Dyah Haryuningtyas
Balai Besar Penelitian Veteriner, Jl. R.E. Martadinata 30, Bogor 16114

Published : 16 Documents
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Methods of Detection Anthelmintic Resistance in Sheep and Goats Haryuningtyas, Dyah; ., Beriajaya
Indonesian Bulletin of Animal and Veterinary Sciences Vol 12, No 2 (2002)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.555 KB) | DOI: 10.14334/wartazoa.v12i2.769

Abstract

Anthelmintic resistance to gastrointestinal nematode of sheep and goats has been reported world wide to all of broad spectrum anthelmintic types currently available, such as benzimidazoles, levamizole and ivermectin. In South-East Asia anthelmintic resistance to small ruminants was reported in Malaysia, Thailand and Philippines. In Indonesia resistances to albendazole have been reported from sheep farm in Bogor. With regard to resistance cases, early detection for anthelmintic resistance on sheeps and goats to spesific types of anthelmintic that commonlly used are required to plan rationale strategic control program. Detection methods for anthelmintic resistance are Fecal Egg Count Reduction Test (FECRT), Egg Hatch Assay (EHA) and Larval Development Assay (LDA). LDA is an in vitro assay to detect anthelmintic resistant in the major of gastrointestinal nematodes on sheep and goats that have just been introduced in Indonesia. This paper explains the comparison of three detection methods and goodness of LDA compared with other two methods.   Key words: Anthelmintic resistance, sheep, goat, Fecal Egg Count Reduction Test (FECRT), Egg Hatch Assay (EHA), LarvalDevelopment Assay (LDA)
Development of Anthelmintic Resistance Detection Methods of Gastrointestinal Nematodes on Livestock Haryuningtyas, Dyah
Indonesian Bulletin of Animal and Veterinary Sciences Vol 18, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.398 KB) | DOI: 10.14334/wartazoa.v18i1.909

Abstract

The intensive usage of anthelmintic in most of farms led to resistances of livestock gastrointestinal nematodes against anthelmintic.  Many  reports  of  resistance  that  increased  every  year  happen  following  the  continuing  helminth  control programmes. The succesful implementation of helminth control programmes that designed to minimize the development of resistance in nematode populations depends on the availability of effective and sensitive method for its detection and monitoring. A variety of in vivo and in vitro tests have been developed for detecting nematode population resistance to the main  anthelmintic groups. This paper will discuss the development of detection method of anthelmintic resistance based on conventional and molecular approach according to their strengths and weakness.   Key words: Anthelmintic resistance, detection method, conventional, molecular, nematode
Mutation detection on isotype-1 β tubulin genes of Haemonchus contortus resistant strain to benzimidazole using single strand conformation polymorphism Haryuningtyas, Dyah
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 3 (2005)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.001 KB) | DOI: 10.14334/jitv.v10i3.444

Abstract

Evidence of anthelmintic resistance of Haemonchus contortus to benzimidazole groups based on Larval development assay (LDA) and Fecal egg count reduction test (FECRT) test has been reported in some areas in Indonesia. Studies on sheep parasite H. contortus have shown that resistance to benzimidazole drugs is correlated with selection for individuals in the population possesing a spesific isotype-1 β tubulin gene. The aim of this study was to determine mutation on central part of isotype-1 β tubulin gene of benzimidazole resistant strain H. contortus using Single Strand Conformation Polymorphism (SSCP). H. contortus worms were isolated from four sheep from two government farms that resistance to benzimidazole have been occurred (SPTD Trijaya, Kuningan, West Java and UPTD Pelayanan Kesehatan Hewan, Bantul, Yogyakarta) and one sheep that susceptible from Cicurug, Bogor, West Java as a kontrol. Resistance status to benzimidazole was reexamined individually with LDA and FECRT before sheep slaughtered. DNA was extracted from female H. contortus worms. A fragment of 520 bp isotype- 1 β tubulin gene was amplified using Polymerase chain reaction (PCR) and then analyze using SSCP. The results showed that there were polymorphism in isotype-1 β tubulin gene among H. contortus susceptible (Cicurug, Bogor, Jawa Barat) and two H. contortus resistant strains from SPTD Trijaya, Kuningan, West Java and UPTD Pelayanan Kesehatan Hewan, Bantul, Yogyakarta. Mutation occurred in the different nucleotide of the two resistant strain.     Key Words: Haemonchus contortus, Benzimidazole, Isotype-1 β tubulin gene, SSCP
Sequence variability of the β Tubulin isotype-1 genes in benzimidazole resistant strains of Haemonchus contortus, a nematode parasite of sheep Haryuningtyas, Dyah; Artama, wayan T; Asmara, Widya
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.925 KB) | DOI: 10.14334/jitv.v11i3.531

