND LARVAL REARING OF SANDFISH (Holothuria scabra) Sembiring, Sari Budi Moria; Wibawa, Gigih Setia; Giri, I Nyoman Adiasmara; Hutapea, Jhon Harianto; Haryanti, -
Marine Research in Indonesia Vol 43, No 1 (2018)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.996 KB) | DOI: 10.14203/mri.v43i1.267

Abstract

The natural stock of sea cucumber Holothuria scabra (known as sandfish) has been declining in both population and size, making it difficult to collect broodstock and juvenile sandfishes for aquaculture. This research aims to evaluate the reproduction of domesticated broodstock (F-1) and performance of the juveniles (F-2). Broodstock were reared in two rectangular concrete tanks with a dimension of 190 x 290 x 70 cm3. There were 12 individuals (7 males and 5 females) of broodstock (F-1) with total length and mean body weight of 12.0 ± 1.21 cm and 122.6 ± 32.37 g, respectively. Six broodstock were put in each tank and fed with compressed benthos at 4% biomass daily in the afternoon. During our experiment, domesticated sandfish broodstock successfully spawned twice. The first spawning (occurred in January 2017) had one female spawned that released 1,350,000 eggs with a hatching rate of 57.4%. Another broodstock spawned in April 2017 and produced 3,280,000 eggs with a hatching rate of 78.66%. The growth performance of 170 days-old juveniles (F-2) shows a total length of 5.66 ± 0.90 cm and a mean body weight of 10.08 ± 2.07 g. The survival rates are 5.19% and 8.68% for juveniles spawned in January and April, respectively. We conclude by showing that sandfish could be domesticated to produce seeds for further aquaculture development.
EMBRYO DEVELOPMENT OF YELLOWFIN TUNA (Thunnus albacares) AT DIFFERENT INCUBATION TEMPERATURE Hutapea, Jhon Harianto
Indonesian Aquaculture Journal Vol 2, No 2 (2007): (December 2007)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.839 KB) | DOI: 10.15578/iaj.2.2.2007.99-105

Abstract

The experiment was conducted in order to figure out the effect of incubation temperature on embryonic development of yellowfin tuna, Thunnus albacares eggs. Five different incubation temperatures were applied as treatments, i.e.: 24°C, 26°C, 28°C, 30°C, and 32°C with 3 replicate each. Ten micro plates with lid (IWAKI, Japan) were used; each has 6 well and 10 mL volumes. Five micro plates were used for experiment and five for balance on shaker. Three well of each micro plate were filled with 8 mL ultra violet sterilized sea water and 50 fertilized eggs. Temperature was set using Multi Thermo Incubator which has 5 level racks. Temperatures were set from the lowest to the highest on bottom to upper rack order. To maintain eggs dispersed in the medium, shaker on each rack was operated at 150 RPM. The embryo was monitored every 30-60 minutes depends on embryonic stage development using Microscope which was connected to Digital Camera DXM 1200F. Image analyses by Image Analyzer Program. The results showed, incubation temperature was significantly affect (P<0.05) embryonic development and hatching time of yellowfin tuna (Thunnus albacares) eggs. Optimum incubation temperature for embryo development and hatching was 28°C. Decreased on incubation temperature slows down embryo development at all stages, and vice versa, increased on incubation temperature accelerates embryo development.
GENETIC IDENTIFICATION FOR TUNA AND RAINBOW RUNNER CAPTURE IN NORTH BALI WATERS Permana, Gusti Ngurah; Hutapea, Jhon Harianto; Haryanti, Haryanti; Sembiring, Sari Budi Moria; Nakazawa, Akio
Indonesian Aquaculture Journal Vol 1, No 1 (2006): (June 2006)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (924.62 KB) | DOI: 10.15578/iaj.1.1.2006.29-34

