Articles

Association of Sexual Maturation and Body Size of Arfak Children KAWULUR, ELDA IRMA JEANNE JOICE; SURYOBROTO, BAMBANG; BUDIARTI, SRI; HARTANA, ALEX
HAYATI Journal of Biosciences Vol 19, No 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.19.3.124

Abstract

Gonad maturation in pubertal girls and boys is accompanied with somatic growth spurt, changes in quantity and distribution of body fat (BF), development of secondary sex characters, and relevant physiological events. Menarche (first event of menstruation) and spermarche (first event of nocturnal sperm emission) are usually used as indicators of gonad maturation. We found that median age at menarche of Arfak girls in Manokwari, West Papua is 12.2 years, while median age at spermarche of boys is 13.6 years. A possible factor causing young age at menarche is due to adaptation to unstable environmental conditions because of high risk of mortality by malaria disease during childhood. The events of menarche and spermarche achieved one year after the peak body height (BH) velocity, and just before or at the same time with the time of maximum growth rate of body weight (BW), body mass index (BMI), and BF. The average BMI of Arfak girls was big at 21.9 kg/m2 at the time of their menarche. Bigger average BMI might be caused by prepubertal slowing down of BH growth compare to growth of BW whichis still increasing. Girls accumulate BF before puberty to be used as an energy reserve for the occurrence of menarche. At the time of development of secondary sexual characters girls use the fat reserve so it decline sharply after puberty. In boys, growth rate of BF was stopped at 11 years old, and then growing negatively presumably because boys use fat mass for the occurence of spemarche. BF growth rate reached the lowest point at the age 16 years old, and then increase linearly with age through adolescence until adulthood at age 23 years old.
The Growth of Body Size and Somatotype of Javanese Children Age 4 to 20 Years WIDIYANI, TETRI; SURYOBROTO, BAMBANG; BUDIARTI, SRI; HARTANA, ALEX
HAYATI Journal of Biosciences Vol 18, No 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.4.182

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Growth of body size (body height and body weight) and somatotype in 363 girls and 299 boys aged 4 to 20 years of ethnic Javanese lived in Magelang Regency Indonesia were studied cross-sectionally. Over half of them were categorized in the well-off family, therefore underweight or underfat prevalence in our subjects was low (14.3%) but overweight and obesity prevalence was also low (14%). They were shorter and lighter than reference children from U.S., Japan and Yogyakarta but they improved when compared with those of the same ethnic of Bantul and with the different ethnic of rural India. There was a clear age-related change of their somatotype. At age 4 years, the physique of children subjects in both sexes is found to be mesomorph-endomorph. Thereafter it is transformed into ectomorphic-endomorph in girls and to mesomorph-ectomorph in boys at the age of 20 years. In girl subjects, the onset of puberty was characterized by an acceleration of endomorphy component at age 8 years. While in our boys it was characterized by an acceleration of ectomorphy since age 9 years. The different growth pattern of somatotype components showed that the use of BMI as an indicator of fatness in children should be reassessed.
Phylogenetic Study of Mangifera laurina and its Related Species Using cpDNA trnL-F Spacer Markers Fitmawati, .; Hartana, Alex
HAYATI Journal of Biosciences Vol 17, No 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.17.1.9

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Phylogenetic study of cpDNA intergenic spacer trnL-F of Mangifera laurina and their related species within the genus Mangifera in Indonesia was conducted using Rutaceae as the outgroup. This study was to reconstruct phylogenetic relationships and to understand infraspecific relationships within Mangifera based on cpDNA trnL-F intergenic spacer sequences. The results showed that Mangifera sp. Hiku (mangga hiku) as the basic cultivar in the clade, and it supported the monophyletic group in Mangifera. And phylogenetic construction indicated that Mangifera sp. Hiku was the progenitor of M. laurina and their related species.
Hubunagn kemampuan pergantian inang dengan plastisitas genetika pada cendawan blas padi (Pyricularia grisea) Listiyowati, Sri; Widyastuti, Utut; Rahayu, Gayuh; Hartana, Alex; Jusuf, Muhammad
Jurnal Ilmu Pertanian Indonesia Vol 14, No 2 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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The Digitaria ciliaris, wild grass grown around rice field, was a host for Pyricularia grisea (Cooke) Sacc., the fungi caused blast disease of rice. This fungi have a specific mechanism to regenerate new genetic variation in its life cycle. The aim of this research is to study the relation between the ability of the fungi to infect different species of host with its genetic plasticity. It was used three SCAR molecular markers Cutl, Pwl 1 and Erg2. P.grisea isolates (Dc4J1) originated from D. ciliaris at Jasinga-Bogor were able to infect rice cultivars Kencana Baliand Cisokan. The original Dc4Jl, from D. ciliaris, and the Dc4Jl that were reisolated from the infected rice cultivars (reisolates-1) had the same ability to infect Kencana Bali and Cisokan. Molecular technique showed that there was a different molecular marker genotype between the original Dc4J1, from D. ciliaris, and the Dc4Jl reisolated from infected rice cultivars. The original Dc4J1 owned Cutl but did not Pwl2 in contrary the reisolatesDc4J1 from rice cultivars (reisolates-1) had Pwl2 but did not Cutl. The Erg2 presented in both the original and the reisolated Dc4Jl. These results indicated that there were a change of genotype of P. grisea at the same time with the change of host species. The Dc4Jl isolates originated from Kencana Bali and Cisokan (reisolates-2) that were infected by reisolate-1, had the same genotype with the reisolates-1. Keywords: Digitaria ciliaris, Pyricularia grisea, genetic plasticity.
PERTUMBUHAN TANAMAN JARAK PAGAR (Jatropha curcas L.) PADA LAHAN PASCA TAMBANG TIMAH DI BANGKA YANG DIBERI PUPUK ORGANIK Gedoan, Sukmarayu P; Hartana, Alex; Hamim, Hamim; Widyastuti, Utut; Sukarno, Nampiah
JURNAL ILMIAH SAINS Vol 11, No 2 (2011): Volume 11 Nomor 2, Oktober 2011
Publisher : Sam Ratulangi University