Abstract

Resistance to benzimidazole at a population of gastrointestinal nematode, especially Haemonchus contortus is a widespread problem. Therefore, anthelmintic will not effective anymore. Benzimidazole resistance in nematode has been shown to be heritable and defined as an increase in frequency of individual worms able to drug tolerate. In Indonesia resistance to anthelmintic albendazole has been reported on sheep in district of Bogor, Kuningan, Kendal and province of Yogyakarta. Molecular mechanism of resistance to benzimidazole on nematode and fungi related to single gene which code ß tubulin. The aim of this research is to determine a genetics variability of isotype-1 ß tubulin genes from Indonesian strain of H. contortus which resistant to benzimidazole. Sequencing were carried out at a 520 bp fragment of the isotipe-1 ß tubulin gene of six H. contortus resistant strain from four sheep from SPTD Trijaya farm, district of Kuningan, West Java; UPTD Pelayanan Kesehatan Hewan farm, district of Bantul, Yogyakarta respectively. One H. contortus susceptible strains as a control come from farmer from Cicurug, district of Sukabumi, West Java. Results indicated that there are genetics variability of  fragment isotype-1 ß tubulin genes from H. contortus from UPTD Pelayanan Kesehatan Hewan farm, Bantul, Yogyakarta (H1, H2, and C2). There are no genetics variability in the fragment gene of H. contortus resistant strain from SPTD Trijaya farm, Kuningan, West Java (Kn1, Kn2 and K1). Genetic variability occur between 2 isolate from different location. Key Words: Haemonchus contortus, Benzimidazole, Isotype 1 β-tubulin Gene, Sequencing
Detection of Brugia malayi microfilaria/Larvae in mosquito using Polimerase Chain Reaction. Haryuningtyas, Dyah; Subekti, Didik Tulus
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 3 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.014 KB) | DOI: 10.14334/jitv.v13i3.587

Abstract

Lymphathic filariasis that is also known as elepanthiasis is caused by infestation of 3 species nematode Wuchereria bancrofti, Brugia malayi and Brugia timori. In Indonesia 70% filariasis case caused by Brugia malayi. Mosquito species from genus Anopheles, Aedes, Culex, Mansonia and Armigeres are known as vector of this disease. Microfilaria detection on mosquito is one methode to know infection rate in vector population in endemic area.The objectives of the research were to study the ability of Hha1 repeat applicable to detect microfilaria/larvae in a pool of mosquitoes and to get description of adult mosquito night biting population lived in endemic area of filariasis brugian. Mosquito as positive control used in this research come from laboratory of parasitology of FKUI. Mosquito sample from the field was from Binawara and Kolam Kiri villages, South Kalimantan province. Mosquito were trapped then identified by its species. DNA of mosquitoes was extracted and then run by the PCR using Hha 1 repeat primer. Result of the research indicated that adult mosquitoes night biting from Binawara village consist of Culex, Mansonia, Anopheles genus and from Kolam Kiri village only from Mansonia genus. Hha 1 repeat primer is applicable to detect 1 mosquito infected with microfilaria/larvae in a pool of negative mosquitoes. Mosquito samplesfrom the two villages showing negative PCR.   Key Words: Filariasis, Brugia Malayi, Vector, Microfilaria, Filaria Larve, PCR
Development of Anthelmintic Resistance Detection Methods of Gastrointestinal Nematodes on Livestock Haryuningtyas, Dyah
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 18, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.398 KB) | DOI: 10.14334/wartazoa.v18i1.909

