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PENGARUH SUPLEMENTASI HIDROLISAT BULU AYAM DAN MINERAL ORGANIK TERHADAP KECERNAAN ZAT MAKANAN, PERTAMBAHAN BOBOT BADAN, DAN EFISIENSI RANSUM KAMBING PERANAKAN ETAWAH JANTAN Muhtarudin, Muhtarudin; Husni, Ali
Majalah Ilmiah Peternakan Vol 10, No 3 (2007)
Publisher : Majalah Ilmiah Peternakan

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ABSTRAK Tujuan penelitian ini adalah untuk mengevaluasi penggunaan suplementasi hidrolisat bulu ayam, mineral, makro organik (Ca, Mg organik) serta mikro organik (Zn, Cu, Cr, Se organik) guna meningkatkan kecernaan zat-zat makanan, pertambahan bobot badan, dan efisiensi ransum kambing peranakan Etawah jantan. Percobaan menggunakan kambing peranakan Etawah jantan sebanyak 20 ekor. Rancangan yang digunakan adalah rancangan acak kelompok dengan 4 perlakuan dan 5 ulangan. Perlakuan yang dicobakan adalah: R0 = 40% rumput gajah + 60% konsentrat, R1 = R0 + 3 % tepung bulu ayam, R2 = R1 + Mineral Makro-organik (Ca, Mg organik), R3 = R2 + Mineral Mikro-organik (Zn,Cu, Cr, Se organik). Hasil penelitian menyimpulkan bahwa perlakuan campuran bulu ayam, Ca-PUFA, Mg-PUFA (mineral makro organik) dan Zn, Cu, Se, dan Cr, lisinat (mineral mikro organik) atau R3 dapat meningkatkan kecernaan bahan organik, energi, pertambahan bobot badan, dan efisiensi ransum dari kambing peranakan Etawah jantan. THE EFFECT SUPLEMENTATION OF HYDROLYZED OF FEATHER MEAL AND ORGANIC MINERAL ON NUTRIENTS DIGESTIBILITY, DAILY GAIN, AND RATIONS EFFICIENCY OF MALE ETAWAH CROSS GOATS ABSTRACT The objective of research were to evaluate the effect of supplementation of hydrolyzed of feather meal, organic macro mineral (Ca and Mg-Polyunsaturated Fatty Acid /PUFA) and organic micro mineral (Zn, Cu, Cr, and Se-lysinat) to improve nutrient digestibility, daily gain and rations efficiency of male Etawah cross goats. The research were used 20 male Etawah cross goats. Random block design were used this research was used, with 4 treatments and 5 replications. The treatments were arranged : R0= 40 % of forage + 60% concentrates; R1= R0 + 3% of hydrolyzed of feather meal ; R2= R1 + organic macro mineral (Ca and Mg -PUFA); R3 = R2+ organic micro mineral (Zn,Cu, Cr, and Se-lysinat). The results showed that mixing treatment of hydrolyzed feathers meal, organic macro and micro mineral (Ca and Mg-Polyunsaturated Fatty Acid /PUFA and Zn, Cu, Cr, and Se-lysinat)/R3 had significantly effect of increasing organic digestibility, digestible energy, daily gain and rations efficiency of male Etawah cross goats.
PROPAGASI TANAMAN ADAS SECARA IN VITRO Kristina, Natalini Nova; Husni, Ali; Gati, Endang
Warta Tumbuhan Obat Indonesia Vol 4, No 1 (1998)
Publisher : Warta Tumbuhan Obat Indonesia

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Telah dilakukan penelitian perbanyakan vegetatif melalui kultur in vitro tanaman adas (Foeniculum vulgare Mill.). Penelitian mencakup tahap pertukaran, perakaran dan aklimatisasi.
Peningkatan Toleransi Kedelai Sindoro terhadap Kekeringan Melalui Seleksi In Vitro Husni, Ali; Kosmiatin, M.; Mariska, Ika
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 34, No 1 (2006): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

