SRI HENDRASTUTI HIDAYAT
Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor Jalan Kamper, Kampus Dramaga, Bogor 16680

Published : 49 Documents
Articles

Evaluation of Plant Growth Promoting Rhizobacteria as a Protecting Agent Against Cucumber Mosaic Virus and Chilli Veinal Mottle Virus on Chillipepper TAUFIK, MUHAMMAD; HIDAYAT, SRI HENDRASTUTI; SUASTIKA, GEDE; SUMARAW, SIENTJE MANDANG; SUJIPRIHATI, SRIANI
HAYATI Journal of Biosciences Vol 12, No 4 (2005): December 2005
Publisher : Bogor Agricultural University, Indonesia

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Abstract

This study was conducted to evaluate the effectiveness of plant growth promoting rhizobacteria (PGPR) in protecting chillipepper plant from infection of cucumber mosaic virus (CMV) and chilli veinal mottle virus (ChiVMV). Seven isolates of PGPR, i.e. BC1, BTP2H, BTP3G, BTP3O BTP1, BTP2D, and T1F were applied as seed treatment and soil drench. Plants height, number of branch, and fruits weight were measured every one and ten weeks after virus inoculation. Virus concentration in plants and disease incidence were confirmed by enzyme-linked immunosorbant assay (ELISA). Results showed that inoculation with PGPR improved the seed germination. Eight days after sowing, the percentage of PGPR treated seed germination reached 50-84%; whereas those of untreated seed reached only 18%. In general, PGPR treatment significantly reduced (p < 0.05) the effect of virus infection on plant growth. Two PGPR isolates, i.e. BTP1 and BTP2H, maintained fruit weight of infected plants as good as those of healthy plants. Based on ELISA, PGPR was able to inhibit the disease incidence. The BTP3O and BTP2D isolates even protected the plant from ChiVMV infection. Concentration of salicylic acid and peroxidase were relatively higher on plants treated with PGPR than those without PGPR treatment. This gave an indication that PGPR may act as induction agents for systemic acquired resistance. Therefore, PGPR treatment is a promising strategy to control viral diseases on chillipepper.
Detection and Host Range Study of Virus Associated with Pepper Yellow Leaf Curl Disease SULANDARI, SRI; SUSENO, RUSMILAH; HIDAYAT, SRI HENDRASTUTI; HARJOSUDARMO, JUMANTO; SOSROMARSONO, SOEMARTONO
HAYATI Journal of Biosciences Vol 13, No 1 (2006): March 2006
Publisher : Bogor Agricultural University, Indonesia

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Abstract

High incidence of Pepper yellow leaf curl virus (PepYLCV) was observed in Indonesia since early 2000. Disease incidence in Yogyakarta, Central and West Java reached 100% on Capsicum frutescens, but only 10-35% on C. annuum. As an exception, the disease incidence on C. annuum cv. TM 999 was in the range of 70-100%. The causal agent of the disease, PepYLCV, was detected by polymerase chain reaction. Viral specific DNA fragment of the size ~1600 bp and ~550 bp was amplified from infected plants using two pairs of geminivirus universal primers pAL1v1978/pAL1c715, and pAv494/pAc1048, respectively. The PepYLCV has an intermediate host range including plants belonging to the family of Solanaceae, Leguminosae, and Compositae. The species belonging to the families of Cucurbitaceae, Malvaceae, Chenopodiaceae, and Amaranthaceae were resistant to the virus. Physalis floridana, is very prospective as a propagation host for the geminivirus infecting pepper. Nicotiana spp., cucumber, watermelon, cotton, and Sida sp. could be used as a differential host. Besides, Capsicum frutescens cv. Cakra, tomato, N. benthamiana, N. glutinosa, and Ageratum conyzoides could be used as indicator plants for the geminivirus infecting pepper. Key words: Pepper yellow leaf curl virus, disease incidence, host range
Cucumber Mosaic Virus and Chili Veinal Mottle Virus Infection on Growth and Yield Component of Chilli SUBEKTI, DWI; HIDAYAT, SRI HENDRASTUTI; NURHAYATI, ENDANG; SUJIPRIHATI, SRIANI
HAYATI Journal of Biosciences Vol 13, No 2 (2006): June 2006
Publisher : Bogor Agricultural University, Indonesia

