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Peran PEG 400 dalam pembuatan lembaran bioplastik polohidroksialkanoat yang dihasilkan oleh Ralstonia eutropha dari Substrat Hidrolisat Pati Sagu Syamsu, Khaswar; Hartoto, Liesbetini; Fauzi, Anas Miftah; Suryani, Ani; Rais, Dede
Jurnal Ilmu Pertanian Indonesia Vol 12, No 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The purpose of the research was to investigate the effects of PEG 400 addition on the characteristics of bioplastic polyhydroxyalkanoates (PHA). PHA was obtained by cultivating Ralstonia eutropha on hydrolysed sago starch substrate using fed batch method for approximately 96 hours. The biomass concentration obtained was 4 g/L with PHA yield 20-30% of dry cell weight. The bioplastic was formed with solution casting method in which chloroform was used as solvent and PEG 400 was used as plasticizer. The concentrations of PEG 400added were 10, 20, and 30% (w/w), respectively. Bioplastic properties which were tested were tensile strength, elongation to break, density, thermal properties, cristalinity, and functional group. The addition of plastisizer tend to increase tensile strength and elongation to break, but decrease density, cristalinity and melting point. Bioplastic with 30% PEG 400 addition gave the best results. Bioplastic with 30% PEG 400 gave a value of tensile strength of 0.083 MPa; elongation to break of 0.881%; density of 0.7881 g/cm3;  melting point of 158.95 ac; and cristalinity of 44.58%. With these properties, the resulted bioi plastic may be used for surgical strings. Keywords: Bioplastic, Polyhydroxyalkanoates (PHA), Ralstonia eutropha, hidrolysed sago starch, PEG 400
PRODUKSI SIKLODEKSTRIN DARI PATI GARUT MENGGUNAKAN BERBAGAI KOMBINASI ENZIM [Production of Cyclodextrin from Arrowroot Starch by Using the Combination Enzymes] Noor, Erliza; Hartoto, Liesbetini
Jurnal Teknologi Dan Industri Pangan Vol 22, No 2 (2011): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

PRODUKSI SIKLODEKSTRIN DARI PATI GARUT MENGGUNAKAN BERBAGAI KOMBINASI ENZIM [Production of Cyclodextrin from Arrowroot Starch by Using the Combination Enzymes] Erliza Noor* dan Liesbetini Hartoto Departemen Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Institut Pertanian Bogor   Diterima 26 November 2009 / Disetujui 27 Oktober 2011 ABSTRACT   The research was aimed to produce cyclodextrin from arrowroot starch using a combination of starch hydrolysis enzymes (a-amylase, b-amylase, pullulanase and glucoamylase). Cyclization process to form cyclodextrin was obtained using Cyclodextrin-glycosyl-transferase (CGTase). a-amylase showed better performance and faster to hydrolyze arrowroot starch as compared to that of β-amylase. Glucoamylase also was gave better result than pullulanase to break the α-1,6-glycosidic chain. Combination of α-amylase and glucoamylase were more efficient for hydrolysis process and cyclodextrin production.   Key words: arrowroot starch, cyclodextrin, cyclodextrin-glycosyl-transferase, a-amylase, b-amylase, pullulanase, glucoamylase
Isolasi Dan Penapisan Aktinomisetes Laut Penghasil Antimikroba Sunaryanto, Rofiq; Marwoto, Bambang; Irawadi, Tun Tedja; Hartoto, Liesbetini
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 14, No 2 (2009): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