In vitro selection of embryogenic cell mass is one alternative to improve drought tolerance in plants. Embryogenic cell callus of soybean were radiated by Gamma ray (400 rad) to produce mutation. The radiated cell  were tested with PEG (0, 10, 20 and 30 %) for drought stress tolerance. After selection, cells which tolerant to PEG were regenerated to produce somatic embryo structure, somatic seed and plantlet. Acclimatization was done in a greenhouse and analysis of proline was done at generation 1 (G1). The purpose of the experiment was to get soybean somatic seed which tolerant to drought stress. Results of experiment showed that 39.7 % embriogenic callus were produced. The higher the concentration of PEG, the higher the death of cell/callus. The rate of producing somatic embryo structure was 4.9 at 0 % PEG; 2.85 at 10 % PEG; 1.6 at 20% PEG and 0.6 at 30% PEG. Number of somatic seed which developed in regeneration medium (S11) were 79 from 0% PEG;  35 from 10% PEG; 29 from 20% PEG, and 15 from 30% PEG. Somatic seed produced 15 planlets from PEG 0%; 6 planlets from PEG10%; 4 planlets from PEG 20%. Based of proline content, all of G1 somaclones were more tolerant  than the mother plant.     Key words : Soybean, in vitro selection, PEG, regeneration, acclimatization and dry land.                     
Induksi Tetraploid Tunas Pucuk Jeruk Siam Simadu (Citrus nobilis Lour) Menggunakan Kolkisin secara In Vitro Yulianti, Fitri; Purwito, Agus; Husni, Ali; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 42, No 2 (2014): Jurnal agronomi indonesia
Publisher : Bogor Agricultural University

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ABSTRACT Seedless fruit is one of the criteria necessary to improve the quality of Simadu tangerine. The most effective method to obtain seedless triploid cultivars is hybridisation between tetraploid and diploid parents. Simadu tangerine is diploid plant. Tetraploid Simadu tangerine can be obtained with doubling chromosome using colchicines. The aim of this research was to obtain tetraploid Simadu tangerine shoot which would serve as parent to produced seedless Simadu tangerine. Shoot-tips of Simadu tangerine without leaves were treated with colchicines at four different concentrations (0%, 0.1%, 0.2%, and 0.3%) for 3 hours.  The results showed that the high concentration reduced survival rate. The colchicine treatments reduced growth of shoot-tip of Simadu Tangerine. The leaves of colchicines treated shoots were thicker than control. Leaves from control (0% colchicine) and 0.1% colchicine treated shoots had 8.67 and 18.25 chloroplasts per pair of guard cells. Leaves from 0.1% colchicine treated shoots had lower stomatal density than those of the control (0% colchicine) shoots. Leaves from 0.1% colchicine treated shoots had larger stomatal sizes than those of the control (0% colchicine) shoots. Therefore, it appeared that 0.1% colchicine treatment resulted in tetraploid Simadu Tangerine Shoot.  Keywords: chloroplasts, doubling chromosomes, and stomatal size
PENERBITAN BANK GARANSI PADA LEMBAGA PERBANKAN MENURUT UNDANG – UNDANG NOMOR 10 TAHUN 1998 Husni, Ali
DEDIKASI JURNAL MAHASISWA Vol 2, No 1 (2014)
Publisher : DEDIKASI JURNAL MAHASISWA

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AbstractProvision of Bank Guarantee is one of the functions of the Bank, in addition to the function of providing a wide range of banking services. As a policy, the Bank Guarantee given to customers of the Bank with the aim of providing assistance that are pertinent customer support who will perform work tasks that are very limited and selected. Bank to Bank Guarantee issuing a written acknowledgment that it agreed to adhere to the insured in order to fulfill the obligation secured within a certain period of time and with certain requirements in the form of payment of a certain sum of money in the future is assured if it did not fulfill its obligations to the recipient warranty or guarantee in the future if it turns default (default). Provision of Bank Guarantee is one of the functions of the Bank, in addition to the function of providing a wide range of banking services. As a policy, the Bank Guarantee given to customers of the Bank with the aim of providing assistance that are pertinent customer support who will perform work tasks that are very limited and selected.
UKURAN-UKURAN TUBUH SAPI KRUI JANTAN DAN BETINA DI KABUPATEN PESISIR BARAT LAMPUNG Hamdani, Muhammad Dima Iqbal; Adhianto, Kusuma; -, Sulastri; Husni, Ali; -, Renitasari
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 17, No 2 (2017)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.24198/jit.v17i2.16068