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PERTUMBUHAN TANAMAN JARAK  PAGAR (Jatropha curcas L.) PADA LAHAN PASCA TAMBANG TIMAH DI BANGKA YANG DIBERI PUPUK ORGANIK Sukmarayu P. Gedoan1), Alex Hartana2), Hamim2), Utut Widyastuti2) dan Nampiah Sukarno2) 1)Program Studi Biologi, FMIPA Universitas Negeri Manado di Tondano; 2)Departemen Biologi, FMIPA, Institut Pertanian Bogor, Bogor 16680   ABSTRAK Kegiatan penambangan timah menyebabkan perubahan karakteristik fisika dan kimia tanah sehingga menjadi tidak sesuai untuk pertumbuhan tanaman.  Tujuan penelitian ini untuk mempelajari pertumbuhan 7 aksesi jarak pagar yang ditanam pada lahan pasca tambang timah yang diberi kompos dan kotoran sapi.  Penelitian ini dilakukan di TSS 133, Kelurahan Sinar Baru, Kabupaten Bangka, Provinsi Bangka Belitung.  Penelitian lapangan dilaksanakan pada Bulan Mei 2007 sampai dengan Bulan Desember  2008.  Percobaan faktorial ini dirancang dalam Rancangan Petak Terbagi dengan tiga ulangan.  Petak utama adalah 7 aksesi jarak pagar yang terdiri atas: aksesi Madiun, Ponorogo, Jember, Dompu, Lampung, Bengkulu,  dan Sukabumi, sedangkan  anak petak berupa tanah tanpa pemberian kompos dan pupuk kandang (kontrol), kompos trubus 4 kg/lubang ditambah 4 kg tanah bagian atas  dan  kotoran sapi 4 kg/lubang ditambah 4 kg tanah bagian atas. Hasil penelitian menunjukkan bahwa pertumbuhan awal yang paling baik untuk tanah bekas tambang timah adalah pada aksesi Jember yang ditunjukkan oleh diameter batang, berat kering tanaman, berat kering tajuk dan berat kering akar.  Sedangkan tinggi tanaman tertinggi diamati pada aksesi Madiun.  Penambahan kotoran sapi dapat meningkatkan produksi biji dan kandungan minyak.  Produksi biji tertinggi diperoleh pada aksesi Bengkulu dan kandungan minyak tertinggi diperoleh pada aksesi Dompu. Kata kunci: aksesi, Jatropha curcas, kompos, kotoran sapi   THE GROWTH OF CASTOR OIL PLANT (Jatropha curcas L.) ON THE POST-TIN-MINING LAND IN BANGKA PROVIDED WITH ORGANIC FERTILIZER ABSTRACT The activity of tin mining changes the physical and chemical characteritics of soil, so that the soil isnot suitable for plant growth.  The objective of this research was to evaluate the growth of some accession of Jatropha curcas that we planted on post-tin-mining land provided with compost and cow feces.   This research was conducted in TS 133, Sinar Baru Village, District of Bangka, Province of Bangka Belitung.  This field research was carried out in May 2007 to December 2008.  Factorial experiment was designed as split plot with three replications.  The main plot was 7 accessions, i.e. Madiun, Ponorogo, Jember, Dompu, Lampung, Bengkulu, and  Sukabumi.  The subplot waskonds of organic fertilizer, i.e. top soil without addition of compost and cow fecer (control), trubus compost  (4 kg/hole) added with top soil (4 kg/hole), and cow feces (4 kg/hole) added with topbsoil (4 kg/hole). The result showed that Jember accession had the best early growth based on the observation of stem diameter, plant dry weight, and root dry weight.  The largest plant height was observed in Madiun accession.  The higest seed production was observed in Bengkulu accession and Dompu accession had the higest oil content.Keywords: accession, Jatropha curcas, , compost, cow feces
Pollen Sensitivity among Respiratory Allergic Patients Rengganis, Iris; Hartana, Alex; Guhardja, Edi; Djauzi, Samsuridjal; Budiarti, Sri
Journal of the Indonesian Medical Association Vol. 58 No. 9 September 2008
Publisher : Journal of the Indonesian Medical Association

