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AKTIVITAS ANTIOKSIDAN DAN ANTIKANKER TURUNAN BENZALASETON Handayani, Sri; Arianingrum, Retno; Haryadi, Winarto
Jurnal Penelitian Saintek Vol 18, No 1 (2013): APRIL 2013
Publisher : Jurnal Penelitian Saintek

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Abstract

Modifikasi struktur vanilin menjadi vanililaseton dan divanililaseton serta uji aktivitasnya sebagai antioksidan dan antikanker telah dilakukan. Modifikasi struktur dilakukan melalui kondensasi aldol silang dalam suasana basa dengan pelarut etanol­ akuades selama 3 jam pada 10°C. Uji aktivitas antioksidan terhadap senyawa hasil sintesis dilakukan dengan metode degradasi deoksiribosa, sedangkan uji aktivitas antikanker dilakukan dengan metode MTT. Hasil uji aktivitas antioksidan menunjukkan bahwa divanililaseton dengan IC50 sebesar 873 ug/ml. lebih aktif dari pada vanililaseton dengan IC50 sebesar 11,765 ug/ml.. Data IC50 untuk aktivitas antikanker terhadap sel kanker HeLa vanilinaseton dan divanilinaseton secara berturut-turut sebesar 51,68 dan 10,26 ug/ml. Hasil tersebut menunjukkan bahwa kedua senyawa tersebut sangat toksik terhadap sel kanker HeLa.
Isolasi dan Identifikasi Senyawa Terpena Hasil Pirolisis Getah Karet Alam (Hevea brasiliensis) Wairata, Johanis; Mastjeh, Sabirin; Haryadi, Winarto
Berkala Ilmiah MIPA Vol 23, No 2 (2013)
Publisher : FMIPA UGM

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Abstract

Isolation and identification of terpenes obtained from the pyrolisis of natural rubber (Hevea brasiliensis) latex have been conducted. The latex was pyrolized under reduced pressure without any solvent. The pyrolysis products contains water, protein and rubber oil. The rubber oil was separated and identified using GC-MS.The results showed that the rubber oil contained 22 compounds. According to literature review, 12 compounds were identified as terpene, including monoterpene, sesquiterpene and diterpene. Monoterpene compounds were isolimonene, l-limonene, P-felandrene, d-limonene and camphene. The identified sesquitterpenes were 2,4a,8,8-tetramethyl-1,1a,4,4a,5,6,7,8-octahydrocyclopropane [d] naphthalene, P-farnasene, a-amorphene, y-amorphene and bisabolene. The analyzed diterpenes were abietadiene and kaurene. The dominant terpene was d-limonene. The d-limonene was isolated using fractional distillation and had the purity of 96.79%.
Potensi Mikroalgae Isolat Asli Indonesia Sebagai Sumber Energi Terbarukan Haryadi, Winarto
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2014
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

Sumber energi tak terbarukan makin menipis dan suatu ketika pasti habis Perlu eksplorasi sumber energi terbarukan. Sumber energi terbarukan tersebuttidak boleh menganggu sumber pangan
Comparison on Isolation Technique of Vaninilin from Vanili Fruit (Vanilla planifolia Andrews Haryadi, Winarto; Handayani, Sri; Arianingrum, Retno
Eksakta: Jurnal Ilmu-Ilmu MIPA VOLUME 11, ISSUE 2, August 2010
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

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Abstract

Isolation of vanilin from vanili fruit by two method; maseration and Soxhlet using ethylacetate, ethanol and chloroform solvents has been investigated. Extraction result was hydrolized using sodium hydroxide by acidification using chloride acid. Filtrate was extracted by using chloroform and the targeted vanilin was separated by evaporation. Putrification was enganged by rechrystallization using aquadest-ethanol. Elucidation to the compound structure was performed by FTIR spectrophotometer, H-NMR, C-NMR, HMQC and HMBC. It is noted that vanilin isolation by soxhletazion procedure was more effective compared to maseration. Ethylacetate was more effective solvent than chloroform and conversely ethanol is inefective for the isolation.   Keywords: vanilin isolation, soxhlet isolation, maseration  
Polimerisasi Eugenol Minyak Daun Cengkeh Hasil Redistilasi, Ekstraksi, dan Fraksinasi Menggunakan Katalis Asam Sulfat Pekat Sudarlin, Sudarlin; Haryadi, Winarto
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 3, No. 1, Mei 2017
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Syarif Hidayatullah State Islamic Uni