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AbstrakPenelitian ini bertujuan untuk mengetahui ukuran-ukuran tubuh sapi Krui jantan dan betina di Kabupaten Pesisir Barat provinsi Lampung.  Penelitian ini dilakukan pada November-Desember 2016.  Sampel yang diamati sebanyak 55 ekor sapi jantan dan 71 ekor sapi betina.  Metode survey digunakan dalam penelitian ini, dan sampel tersebut ditentukan dengan menggunakan purposive sampling.  Data dianalisis dengan uji t.  Peubah yang diamati meliputi bobot badan, lingkar dada, panjang badan, dan tinggi pundak.  Hasil penelitian menunjukan bahwa rata-rata bobot badan sapi Krui jantan dan betina poel 0 (125,4±117 kg), poel 1 (148,6±136,2 kg), poel 2 (189,6±176,1 kg), poel 3 (234,5±208,4 kg), lingkar dada poel 0 (118±110 cm), poel 1 (132,9±128,3 cm), poel 2 (134,5±129,5 cm), poel 3 (137±130,3 cm), panjang badan poel 0 (103±97,2 cm), poel 1 (110,3±104,1 cm), poel 2 (124,6±109,8 cm) poel 3 (133,6±127,4 cm), sedangkan rata-rata tinggi pundak poel 0 (91,3±86,7 cm), poel 1 (102,5±97,8 cm), poel 2 (105,1±101,1 cm), poel 3 (113±104,7 cm).  Hasil penelitian ini menunjukkan bahwa bobot badan, lingkar dada, panjang badan, dan tinggi pundak sapi Krui jantan dan betina pada kelompok umur yang sama menunjukkan perbedaan yang nyata (P<0,05).  Kata Kunci : Sapi Krui, Sifat kuantitatif, Bobot badan, Lingkar dada, Panjang badan, Tinggi pundak.
Induksi Mutasi Pada Populasi Kalus Embriogenik Jeruk Siam Pontianak Dengan Sinar Gamma Husni, Ali; Kosmiatin, Mia
Zuriat Vol 23, No 2 (2012)
Publisher : Zuriat

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Jeruk siam Pontianak (Citrus nobilis var. Microcarpa) merupakan salah satu jeruk unggulan di Indonesia. Rasa jeruk Pontianak ini cukup manis tetapi belum sesuai dengan kategori yang diinginkan pasar dunia untuk dikonsumsi dalam keadaan segar karena mempunyai biji yang relatif banyak (15-20 biji per buah) dan mempunyai warna kulit yang belum begitu menarik sehingga kalah bersaing dengan jeruk produk impor. Trend kebutuhan pasar dunia akan buah jeruk keprok/siam segar saat ini perlu memenuhi kategori buah yang tidak berbiji (seedless), mudah dikupas (easy peeling), dan mempunyai tipe mandarin dengan warna yang menarik (pigmented). Jeruk mempunyai sifat heterozigositas yang tinggi, poligenik dengan masa juvenile yang panjang. Untuk melakukan perbaikan mutu buah melalui pemuliaan konvensional membutuhkan waktu yang lama dan memerlukan biaya yang besar. Pemuliaan mutasi merupakan salah satu alternatif penyelesaian masalah. Induksi mutasi pada populasi sel embriogenik jeruk siam Pontianak dilakukan dengan sinar Gamma pada dosis 0, 10, 20, 30, dan 40 Gy. Regenerasi populasi kalus embriogenik yang telah diradiasi dilakukan melalui embriogenesis somatik. Untuk mendewasakan embrio somatik dilakukan pada media MW dengan penambahan ABA (1,0; 1,5; 2,0; dan 2,5 mg/l) dan MW medium + EM 500 mg/l + ABA (0,0; 1,0; 1,5; 2,0; dan 2.5 mg/l) untuk perkecambahan samapai terbentuk plantlet dengan menambahkan GA3 0,5 mg/l. Hasil penelitian ini menunjukkan bahwa iradiasi sinar gamma dapat mempengaruhi  pertumbuhan, kecepatan regenerasi kalus menjadi plantlet, dan morfologi. Dari hasil  penelitian dapat disimpulkan bahwa iradiasi sinar gamma dapat memperlihatkan keragaman berdasarkan pertumbuhan, morfologi, dan kecepatan regenerasi kalus menjadi plantlet.
Embriogenesis dan Desikasi Embrio Somatik Jeruk Keprok Batu 55 (Citrus reticulata Blanco.) untuk Meningkatkan Frekuensi Perkecambahan Fathur Rahmi, Atika; Purwito, Agus; Husni, Ali; Dinarti, Diny
Jurnal Hortikultura Indonesia Vol 8, No 2 (2017): Jurnal Hortikultura Indonesia
Publisher : Perhimpunan Hortikultura Indonesia dan Departemen Agronomi dan Hortiluktura