Abstract

Gondol Research Institute for Mariculture identification of tuna and rainbow runner was an objective in this current study. Samples of five species were collected from territorial water of North Bali. The method used in this study was allozyme electrophoresis. The results showed that buffer of CAPM-6 (citric acid aminoprophylmorpholine) resulted in a sharp and clear banding pattern. The species could be differentiated in six diagnostic isozyme patterns Idh* (isocitrate dehydrogenase), 6Pgd* (6 phosphogluconate dehydrogenase), Gpi* (glucose phosphate isomerase), Mdh* (malate dehydrogenase), Est* (esterase), and Sp* (sarcoplasmic protein). All species were in Hardy-weinberg equilibrium. Heterozygosities of species were ranged from 0.00 to 0.099. Yellowfin tuna has the highest heterozigosity compared with the other species. Clustering samples according to pairs revealed that genetic distance of Bullet tuna (A. rochei) and Eastern little tuna (E. affinis) had small value (0.001). By contrast, the largest value was observed between yellowfin tuna, T. albacares and rainbow runner, E. bipunnulata (0.007). This value indicated that Bullet tuna (A. rochei) and Eastern little tuna (E. affinis) closed relation, while among yellowfin tuna, skipjack tuna, and rainbow runner, were separated phylogenically.
MORPHOLOGICAL DEVELOPMENT OF Napoleon WRASSE, Cheilinus undulatus LARVAE Hutapea, Jhon Harianto; Slamet, Bejo
Indonesian Aquaculture Journal Vol 1, No 2 (2006): (December 2006)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1048.736 KB) | DOI: 10.15578/iaj.1.2.2006.145-151

Abstract

To improve the survival rate, napoleon wrasse larval rearing trial was conducted at Research Institute for Mariculture, Gondol-Bali in 2003. The trial aims at assessing initial feed for larvae, food habit, and morphological development from early larval stage to juvenile. The results showed that chicken egg yolk could be applied as initial feed and followed by rotifer, Artemia and mysid (Mesophodopsis sp.). Three swimming behavior of larvae were observed, drifting, free swimming and hiding on the substrate as larvae develop. Digestive system development, simple tube like, transition stage and coiled where digestive system could be distinguished between stomach, intestine and rectum.
KAJIAN BIOREPRODUKSI DAN KOMPOSISI PROKSIMAT DAGING IKAN TUNA SIRIP KUNING (Thunnus albacares) DARI BEBERAPA PERAIRAN DI INDONESIA Hutapea, Jhon Harianto; Andamari, Retno; Giri, I Nyoman Adiasmara; Permana, Gusti Ngurah
Jurnal Riset Akuakultur Vol 1, No 3 (2006): (Desember 2006)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1562.994 KB) | DOI: 10.15578/jra.1.3.2006.325-336

Abstract

KAJIAN BIOREPRODUKSI DAN KOMPOSISI PROKSIMAT DAGING IKAN TUNA SIRIP KUNING (Thunnus albacares) DARI BEBERAPA PERAIRAN DI INDONESIA
PEMELIHARAAN INDUK IKAN TUNA SIRIP KUNING, Thunnus albacares DALAM BAK TERKONTROL Hutapea, Jhon Harianto; Permana, Gusti Ngurah; Setiadi, Ananto
Jurnal Riset Akuakultur Vol 5, No 1 (2010): (April 2010)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1430.663 KB) | DOI: 10.15578/jra.5.1.2010.79-90

Abstract

Intensifikasi penangkapan ikan tuna baik yang langsung dipasarkan maupun dibesarkan dalam usaha budidaya, berpengaruh negatif terhadap kelestarian populasi ikan ini di alam. Dengan demikian upaya perbenihan secara buatan perlu dilakukan untuk mengurangi tekanan terhadap populasi alam. Balai Besar Riset Perikanan Budidaya Laut, Gondol telah merintis perbenihan ikan tuna sirip kuning (Thunnus albacares) sejak tahun 2003 bekerjasama dengan Overseas Fishery Cooperation Foundation (OFCF) Jepang. Induk ikan tuna telah berhasil dibesarkan dan dipijahkan dalam bak beton bervolume 1.500 m3 secara terkontrol, dengan sistem pergantian air semi tertutup. Pakan yang diberikan berupa ikan layang dan cumi-cumi sekitar 2,5% biomassa per hari. Pemijahan pertama terjadi pada bulan Oktober tahun 2004, ukuran induk diperkirakan lebih dari 9,138 kg atau panjang cagak lebih dari 82 cm dengan perkiraan umur sekitar 2 tahun. Puncak pemijahan terjadi pada tahun 2005 dan 2006 dengan frekuensi pemijahan masing-masing lebih dari 100 kali. Pemeliharaan induk ikan tuna dengan kepadatan 0,66 kg/m3 belum dapat dikatakan sebagai kepadatan maksimum dan peningkatan kematian cenderung akibat ruang gerak yang semakin sempit seiring dengan pertumbuhan induk. Namun demikian kendala yang ditemukan dalam pemeliharaan induk adalah kematian akibat menabrak dinding bak sedangkan kendala dalam pemeliharaan larva adalah serangan endoparasit pada telur
DETERMINASI JENIS KELAMIN PADA IKAN KERAPU SUNU (Plectropomus leopardus) DENGAN UJI SEROLOGI Sembiring, Sari Budi Moria; Priyono, Agus; Hutapea, Jhon Harianto; Setiadharma, Tony
Jurnal Riset Akuakultur Vol 8, No 2 (2013): (Agustus 2013)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.095 KB) | DOI: 10.15578/jra.8.2.2013.181-189