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Abstract

Allergy is a type I hypersensitivity reaction, which occurs when the body produces an excess of IgE antibody as a response to allergens. The development and severity of allergic diseases depend on a complex interaction between genetic and environmental factors. Pollen are one of the main environmental allergens. The allergenic pollens of the atmosphere varies according to climate, geography and vegetation. This research is conducted to study the pollen sensitivity reaction commonly occured among respiratory allergic (bronchial asthma and allergic rhinitis) patients in Jakarta. Total protein pollens of palm trees, coconut, pine, acacia, grass, maize, and rice were analyzed in denatured-state by SDS-PAGE and are dominated by proteins with molecular weight of 10-70 kD. Skin prick test with allergen protein of pollen were tested to sixty nine subjects whom have been diagnosed with respiratory allergic and sixty nine subjects without history of allergy. The results among respiratory allergic patients, showed that pollen from grass and acacia demonstrated a higher percentage of sensitivity response compared with other pollen.Keywords: pollen, protein, sensitivity, skin prick test, allergen
The Genetic Estimation of Fruit Component Parameters of Seven Coconut Populations Without a Progeny Test Asmono, Dwi; Hartana, Alex; Guhardja, Edi; Yahya, Sudirman
Forum Pasca Sarjana Vol 16, No 1 (1993): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Heritability of fruit characters, such as fruit weight, husk weight, husk thickness, sheal weight, endosperm (water) weight, albumen weight, copra weight, oil content, fruit length and ftuit width. have been estimated on seven coconut populations. The results showed that most of the fruit components of Malayan Red Dwarf (MRD) and Malayan Yellow Dwarf (Mm)had relatively high heritability (>0.80), except fruit weight of MRD (0.74) and albumen weight of MYD (0.78). On the other hand, the heritability of those characters in tall populatidns varied between population. All of fruit characters on Polynesian Tall (PYT) and Seruwai Tall (SAT) had relatively high heritability (>0.80). On West African Tall (WAT), most Of the heritability of the fruit characters were relatively high (>0.80). except husk weight (0.75). endosperm weight (0.79) and oil content (0.41). Heritability of seven characters of Bali Tall (BLT) were relatively high, but those of three characters were relatively low; i.e. husk weight (0.52). fruit width (0.56) and husk thickness (0.71). Three fruit characters of Rmnell Tall (RLT) shown higher heritability. those are husk thickness (0.87), endosperm weight (0.92) and fruit length (0.90).
Taksonomi Mangga Budidaya Indonesia dalam Praktik Fitmawati, ,; Hartana, Alex; Purwoko, Bambang S.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 37, No 2 (2009): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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The classification  of  cultivated plants should meet two approaches namely the botanical (classifying based on essential systematic plant characters) and the practical approach (clustering based on the analysis of commercial properties). This study analyzed taxonomy of mango cultivars grown in Indonesia based on morphology and agronomy characters. It obtained 84 recognizable cultivars.They are grouped into eigth main cultivar-groups (e.g. Berem, Madu, Gedong, Golek, Bapang, Arumanis, Kepodang, and Kebo) and eigthteen cultivar-groups. The ´Lalijiwo´ cultivars synonym with ´Thaber´, ´Tabar´, ´Gurih´ whereas ´Arummanis´ synonim with ´Gadung´. Meanwhile, the cultivar ´Kates277´ which is a member of Golek cultivar main group is homonim with the cultivar ´Kates´ in Arummanis cultivars main group. Furthermore, the cultivar Nanas93 (one of Madu main group cultivar) that different with  the cultivar ´Nanas71´ which is categorized as member of Bapang main cultivar group.   Key words: Taxonomy of cultivated plant, Indonesian mango, morphology, agronomy characters
Diversity of SCAR Markers of Pyricularia grisea Isolated from Digitaria ciliaris Following Cross Infection to Rice LISTIYOWATI, SRI; WIDYASTUTI, UTUT; RAHAYU, GAYUH; HARTANA, ALEX; JUSUF, MUHAMMAD
Microbiology Indonesia Vol 5, No 1 (2011): March 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.5.1.1