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Abstract

 Telah diiakukan isolasi dan penapisan aktinomisetes laut yang mampu menghasilkan senyawa antimikroba. Isolasi diiakukan ditiga tempat berbeda yaitu di Pantai Barat Banten, Pantai Utara Cirebon, dan Pantai Selatan Yogyakarta. Isolasi dilakukan dengan dua metode pre-treatment yaitu dengan metode pengasaman dan metode pemanasan. Dari hasil isolasi diperolehjumlah total isolat sebanyak 50 isolat. Setelah diiakukan penapisan diperoleh 4 isolat yang mampu menghambat Eschereschia coli, 5 isolat mampu menghambat Streptococcus aereus, 4 isolat mampu menghambat Bacillus subtilis, 4 isolat mampu menghambat Pseudomonas aeroginosa, 5 isolat mampu menghambat Candida albican, dan 4 isolat mampu menghambatAspergillus niger. Hasil identifikasi morfologi dan DNA dari salah satu isolat yang memiliki aktivitas antibakteri paling kuat (isolat A11) adalah Streptomyces sp. Secara morfologi isolat A11 memiliki hifa yang bercabang dengan kantong spora pada ujung hifa. Minimum Inhibition Concentration (MIC) isolat A11 terhadap Bacillus subtilis sebesar 120,86 μg/ml.Kata kunci: Isolasi, penapisan, aktinomisetes laut, antimikrobaIsolation and screening of antimicrobial-producing marine actinomycetes has been conducted on isolates taken from West Banten, North Cirebon, and South Yogyakarta Coasts. Two methods pretreatments were applied i.e. acid and heat shock method. The research 50 isolates. The screening reavealed four isolates which has ability to inhibi Eschereschia coli, 5 isolates could inhibited Streptococcus aereus, 4 isolates could inhibited Bacillus subtilis, 4 isolates could inhibited Pseudomonas aeroginosa, 5 isolats could inhibited Candida albican, and 4 isolatescould inhibited Aspergillus niger. Result of  identification morphology and DNA of isolate A11 it’s Streptomyces sp. Morphology of isolate A11 haves branching hyphae with spore sack at the end of hyphae. The Minimum Inhibition Concentration (MIC) of isolate A11 to Bacillus subtilis was 120,86 μg /ml.Key words: Isolation, Screening, Marine Actinomycetes, Antimicrobial.
Isolation and Characterization of Antimicrobial Substance from Marine Streptomyces sp. SUNARYANTO, ROFIQ; MARWOTO, BAMBANG; IRAWADI, TUN TEDJA; HARTOTO, LIESBETINI
Microbiology Indonesia Vol 4, No 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

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Abstract

Isolation and purification of antimicrobial active substance produced by marine Actinomycetes has been carried out.  Marine sediment samples were obtained from six different places at Banten West Coast.  Isolation was conducted using two pretreatment methods,  acid and heat shock pre-treatment.  A total of 29 Actinomycetes isolates were obtained from the various sediment samples collected, then tested for antimicrobial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Pseudomonas aeruginosa ATCC27853, Bacillus subtilis ATCC 66923, Candida albicans BIOMCC00122, and Aspergillus niger BIOMCC00134.  Among the isolates, isolate A11 was the most activity to Gram-positive and Gram-negative bacteria, and morphological observation and identification using 16S rRNA showed that the isolate was similar to Streptomyces sp.  Production of active compound from A11 isolate used yeast peptone medium.  Purification of active compounds was carried out using silica-gel-column chromatography and preparative HPLC.  A single peak of active compounds was detected by HPLC, which showed a retention time of 8.35 min and maximum absorbance in UV visible at 210 nm and 274.5 nm respectively.
Cyclo (Tyrosyl-Prolyl) Produced by Streptomyces sp.: Bioactivity and Molecular Structure Elucidation SUNARYANTO, ROFIQ; MARWOTO, BAMBANG; HARTOTO, LIESBETINI; IRAWADI, TUN TEDJA
Microbiology Indonesia Vol 5, No 2 (2011): June 2011
Publisher : Indonesian Society for microbiology