Abstract

The intensive usage of anthelmintic in most of farms led to resistances of livestock gastrointestinal nematodes against anthelmintic.  Many  reports  of  resistance  that  increased  every  year  happen  following  the  continuing  helminth  control programmes. The succesful implementation of helminth control programmes that designed to minimize the development of resistance in nematode populations depends on the availability of effective and sensitive method for its detection and monitoring. A variety of in vivo and in vitro tests have been developed for detecting nematode population resistance to the main  anthelmintic groups. This paper will discuss the development of detection method of anthelmintic resistance based on conventional and molecular approach according to their strengths and weakness.   Key words: Anthelmintic resistance, detection method, conventional, molecular, nematode
Analisis Sekuen Gen Tubulin-β Isotipe 1 Cacing Haemonchus contortus Isolat Resisten terhadap Benzimidazole pada Domba di Indonesia Haryuningtyas, Dyah; Artama, Wayan T.
Jurnal AgroBiogen Vol 4, No 2 (2008): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Benzimidazole (BZ)resistance to gastrointestinal nematodes in small ruminants(sheep and goat) has become a significant problem worldwide.Evidences of anthelmintic resistance to albendazole inIndonesia has been reported from some government ownedfarms in West Java, Central Java, and Yogyakarta. Previousstudy on the sheep parasite H. contortus had shown that theBZ resistance was related to selection for individuals in apopulation possesing a spesific β-tubulin isotype 1 gene. Thestudy is aimed to determine mutation on coding region ofcentral part of β-tubulin isotype 1 gene of H. contortus resistantstrain from Indonesia. Seven H. contortus worms wereisolated from four BZ resistant sheep from two governmentfarms (SPTD Trijaya, Kuningan, West Java, and UPTDPelayanan Kesehatan Hewan, Bantul, Yogyakarta), and froma BZ susceptible sheep from Cicurug, Sukabumi, West Java.DNA was extracted individually from female H. contortusworms. A fragment of 520 bp β-tubulin isotype 1 gene exon3, 4, 5 was amplified using the PCR technique and thensequenced. The results showed that a single mutationoccurred in codon 200 (from phenilalanine to tyrosine) hadcaused benzimidazole resistance in H. contortus from SPTDTrijaya, Kuningan, West Java. Mutation in β-tubulin isotype 1gene of H. contortus from UPTD Pelayanan KesehatanHewan, Yogyakarta, occurred in codon 198 (from glutamateto glycine), codon 201 (from cystein to stop codon), andcodon 202 (from isoleucyne to stop codon).
Sequence variability of the β Tubulin isotype-1 genes in benzimidazole resistant strains of Haemonchus contortus, a nematode parasite of sheep Haryuningtyas, Dyah; Artama, wayan T; Asmara, Widya
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 3 (2006): SEPTEMBER 2006
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.925 KB) | DOI: 10.14334/jitv.v11i3.531

Abstract

Resistance to benzimidazole at a population of gastrointestinal nematode, especially Haemonchus contortus is a widespread problem. Therefore, anthelmintic will not effective anymore. Benzimidazole resistance in nematode has been shown to be heritable and defined as an increase in frequency of individual worms able to drug tolerate. In Indonesia resistance to anthelmintic albendazole has been reported on sheep in district of Bogor, Kuningan, Kendal and province of Yogyakarta. Molecular mechanism of resistance to benzimidazole on nematode and fungi related to single gene which code ß tubulin. The aim of this research is to determine a genetics variability of isotype-1 ß tubulin genes from Indonesian strain of H. contortus which resistant to benzimidazole. Sequencing were carried out at a 520 bp fragment of the isotipe-1 ß tubulin gene of six H. contortus resistant strain from four sheep from SPTD Trijaya farm, district of Kuningan, West Java; UPTD Pelayanan Kesehatan Hewan farm, district of Bantul, Yogyakarta respectively. One H. contortus susceptible strains as a control come from farmer from Cicurug, district of Sukabumi, West Java. Results indicated that there are genetics variability of  fragment isotype-1 ß tubulin genes from H. contortus from UPTD Pelayanan Kesehatan Hewan farm, Bantul, Yogyakarta (H1, H2, and C2). There are no genetics variability in the fragment gene of H. contortus resistant strain from SPTD Trijaya farm, Kuningan, West Java (Kn1, Kn2 and K1). Genetic variability occur between 2 isolate from different location. Key Words: Haemonchus contortus, Benzimidazole, Isotype 1 β-tubulin Gene, Sequencing
Mutation detection on isotype-1 β tubulin genes of Haemonchus contortus resistant strain to benzimidazole using single strand conformation polymorphism Haryuningtyas, Dyah
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 3 (2005): SEPTEMBER 2005
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.001 KB) | DOI: 10.14334/jitv.v10i3.444