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Cross infection of Pyricularia grisea from grass to rice and vice versa has been reported, but genetic changes are not known yet.  This research  aimed at estimating the possibility of the genotype alteration in P. grisea dc4 isolated from Digitaria ciliaris, following cross infection to either rice cv. Kencana bali, Cisokan, and IR64 or Panicum repens, Cynodon dactylon, Digitaria sp., and Ottochloa nodosa. The genotypes were analyzed by employing three SCAR markers, Cut1; PWL2; and Erg2.  The results indicated that the dc4 was only able to infect Kencana bali, Cisokan, and P. repens.  The dc4 had only two out of three SCAR markers, Cut1 and Erg2.  Host shift was followed by genotype alteration in two loci of SCAR.  Isolates derived from lesions on Kencana bali (dc4-kb) and Cisokan (dc4-c) of the dc4 infection, both lost their Cut1 and gained PWL2. On the contrary, there was no genotype alteration from dc4 to isolate derived from P. repens of dc4 infection (dc4-pr).  Neither the isolate dc4-kb  that was cross-inoculated to Cisokan nor the dc4-c that was cross-inoculated to Kencana bali showed SCAR marker change.  In comparison,  race 173 isolate and those derived from Kencana bali and Cisokan did not show genotype alteration. All had two out of three SCAR markers, PWL2 and Erg2.  The isolate 173 was adapted to rice. This indicated that genotype diversity of the dc4 might arise following host shift from grass to rice.Pyricularia grisea merupakan cendawan blas yang telah diketahui memiliki kisaran inang luas selain pada padi. Infeksi silang cendawan blas pada rumput ke padi dan sebaliknya telah dilaporkan, tetapi perubahan genetiknya belum dilaporkan. Tujuan penelitian ini menganalisis kemampuan infeksi silang dan perubahan genotipe P. grisea dc4 asal Digitaria ciliaris dalam perpindahannya ke padi cv. Kencana bali, Cisokan, dan IR64 atau rumput Panicum repens, Cynodon dactylon, Digitaria sp. dan Ottochloa nodosa. Genotipe P. grisea dianalisis melalui tiga marka SCAR, yaitu Cut1; PWL2; dan Erg2. Isolat dc4 memiliki 2 marka SCAR, yaitu Cut1 dan Erg2; tidak memiliki PWL2. Isolat dc4 hanya mampu menginfeksi silang Kencana bali, Cisokan, dan P. repens. Turunan isolat dc4 sebagai hasil infeksi silang ke Kencana bali (dc4-kb) dan Cisokan (dc4-c) menunjukkan perubahan genotipenya, yaitu Cut1 tidak teramplifikasi pada keduanya; PWL2 teramplifikasi;, serta Erg2 tetap teramplifikasi. Sebaliknya, turunan isolat dc4 sebagai hasil infeksi silang ke P. repens (dc4-pr) tidak mengalami perubahan genotipe. Turunan isolat dc4-kb sebagai hasil infeksi silang ke Cisokan, maupun turunan isolat dc4-c dari Kencana bali, juga tidak menunjukkan perubahan genotipe, yaitu tetap menunjukkan keberadaan PWL2 dan Erg2. Sebagai pembanding digunakan isolat ras 173 yang diisolasi dari padi.  Genotipe isolat tersebut maupun turunannya, sebagai hasil infeksi silang ke Kencana bali dan Cisokan, tidak menunjukkan perubahan. Perubahan genotipe dc4 terjadi mengikuti pergantian inang dari rumput ke padi.
Komunitas Mikrofungi pada Lapisan Horizon Serasah Acacia mangium Samingan, Samingan; Sudirman, Lisdar I.; Setiadi, Dede; Hartana, Alex; Tjahjono, Budi
Jurnal Agrista Vol 12, No 2 (2008): Volume 12 Nomor 2 Agustus 2008
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Microfungal Community on Litter Horizon Layer of Acacia mangiumABSTRACT. Fungal diversity on litter horizon layer of Acacia mangium were investigated to examined fungal species and fungal community on each litter horizon layer, and also to examined relationship between organic content of litter and fungal community. Twenty two species were isolated from three litter horizon layer with dilution method. Total fungal population on five years old A. mangium standing was higher than two years old, whereas on logging former area was low. Total fungal population on standing two and five years old were highest on L layer follow by F and H layer, but on logging former area were highest F layer followed by L and H layer. Aspergillus was dominate on H layer in almost of sampling collection area, beside that Aspergillus and Penicillium were found also on L and F layer. Generally L and F layer dominated by Sp7, Sp5, Sp20, and Sp22. The highest diversity indice on two years old standing was found at different layer.; L and H on health and Genoderma attacked standing, whereas on standing five years old, highest diversity indices was found at L layer, but on logging former area highest diversity indices was found at H layer.