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Abstract

A research was undergone to study the effect of single and double infection of Cucumber Mosaic Virus (CMV) and Chili Veinal Mottle Virus (ChiVMV) on the growth and yield of five chilli cultivars, i.e. Prabu, Taro, Jatilaba, Laris, and Keriting Bogor. Mechanical inoculation was conducted to transmit the virus. Infection of the virus was then confirmed with DAS-ELISA. Severe symptom was observed on plant given double infection compared to those given single infection. The rate of plant growth and the amount and weight of fruits were reduced. The type of interaction between CMV and ChiVMV on most chilli cultivar can be considered as interference and additive. Synergism interaction was only observed on cultivar Laris. Based on symptom expression and reduction on yield, it can be concluded that all chilli cultivars used in this study could not hold up the virus infection. Key words: CMV, ChiVMV, interaction, additive, interference, synergism
Response of Various Tomato Genotypes to Begomovirus Infection and Its Improved Diagnostic AIDAWATI, NOOR; HIDAYAT, SRI HENDRASTUTI; HIDAYAT, PURNAMA; SUSENO, RUSMILAH; SUJIPRIHATI, SRIANI
HAYATI Journal of Biosciences Vol 14, No 3 (2007): September 2007
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Begomovirus infection was identified from tomato growing areas in West Java (Bogor), Central Java (Boyolali), and D.I. Yogyakarta (Kaliurang). Efforts to reduce the infection among others are planting resistance varieties. This research was undertaken to evaluate 14 tomato genotypes for their response to the infection. Dot blot hybridization using nonradioactive (digoxigenin) DNA probe was employed to determine the presence of begomovirus in inoculated plants. Polymerase chain reaction-amplified product of DNA clone of tobacco leaf curl virus –Indonesia was used as a source of DNA probe. All of tomato genotypes evaluated in this study was infected separately by three strain of begomovirus (GVPSlm, GVABy, GVCBgr). Tomato genotypes Bonanza, Jelita, Safira, Permata, Presto, PSPT 8, PSPT 5B, Apel-Belgia, Karibia, Mitra, PSPT 9, Marta, and PSPT 2, showed susceptible or highly susceptible response to the three strains of begomovirus. Exception to those was shown by cv. Intan which resulted in moderate resistance when inoculated with GVCBgr although it resulted susceptible response with the other two strains. Dot-blot hybridization technique was proved to be a powerful tool to detect begomovirus infection in plants showing symptom as well as symptom-less plants. Accumulation of the virus in those plants was relatively high, except in cv. Bonanza and Apel-Belgia. Dot-blot hybridization technique using DIG-labeled DNA probe was able to detect begomovirus DNA in infected tissue up to 10-2 dilution factor. Key words: Geminivirus, hybridization, resistance, tomato
Pengaruh Perlakuan Ethyl Methane Sulfonate pada Tanaman Cabai (Capsicum annuum L.) dan Ketahanannya Terhadap Chilli Veinal Mottle Virus (ChiVMV) Manzila, Ifa; Hidayat, Sri Hendrastuti; Mariska, Ika; Sujiprihati, Sriani
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 3 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Ethyl Methane Sulfonate (EMS) may induce mutation leading to somaclonal variation if it is used at the appropriate combination of EMS concentration and exposure time. Variation in somaclonal might be valuable as a source of resistance to plant pathogens including  plant viruses. This study was aimed 1) to determine the optimum EMS concentration and incubation time that may induce somaclonal variation in chilli pepper; and 2) to evaluate the resistance of the somaclone to ChiVMV  infection. Shoot-tip explants of five chilli pepper genotypes (Jatilaba, ICPN 12 no. 4, PBC495, Helem, and Gelora) were treated with EMS at combination of different concentrations (0.25%, 0.5%  1.0% and control),  and incubation time (15, 30, 60 min). Subsequently, each explant was grown  in multiplication media (MS media + 5 mg L-1 BAP + 0.5 mg L-1 TDZ), rooting media (MS  media +  1 mg L-1 NAA), and acclimatization media (mixture of soil : sand : compost  2:1:1 w/w). Our results showed that the higher EMS concentration and the longer incubation period the smaller the number of survive explants. The highest survival rate  20.4 %  was achieved with 0.5% EMS in combination with 60 min  incubation period. This treatment combination also showed induction of phenotypic variation. Two somaclonal plants derived from Gelora genotype, designated as somaclones K1 and K2,  survived until fruit development and maturation. A total of 245 progenies of K1 and 243 progenies of K2, respectively were evaluated for their resistance to ChiVMV infection through mechanical inoculation using ChiVMV-Cikabayan isolate. Following the detection of ChiVMV using DAS-ELISA, it was confirmed that  4.09% of the somaclonal progenies were  resistance to ChiVMV.   Keywords:  Capsicum annuum, ChiVMV, ethyl methane sulfonate, induce mutation, resistance
Diallel Analysis of Chili (Capsicum annuum L.) Resistance to Phytophthora capsici Leonian Yunianti, Rahmi; Sastrosumarjo, Sarsidi; Sujiprihat, Sriani; Surahman, Memen; Hidayat, Sri Hendrastuti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 39, No 3 (2011): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

