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Pengaruh Model Pembelajaran Berbasis Masalah dan Ekspositori dengan Keterampilan Proses Sains Terhadap Hasil Belajar Fisika Siswa Kelas VIII MTsN 1 Mataram Tahun Ajaran 2014/2015 Hardiyanto, Hardiyanto; Susilawati, Susilawati; Harjono, Ahmad
Jurnal Pendidikan Fisika dan Teknologi Vol 1, No 4 (2015): Oktober
Publisher : FKIP Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpft.v1i4.267

Abstract

This research aims to (1) reveal the effect of problem based and expository learning towards physics’ learning outcomes, (2) uncover the effect of students’ science process skill towards physics’ learning outcomes, (3) reveal the interaction between students’ science process skill and their physics’ learning outcomes. This study is a quasi-experimental study applying factorial design 2 x 2. The study was conducted from November 2014 to May 2015.  The population of this research is the whole students of class VIII which comprises of 9 classes in MTS Negeri 1 Mataram. The purposive sampling was chosen as the sampling technique for this study, in which class VIII-8 acted as the experiment class and class VIII-7 as the controlled class. The data collection encompasses data of science process skill, and students’ physics’ learning outcomes (in the cognitive, affective, and psychomotor domains) towards the main material of light. Technique of collecting data of science process and physics’ learning outcomes in affective and psychomotor domains were done by using observation sheet with certain criteria, while cognitive domain was done through the test.  The test instrument has previously been through validity test, reliability test, difficulty level, and discrimination index. The data obtained were firstly tested their normality and homogeneity, then continued by the testing of the hypothesis by using two-way analysis of variance (ANOVA). Data analysis technique was done by using ANOVA 2x2 with different cell. The result of this study shows that (1) problem based and expository learning affects physics’ learning outcomes, (2) students’ science process skill affects their physics’ learning outcomes, and (3) there is no interaction between science process skill and students’ learning outcomes.
Strengthening Weselpos Domestic Product Competitiveness Using Business Model Hardiyanto, Hardiyanto; Hamsal, Mohammad
The Indonesian Journal of Business Administration Vol 2, No 12 (2013)
Publisher : The Indonesian Journal of Business Administration

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Abstract

Financial service business of PT. Pos Indonesia has been showing the significant growth for contribution revenues. The contribution of financial service business to PT. Pos Indonesia revenues is up to 34% at 2011. Financial service business has potential to increase the revenue by its potential large market and its competitive advantage. The revitalization plan of PT. Pos Indonesia explained that financial service business will be increased on the transaction number and on product portfolio. Currently, not all financial service products of PT. Pos Indonesia have equitable growth. Weselpos domestic product is a product which has decreased the number of transactions from year to year. In formulating business strategies, the analysis of business environment is done either externally and internally to get SWOT (Strength, Weakness, Opportunity, and Threat) of the business. The root cause problem is PT. Pos Indonesia is insensitive about business environment change. The root cause problem of the business is addressed using TOWS Matrix strategic formulation and business model to strengthen the strategy with strategic positioning as a guideline. Differentiation in the process of delivering services to consumers is proposed as strategic positioning, which is from human-based become automatic based. The strategies are focused on increasing customer satisfaction and increase customer loyalty. The implementation of the strategy consist of: create new products portfolio auto-service based, develop the existing products through the development of transaction process, making customer relationship management, and create the right marketing program. The implementation is needed one year time period. Keywords: Weselpos Competitiveness, Business Model, Weselpos Product Development, Financial Services
Perbanyakan Massal Embrio Kalamondin Melalui Teknologi Somatik Embriogenesis Menggunakan Bioreaktor Devy, Nirmala Frianti; Yulianti, Farida; Hardiyanto, Hardiyanto
Jurnal Hortikultura Vol 22, No 1 (2012): Maret 2012
Publisher : Indonesian Center for Horticultural Research and Development

