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Survival Rate Ikan Bawal Bintang (Trachinotus blochii) dalam Media Pemeliharaan Menggunakan Rekayasa Salinitas Arrokhman, Salim; Abdulgani, Nurlita; Hidayati, Dewi
Jurnal Sains dan Seni ITS Vol 1, No 1 (2012): Jurnal Sains dan Seni ITS (ISSN 2301-928X)
Publisher : Jurnal Sains dan Seni ITS

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Abstract

Penelitian ini bertujuan untuk membandingkan Survival Rate (SR) ikan bawal bintang (Trachinotus blochii) yang dipelihara dengan salinitas berbeda dalam rangka diversifikasi spesies perikanan budidaya air payau. Penelitian ini dilakukan dengan memelihara ikan bawal bintang dalam akuarium berkapasitas 70 L menggunakan 4 perlakuan salinitas yaitu salinitas laut (32‰), 24‰, 14‰, dan 4‰ selama 28 hari. Parameter utama yang diamati adalah survival rate (SR) sedangkan parameter kualitas air yang diamati meliputi salinitas (‰), suhu (oC), oksigen terlarut/DO (mg/L), dan pH. Hasil penelitian menunjukkan bahwa SR tidak berbeda nyata antar perlakuan (P>0,05) berdasarkan uji ANOVA one-way dengan taraf kepercayaan 95% dengan kisaran antara 97,1% hingga 100%. Kualitas air masih dalam kisaran aman yaitu suhu antara 28-30oC, DO antara 4 – 5 mg/L, dan pH antara 7,77 – 8,2. Dari hasil penelitian dapat disimpulkan bahwa Survival rate ikan bawal bintang yang dipelihara pada salinitas yang berbeda selama 28 hari tidak menunjukkan adanya perbedaan sehingga dapat dikatakan bahwa ikan bawal bintang dapat beradaptasi dengan baik di salinitas yang lebih rendah daripada salinitas laut dan berpotensi untuk dibudidayakan pada skala pertambakan bersalinitas rendah dalam rangka diversifikasi spesies budidaya perikanan air payau
An Evaluative Study on Sidoarjo Mud Flow after Phytoremediation Treatment in Milk Fish (Chanos Chanos) Liver Hidayati, Dewi
IPTEK The Journal for Technology and Science Vol 21, No 4 (2010)
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j20882033.v21i4.51

Abstract

A sudden discharge of Sidoarjo mud flow into the water bodies would disturb aquatic biota, the fisheries culture. The aim of this research is to evaluate the availability of phytoremediated water of Sidoarjo mud flow (abbreviated: pwmf) as a milkfish culture media using liver histological approach. After two weeks acclimatization period, the juvenile and adult of milkfish were exposed the pwmf at the sub lethal concentration and an acute static bioassay at 96 hours. Control fishes were reared in the artificial brackish water. Each treatment of pwmf exposure was done in three replicates. After exposure, the liver fishes were removed and histologically processed using paraffin method and HE staining. Histopathology change was assessed semi quantitative scoring. The result of the histological analyses suggested the liver pathological features such as vacuolation of hepatocytes, the loss of sinusoid integrity from mild to severe occurrence and necrosis.
Histology of Mice Skin Tissue Based on in Vivo Evaluation of the Anticancer Extracts of Marine Sponge Aaptos Suberitoides Nurhayati, Awik Puji Dyah; Hidayati, Dewi; Prastiwi, Rarastoeti; Widyarini, Sitarina; Sukardiman, Sukardiman; Pujitono, Wijayanti
IPTEK The Journal for Technology and Science Vol 22, No 1 (2011)
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j20882033.v22i1.88