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v3i1.4685

Abstract

Polymerization of purified eugenol from clove leaf oil using concentrated sulfuric acid as catalyst has been done in this research. Clove leaf oil was purified by vacuum redistillation, acid-base extraction, and vacuum fractionation to yield eugenol. The eugenol was polymerized with concentrated sulfuric acid in ratio 1: 2. Polyeugenol from eugenol of clove leaf oil resulted from redistillation, extraction, and fractionation is in solid form (yield: 88.14%); from redistillation and extraction is also in solid form (yield: 86.15%); from redistillation clove leaf oil is in gel form (yield: 100.29%). Structure of polyeugenol was determined by infrared spectrophotometer and characterized by thin layer chromatography (TLC). Molecular weight of the product was calculated by viscometry method.DOI: http://dx.doi.org/10.15408/jkv.v0i0.4685
CYTOTOXICITY IDENTIFICATION OF THE SUPERNATANT OF Manihot esculenta Crantz RHIZOME AS RIBOSOME-INACTIVATING PROTEIN TO NORMAL CELL Astuti, Endang; Matsjeh, Sabirin; Haryadi, Winarto; Pranowo, Deni; Mulatsih, Nuning Sri
Indonesian Journal of Chemistry Vol 4, No 2 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.21857

Abstract

The supernatant of Manihot esculenta Crantz rhizome has been used as an alternative remedy of infectious and cancer in Jogjakarta. Cytotoxicity assay showed that  Manihot esculenta Crantz supernatant had cytotoxic effect to cancer cell line, namely Myeloma (LC50 = 180,24 μg/mL) and HeLa (LC50 = 415,55 μg/mL), but have a little cytotoxic effect to SiHa. This research was aimed to identify cytotoxic activity of Manihot esculenta Crantzs supernatant to normal cell, particularly to human mononuclear cell and Vero cell line, and to compare to the cancer cell lines. The result showed that supernatant of M. esculenta had cytotoxic effect to normal mononuclear cell (LC50 = 564,00 μg/mL) and Vero cell line (LC50 = 686,00 μg/mL). The supernatant of M. esculenta had the highest cytotoxic activity to myeloma and relatively toxic to cervix cancer HeLa and normal cell, but less to SiHa.   Keywords: Manihot esculenta Crantz, Ribosome-inactivating Protein (RIP), cytotoxic, normal cell, cancer cell
STUDY OF EPOXIDE DECYCLISATION OF CARYOPHYLENE OXIDE WITH SYNTHETIC ZEOLITE AS CATALYSTS Haryadi, Winarto; Muchalal, M.
Indonesian Journal of Chemistry Vol 4, No 2 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.21860

Abstract

The reaction of epoxide ring opening of caryophillene oxide has been done using zeolite H-Y, H-sodalit, and H-ZSM-5 as catalysts. The reactions were done in two types, there were in dioxane solvent at temperature of 110 oC and without solvent at temperature of 175 oC. The catalyst weight was 10 % from caryophillene oxide weight, and the time of reaction was four hours. The product of reaction was analyzed using GC, FTIR, and GC-MS. The reactions of caryophillene oxide in dioxane solvent with the three kinds of zeolites did not give any targeted product. Whereas, the reactions without solvent gave three main products, there was one compound with one group of secondary hidroxyl (secondary alcohol), and two compounds of ketone from caryophillene. The reaction product of caryophillene oxide obtained without using solvent with the three type of catalysts were then compared. Conversion of three main products produced by H-ZSM-5 catalyst, H-sodalit catalyst and H-Y catalyst were 82.11 %, 54.92 % and 38.53 % respectively. For that reason, the transformation of caryophillene oxide using H-ZSM-5 catalyst was considered to be the best selective product. The alcohol product was resulted from reaction between caryophillene oxide and Bronsted acid, and  the ketone products was resulted from the reaction with Lewis acid in zeolite.   Keywords: Epoxide ring opening, HY, H-sodalit and HZSM-5
PREPARATION OF ACTIVATED CARBON FROM SILK COTTON WOOD AND COCONUT SHELL BY PYROLISIS WITH CERAMIC FURNACE Haryadi, Winarto; Muchalal, Muchalal; Cahyono, Robby Noor
Indonesian Journal of Chemistry Vol 5, No 2 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.21817

