Tjodi Harlim
Faculty of Mathematic and Natural Science, Chemistry Department, Hasanuddin University, Makasar.

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OPTIMALISASI EKSTRAKSI STEROID UTAMA DARI BEBERAPA JENIS IKAN DENGAN SISTEM PELARUT TIGA KOMPONEN Mangallo, Bertha; Harlim, Tjodi; Taba, Paulina
MARINA CHIMICA ACTA Vol 5, No 1
Publisher : Universitas Hasanuddin

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Abstract

The aim of this research is to determine an optimal time and an appropriate method of extracting the main  steroid  content as  well  as to determine the sterol level in some types of sea and fresh water fishes (Lutjhanus malabaricus,  Thunnus  albacore,  Tilapia mossambica  and Chanos chanos Forskau). A method used was an extraction method with a solvent system of three components (chloroform, methanol and water) in a ratio of 1: 2: 0.8. Samples were extracted with an extraction time of 5, 10, 15 and 20 minutes and with two extraction methods (cold extraction, room temperature and hot extraction at 40 oC). The extraction result was a biphase solution; the upper layer was a methanol-water extract and the bottom layer was a chloroform extract, which was a lipid fraction. The existence of sterol in the lipid extract was examined with the Liebermann-Burchard reagent. The chloroform extract was then saponificated with potassium hydroxide (10 %) in methanol and the sterol level in the isolate was determine with a UV-Vis spectrophotometer. Results showed that the maximum sterol level was obtained at the extraction time of 10 minutes and with the extraction method at a temperature of    40 oC. From the four kind of fishes investigated, it was clear that  the maximum  content of  sterol was  found in Lutjhanus malabaricus (0,31 %), whereas the  minimum  content was in Chanos chanos Forskau (0,18 %).   Keywords: extraction, sterol, and fish
DAYA HAMBAT EKSTRAK ALGA COKLAT JENIS SARGASSUM TERHADAP PROSES PEMATANGAN BUAH DAN SAYUR Marzuki, Ismail; Harlim, Tjodi; Ubbe, Umar
MARINA CHIMICA ACTA Vol 4, No 2 (2003): Oktober - 2003
Publisher : Universitas Hasanuddin

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Abstract

The aims of this research were to know the percentage of the brown algae (Sargassum) extract obtained from a cooking method by using of water as a solvent and to determine nitrogen and protein contents in the extract. Results shows that the extract of Sargassum obtained is 9,435 % with total nitrogen, protein, protein–nitrogen, and cytokinin-nitrogen are 3,86 %; 2,89 %; and 3,385 %, respectively. Two method were use to know the activity of Sargassum on the again process of fruits and vegetables (sweet orange, Hanlim norris ant tomato). The methods were as follows: (1) fruits and vegetables were directly wrapped with dried Sargassum and (2) fruits and vegetables were immersed into the sargassum extract with various concentration (between 0 and 50 ppm) with the concentration interval observed in the first method. This may happen because there was no a direct contact between fruits and vegetables with the component  which has the inhibition effect to the aging  process of fruits and vegetables. On the other hand, in the second method, the Sargassum extract is effective in inhibiting  the aging  process of fruits and vegetables. At a suspension concentration of  35  ppm, the sweet orange can be kept for about 70,4 days at a temperatur of 10 0C, whereas without a treatment with the suspension, it can just be kept for about 31,6 days. The effect to the suspension  with a concentration of 25 ppm to tomatoes are that the storing  time of the fruits can be until 45,4 days at a temperature of 10 0C. Without a treatment with the suspension, these tomatoes can just be kept for 19,4 days. This is possible due to the assumption that the component existing in the Sargassum extract can cover the pores of sweet oranges and tomatoes so that the air respiration from  and to the fruits and vegetables decrease. Keywords: Inhibition, extract, cytokinin, sargassum, aging
PENGARUH PENAMBAHAN TEMBAGA SULFAT TERHADAP PRODUKSI ENZIM POLIFENOL OKSIDASE DARI FUNGI ENDOFIT KULIT BUAH KAKAO Sartini, -; Patong, Abd Rauf; Harlim, Tjodi; Pirman, -
MAJALAH FARMASI DAN FARMAKOLOGI Vol 15, No 1 (2011)
Publisher : Universitas Hasanuddin