Abstract

The sponge Aaptos suberitoides can produce high secondary metabolite with some farmocological activities as antimicrobial, antiviral, antiinflamatory, and anticancer agents. The purpose of this research is to find out correlations between activities of the etanol extracts of marine sponges Aaptos suberitoides on the cancer growth of subcutaneous mice (Mus musculus) injected by Benzo(a)piren. For the purpose the mice were divided into six groups, i.e. I, II, III, IV, V, and VI. Each group was treated with carcinogenic inductions by intravenously injecting the Benzo(a)piren concentration of 0.3g/0.2 ml oleum olivarum. After the cancer was appeared at the fifteenth day, the mice were treated by the anticancer extracts of marine sponges Aaptos suberitoides with concentrations of 500 mg/kg BB (Group IV), 1000 mg/kg BB (Group IV), and 1500 mg/kg BB (Group IV). The treatments were orally done each day for two weeks. At the twentieth week, subcutaneous cancer tissues were taken to make histological preparates using a parafin method. Result of the histological observation indicates that cancer in the mice was fibrosarcoma characterized by the thickening dermis layers, necrosis, mitosis, and nuclear polymorphsm. Necrosis, mitosis, and nuclear polymorphism occurred in Groups II, IV, V, and VI, and did not in Groups I and II. Presentation of necrosis was 20-60%, mitosis was in 3-4 cells, and nuclear polymorphism was 100%. Result of the statistical analyses by using the Kruskal-Wallis method and the Pair Comparison test indicates that the anticancer extracts of marine sponges with the concentrations of 500 mg/kg BB, 1000 mg/kg BB, and 1500 mg/kg BB had no activity inducing mice skin cancer.
Segmentasi Distributor Wilayah Temanggung Menggunakan Analisis Pareto dan Supplier Preferences : (Studi Kasus Pada Perusahaan Pia King Di Temanggung) Hidayati, Dewi; Sirine, Hani
AKSES Vol 3, No 6 (2008): AKSES : Jurnal Ekonomi dan Bisnis
Publisher : AKSES

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Abstract

This studies actually analyze distributor segmentation in Temanggung district by two types analysis such as Pareto and Supplier Preferences in Pia King Company Temanggung. The related study analyzed by Pareto Analysis and Supplier Preferences Analysis. Based on the  result of analysis can take managerial decision. Thus through both of them the managerial decision is made.Keywords : Distributor segmentation, pareto analyis, supplier preferences analysis, and decision managerial
The Aquaeous Extract of Root Nodules Vigna radiata (rnVr) which Inoculated by Rhizobium as an Orally Available Anemia Therapeutic Candidate Hidayati, Dewi; Nurhidayati, Tutik; Hartanto, Shinta; N, Nurjannah
Indonesian Journal of Biotechnology Vol 13, No 2 (2008)
Publisher : Universitas Gadjah Mada

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Abstract

The extract of root nodules Vigna radiata (rnVr) which inoculated by Rhizobium is considered beneficial as an orally available anemia therapeutic candidate, because it contain the leghemoglobin. The positive control mice (group I) were fed with the high nutrient pellet.The twelve mice (Mus musculus) was treated with the “taking rice pellet” that representing the low nutrient food for 21 days until they suffered anemia. Then, the anemia mice were treated orally with rnVr in different concentration groups:II. 0% III.33%; IV.67% and V.100%, respectively and fed with the “aking rice pellet”. After 14 days, the blood mice were collected from orbital sinus. The hemoglobin (Hb) concentration were analyzed by spectrophotometry and blood plasma profile protein were analyzed with electrophoresis (SDS-PAGE). All anemia mice that treated with rnVr showed the increasing of Hb and group that treated with 100% extract of rnVr could reach a normal Hb value, raising from 9.85 to 12.68 g/dL. There were observed the proteins which have molecule weight 36.5 and 35.7 kDa that indicated the existing erythropoietin. The increasing haemoglobin concentration and erythropoietin suggested if extract of rnVr could increasing red blood production and potential as an orally available anemia therapeutic candidate.
Bioaktivitas ekstrak batang Xylocarpus granatum sebagai anti black spot alternatif pada Litopenaeus vannamei pasca panen Kartikasari, Lanny; Nurhayati, Awik Pudji Diah; Setiawan, Edwin; Hidayati, Dewi; Ashuri, Nova Maulidina; Saadah, Noor Nailis; Muzaki, Farid Kamal; Desmawati, Iska
Journal of Tropical Biodiversity and Biotechnology Vol 2, No 1 (2017): June
Publisher : Faculty of Biology, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.16385