Abstract

Dalam rangka mendukung kegiatan budidaya, maka penentuan jenis kelamin ikan menjadi sangat penting dalam program pemijahan khususnya pada jenis ikan yang hermafrodit. Penelitian ini bertujuan untuk menentukan jenis kelamin ikan kerapu sunu menggunakan uji serologi dalam mendukung manajemen pemijahan dan pengembangan perbenihan ikan kerapu sunu. Penelitian ini dilakukan dengan metode ELISA dan western blot. Kit estradiol-17β dan 11-KT testosterone digunakan dalam metode ELISA, sedangkan antibodi Cyp19a1a (CT), Z-fishTM digunakan dalam metode western blot. Gonad dari induk kerapu sunu yang mati juga dianalisis secara histologis. Sampel darah diambil dari semua ikan (47 ekor) dengan kisaran bobot ikan uji 1,2-3,0 kg. Analisis kandungan testosteron dilakukan untuk semua sampel dan hanya 24 sampel dianalisis estradiol, keduanya dengan metode ELISA. Delapan sampel dianalisis estradiolnya dengan metode western blot. Berdasarkan kadar testosteron dan estradiol-17β dalam darah, menunjukkan sebanyak 12 ekor (37,5%) positif berkelamin jantan dari 32 ekor yang dianalisis, sedangkan berdasarkan kadar estradiol sebanyak tujuh ekor (29,16%) dari 24 ekor yang dianalisis merupakan ikan yang berjenis kelamin betina. Dengan metode western blot, dari delapan sampel yang dianalisis hanya tiga sampel (No Tagging 421048486E; 42135F1A5D; 42102G7A22) yang positif berjenis kelamin betina. Berdasarkan data histologis menunjukkan bahwa ukuran (panjang dan bobot) belum dapat menentukan jenis kelamin dari ikan kerapu sunu secara morfologi. Dari kedua metode yang digunakan untuk determinasi jenis kelamin induk ikan kerapu sunu, metode western blot memberikan hasil yang lebih sensitif dan spesifik daripada metode ELISA.
INDUCED FUCTIONAL MALE OF CORAL TROUT GROUPER (Plectropomus leopardus) USING 17α-METHYLTESTOSTERONE HORMONE Sembiring, Sar Budi Moria; Hutapea, Jhon Harianto; Wardana, Ida Komang; Muzaki, Ahmad; Mastuti, Indah
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (659.271 KB) | DOI: 10.15578/iaj.8.2.2013.101-106

Abstract

The success of grouper seeds production is depends on the availability of qualified broodstock. The nature of grouper is protogynous hermaphrodite, causing difficulties to mantain female and a bit difficult to get male broodstock, one possibility to accelerate sex revers is by hormone manipulation. The aim of this experiment was to find effectiveness of 17α-methyl testosterone hormone to produce coral trout grouper (Plectropomus leopardus) functional male. The experiment was conducted in floating net cage by using 6 net cages with size of 2 m x 2 m x 2 m at density of 25 fish/cage, size of fish were 377.27±21.49 g. The fishes were treated by hormone implantation at concentration of 50 μg/kg body weight and without hormone implantation as a control with 3 replicates. The results showed that the highest concentration of testosteron in fish blood (1.144±0.135 pg/mL) was detected after four months of hormone treatment, but the concentration of testosteron in fish blood declined after 8th months of treatment. The treated fish with hormone grew faster than control. Based on histological analysis of gonad, female gonado somatic index was higher for treated fish compare to control. Its seems that hormone tratment lead to promote development of female maturity and than sex reverse into male become faster.