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PENGARUH MEDIUM PEMISAH, PENAMBAHAN EKSTRAK KOPI SEBELUM PROSES PEMISAHAN SPERMATOZOA PEMBAWA KROMOSOM X DAN Y DAN LAMA PENYIMPANAN TERHADAP KUALITAS SEMEN CAIR KAMBING PERANAKAN ETTAWA Hasbi, -; Sonjaya, H.; Gustina, S.
JURNAL ILMU DAN TEKNOLOGI PETERNAKAN Vol 1, No 2 (2011)
Publisher : Fakultas Peternakan Universitas Hasanuddin

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Abstract

One problem in the application of biotechnology in spermatozoa sexing of X and Y sperm chromosome is a decrease in motility and percentage of live spermatozoa. The decrease could be caused by metabolic process, therefore spare energy obtained from separation medium is decreasing resulting in decrease in motility and percentage of live spermatozoa.  Research was carried out to evaluate the effects of separation medium, addition of coffee extract before sexing of X and Y sperm chromosome, and storage period on the quality of goat fresh semen.  This experiment was performed according to completely randomized design in factorial pattern (2 x 2 x 6). The first factor was the separation medium (A1: 10% and A2: 30%), the second factor was the coffee extract addition (B1: 0 mM and B2: 3 mM), and the third factor was the period of storage(D1: 0 hour, D2: 2 hours, D3: 4 hours, D4: 6 hours, D5: 8 hours and D6: 10 hours). The result showed that the motility of spermatozoa Y in 30% separation medium was higher (P<0.01) than that of spermatozoa X in 10% separation medium and it was higher (P<0.05) with addition of coffee extract compared with no coffee extract addition.  Viability of spermatozoa Y was higher (P<0.05) compared with spermatozoa X and addition of coffee extract not significantly different (P>0.05) compared with no coffee extract addition. The motility and viability of spermatozoa decreased during 10 hours storage. It was concluded that the motility and viability of spermatozoa Y was higher than spermatozoa X. Coffee extract can prevent a decrease of spermatozoa motility during storage. The motility of spermatozoa and the percentage of live spermatozoa decreased during storage.   Key words: Separation medium, Coffee extract, Storage period, Fresh semen, Ettawa cross goat
Empat Pilar Neuro Linguistics Programming Dalam Komunikasi di Dunia Pendidikan Untuk Pembentukan Karakter Peserta Didik Hasbi, -
Journal Polingua : Scientific Journal of Linguistics, Literature and Education Vol 3, No 1 (2014)
Publisher : Politeknik Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.678 KB) | DOI: 10.30630/polingua.v3i1.13

Abstract

Communication skill is a precondition for people to be able to influence others and the most effective tools used forcommunication is language. The ability of educators in using the right words are determining the kind of students they have, and inlong term it will determine the character of the next generation of one nation. For teachers to be able to use the right way ofcommunication they need to consciously use the language which are developed in Neuro Linguistics Programming known as NLP.This paper will discuss four pillars of NLP as well as some techniques in NLP for helping teachers in improving their teaching skillsso that they become powerful educator to build character of the next generation. The four pillars of NLP are outcome, rapport,sensory acuity, and flexibility. The techniques namely are meta model, and reframing.Outcome is way of thinking in communication skill which helps parents to focus on the result instead of what is going on with thestudents. Rapport is a way of minimizing miscommunication among people. Sensory acuity is observing carefully and not makingquick conclusion in communication. Flexibility is the ability to do different things when one does not work. Meta model is an effectiveways of finding out what is really mean by the students. This technique is very effective to find out what is really meant by thestudents. Reframing is one technique to persuade the students to obey their teachers order. Using this techniques helping teachers toforce students is soft way.