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Deteksi Dini Penyakit Tumor Sel Darah Myelosit Leukosis melalui Pemeriksaan Ulas Darah Agungpriyono, Dewi Ratih; Huminto, Hernomoadi; Estuningsih, Sri; Satyaningtijas, Aryani Sismin
Jurnal Ilmu Pertanian Indonesia Vol 12, No 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Myelocytes blood cell tumor in chicken is a disease caused by retrovirus, avian leukosis virus subgroup J (ALV-J). The virus has the same group as human retrovirus (HIV) which caused AIDS, but the avian type possesses oncogenic properties, that could induce cell transformation and tumor formation. ALV-J stimulates the bone marrows myelocytes and transforms them into tumor cell myelocytoma. The tumor cells then metastasis through the circulatory system causing myeloid leukosis and tumor cells accumulation in various internal organs or myelocytomatosis. This study was done on the base of the leukosis behavior of the tumor. The finding of the metastasis tumor cell in the blood smear examination is thought could be use as the diagnostic clue of the disease. Blood smear from sick chickens are collected and stained with some chemical staining substance such as may grunwald-giemsa, hematoxyllin eosin, periodic acid Schiff, congo red, toluidine blue, and sudan black B. Cytochemistry character of the blood cells was observed using light microscope. The result showed that myelocytes granules were best observed using hematoxyllin eosin, periodic acid Schiff, congo red and toluidine blue while may grunwald-giemsa, and sudan black B could not differentiate the granules. By this method, the field veterinarian will able to screen the suspected chicken flock for myeloid leukosis earlier than the occurrence of tumor formation.
Gongylonema ingluvicola ransum, 1904 pada ayam buras di Medan Sumatera Utara Siahaan, Panal M.; He, Simon; Huminto, Hernomoadi; Sugiri, Nawangsari
Hemera Zoa Vol 77, No 1 (1995): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Dari 96 ekor sampel ayam buras yang diperoleh dari Kotamadya Medan dan sekitarnya untuk keperluan survai cacing parasitik ditemukan 42 ekor (43,75%) diantaranya mengandung cacing Gongylonema ingluvicola di dalam temboloknya. Cacing ini ditemukan pada sampel ayam buras dari semua lokasi yang disurvai dengan rataan derajat infeksi 14 ekor cacing per ayam. Cacing ini membentuk rangkaian lipatan-lipatan pada mukosa yang agak teratur dan seragam, berupa terowongan yang melingkar-lingkar dan mengakibatkan penebalan mukosa tembolok ke arah lumen. Panjang cacing jantan antara 16 - 21 mm dengan rataan 19 mm dan diameter 225 - 255 µm; panjang cacing betina antara 31 - 54 mm dengan rataan 4 1,6 mm dan diameter 3 15 - 345 µm. Cacing jantan mempmyai ale (pelebaran kutikula ke arah lateral serupa sayap) pada sisi kiri dan kanan yang tidak simetris. Ale kiri disokong oleh 7 buah papila (tonjolan kutikula berbentuk duri) sedangkan ale kanan disokong oleh buah 5 papila. Pada cacing betina, vulvanya terletak di bagian posterior tubuh.   
The macroscopic and microscopic patology changes on broiler infected with local reovirus isolate Wahyuwardani, Sutiastuti; Huminto, Hernomoadi; Parede, Lies
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 1 (2005): MARCH 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

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Abstract

Local reovirus isolate is the virus which could be isolated from runting and stunting syndrome. The ability of local reovirus isolate to induce runting and stunting syndrome in broilers chicken was investigated. The day old chicks (doc) were infected with local reovirus isolate assessed clinically and pathologically at 1, 2 and 3 weeks post inoculation. A total of 40 DOC were divided into two groups. The first group (20 doc) was orally infected with ± 2 x 103 local reovirus isolate particle and the other used as negative control group. The results showed that the isolate caused wet droppings, stunting, enteritis, pancreatitis, malabsorbtion, bursal atrophy and spleenic hypertrophy, which similar to runting and stunting syndrome (RSS) disease in chicken. The body weight was reduced to 14.7% on the inoculated group at 4 weeks post inoculation.     Key Words: Broiler, Local Reovirus Isolate, Runting and Stunting, Malabsorption
The macroscopic and microscopic patology changes on broiler infected with local reovirus isolate Wahyuwardani, Sutiastuti; Huminto, Hernomoadi; Parede, Lies
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 1 (2005)
Publisher : Indonesian Animal Sciences Society

