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Application of maggot in ornamental fish culture Subamia, I Wayan; Himawan, Yogi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.682 KB)

Abstract

Maggot is one of alternative food source that potential to develop. At present, technique 0f culture of maggot can be done especially using media of Palm Kernel Meal (PKM). Application of maggot conducted in form of live food, freeze, and meal Application of maggot have been done for several commodity of ornamental fish especially in Center of Research and Development ofOrnamental Fish Aquaculture. Research using aquarium in indoor and conicle tank at outdoor.. Aplication maggot to the ornamental fish such as Gurame padang (Osphronemus gouramy), Rainbow Kurumoi (Melanotaenia parva), Botia (Chromobotia macracanthus), and Red cherry shrimp (Neocaridina heteropoda) results average of final weight and total length respectively 241,27 g and 18,26 cm ; 15,23 g and 24,06 cm ; 1,8 g and 4,9 cm ; 0,035 g and 0,03 cm. Meanwhile, survival rate of all ornamental fish in that research achieve 100%. Water quality parameter in that research still in good condition. Base on results of research, maggot give the positive effect to the growth and survival rate off all ornamental fish that tested
Performa Udang Hias Red Cherry (Neocaridina heteropoda) pada Fase Pembesaran Melalui Aplikasi Warna Wadah Berbeda Subamia, I Wayan; Himawan, Yogi
Al-Kauniyah: Jurnal Biologi Vol 7, No 1 (2014): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v7i1.2711

Abstract

Shrimp red cherry shrimp is one of the ornamental potential developed. One of the conservation efforts can be made to increase production by optimizing keragaannya on enlargement phase. This study aims at scaling up the production of red cherry shrimp, especially in the phase of enlargement. Treatment in the form of background color is composed of (A) without colors (control), (B) white, (C) in black, and (D) red. Each treatment was repeated three times with a 45-day long research. Container maintenance in the form of an aquarium measuring 14x14x14 cm3 volume of 1 l and equipped with aeration. The prawns used were 10 fish / aquarium with a weighted average of 0.018 ± 0.21 g and the total length of the average of 0.82 ± 0.21 cm. Feeding in the form of an ad bloodworm satiation and include aquatic plant Hydrilla sp. as a source of additional food and shelter in the form of detritus. The results showed that the weight gain and the highest long background color was achieved by treatment D (red) which is equal to 0.09 ± 0.3 g and 1.62 ± 0.3 cm. Survival at each treatment reached 100%. The water quality of all treatments during the study period remained within normal limits.
PERFORMANCES OF POPULATIONS OF SEX REVERSED TILAPIA AND GENETICALLY MALE TILAPIA IN FINGERLING PONDS Ariyanto, Didik; Listiyowati, Nunuk; Himawan, Yogi
Indonesian Aquaculture Journal Vol 4, No 2 (2009): (December 2009)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (95.354 KB) | DOI: 10.15578/iaj.4.2.2009.79-86

Abstract

One of the most popular techniques in mass production of all or nearly all male tilapia population is through sex reversal. However, cross mating of YY super male with regular brood-stock is gaining popularity and has been practiced by aqua-culturists. This study was conducted to evaluate the performance of 2 populations of sex reversed tilapia (sex reversed with dietary application of 17-α methyl testosterone, SRT-1, and sex reversed with dietary administration of imidazole, SRT-2) and 1 population of genetically male tilapia (GMT) derived from YY super male until the end of fingerling rearing period. Mixed sex tilapia (MST) derived from normal mating was used as the control population. The results showed that the daily growth rates of SRT-1, SRT-2, GMT, and MST were 0.12, 0.11, 0.20, and 0.07 g/day, with survival rates were 57.83%, 64.67%, 39.67%, and 68.78%, respectively. The GMT has higher harvest yield than the other populations; SRT-1 (+15.73%), SRT-2 (+6.37%), and MST (+20.82%).
PERFORMA PEMBESARAN IKAN MAS RAJADANU (Cyprinus carpio) GENERASI KETIGA HASIL SELEKSI “WALKBACK” Himawan, yogi; Syahputra, Khairul; Ariyanto, Didik
Jurnal Riset Akuakultur Vol 12, No 2 (2017): (Juni 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.747 KB) | DOI: 10.15578/jra.12.2.2017.121-129