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Abstract

Sejauh ini, penelitian perbanyakan somatik embriogenesis baik untuk penyediaan semaian batang bawah maupun varietas komersial jeruk menghasilkan laju multiplikasi yang relatif lambat. Kombinasi antara perbanyakan melalui metode somatik embriogenesis dengan penggunaan bioreaktor, diharapkan mampu meningkatkan laju produksi kalus embrionik menjadi planlet.  Kajian awal dilakukan menggunakan nuselus Kalamondin (Citrus mitis Blanco) sebagai sumber kalus. Kalus yang dihasilkan diinduksi dan diperbanyak menjadi kalus embrionik dan embrio dengan cara dikulturkan pada shaker (100 rpm) serta bulb bioreactor. Tujuan penelitian ini ialah membandingkan produksi embrio Kalamondin melalui teknologi somatik embriogenesis pada kultur cair menggunakan shaker dan bioreaktor. Penelitian dilakukan di Laboratorium Kultur Jaringan Balai Penelitian Tanaman Jeruk dan Buah Subtropika, dari September 2008 sampai dengan Desember 2009.  Pada tahapan perbanyakan embrio dengan metode shaker, diperoleh bahwa rerata kemampuan kalus menghasilkan embrio dalam kultur selama 10 minggu ialah 18,12 embrio/g kalus. Dengan kisaran waktu yang sama, total embrio yang dihasilkan 3 g kalus/300 cc media cair di dalam bioreaktor menghasilkan 46 embrio/g kalus atau setara 2,53 kali dibandingkan metode shaker. Embrio yang tumbuh pada bioreaktor dapat berkembang hampir 100% menjadi planlet. Hasil penelitian ini membuktikan bahwa aplikasi bioreaktor untuk tujuan perbanyakan massal embrio Kalamondin memiliki pengaruh yang signifikan terhadap laju multiplikasinya.ABSTRAKSo far, research on somatic embryogenesis for rootstock and citrus commercial varieties has been faced by low multiplication rate of embryos. Combination of somatic embryogenesis method and bioreactor hypothezed can increase multiplication rate of embryos and improve regeneration of embryogenic calli to produce plantlets.  Kalamondin explants were inducted and proliferated to be embryonic calli and embryos using both shaker (100 rpm) and bulb bioreactor. The aimed of this research was to compare the production of Kalamondin embryos through somatic embryogenesis method on liquid media using shaker and bulb bioreactor. Research was conducted at Tissue Culture Laboratory of Indonesian Citrus and Subtropical Fruit Research Institute from September 2008 to December 2009.  Kalamondin nucelus as a callus source was used in this research. Results of the study indicated that the average of embryos production through shaker technique within 10 weeks of culture incubation was 18.12 embryos/g callus, while application of bioreactor imrpoved embryo productivity up to  46 embryos/g calli (3 g/300 cc media). The multiplication rate using the bioreactor increased up to 2.53 fold compare to shaker method. Results of the study give the real evidence that application of biorector for in vitro mass propagation of Kalamondin embryos had high significant effect on embryo multiplication rate.
Kemampuan Regenerasi Kalus Segmen Akar pada Beberapa Klon Bawang Putih Lokal Secara In Vitro Devy, Friyanti Nirmala; Hardiyanto, Hardiyanto
Jurnal Hortikultura Vol 19, No 1 (2009): Maret 2009
Publisher : Indonesian Center for Horticultural Research and Development