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Abstract

Determination of bioactivity by minimum inhibitory concentration (MIC) methods and molecular structure identification of antibiotic produced by Streptomyces sp. have been carried out. The antibiotic was produced by liquid culture using Streptomyces sp. isolate. Purification of antibiotic was carried out by silica gel column chromatography and preparative HPLC. Molecular structure identification was carried out using ESI-MS, 1H NMR, 13C NMR, and 13C DEPT NMR. Pure antibiotic showed inhibition activity to Gram-negative and Gram-positive bacteria. MIC to Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC27853 , Staphylococcus aureus ATCC25923, and Bacillus subtilis ATCC66923 were 27.0, 68.7, 80.2, and 73.7 μg mL-1 , respectively. Identification using ESI-MS showed that the molecular weight of this antibiotic was 260 g mol-1 , and molecular formula was C14H16N2O3 . Elucidation of molecular structure using 1HNMR, 13C NMR, and 13C DEPT NMRshowed that antibiotic was cyclo(tyrosyl-prolyl).
Peran PEG 400 dalam pembuatan lembaran bioplastik polohidroksialkanoat yang dihasilkan oleh Ralstonia eutropha dari Substrat Hidrolisat Pati Sagu Syamsu, Khaswar; Hartoto, Liesbetini; Fauzi, Anas Miftah; Suryani, Ani; Rais, Dede
Jurnal Ilmu Pertanian Indonesia Vol 12, No 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Original Source | Check in Google Scholar | Full PDF (164.904 KB)

Abstract

The purpose of the research was to investigate the effects of PEG 400 addition on the characteristics of bioplastic polyhydroxyalkanoates (PHA). PHA was obtained by cultivating Ralstonia eutropha on hydrolysed sago starch substrate using fed batch method for approximately 96 hours. The biomass concentration obtained was 4 g/L with PHA yield 20-30% of dry cell weight. The bioplastic was formed with solution casting method in which chloroform was used as solvent and PEG 400 was used as plasticizer. The concentrations of PEG 400added were 10, 20, and 30% (w/w), respectively. Bioplastic properties which were tested were tensile strength, elongation to break, density, thermal properties, cristalinity, and functional group. The addition of plastisizer tend to increase tensile strength and elongation to break, but decrease density, cristalinity and melting point. Bioplastic with 30% PEG 400 addition gave the best results. Bioplastic with 30% PEG 400 gave a value of tensile strength of 0.083 MPa; elongation to break of 0.881%; density of 0.7881 g/cm3;  melting point of 158.95 ac; and cristalinity of 44.58%. With these properties, the resulted bioi plastic may be used for surgical strings. Keywords: Bioplastic, Polyhydroxyalkanoates (PHA), Ralstonia eutropha, hidrolysed sago starch, PEG 400
PRODUKSI SIKLODEKSTRIN DARI PATI GARUT MENGGUNAKAN BERBAGAI KOMBINASI ENZIM [Production of Cyclodextrin from Arrowroot Starch by Using the Combination Enzymes] Noor, Erliza; Hartoto, Liesbetini
Jurnal Teknologi dan Industri Pangan Vol 22, No 2 (2011): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Original Source | Check in Google Scholar | Full PDF (371.738 KB)

Abstract

The research was aimed to produce cyclodextrin from arrowroot starch using a combination of starch hydrolysis enzymes (a-amylase, b-amylase, pullulanase and glucoamylase). Cyclization process to form cyclodextrin was obtained using Cyclodextrin-glycosyl-transferase (CGTase). a-amylase showed better performance and faster to hydrolyze arrowroot starch as compared to that of β-amylase. Glucoamylase also was gave better result than pullulanase to break the α-1,6-glycosidic chain. Combination of α-amylase and glucoamylase were more efficient for hydrolysis process and cyclodextrin production.
Designing Packed Palm Cooking Oil Distribution at Traditional Market in Jakarta Using Fuzzy Clustering Utami, Teja Primawati; Ma’arif, Syamsul; Arkeman, Yandra; Hartoto, Liesbetini
Jurnal Penelitian Pascapanen Pertanian Vol 12, No 3 (2015): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