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Abstract

Local reovirus isolate is the virus which could be isolated from runting and stunting syndrome. The ability of local reovirus isolate to induce runting and stunting syndrome in broilers chicken was investigated. The day old chicks (doc) were infected with local reovirus isolate assessed clinically and pathologically at 1, 2 and 3 weeks post inoculation. A total of 40 DOC were divided into two groups. The first group (20 doc) was orally infected with ± 2 x 103 local reovirus isolate particle and the other used as negative control group. The results showed that the isolate caused wet droppings, stunting, enteritis, pancreatitis, malabsorbtion, bursal atrophy and spleenic hypertrophy, which similar to runting and stunting syndrome (RSS) disease in chicken. The body weight was reduced to 14.7% on the inoculated group at 4 weeks post inoculation.     Key Words: Broiler, Local Reovirus Isolate, Runting and Stunting, Malabsorption
Deteksi Dini Penyakit Tumor Sel Darah Myelosit Leukosis melalui Pemeriksaan Ulas Darah Agungpriyono, Dewi Ratih; Huminto, Hernomoadi; Estuningsih, Sri; Satyaningtijas, Aryani Sismin
Jurnal Ilmu Pertanian Indonesia Vol 12, No 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Original Source | Check in Google Scholar | Full PDF (149.708 KB)

Abstract

Myelocytes blood cell tumor in chicken is a disease caused by retrovirus, avian leukosis virus subgroup J (ALV-J). The virus has the same group as human retrovirus (HIV) which caused AIDS, but the avian type possesses oncogenic properties, that could induce cell transformation and tumor formation. ALV-J stimulates the bone marrows myelocytes and transforms them into tumor cell myelocytoma. The tumor cells then metastasis through the circulatory system causing myeloid leukosis and tumor cells accumulation in various internal organs or myelocytomatosis. This study was done on the base of the leukosis behavior of the tumor. The finding of the metastasis tumor cell in the blood smear examination is thought could be use as the diagnostic clue of the disease. Blood smear from sick chickens are collected and stained with some chemical staining substance such as may grunwald-giemsa, hematoxyllin eosin, periodic acid Schiff, congo red, toluidine blue, and sudan black B. Cytochemistry character of the blood cells was observed using light microscope. The result showed that myelocytes granules were best observed using hematoxyllin eosin, periodic acid Schiff, congo red and toluidine blue while may grunwald-giemsa, and sudan black B could not differentiate the granules. By this method, the field veterinarian will able to screen the suspected chicken flock for myeloid leukosis earlier than the occurrence of tumor formation.
Melacak Antigen Virus Gumboro pada Bursa Fabrisius dan Limpa Menggunakan Metode Elisa (DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS ANTIGEN IN BURSA OF FABRICIUS AND SPLEEN USING ELISA) Mirah Adi, Anak Agung Ayu; Huminto, Hernomoadi; Hernomoadi, Lies Parede
Jurnal Veteriner Vol 2, No 4 (2001)
Publisher : Jurnal Veteriner

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Abstract

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The macroscopic and microscopic patology changes on broiler infected with local reovirus isolate Wahyuwardani, Sutiastuti; Huminto, Hernomoadi; Parede, Lies
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 1 (2005): MARCH 2005
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (337.56 KB)

Abstract

Local reovirus isolate is the virus which could be isolated from runting and stunting syndrome. The ability of local reovirus isolate to induce runting and stunting syndrome in broilers chicken was investigated. The day old chicks (doc) were infected with local reovirus isolate assessed clinically and pathologically at 1, 2 and 3 weeks post inoculation. A total of 40 DOC were divided into two groups. The first group (20 doc) was orally infected with ± 2 x 103 local reovirus isolate particle and the other used as negative control group. The results showed that the isolate caused wet droppings, stunting, enteritis, pancreatitis, malabsorbtion, bursal atrophy and spleenic hypertrophy, which similar to runting and stunting syndrome (RSS) disease in chicken. The body weight was reduced to 14.7% on the inoculated group at 4 weeks post inoculation.     Key Words: Broiler, Local Reovirus Isolate, Runting and Stunting, Malabsorption