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Abstract

In medicine, polyphenol oxidases are used for prevention of bacterial adhesion, treatment of Parkinson’s disease and control of melanin synthesis. Polyphenol oxidase could be produce by endophytic fungus from podhusk of Theobroma cacao. To produce microbial enzyme, media composition should be optimized. The aim of this study was to investigate the effect of cupri sulphate concentration as inducer  in  fermentation medium containing 1 % yeast extract, 0,05 % MgSO4, 0.1% KH2PO4, 0,051 % gallic acid, and CuSO4.5H2O 0-50 ppm. The result showed that it was effect addition of CuSO4 in fermentation medium toward production of polyphenol oxidase. Optimal concentration of CuSO4 to produce polyphenol oxidase was 25 bpj with enzyme activity  530 U/mL using catechol as substrate.
Aktivitas Antibakteri Senyawa Turunan Oleanan dari Kayu Akar Pterospermum Subpeltatum Salempa, Pince; Noor, Alfian; Hariani, Nunuk; Harlim, Tjodi
Jurnal Natur Indonesia Vol 13, No 01 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

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Abstract

A triterpenoid derivative compound, oleane-12-en-2,3,23-triol-28-oat, from chloroform fraction of root wood ofPterospermum subpeltatum C. B. Rob (Sterculiaceae) was identified using IR, 1H dan 13C NMR data. The compoundhas shown toxicity against Artemia salina, LC50 of 46.06 μg/mL, and antibacterial potential upon Shigella boydii andStaphylococcus aureus with inhibition zones of 8.3 and 9.8 mm respectively.
SENYAWA KALKON BARU BERSIFAT ANTI-BAKTERI DARI TUMBUHAN CRYPTOCARYA COSTATA (LAURACEAE) Jalaluddin, M. Noor; Harlim, Tjodi; Hakim, Euis H.; Achmad, Sjamsul A. A.; Syah, Yana M.; Latip, Jalifah; Said, Ikram M.
Berkala Ilmiah MIPA Vol 16, No 1 (2006)
Publisher : FMIPA UGM

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Abstract

Suatu kalkon telah diisolasi dari kulit batang Cryptocarya costata. Isolat diperoleh dari fraksi kloroform, setelah fraksinasi dengan teknik kromatografi yang dilanjutkan dengan rekristalisasi dalam heksana dan etilasetat, dihasilkan kristal jarum berwarna kuning dengan titik leleh 167-169oC. Elusidasi struktur isolat berdasarkan spektrum UV, IR, 1 D dan 2D NMR, maka dapat ditetapkan bahwa senyawa isolat adalah 2’,4’-Dihidroksi-3’,6’-dimetoksicalkon. Uji sitotoksik terhadap E.coli, menunjukkan aktivitas positif dengan nilai hambat 35,4 %, dan pertama kali ditemukan dari tumbuhan Cryptocarya Kata kunci : Kalkon, Sitotoksik, Cryptocarya costata
THE POTENTIAL OF Clathria reinwardtii as BIOACCUMULATOR OF HEAVY METAL Cu Melawaty, Lydia; Noor, Alfian; Harlim, Tjodi; Voogd, Nicole de
Marina Chimica Acta Vol 15, No 2 (2014): Metals in Marine Samples and Age Determination using Liquid Scintillation Coun
Publisher : Hasanuddin University

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Abstract

Heavy metal Cu is an essential metal required by living organisms but a pollutant in water environment when existed in a level above the treshold level. The capability of sponge Clathria reinwardtii in accumulating heavy metals was analyzed according to modified Müller et al. (1998) method, and the sediment was analyzed according to Loring and Rantala in Rochyatun et al., (2006). Cu concentrations were measured by ICP-OES. The results indicate that Clathria reinwardtii was capable of accumulating the highest Cu level compared to other sponges. The highest Cu level analyzed in Clathria reinwardtii was 161.3 mg/kg.
THE POTENTIAL OF Clathria reinwardtii as BIOACCUMULATOR OF HEAVY METAL Cu Melawaty, Lydia; Noor, Alfian; Harlim, Tjodi; Voogd, Nicole de
Marina Chimica Acta Vol 15, No 2 (2014): Metals in Marine Samples and Age Determination using Liquid Scintillation Coun
Publisher : Hasanuddin University