Abstract

Evidence of anthelmintic resistance of Haemonchus contortus to benzimidazole groups based on Larval development assay (LDA) and Fecal egg count reduction test (FECRT) test has been reported in some areas in Indonesia. Studies on sheep parasite H. contortus have shown that resistance to benzimidazole drugs is correlated with selection for individuals in the population possesing a spesific isotype-1 β tubulin gene. The aim of this study was to determine mutation on central part of isotype-1 β tubulin gene of benzimidazole resistant strain H. contortus using Single Strand Conformation Polymorphism (SSCP). H. contortus worms were isolated from four sheep from two government farms that resistance to benzimidazole have been occurred (SPTD Trijaya, Kuningan, West Java and UPTD Pelayanan Kesehatan Hewan, Bantul, Yogyakarta) and one sheep that susceptible from Cicurug, Bogor, West Java as a kontrol. Resistance status to benzimidazole was reexamined individually with LDA and FECRT before sheep slaughtered. DNA was extracted from female H. contortus worms. A fragment of 520 bp isotype- 1 β tubulin gene was amplified using Polymerase chain reaction (PCR) and then analyze using SSCP. The results showed that there were polymorphism in isotype-1 β tubulin gene among H. contortus susceptible (Cicurug, Bogor, Jawa Barat) and two H. contortus resistant strains from SPTD Trijaya, Kuningan, West Java and UPTD Pelayanan Kesehatan Hewan, Bantul, Yogyakarta. Mutation occurred in the different nucleotide of the two resistant strain.     Key Words: Haemonchus contortus, Benzimidazole, Isotype-1 β tubulin gene, SSCP
Detection of Brugia malayi microfilaria/Larvae in mosquito using Polimerase Chain Reaction. Haryuningtyas, Dyah; Subekti, Didik Tulus
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 3 (2008): SEPTEMBER 2008
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.014 KB) | DOI: 10.14334/jitv.v13i3.587

Abstract

Lymphathic filariasis that is also known as elepanthiasis is caused by infestation of 3 species nematode Wuchereria bancrofti, Brugia malayi and Brugia timori. In Indonesia 70% filariasis case caused by Brugia malayi. Mosquito species from genus Anopheles, Aedes, Culex, Mansonia and Armigeres are known as vector of this disease. Microfilaria detection on mosquito is one methode to know infection rate in vector population in endemic area.The objectives of the research were to study the ability of Hha1 repeat applicable to detect microfilaria/larvae in a pool of mosquitoes and to get description of adult mosquito night biting population lived in endemic area of filariasis brugian. Mosquito as positive control used in this research come from laboratory of parasitology of FKUI. Mosquito sample from the field was from Binawara and Kolam Kiri villages, South Kalimantan province. Mosquito were trapped then identified by its species. DNA of mosquitoes was extracted and then run by the PCR using Hha 1 repeat primer. Result of the research indicated that adult mosquitoes night biting from Binawara village consist of Culex, Mansonia, Anopheles genus and from Kolam Kiri village only from Mansonia genus. Hha 1 repeat primer is applicable to detect 1 mosquito infected with microfilaria/larvae in a pool of negative mosquitoes. Mosquito samplesfrom the two villages showing negative PCR.   Key Words: Filariasis, Brugia Malayi, Vector, Microfilaria, Filaria Larve, PCR