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Abstract

ABSTRACTIn vitro breeding technique of citrus is effective when optimum explant regeneration method is obtained. Low germination frequency and high abnormality were barrier in citrus somatic embryogenesis. This research aimed at optimizing somatic embryogenesis in Tangerine var. Batu 55. This research consisted of 3 experiments. First experiment was maturation of embryogenesis, using Completely Randomized Design (CRD) method. Modified MS+MW was used as basic media added with 500 mg L-1 malt extract (control) and addition of 3 mg L-1 BAP, and 2.5 mg L-1 ABA as treatments. Second experiment was SE (cotyledonary phage) desiccation. Factorial CRD used in two factors. First factor was poly-ethylene-glicol/PEG 8000 (0, 2.5, 5, 7.5 and 10%), while second factor was immersed periods (control, 3, 6, and 9 hours), in desiccant solution (base medium + PEG). Third experiment was studied of plantlet growth and development planlets. Based on CRD 2 factor method, the first factor was PEG concentrations from the second experiment. Second factor were active charcoal treatments (with or without), in basic media. The result showed that 2.5 mg L-1 ABA produced has highest mature somatic embryo (SE). Desiccation for 9 hours, induced the highestt germination frequencies (90.29%). The best growth of plantlets shown in previous experiments immersed desiccant PEG 2.5% for 9 hours, and cultured in basic media with 2 g L-1 of activated charcoal.Keywords: desiccant, embryogenic callus, maturation, PEG 8000, somatic embryo ABSTRAK Pemuliaan tanaman melalui teknik in vitro efektif bila metode regenerasi eksplan optimum telah diperoleh. Rendahnya frekuensi perkecambahan dan tingginya abnormalitas, menjadi kendala pada embriogenesis somatik jeruk. Penelitian terdiri atas 3 percobaan paralel, bertujuan mengoptimalkan metode embriogenesis somatik jeruk, khususnya Keprok Batu 55. Percobaan pertama pematangan kalus embriogenik menggunakan Rancangan Acak Lengkap (RAL) satu faktor, dengan perlakuan penambahan ZPT (kontrol, 3 mg L-1 BAP, dan 2.5 mg L-1 ABA) pada media dasar (MS modifikasi vitamin MW) diperkaya 500 mg L-1 ekstrak malt. Percobaan kedua desikasi embrio somatik (fase kotiledon) menggunakan RAL dua faktor. Faktor pertama konsentrasi poly-ethylene-glicol/PEG 8000 (0, 2.5, 5, 7.5 dan 10%), dan faktor kedua waktu perendaman (kontrol, 3, 6, dan 9 jam) pada larutan desikan (media dasar + PEG). Percobaan ketiga mempelajari pertumbuhan dan perkembangan planlet, menggunakan RAL dua faktor. Faktor pertama konsentrasi PEG planlet pada percobaan kedua, dan faktor kedua perbedaan media dasar (tanpa dan dengan arang aktif). Hasil percobaan menunjukkan penambahan 2.5 mg L-1 ABA menghasilkan maturasi embrio somatik terbaik. Desikasi 9 jam menghasilkan frekuensi perkecambahan 90.29%. Pertumbuhan terbaik ditunjukkan planlet yang pada percobaan sebelumnya direndam 9 jam desikan PEG 2.5%, dan dibesarkan pada media dasar dengan 2 g L-1 arang aktif.Kata kunci : desikan, embrio somatik, kalus embriogenik, PEG 8000, pematangan
Perkecambahan dan Perbanyakan Gaharu secara In Vitro Kosmiatin, Mia; Husni, Ali; Mariska, Ika
Jurnal AgroBiogen Vol 1, No 2 (2005): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