 To accomplish the study of genetic parameters of chili resistance to P. capsici, thirty cross combinations from six parents’ crosses were made by full diallel method.  The resistance was assessed based on the incidence of disease, following the inoculation of 28 days-old plant of chili.  Inoculation was done by dropping 5 mL of inoculum (105 zoospore mL-1) at the base part of each plant. There was non-allelic interaction and overdominance effect. Chili resistance to P. capsici controlled by   one   positive   gene.  The  parents   contained   more  dominant  gene, with dominance order were IPB-C4, IPB-C10, IPB-C15,  IPB-C9,  IPB-C8,  and  IPB-C2. The prediction of the possible limits of selection if homozygote genes assemble on one individual was 0.676-0.691. Broad-sense and narrow-sense heritability values for the traits were high.    Keywords: chili, diallel analysis, genetic parameter, Phytophthora capsici  
Ketahanan Tiga Genotipe Cabai terhadap Infeksi Dua Isolat Chilli Veinal Mottle Potyvirus Opriana, Endang; Hidayat, Sri Hendrastuti; Sujiprihati, Sriani
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 1 (2012): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Many factors influence virus replication and movement in the plant cell, among others are virulence of the virus and resistance of the infected plants. Infection of two ChiVMV isolates on three chilli pepper genotypes were assayed to observe disease development. The study was conducted in Cikabayan screen house and Laboratory of Plant Virology, Bogor Agricultural University from April to September 2008. Three genotypes of chilli pepper with different resistance to ChiVMV were selected for this study, i.e. IPB C521(highly resistant), IPB C17 (resistant), and IPB C99 (highly susceptible). Each of the genotypes was mechanically inoculated separately with virulent isolate (ChiVMV CKB) and mild isolate (ChiVMV BL) of the virus. Disease incidence and incubation period of the virus were observed based on symptom development, whereas virus titer and translocation were detected using dot immunobinding assay (DIBA). Disease incidence caused by infection of ChiVMV CKB and ChiVMV BL reached 100% on genotype IPB C99, but no disease incidence was observed on genotype IPB C521. Incubation period of ChiVMV CKB was relatively shorter (7 to 9 days) than those of ChiVMV BL (9 to 14 days). Positive reaction on DIBA was strongly shown for ChiVMV CKB with a high score of virus titer (5 to 6) and sensitivity up to dilution factor of 1 : 106. This study shows that ChiVMV CKB has the ability to cause more severe infection on chilli pepper than ChiVMV BL. Keywords: dot immunobinding assay, pepper genotype, ChiVMV
Kriteria Seleksi untuk Perakitan Varietas Cabai Tahan Phytophthora capsici Leonian Yunianti, Rahmi; Sastrosumarjo, Sarsidi; Sujiprihati, Sriani; Surahman, Memen; Hidayat, Sri Hendrastuti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 2 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

