YOSHITAKE HAYASHI
Center for Infectious Diseases, Kobe University Graduate School of Medicine, Hyogo 650-0017, Japan

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Analyses of Precore and Core Promoter Mutations of Hepatitis B Virus in Patients with Chronic Hepatitis B in Surabaya, Indonesia JUNIASTUTI, .; AKSONO, EDUARDUS BIMO; UTSUMI, TAKAKO; YANO, YOSHIHIKO; SOETJIPTO, .; HAYASHI, YOSHITAKE; HOTTA, HAK; RANTAM, FEDIK ABDUL; KUSUMOBROTO, HERNOMO ONTOSENO; LUSIDA, MARIA INGE
Microbiology Indonesia Vol 4, No 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

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Abstract

Mutations of precore (A1896) and core promoter (T1762/A1764) of hepatitis B virus can reduce HBeAg production. These mutations are frequently found in the late HBeAg seroconversion. However, it has been a controversy about the role played by precore and core promoter mutations in determining outcome of chronic hepatitis B. In the present study, the variability of precore and core promoter of hepatitis B virus were analyzed using PCR amplification and sequencing, according to the outcome (viral load and HBeAg/anti-HBe) in chronic hepatitis B patients in Surabaya. The study groups included 5 patients with uncomplicated chronic hepatitis B and 10 patients with chronic hepatitis B and liver cirrhosis in Dr. Soetomo Hospital, Surabaya. The control group included 6 blood donors obtained from Indonesia Red Cross, Surabaya. All groups were HBsAg positive. Precore mutation A1896 was predominant in all groups (60%-67% of each), together with precore variant T1858. As reported, precore variant T1858 is a prerequisite for precore A1896 and characteristic for viral genotype. Nevertheless, core promoter mutations T1762/A1764 were predominant only in LC patients (60%). All of these mutations were found mostly after HBeAg seroconversion (anti-HBe+). Of most samples with anti-HBe+, precore mutation was related with low viral load (<105 copies/mL), but core promoter mutations with high viral load (>105 copies/mL). Precore mutation A1896 was predominant in all groups, but core promoter mutations T1762/A1764 were only predominant in LC patients. The precore mutation alone is possible not critical to indicate a poor outcome, the core promoter mutations must be considered also.
High Prevalence of Occult Hepatitis B Infection (OBI) and its Molecular Characteristics among Pregnant Women in Surabaya, Indonesia MEILANI, MEILANI; UTSUMI, TAKAKO; JUNIASTUTI, JUNIASTUTI; AMIN, MOCHAMAD; SOETJIPTO, SOETJIPTO; HAYASHI, YOSHITAKE; LUSIDA, MARIA INGE
Microbiology Indonesia Vol 10, No 1 (2016): March 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.10.1.1

Abstract

Perinatal transmission is the predominant mode of hepatitis B virus (HBV) transmission in countries where HBV infection is endemic. Newborns of HBV infected mothers have a high risk (up to 90%) of chronicity through perinatal transmission. HBsAg serology screening has been recommended to pregnant women, to prevent perinatal HBV infection. However, at present HBV DNA can be detected in serum with negative HBsAg (OBI - occult hepatitis B infection). The aim of this study was to determine the prevalence of occult hepatitis B infection (OBI) in pregnant women in Surabaya, Indonesia and its virological characteristics. A total of 50 HBsAg-negative and anti-HBc-positive sera were tested for anti-HBs and HBeAg. HBV DNA was isolated from these samples, analyzed by polymerase chain reaction (PCR) and sequenced. HBV DNA was detected in 9 (18%) samples, based on part of the S gene sequence. HBV/B3-adw2 was found predominant in 7 (77.7%) samples, HBV/B9-ayw1 in 1 (11.1%) sample, and HBV/C7-adrq+ in 1 (11.1%) sample. Three samples had mutations (Q129H, T131N, M133S, T140I, T126I) in the ‘a’ determinant region, which may play a role in the undetectability of the virus by the common HBsAg detection kit. The prevalence of OBI in pregnant women from Surabaya is high, but still in line with the general population in Asia. Application of anti-HBc antibody or HBV DNA detection in screening would be very beneficial and prevent perinatal transmission from OBI pregnant women.