Abstract

Timbulnya wabah penyakit KHV (Koi herpesvirus) pada budidaya ikan mas telah menurunkan produksi ikan mas nasional secara signifikan. Beberapa langkah dilakukan untuk mengatasi penyakit tersebut, salah satunya pembentukan ikan mas Rajadanu tahan KHV melalui seleksi. Dalam rangka memperoleh varietas unggul ikan mas tahan KHV pada tahun 2014 telah dilakukan pembentukan populasi F-3 melalui program selective breeding dengan metode walkback selection. Tujuan penelitian ini adalah menganalisis performa pertumbuhan ikan mas Rajadanu generasi ketiga hasil seleksi melalui uji skala lapang pada fase pembesaran, serta kegiatan uji tantang pada skala laboratorium. Benih F-3 merupakan hasil pemijahan F-1 betina dan F-2 jantan hasil seleksi positif MHC (Major Histocompatibility Complex), sedangkan kontrol berupa benih ikan mas Majalaya dari pembudidaya, masing-masing tiga ulangan. Uji tantang KHV dilakukan di akuarium berukuran 60 cm x 40 cm x 40 cm di hatchery ikan mas dengan suhu permissive 21°C melalui metode kohabitasi. Hasil penelitian menunjukkan bahwa populasi F-3 ikan mas Rajadanu hasil seleksi memiliki performa pertumbuhan relatif lebih baik 6,20% dibandingkan dengan kontrol pada karakter panjang total (18,14±4,90 cm), 5,80% lebih baik pada karakter panjang standar (14,76 ± 4,39 cm); 38,39% lebih baik pada karakter bobot rata-rata (393,98 ± 3,53 g); 16,83% pada karakter laju pertumbuhan spesifik (2,29 ± 0,04%); dan 3,80% pada karakter sintasan (49,13 ± 6,88%). Hasil uji tantang KHV menunjukkan sintasan populasi F-3 lebih tinggi (96,6 ± 3,3%) dibandingkan dengan kontrol (51,1 ± 8,3%).The outbreaks of KHV (Koi herpesvirus) disease in carp culture have significantly reduced Indonesia’s national carp production. Several strategies have been devised to combat the outbreaks including the development of KHV resistant carp varieties such as selective carp breeding program in 2014. The purpose of this study was to analyze the growth performance of the third generation (F-3) Rajadanu through field-scale test (grow out) and challenge test (laboratory-scale). The study was designed in a completely randomized design with two treatments (F-3 populations) and carp seed from the local small-scale fish farmers as control, each with three replicates. F-3 seed was produced from spawning between F-1 females and F-2 males which had a Major Histocompatibility Complex positive selection. The challenge test was carried out in the aquarium sized 60 cm x 40 cm x 40 cm at the hatchery with the permissive temperature of 21°C. The results showed that F-3 Rajadanu population had relatively higher total length (18.14 ± 4.90 cm) of 6.20%, standard length (14.76 ± 4.39 cm) of 5.80%, weight average (393.98 ± 3.53 g) of 38.39%, specific growth rate (2.29 ± 0.04%) of 16.83%, and survival rate (49.13 ± 6.88%) of 3.80% than that of control population. The result of the challenge test on KHV in the laboratory showed that F-3 population had higher survival rate (96.6 ± 3.3%) compared to that of control (51.1 ± 8.3%).
PEWARISAN MARKA Cyca-DAB1*05 DAN KERAGAMAN GENETIK IKAN MAS (Cyprinus carpio) STRAIN RAJADANU TAHAN INFEKSI KOI HERPESVIRUS DAN TUMBUH CEPAT Syahputra, Khairul; Himawan, Yogi; Ariyanto, Didik; S. Palim, Flandrianto
Jurnal Riset Akuakultur Vol 11, No 2 (2016): (Juni 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.382 KB) | DOI: 10.15578/jra.11.2.2016.115-123