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Abstract

ABSTRAK. Regenerasi tanaman bawang putih dapat dilakukan menggunakan kalus sebagai bahan. Namun metodeini dapat juga digunakan untuk perbanyakan, terutama pada produksi tanaman bebas virus. Tujuan penelitian ialahmemperoleh komposisi media yang sesuai untuk pertumbuhan kalus dan regenerasi beberapa klon bawang putih.Penelitian dilakukan di Laboratorium Kultur Jaringan Balai Penelitian Tanaman Jeruk dan Buah Subtropika mulaiMaret 2005 sampai dengan Agustus 2006. Rancangan yang digunakan adalah acak lengkap faktorial dengan 2perlakuan dan 6 ulangan. Induksi kalus dilakukan pada segmen apikal akar bawang putih yang ditanam secara invitro. Persentase jumlah eksplan yang berkalus cukup tinggi, berkisar antara 70-100% pada media MS+0,2 g/l CH +1 ppm 2.4 D maupun media MS + 1 ppm 2.4 D + 0,1 ppm IAA. Meskipun demikian, hanya 2 klon yang memberikanrespons pertumbuhan dan regenerasi kalus yang lebih baik dibandingkan klon lainnya, yaitu Lumbu Kuning danTawangmangu. Pada fase regenerasi menggunakan media MS + 1 ppm kinetin dan MS + 1 ppm IAA + 10 ppm 2-ip,kalus embrionik dari 2 klon tersebut menghasilkan persentase akar yang paling tinggi, masing-masing sebesar 60dan 70% dengan kisaran jumlah akar/eksplan mencapai 2-6 buah. Jumlah planlet berkisar antara 5-10 buah. Padafase perkembangan selanjutnya umbi mikro terbentuk sempurna.ABSTRACT. Devy, N.F. and Hardiyanto. 2009. Regeneration Capacity of Callus-derived from Root Segmentsof Several Local Garlic Clones. The regeneration of garlic using callus as explants is usually used for breedingprogram such as genetic transformation activities. However, this method can also be used as propagation method,especially for virus-free planting maerials. The experiment was carried out at Tissue Culture Laboratory, IndonesianCitrus and Subtropical Fruit Research Institute from March 2005 to August 2006. The experiment was arranged ina factorial randomized complete design with 2 treatments and 6 replications. The callus induction was derived fromgarlic apical root segment via in vitro. The percentage of total explants that produce callus was very high (70-100%)on both medium MS+0.2 g/l CH + 1 ppm 2,4 D and MS + 1 ppm 2, 4 D + 0.1 ppm IAA. Nevertheless, it was only2 clones that gave better callus growth and regeneration responses than others, these were Lumbu Kuning andTawangmangu. On the subculture medium (MS + 1 ppm kinetin and MS + 1 ppm IAA + 10 ppm 2-ip), the percentageof rooted embryogenic callus of both Lumbu Kuning and Tawangmangu were also high, i.e. 60 and 70% respectivelywith 2-6 roots/explant. Shoots grew as a mass, with total shoots number of 5-10 per a mass. Normal micro bulbletswere produced in the next development phase
THE GROWTH PERFORMANCE OF CITRUS DERIVED FROM SOMATIC EMBRYOGENESIS PLANTLET AND SCION STOCK Devy, Nirmala Friyanti; Yenni, Yenni; Hardiyanto, Hardiyanto
Indonesian Journal of Agricultural Science Vol 15, No 2 (2014): October 2014
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Somatic embryogenesis (SE) of callus culture in vitro is one of citrus propagation ways for producing free virus and genetically true-to-type plantlets. To induce growing of plantlets derived from this technology, they should be grafted ex vitro onto a citrus rootstock. The research aimed to evaluate the growth performance of citrus plants cv. Siam Kintamani (Citrus nobilis L.) that used both plantlets and scions as their stocks. The research was conducted at Tlekung Research Station, Indonesian Citrus and Subtropical Fruit Research Institute from June 2011 to December 2012. The treatments were done at nursery house by grafting a plantlet and budding a scion onto an eight-month-old Japanese Citroon (JC) rootstock plant. The grafted and budded plants of one-year old were maintained at nursery house then transplanted into the field. In the field, the research was arranged in a randomized block design with three replications and used 15 plants as unit samples. The results showed that the vegetative growth of Siam Kintamani seedling derived from SE or grafted plant was faster than that of budded plant started from 10 to 12 months after treatment in the nersery house. In the field, the growth of SE grafted plant was only significantly different up to 6 months after transplanting. Plantlets produced from SE in vitro propagation can be used as a good alternative stock material for producing healthy citrus plants. Therefore, a further research is required especially on varieties used, reproductive growth and massive planlets production.
Pembungaan Jeruk Kalamondin Hasil Perbanyakan Melalui Somatik Embriogenesis yang Disambung pada Batang Bawah JC Devy, Nirmala Friyanti; Hardiyanto, Hardiyanto; Yulianti, Farida
Jurnal Hortikultura Vol 23, No 1 (2013): Maret 2013
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v23n1.2013.p21-27