&lt;!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:IN; mso-no-proof:yes;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --&gt; Selection criteria for developing Phytophthora blight-resistant varieties of pepper had been done in both laboratory and fi&nbsp; eld conditions. Resistance screening were conducted on 28-days-old pepper plants grown in 72-cell fl&nbsp; ats by inoculating 5 mL of inoculum (contain 105 zoospore mL-1) to the base of each plant. Phytophthora capsici isolate used in this experiment was TG01, which then identifi&nbsp; ed as race-3. The evaluation of pepper genotypes characteristics were conducted in fi&nbsp; eld.&nbsp; Eight characters demonstrated a wide genetic variability; those characters were resistance to Phytophthora blight, infection period, dicotomous height, canopy width, fruit weight, fruit length, fruit diameter and production. These characters along with fruit wall thickness, chlorophyll-a, chlorophyll-b, total chlorophyll had high heritability values. Using path analysis infection period, dicotomous height and fruit diameter are recommended to be simultaneously used as selection criteria for developing Phytophthora blight resistant pepper varieties. Keywords : capsicum, Phytophthora capsici, selection criteria, path analysis
Daya Gabung dan Heterosis Ketahanan Pepaya (Carica papaya L) terhadap Penyakit Antraknosa*) Hafsah, Siti; astrosumarjo, Sarsidi S; Sujiprihati, Sriani; Sobir, ,; Hidayat, Sri Hendrastuti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 3 (2007): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

This study was conducted to determine the relative importance of general (GCA) and specific (SCA) combining ability and heterosis effects on resistance to C. gloeosporioides in selected papaya genotypes. A half diallel mating scheme of five genotypes of papaya were evaluated in the field. The combining ability analysis revealed that both the additive and nonadditive gene effects were present.  Crosses between the resistance and susceptible genotypes showed intermediate disease reaction to papaya anthracnose disease (PAD) suggesting a polygenic system of resistance to the disease. Highly resistance heterosis of 51.51% at Tajur and 48.71% at Gunung Geulis were expressed in crosses between IPB5 x PB000174.   Key words: Papaya, anthracnose, GCA, SCA, heterosis
Infeksi Papaya ringspot virus pada Tanaman Pepaya di Provinsi Nanggroe Aceh Darussalam Hidayat, Sri Hendrastuti; Nurulita, Sari; Wiyono, Suryo
Jurnal Fitopatologi Indonesia Vol 8, No 6 (2012)
Publisher : Jurnal Fitopatologi Indonesia

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Abstract

Severe mosaic symptoms was observed on papaya plants in Meusa village, Kutablang sub district, Bireun district and Lambaro Teunom village, Lembah Seulawah sub district, Aceh Besar district. Systemic mosaic was found in leaves, stem, twig, and fruit. Reverse transcription-polymerase chain reaction (RT-PCR) was conducted to detect the virus causing mosaic symptoms using universal primer for Potyvirus. Amplification of 320 bp DNA fragment was successfully obtained from leaves and fruits bearing severe mosaic symptoms. Nucleotide sequencing was proceeded using the DNA amplicon as the template. Further sequence analysis indicated that Potyvirus isolates from papaya in Aceh show the highest homology (92.7% to 94.7%) with Papaya ringspot virus (PRSV) from Philippines, Thailand, and Indonesia. This is the first report on PRSV infection on papaya in Nanggroe Aceh Darussalam province.   Key words: Potyvirus, RT-PCR, sequencing