Abstract

Balai Penelitian Pemuliaan Ikan (BPPI) telah membentuk ikan mas Rajadanu tahan infeksi koi herpesvirus (KHV) dan tumbuh cepat melalui program seleksi. Ikan mas Rajadanu dihasilkan dari kegiatan seleksi bersamaan pada karakter ketahanan terhadap KHV dan pertumbuhan. Seleksi karakter ketahanan terhadap KHV dilakukan dengan menggunakan marka Cyca-DAB1*05 dan seleksi karakter pertumbuhan cepat dilakukan dengan metode seleksi individu. Penelitian ini bertujuan untuk mengevaluasi pewarisan marka Cyca-DAB1*05 dan keragaman genotipe ikan mas varietas Rajadanu tahan infeksi KHV dan tumbuh cepat generasi ketiga (F3). Sebanyak 44 individu ikan mas Rajadanu F3 digunakan pada penelitian ini. Evaluasi marka Cyca-DAB1*05 dilakukan dengan metode PCR menggunakan primer spesifik marka Cyca-DAB1*05. Analisis keragaman genotipe dilakukan menggunakan tiga lokus mikrosatelit (MFW6, MFW7, dan MFW9). Data alel mikrosatelit dianalisis menggunakan program Fstat dan Arlequin. Hasil penelitian menunjukkan bahwa semua individu pada populasi ikan mas Rajadanu F3 membawa marka Cyca-DAB1*05. Ikan mas Rajadanu F3 memiliki keragaman genotipe yang relatif rendah. Nilai rata-rata heterozigositas teramati (0,31) lebih kecil daripada rata-rata heterozigositas harapan (0,46). Nilai positif pada indeks fiksasi (0,32) menunjukkan status inbreeding pada populasi tersebut. Hasil penelitian ini dapat menjadi acuan, baik dalam kegiatan manajemen induk maupun dalam program seleksi selanjutnya dengan tujuan untuk mempertahankan keragaman genetik dan meminimasi tekanan inbreeding ikan mas Rajadanu tahan infeksi KHV dan tumbuh cepat.Research Institute for Fish Breeding (RIFB) has created Rajadanu common carp resistant to koi herpesvirus (KHV) infection and fast growth through selection program. Rajadanu common carp has been produced by independent culling selection on resistant to KHV and fast growth characters. Selection on resistant to KHV and fast growth character was conducted using Cyca-DAB1*05 marker and mass-selection method respectively. The aim of this study was to evaluate the inheritance of Cyca-DAB1*05 as a marker and the genotype variation of Rajadanu common carp resistant to KHV infection and fast growth. A total of 44 fish were used for this study. Evaluation of Cyca-DAB1*05 marker was conducted by PCR method using specific primer to Cyca-DAB1*05 marker. Analysis of genotype variation was conducted using three microsatellite loci (MFW6, MFW7, and MFW9). Microsatellite allele data was analyzed using Fstat and Arlequin software. The result showed that all of F3 Rajadanu common carp were positive carrying Cyca-DAB1*05 marker. Genetic variation of this population was relatively low. The average observed heterozygosity (Ho=0.31) was lower than the average expected heterozygosity (He=0.46). Positive value of fixation index (0.32) showed inbreeding status in this population. The result of this study can be use as reference for broodstock management and next selection program to maintain the genetic diversity and to minimize inbreeding depression level of Rajadanu common carp resistant to KHV infection and fast growth.
Performa ikan hias rainbow kurumoi (Melanotaenia parva Allen, 1990) dengan pemberian maggot [Performance of ornamental fish Lake Kurumoi rainbowfish (Melanotaenia parva Allen, 1990) fed using maggot] Himawan, Yogi; Subamia, I Wayan
Jurnal Iktiologi Indonesia Vol 13, No 2 (2013): Desember 2013
Publisher : Masyarakat Iktiologi Indonesia (MII )