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Abstract

Referring to the Indonesian National Standard number 7709 year 2012 that palm cooking oil should be fortified with vitamin A, so that the distribution process required packaging to protect Vitamin A. Besides packaging palm cooking oil can make the content hygiene. Packaged cooking oil was distributed from the factory to the traditional market directly. In accordance with Regulation of the Minister of Industry of the Republic of Indonesia Number 87 year 2013 on the application of ISO palm olein is mandatory and Trade Minister Regulation number 80 year 2013 on compulsory packaging cooking oil, so need a mechanism to bulk cooking oil distribution.Simple packaging in the traditional market of producers to effective and efficient consumer. The purpose of this paper is to design a system of distribution of cooking oil from producers to consumers in traditional markets by creating a central cluster calls distribution center. Design models created using fuzzy clustering method. The results of this study create 15 clusters of traditional markets in Jakarta.Merujuk pada Standar Nasional Indonesia nomor 7709 tahun 2012 bahwa minyak goreng sawit perlu ditambahkan vitamin A, sehingga dalam proses distribusinya diperlukan pengemasan untuk melindungi Vitamin A tersebut. Tujuan pengemasan minyak goreng curah ke kemasan sederhana juga dilakukan untuk menjaga higienitas dan melindungi konsumen dari praktek pengoplosan. Sesuai dengan Peraturan Menteri Perindustrian Republik Indonesia nomor 87 tahun 2013 tentang pemberlakuan SNI minyak goreng sawit secara wajib dan Peraturan Menteri Perdagangan nomor 80 tahun 2013 tentang minyak goreng wajib kemasan, maka diperlukan suatu mekanis pendisribusian minyak goreng curah ke kemasan sederhana di pasar tradisional dari produsen ke konsumen yang efektif dan efisien. Tujuan dari makalah ini adalah mendesain sistem distribusi minyak goreng dari produsen ke konsumen di pasar tradisional dengan cara membuat klaster yang otomatis ditentukan sentral distribusinya. Desain model dibuat dengan menggunakan metode Fuzzy clustering. Hasil dari penelitian ini adalah terdapat 15 klaster pasar tradisional di Jakarta dengan masing-masing satu sentra distribusi.
ANTIBIOTIC COMPOUND FROM MARINE ACTINOMYCETES (Streptomyces sp A11): ISOLATION AND STRUCTURE ELUCIDATON Sunaryanto, Rofiq; Marwoto, Bambang; Irawadi, Tun Tedja; Mas’ud, Zainal Alim; Hartoto, Liesbetini
Indonesian Journal of Chemistry Vol 10, No 2 (2010)
Publisher : Universitas Gadjah Mada

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Abstract

Purification and structure elucidation of antibiotic produced by marine actinomycetes (Streptomyces sp A11) was conducted. Production of antibiotic was carried out by liquid fermentation using yeast and peptone medium for 5 days fermentation. Purification of antibiotic was carried out by silica gel 60 (Merck, 0.063-0.200 mm) column chromatography and preparative HPLC. Structure elucidation was carried out using ESI-MS, 1H NMR, 13C NMR, DEPT 13C NMR, and FTIR. This antibiotic was identified as cyclo (tyrosyl-prolyl) / (C14H16N2O3). This antibiotic had biological activity to Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Bacillus subtilis ATCC 66923, Pseudomonas aeruginosa ATCC27853, and produced by extracellular secretion.   Keywords: antibiotic, actinomycetes, purification, structure elucidation
Isolasi dan Elusidasi Struktur Kimia Antimikroba yang Dihasilkan Oleh Aktinomisetes Laut Sunaryanto, Rofiq; Irawadi, Tun Tedja; Hartoto, Liesbetini
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 5, No 1 (2010): Juni 2010
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

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Abstract

Isolasi senyawa aktif dari aktinomisetes laut yang memiliki aktivitas antimikroba telah dilakukan. Isolasi aktinomisetes dilakukan dengan pengenceran dan praperlakuan sampel dengan cara pemanasan dan pengasaman. Sampel diambil dari sedimen laut di 6 titik lokasi Pantai Anyer, Banten. Dari total 29 isolat yang diperoleh, isolat A32 merupakan isolat terpilih untuk penelitian lebih lanjut. Isolat A32 memiliki aktivitas antimikroba terhadap Staphylococcus aureus ATCC25923 dan Candida albican BIOMCC00122. Elusidasi struktur kimia menggunakan ESI-LCMS, 1 HNMR, dan COSY menunjukkan bahwa senyawa aktif tersebut memiliki bobot molekul 501,2 g/mol dan rumus molekul C26H35N3O7. Diduga senyawa ini termasuk dalam golongan makrolakton virginiamycin yaitu Madumycin I.