Abstract

This research was done to explore bioactivity of Xylocarpus granatum’s stem extract as tyrosinase inhibitor for alternative preventing black spot in Litopenaeus vannamei postharvest. In this research, extraction of bioactive compound from X. granatum stem was done by soaking the stem in 96% methanol for 24h at temperature 29°C. The methanol was evaporated by using rotary evaporator at temperature 50°C. The extract was dilute with distillate water at series of dilutions (10%, 30%, 50%, 70%, 90%, and distillate water as the control), and then immersing L. vannamei into X. granatum’s stem extract as much as 10ml for 6h, and finally the data analyzing use the Kruskal Wallis test and the Least Significant Difference (LSD) test with the degree of confident 95%. The result showed that X. granatum’s stem extract is able to prevent a black spot of L. vannamei after harvesting process. Furthermore, concentration of 10 % of extract is an effective concentration for preventing a black spot and the control has black spot 0.0360 – 0.0373 mm2.
The Aquaeous Extract of Root Nodules Vigna radiata (rnVr) which Inoculated by Rhizobium as an Orally Available Anemia Therapeutic Candidate Hidayati, Dewi; Nurhidayati, Tutik; Hartanto, Shinta; Nurjannah, N.
Indonesian Journal of Biotechnology Vol 13, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.7802

Abstract

The extract of root nodules Vigna radiata (rnVr) which inoculated by Rhizobium is considered beneficial as an orally available anemia therapeutic candidate, because it contain the leghemoglobin. The positive control mice (group I) were fed with the high nutrient pellet.The twelve mice (Mus musculus) was treated with the “taking rice pellet” that representing the low nutrient food for 21 days until they suffered anemia. Then, the anemia mice were treated orally with rnVr in different concentration groups:II. 0% III.33%; IV.67% and V.100%, respectively and fed with the “aking rice pellet”. After 14 days, the blood mice were collected from orbital sinus. The hemoglobin (Hb) concentration were analyzed by spectrophotometry and blood plasma profile protein were analyzed with electrophoresis (SDS-PAGE). All anemia mice that treated with rnVr showed the increasing of Hb and group that treated with 100% extract of rnVr could reach a normal Hb value, raising from 9.85 to 12.68 g/dL. There were observed the proteins which have molecule weight 36.5 and 35.7 kDa that indicated the existing erythropoietin. The increasing haemoglobin concentration and erythropoietin suggested if extract of rnVr could increasing red blood production and potential as an orally available anemia therapeutic candidate.
Prevalensi dan Intensitas Ektoparasit pada Ikan Gabus (Channa striata) dari Tangkapan Alam dan Budidaya Salam, Bahruddin; Hidayati, Dewi
Jurnal Sains dan Seni ITS Vol 6, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (239.896 KB) | DOI: 10.12962/j23373520.v6i1.22802

Abstract

Penelitian ini bertujuan untuk memperoleh data jenis-jenis ektoparasit serta prevalensi (P) dan intensitas (I) ektoparasit pada ikan gabus dari tangkapan alam (TA) dan budidaya (BD). Sampel ektoparasit diambil dari ikan gabus tangkapan alam dan budidaya masing-masing 20 ekor dengan metode scraping (pengerokan) pada bagian kulit dan insang. Sampel ektoparasit ditampung pada cawan Petri dan ditambahkan buffer formalin 0,5%, lalu dipindahkan ke botol plakon berlabel. Selanjutnya diamati menggunakan mikroskop OpticLab, serta dilakukan perhitungan prevalensi dan intensitas. Hasil pengamatan ditemukan 4 jenis ektoparasit, yaitu Oodinium sp. (P TA=75%, P BD=70% ; [I TA=4.4, I BD=8.3] ind. per ikan), Trichodinasp. (P TA=30%, P BD=15%; [I TA=1.3, I BD=1] ind. per ikan), Ichthyophthirius multifiliis (P TA=65%,  P BD=15% ; [I TA=1.61, I BD=1.66] ind. per ikan), Epistylis sp. (P TA=50% ; [I TA=1.6] ind. per ikan). Prevalensi ektoparasit ikan gabus (C. striata) pada tangkapan alam lebih tinggi dibandingkan budidaya. Nilai prevalensi tersebut lebih dipengaruhi faktor abiotik berupa suhu yang menghambat perkembangan fase infektif parasit. Penelitian ini bertujuan untuk memperoleh data jenis-jenis ektoparasit serta prevalensi (P) dan intensitas (I) ektoparasit pada ikan gabus dari tangkapan alam (TA) dan budidaya (BD). Sampel ektoparasit diambil dari ikan gabus tangkapan alam dan budidaya masing-masing 20 ekor dengan metode scraping (pengerokan) pada bagian kulit dan insang. Sampel ektoparasit ditampung pada cawan Petri dan ditambahkan buffer formalin 0,5%, lalu dipindahkan ke botol plakon berlabel. Selanjutnya diamati menggunakan mikroskop OpticLab, serta dilakukan perhitungan prevalensi dan intensitas. Hasil pengamatan ditemukan 4 jenis ektoparasit, yaitu Oodinium sp. (P TA=75%, P BD=70% ; [I TA=4.4, I BD=8.3] ind. per ikan), Trichodinasp. (P TA=30%, P BD=15%; [I TA=1.3, I BD=1] ind. per ikan), Ichthyophthirius multifiliis (P TA=65%, P BD=15% ; [I TA=1.61, I BD=1.66] ind. per ikan), Epistylis sp. (P TA=50% ; [I TA=1.6] ind. per ikan). Prevalensi ektoparasit ikan gabus (C. striata) pada tangkapan alam lebih tinggi dibandingkan budidaya. Nilai prevalensi tersebut lebih dipengaruhi faktor abiotik berupa suhu yang menghambat perkembangan fase infektif parasit.
DIVERSITAS SERANGGA TANAH DI BERBAGAI TIPE TANAH PADA LAHAN REKLAMASI BEKAS TAMBANG KAPUR KABUPATEN TUBAN Oktafitria, Dwi; Hidayati, Dewi; Purnomo, Eko
Florea : Jurnal Biologi dan Pembelajarannya Vol 6, No 1 (2019)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v6i1.4367