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.587 KB) | DOI: 10.32491/jii.v13i2.102

Abstract

Kurumoi rainbowfish (Melanotaenia parva Allen, 1990) is an Indonesian ornamental fish which has quite attractive color. The objective of eksperiment is to determine the performance of rainbow kurumoi fed using maggot. Treatment design used completely randomized design (CRD), in the form of feeding: A (pellet), B (maggot), and C (maggot fish silage). Each treatment was repeated three times. Rainbow Kurumoi that used have an average weight of 9.1±0.1 g and standard length of an average of 3.5±0.02 cm, maintained solids tocking 10 fish per container in containers measuring length x width x height is 50 cm x 40 cm x 30 cm and filled with water as much as ±20 L with a stagnant system for 21 days. Feeding as much as 10% of the biomass was given twice a day. The results showed that the highest growth achieved in treatment C with final weights and average length of raw 15.23±0.5 g and 5.2±1.0 cm. Survival rate of each treatment had similar results which amounted to100%. Water quality parameters during the maintenance period are still in the normal range. AbstrakIkan hias rainbow kurumoi (Melanotaenia parva Allen, 1990) merupakan spesies asli Indonesia yang memiliki warna cukup menarik. Tujuan penelitian ini adalah untuk menganalisis performa ikan hias rainbow kurumoi yang diberi pa-kan maggot. Perlakuan menggunakan Rancangan Acak Lengkap (RAL), berupa pemberian pakan: A (pelet), B (maggot), dan C (maggot silase ikan). Tiap perlakuan diulang tiga kali. Ikan rainbow kurumoi dengan bobot rata-rata 9,1±0,1 gram dan panjang baku rata-rata 3,5±0,02 cm, dipelihara dengan padat tebar 10 ekor per wadah dalam wadah berukuran panjang x lebar x tinggi adalah 50 cm x 40 cm x 30 cm dan diisi air sebanyak ± 20 L dengan sistem stagnan selama 21 hari. Pemberian pakan sebanyak 10% dari biomassa dua kali setiap hari. Hasil penelitian menunjukkan bahwa pertum-buhan tertinggi dicapai pada perlakuan C dengan bobot akhir dan panjang baku rata-rata 15,23±0,5 gram dan 5,2±1,0 cm. Sintasan tiap perlakuan memiliki hasil yang sama yakni sebesar 100%. Parameter kualitas air selama masa pemeliharaan masih berada pada kisaran normal.
TRANSMISI GEN krt-GP11 DAN PERFORMA KETAHANAN IKAN MAS (Cyprinus carpio) TRANSGENIK F-2 TERHADAP INFEKSI KHV Syahputra, Khairul; Palimirmo, Flandrianto Sih; Himawan, Yogi
Jurnal Riset Akuakultur Vol 11, No 4 (2016): (Desember 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (508.721 KB) | DOI: 10.15578/jra.11.4.2016.297-305