Abstract

Preparation of activated carbon from silk cotton wood and coconut shell has been done. Carbon was made by pyrolysis process in the Muchalal furnace with 3000 watt electric power. The electric power was increased gradually from 1000, 2000 and then 3000 watt with interval 2 hours during 7 hours. Carbon was activated in Muchalal furnace with 4000 watt electric power during 2 hours and flowed with nitrogen gas. Product of the activated carbon was compared to standart product with several analysis including the surface area, acetic acid adsorption, iod adsorption and vapour adsorption. The results of analysis showed that surface area for silk cotton wood carbon, coconut shell carbon, and E.Merck product were 288.8072 m2/g, 222.9387 m2/g and 610.5543 m2/g, respectively. Acetic acid adsorption for silk cotton wood carbon, coconut shell carbon, and standart product were 157.391 mg/g, 132.791 mg/g, and 186.911 mg/g, respectively. Iodine adsorption for cotton wood carbon, coconut shell carbon, and standart product were 251.685 mg/g, 207.270 mg/g and 310.905 mg/g, respectively. Vapour adsorption for cotton wood carbon, coconut shell carbon and standart product were 12%, 4%,and 14%., respectively Key words : Activated carbon, pyrolysis, Muchalal furnace
The Optimum Reaction Time, Activation Energy and Frequency Factor of Methyl Ricinoleate Nitration Abdullah, Abdullah; Triyono, Triyono; Trisunaryanti, Wega; Haryadi, Winarto
Indonesian Journal of Chemistry Vol 13, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.21323

Abstract

Determination of the optimum reaction time, activation energy (Ea) and frequency factor (A) of methyl ricinoleate nitration has been done. The nitration was conducted with the mole ratio of methyl ricinoleate to HNO3 of 1:15. The reaction was conducted at temperatures of 29 and 64 °C with a variation of reaction time for 10, 20, 30, 60, 90, 120, and 150 min. Determination of activation energy and frequency factor was performed in a temperature of 29, 33, 38, 44, 49, 57 and 64 °C. The results showed that the optimum reaction time is 90 min. The activation energy (Ea) and frequency factor (A) was 44.5 kJ/mol and 4.780 x 103 sec-1, respectively.
FRACTIONATION OF FATTY ACID OMEGA 3, 6 AND 9 FROM SNAIL (Achatina fulica) USING COLOUM CHROMATOGRAPHY Haryadi, Winarto; Triono, Sugeng
Indonesian Journal of Chemistry Vol 6, No 3 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.21739

Abstract

The extraction of fat from snail has been carried out by Soxhlet extractor with petroleum ether solvent. Fatty product from extraction was transesterificated in BF3/methanol solvent for an hour by reflux procedure, then extracted by n-hexane to produce methyl ester fatty acid. Free water fatty acid methyl ester was analyzed by Gas Chromatography-Mass spectrometer (GC-MS). Fatty acid ester was separated from its fractions use column chromatography in n-hexane, n-hexane:dietil eter (2:1 v/v), dietil eter, aseton, ethanol and methanol. This fractions wer also analized by GC-MS. From GC-MS data sheet can be obtained 5 fractions which details are; fraction 1 contains omega 3: 27.54 %, omega 6: 15.40 % and omega 9: 6.77 %. Fraction 2 contains omega 3: 3.08 %, omega 6: 15.62 % and omega 9: 10.72 %. Fraction 3 contains omega 6: 3.57 %, omega 9: 7.02 % and none omega 3 inside it. Omega 3, 6 and 9 cant be identification in fraction 4 and 5.   Keywords: extraction, transesterification, column chromatography, GC-MS