Abstract

The purpose of this study was to determine the diversity of soil insect orders in the reclamation area of former limestone quarries in the top soil type, spoil and reject product used in the media for land reclamation processes. This research sampling technique uses a pitfall trap tool. This study used descriptive quantitative data analysis using the Shannon-Wiener diversity index, Simpson dominance index, Pielou species evenness index, and index of Morisista-Horn habitat similarity. Based on the research that has been done it can be concluded that the reclamation land in 2010 with the dominant type of dust has a low diversity category and a high dominance category with the discovery of 4 orders. The reclaimed land in 2014 with the dominant clay type soil has a moderate diversity category and a low dominance category with 7 orders found. Whereas the reclamation land in 2016 with the dominant type of sand has a low diversity category and a high dominance category with the discovery of 8 orders. The spread of species in all three locations was categorized evenly and between locations did not have the same community.
Kolaborasi Institusi Pemerintah – Perguruan Tinggi – LSM dalam Pengelolaan Sampah Plastik Melalui Forum Group Discussion Aunurohim, Aunurohim; Wijayanti, Anies; Hermawan, Hermawan; Zulaika, Enny; Saptarini, Dian; Hidayati, Dewi; Shovitri, Maya; Setiawan, Edwin; Muzaki, Farid Kamal; Desmawati, Iska; Ashuri, Nova Maulidina
SEWAGATI Vol 3, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1276.813 KB) | DOI: 10.12962/j26139960.v3i2.5728

Abstract

Pengelolaan sampah, utamanya plastik, menjadi sorotan Indonesia bahkan dunia terkait dengan pencemaran yang disebabkannya. Pengelolaan sampah plastik menjadi penting untuk dilakukan manakala sudah mencapai taraf yang membahayakan makhluk hidup di perairan ataupun di daratan. Departemen Biologi bersama Dinas Lingkungan Hidup kota Surabaya dan Lembaga Swadaya Masyarakat Komunitas ‘Nol’ Sampah Surabaya mengadakan forum group discussion guna membahas hal tersebut agar diperoleh suatu upaya konkret dalam pengelolaan sampah plastik di Surabaya secara khusus, dan Indonesia secara umum. Hasil forum group discussion kegiatan ini merekomendasikan langkah-langkah konkret berdasarkan prioritas dalam pengelolaan sampah plastik dimulai dari (1) tidak menggunakan sama sekali tas atau kantong plastik, sedotan plastik, dan botol minuman plastik sekali pakai, (2) jika tidak memungkinkan, maka dilakukan pengurangan penggunaan material berbahan dasar plastik sekali pakai, (3) skala prioritas selanjutnya adalah melakukan re-cycle ataupun re-use untuk plastik yang tidak bernilai komersil seperti plastik sachet ataupun bungkus mie agar dapat bernilai fungsional bahkan komersial, (4) dan prioritas terakhir adalah membiasakan membuang sampah terutama plastik pada tempatnya agar mekanisme sortikasi yang dilakukan pemerintah untuk melaksanakan program bebas sampah plastik pada tahun 2025 mendatang dapat terwujud