Abstract

Pembentukan ikan mas transgenik merupakan salah satu program penelitian di Balai Penelitian Pemuliaan Ikan, Sukamandi dalam rangka menghasilkan varietas unggul ikan mas tahan infeksi KHV (Koi herpesvirus). Pada tahun 2015 telah dilakukan pembentukan ikan mas transgenik tahan KHV generasi F-2. Penelitian ini bertujuan untuk mengevaluasi transmisi gen krt-GP11, ketahanan ikan mas transgenik F-2 terhadap infeksi KHV, keberadaan marka Cyca-DAB1*05 tahan KHV pada populasi ikan mas transgenik F-2. Ikan mas transgenik F-2 dihasilkan dengan memijahkan ikan mas transgenik F-1 jantan dengan betina non-transgenik. Pengujian transmisi transgen dan deteksi marka ketahanan KHV pada transgenik F-2 dilakukan dengan metode PCR menggunakan primer spesifik untuk transgen krt-GP11 dan gen Cyca-DAB1*05. Evaluasi ketahanan ikan mas transgenik F-2 terhadap infeksi KHV dilakukan dengan uji tantang secara kohabitasi. Hasil penelitian menunjukkan bahwa transmisi gen krt-GP11 pada keturunan F-2 memiliki persentase yang relatif rendah yaitu sebesar 0%-2%. Ikan mas transgenik F-2 memiliki ketahanan relatif baik terhadap KHV dengan sintasan uji tantang sebesar 90% dan tidak berbeda nyata dengan ikan mas pembanding atau non-transgenik (P>0,05). Tingginya pesentase keberadaan marka Cyca-DAB1*05 pada populasi transgenik berperan pada ketahanan ikan mas transgenik terhadap infeksi KHV.Creating of transgenic common carp is one of the breeding programs in Research Institute for Fish Breeding for producing a superior strain of common carp resistant to KHV(Koi herpesvirus). Since 2015, the creation of common carp transgenic has been conducted to produce F2 population resistant to KHV. This study was aimed to evaluate the transmission of krt-GP11 gen,the resistantce of F2 transgenic common carp against to KHV infection, and the existence of Cyca-DAB1*05 marker resistant to KHV in F2 transgenic population. F2 transgenic population has been produced by mating F1 transgenic male with non transgenic female. Transgene transmission and the existence of marker resistant to KHV in F2 transgenic population were evaluated by PCR method using specific primer to krt-GP11 gene and Cyca-DAB1*05 gene, respectively. The resistance of F2 transgenic population againstto KHV infection was evaluated by challenge test using cohabitation method. The result showed that transmission of krt-GP11 gene in F2transgenic population was relatively low with percentage of 0-2%. The resistance of F2 transgenic common carp against to KHV was relatively high with survival rate of 90% and was not significantly different from non transgenic (p>0.05). High percentage of transgenic population having Cyca-DAB1*05 marker had a role in resistance of transgenic population against KHV infection.
PENGARUH GENOTIPE, LINGKUNGAN, DAN INTERAKSI KEDUANYA TERHADAP STABILITAS PENAMPILAN FENOTIPIK IKAN MAS Ariyanto, Didik; Suharyanto, Suharyanto; Palimirmo, Flandrianto Sih; Himawan, Yogi
Jurnal Riset Akuakultur Vol 13, No 4 (2018): (Desember 2018)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.738 KB) | DOI: 10.15578/jra.13.4.2018.289-296