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Agarwood (Aquilaria malaccensis Lank) is one of the forest wood that are continously exploited. Currently, the Indonesian export of agarwood is decreasing because its population is endangered by excessive logging. Agarwood propagations need technology for reproduction of agarwood seedlings and their fungal inoculum. In vitro technique for germination of recalsitrant seeds and micropropagation are technologies that can be used for propagation of agarwood seedlings. An experiment was done to develop techniques for in vitro germination and micropropagation of agarwood. The in vitro germination was done using two different techniques. Firstly, sterile seeds were germinated on an MS medium + 50 mg/l PVP, 50 mg/l GA, and 1 mg/l BA or kinetin. Secondly, sterile seeds were germinated on basal medium of MS, 1/2 MS medium, MS medium without vitamins, as well as on MS medium without pyridoxine, nicotinic acid and WPM. Shoot initiations and multiplications were done on MS and 1/2 MS media containing 1, 3, or 5 mg/l BA. The explants used were cotyledone nodes, terminal shoots, single node with leaf, and sinle node without leaf. The results showed that the seed germination rate on the different media ranged from 7,14 to 50%. The seed germination rate on the MS medium without vitamis was the highest. The best explants for shoot induction and multiplication was single node with leaf which was cultured on MS + 1 mg/l BA.
Peningkatan Toleransi Alumunium pada Jeruk Batang Bawah dengan Teknik Seleksi In Vitro Berulang Kosmiatin, Mia Toruan; Yunita, Rosa; Husni, Ali
Jurnal AgroBiogen Vol 6, No 1 (2010): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

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Aluminum Tolerance Improvement of Rootstock Citrusthrough Repeated In Vitro Selection. Mia Kosmiatin,Rosa Yunita, and Ali Husni. National orange productivitywas trend to decrease because of pathogen attack andreducing of planting area. One of alternative ways topreserve and increase orange productivity was usingmarginal soil mainly acid soil. This matter pushed thebreeder to prepare tolerant rootstock and stable in the acidsoil. In vitro culture technique was effective and efficientmethods to produce tolerant and stable rootstock in acid soilthrough simulation of acid soil with addition of highaluminum and low pH in the medium. By the simulation theselection could be done in cell level, so cell was selectedafter induction of variation. A rootstock which highcompatibility with scion, useful rooting, and aluminumtolerance could be increased orange productivity throughacid soil development. The research was conducted in 3phase: (1) induction of embryogenic calli, (2) improvementof genetic variation through mutation, and (3) In vitroselection with AlCl3.6H2O for aluminum and low pH tolerant.Immature embryos of rootstock were use as explant. Theresult showed that the best embryogenic calli were inducedon MS basal medium with MW vitamin + NAA 7,5 mg/l +kinetin 0,5 mg/l. Before selection, 1.000 rad dosage was themost tolerant dosage to growth embryogenic calli. Afterselection, 2.000 rad dosage was the best dosage to produceshoots which stable tolerant to aluminum. Selected 88mutant shoots were produced after three times selection onthe same medium which AlCl3.6H2O added at low pH.