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Abstract

Heavy metal Cu is an essential metal required by living organisms but a pollutant in water environment when existed in a level above the treshold level. The capability of sponge Clathria reinwardtii in accumulating heavy metals was analyzed according to modified Müller et al. (1998) method, and the sediment was analyzed according to Loring and Rantala in Rochyatun et al., (2006). Cu concentrations were measured by ICP-OES. The results indicate that Clathria reinwardtii was capable of accumulating the highest Cu level compared to other sponges. The highest Cu level analyzed in Clathria reinwardtii was 161.3 mg/kg.
DISTRIBUSI LOGAM ESSENSIAL Cu DALAM SPONGE (PORIFERA) DI KEPULAUAN SPERMONDE Melawaty, Lydia; Noor, Alfian; Harlim, Tjodi; Voogd, Nicole de
Marina Chimica Acta Vol 13, No 2 (2012): Volume 13 No. 2 (2012)
Publisher : Hasanuddin University

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Abstract

A research on essential metal distribution of copper in Spermonde waters has been carried out. Sponge species of Melophlus sarassinorum, Callyspongia aerizusa, dan Clathria reinwardtii were identified and analyzed accordingly. Copper level were measured using Inductrively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) and the results showed that highest concentration was found in Clathria reinwartii  48.3 ppm. Copper distribution in sponge skeleton is higher than in its tissue and seemingly in terms of species the highest concentration is Clathria reinwartii taken from Barrang Lompo waters. In the meantime, Samalona waters contain highest concentration in its sedimen (3.96 ppm). Apparently Spermonde waters is beginning being interferred by metal pollution.
PRODUKSI SENYAWA BAKTERIOSIN SECARA FERMENTASI MENGGUNAKAN ISOLAT BAL Enterococcus faecium DU55 DARI DANGKE Razak, Abd. Rahman; patong, Abd. Rauf; Harlim, Tjodi; Djide, M. Natsir; Haslia, Haslia; Mahdalia, Mahdalia
Indonesia Chimica Acta Volume 2 No 2 - December 2009
Publisher : Indonesia Chimica Acta

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Abstract

Dangke is one of traditional food from Enrekang Province Sulawesi Selatan which is made from buffalo milk and enzimatically processed using papain from papaya’s gland secretion. Research on this local product as source of lactic acid bacteria (LAB) has been done. Counted 30 LAB was successfully isolated and 3 of them were potentially to be able to yield antimicrobial compound. Enterococcus faecium DU55 is one of LAB isolate available to be applied for producing bacteriocin compound through fermentation. Optimum condition of fermentation was specified by determining the highest antimicrobial activity generated by filtrate of fermentation result. Antimicrobial activity examination was carried out through diffusion agar method by measuring inhibition zone growth of pathogen bacterium Salmonella typhimurium FNCC 0050. Research results indicate that maximum bacteriocin compound production by BAL isolate E. faecium DU55 was obtained at condition of optimum fermentation at 30 °C during 42 hour using M1 medium with the same composition to medium MRS (Man Rogosa and Sharpe).Keyword: dangke, LAB isolate, bacteriocin, antimicrobial activity
Skrining Bioaktivitas Beberapa Bagian Jaringan Tumbuhan Paliasa(Melochia umbellata (Hout) Stapf var. Degrabrata K) Erwin, Erwin; Noor, Alfian; Soekamto, Nunuk Hariani; Harlim, Tjodi
Indonesia Chimica Acta Volume 2 No 1 - June 2009
Publisher : Indonesia Chimica Acta

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Abstract

Paliasa is one of tropical plants that used as an etnobotanical traditional drug by the people of South Sulawesi. Paliasa leaves is believed and used as a drug for hypertension, diabetes mellitus, cholesterol, and hepatitis diseases. Preliminary study was done by Brine shrimp lethality test with Artemia salina to a tissue part of one of type paliasa, Melocia umbellata (Houtt.) Stapf. var. degrabrata K. The result show that the wood of this plant is the most active with LC50 value of 1,80 ppm. Keywords : Paliasa, traditional drug, M. umbellata, bioactivity.