Abstract

Ikan mas (Cyprinus carpio) merupakan komoditas budidaya yang mudah beradaptasi dengan berbagai kondisi lingkungan. Penelitian ini bertujuan mempelajari pengaruh genotipe, lingkungan, dan interaksi antara genotipe dengan lingkungan terhadap stabilitas penampilan fenotipik ikan mas dalam kegiatan budidaya. Rancangan percobaan menggunakan rancangan faktorial 3 x 5 dengan lima ulangan. Lima strain ikan mas, yaitu Rajadanu, Sutisna, Majalaya, Wildan, dan Sinyonya dipelihara secara komunal di dalam tiga model wadah budidaya, yaitu kolam beton, kolam jaring, dan kolam tanah, selama 90 hari. Hasil penelitian menunjukkan bahwa penampilan fenotipik ikan mas dipengaruhi oleh genotipe, lingkungan dan interaksi kedua faktor tersebut. Strain Sutisna dan Wildan mempunyai nilai sintasan yang paling baik di semua lingkungan dibanding tiga strain lainnya. Strain Sutisna mempunyai pertumbuhan terbaik di kolam tanah sedangkan strain Wildan di kolam jaring. Hal ini menyebabkan kedua strain tersebut menghasilkan biomassa panen terbaik pada lingkungan yang berbeda. Hasil analisis stabilitas menunjukkan bahwa kelima strain ikan mas dalam penelitian ini relatif tidak stabil dan mempunyai respons yang berbeda jika dipelihara pada lingkungan yang berbeda. Strain Wildan dan Rajadanu merupakan strain ikan mas yang mempunyai respons terhadap perbedaan lingkungan paling tinggi. Strain dengan karakteristik tersebut akan mempunyai performa terbaik pada lokasi dan kondisi pemeliharan yang sesuai dengan kebutuhannya, tetapi mempunyai penampilan fenotipik yang rendah jika kondisi lingkungan budidayanya tidak sesuai. Strain Sutisna, Sinyonya, dan Majalaya merupakan strain ikan mas dengan daya responsi terhadap lingkungan lebih rendah. Karakteristik ini menyebabkan penampilan fenotipik ketiga strain tersebut relatif stabil pada semua lokasi dan kondisi budidaya, meskipun tidak bisa mencapai hasil yang maksimal.Common carp (Cyprinus carpio) is known as fish species highly adaptable to various environmental conditions. This study aimed to evaluate the effect of genotype, environment, and their interaction in phenotypic performance stability of common carp. The experimental design used a 3 x 5 factorial design with five repetitions. Five strains of common carp, namely Rajadanu, Sutisna, Majalaya, Wildan, and Sinyonya were stocked communally for 90 days in three culture systems: concrete pond, net cage pond, and earthen pond. The result showed that the phenotypic performance of common carp was influenced by genotype, environment, and their interaction. Sutisna and Wildan strains have a higher survival rate compared to other strains in all culture systems. Sutisna and Wildan strains have the best growth performance in the earthen pond and net cage pond, respectively. Both strains also have the highest biomass production at harvest in all culture systems. Based on the stability performance analysis, Wildan and Rajadanu have the highest response to the different environmental conditions. Strains with this characteristic perform best in different locations or culture systems as long as the environmental conditions are suitable. However, these fish will likely perform poor in the unsuitable culture environment. Sutisna, Sinyonya, and Majalaya are carp strains with lower responsiveness to environmental change. Such characteristic causes the phenotypic performance of these three strains cannot achieve the maximum results, yet it is relatively stable in all locations.
TRANSMISI TRANSGEN GLIKOPROTEIN DAN KETAHANAN IKAN MAS (Cyprinus carpio) TRANSGENIK F1 TERHADAP INFEKSI KOI HERPES VIRUS (KHV) Syahputra, Khairul; Himawan, Yogi; Ariyanto, Didik
Jurnal Riset Akuakultur Vol 10, No 2 (2015): (Juni 2015)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (740.918 KB) | DOI: 10.15578/jra.10.2.2015.153-160

Abstract

Ketahanan penyakit merupakan salah satu karakter selain pertumbuhan yang potensial dikembangkan dengan metode transgenesis pada ikan budidaya. Penelitian ini bertujuan untuk mengevaluasi transmisi transgen glikoprotein-GP11 (GP11) dari KHV dan menguji ketahanan ikan mas transgenik F1 terhadap infeksi koi herpes virus (KHV). Empat garis keturunan F1 transgenik (B1, B2, SA1, dan SA2) diproduksi dengan menyilangkan ikan mas jantan F0 yang membawa gen GP11 di sperma dengan betina non-transgenik.Pengujian transmisi transgen dilakukan dengan mendeteksi transgen pada larva dan benih transgenik F1. Deteksi transgen dilakukan dengan metode PCR menggunakan primer spesifik untuk konstruksi gen glikoprotein (krt-GP11). Evaluasi ketahanan terhadap KHV dilakukan dengan uji tantang secara kohabitasi. Hasil penelitian menunjukkan bahwa tidak semua jantan F0 mentransmisikan transgen pada generasi F1. Transmisi transgen pada ikan mas transgenik F1 berkisar antara 0%-3%. Ikan mas transgenik F1 lebih tahanterhadap infeksi KHV dibandingkan non-transgenik. Ikan mas transgenik F1 memiliki sintasan (85,56±7,29%) yang lebih baik dibandingkan dengan ikan mas non-transgenik (71,11±18,99%).