Abstract

Fase vegetatif mencakup fase juvenil yang ditandai dengan munculnya percabangan, pertumbuhan duri, serta belum berkembangnya bunga. Karakter ini ditemukan pada periode vegetatif asal biji dan hasil perbanyakan somatik embriogenesis (SE). Tujuan penelitian ialah mengetahui kemampuan berbunga dan berbuah tanaman jeruk Kalamondin hasil perbanyakan SE yang disambung dengan batang bawah JC setelah 1 tahun ditanam di lapangan. Penelitian pembungaan pada tanaman hasil perbanyakan SE yang disambung dengan batang bawah JC dilakukan di Kebun Percobaan Tlekung, Balai Penelitian Tanaman Jeruk dan Buah Subtropika, pada Bulan Februari 2011-Maret 2012. Tanaman jeruk Kalamondin berasal dari hasil sambungan ex vitro, yaitu batang atas berasal dari embrio kotiledonari dan planlet disambungkan pada batang bawah JC dengan tiga perlakuan, yaitu planlet JC hasil perbanyakan SE yang berumur 4 dan 8 bulan setelah aklimatisasi serta semaian biji umur 8 bulan. Tanaman jeruk Kalamondin hasil sambungan berumur 1 tahun, ditanam di lapangan dan disusun secara RAK dengan tiga ulangan dengan unit percobaaan tiga tanaman. Hasil penelitian menunjukkan bahwa sampai dengan umur 7 bulan di lapangan, tanaman masih pada fase vegetatif, dengan pertumbuhan tertinggi pada perlakuan KPS yaitu tanaman yang berasal dari planlet Kalamondin yang disambungkan pada semaian JC. Namun, pada bulan kedelapan setelah tanam, pertanaman menunjukkan fase generatif yang ditandai dengan munculnya organ bunga. Jumlah bunga dan buah tertinggi terdapat pada perlakuan tanaman yang berasal dari planlet Kalamondin yang disambungkan pada batang bawah JC hasil aklimatisasi. Dari penelitian ini disimpulkan bahwa hasil perbanyakan jeruk melalui SE, berupa embrio kotiledonari maupun planlet dapat dimanfaatkan sebagai batang atas yang tumbuh dan berkembang dengan normal di lapangan apabila didukung oleh kondisi lingkungan yang optimal.
THE GROWTH PERFORMANCE OF CITRUS DERIVED FROM SOMATIC EMBRYOGENESIS PLANTLET AND SCION STOCK Devy, Nirmala Friyanti; Yenni, Yenni; Hardiyanto, Hardiyanto
Indonesian Journal of Agricultural Science Vol 15, No 2 (2014): October 2014
Publisher : Indonesian Center for Agricultural Library Technology Dissemination - IAARD

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v15n2.2014.71-78

Abstract

Somatic embryogenesis (SE) of callus culture in vitro is one of citrus propagation ways for producing free virus and genetically true-to-type plantlets. To induce growing of plantlets derived from this technology, they should be grafted ex vitro onto a citrus rootstock. The research aimed to evaluate the growth performance of citrus plants cv. Siam Kintamani (Citrus nobilis L.) that used both plantlets and scions as their stocks. The research was conducted at Tlekung Research Station, Indonesian Citrus and Subtropical Fruit Research Institute from June 2011 to December 2012. The treatments were done at nursery house by grafting a plantlet and budding a scion onto an eight-month-old Japanese Citroon (JC) rootstock plant. The grafted and budded plants of one-year old were maintained at nursery house then transplanted into the field. In the field, the research was arranged in a randomized block design with three replications and used 15 plants as unit samples. The results showed that the vegetative growth of Siam Kintamani seedling derived from SE or grafted plant was faster than that of budded plant started from 10 to 12 months after treatment in the nersery house. In the field, the growth of SE grafted plant was only significantly different up to 6 months after transplanting. Plantlets produced from SE in vitro propagation can be used as a good alternative stock material for producing healthy citrus plants. Therefore, a further research is required especially on varieties used, reproductive growth and massive planlets production.
Pengaruh Densitas Awal Kalus dalam Perbanyakan Melalui Embriogenesis Somatik terhadap Daya Multiplikasi dan Stabilitas Genetik Planlet Siam Kintamani Devy, Nirmala Friyanti; Yulianti, F; Hardiyanto, Hardiyanto
Jurnal Hortikultura Vol 22, No 4 (2012): Desember 2012
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v22n4.2012.p309-315

Abstract

Optimasi metode pada setiap tahapan perbanyakan melalui embriogenesis somatik perlu dilakukan, mencakup aspek eksplan, media, dan lingkungan tumbuh.  Tujuan penelitian ialah mengetahui pengaruh kepadatan awal (initial density) kalus dalam kultur embriogenesis somatik terhadap laju multiplikasi dan stabilitas genetik planlet yang dihasilkan dari perbanyakan dengan metode SE pada tanaman siam Kintamani. Penelitian dilaksanakan di Laboratorium SE, Balai Penelitian Tanaman Jeruk dan Buah Subtropika (Balitjestro) mulai Bulan Maret 2009 sampai dengan Februari 2011. Penelitian terdiri atas dua tahap, yaitu (1) perlakuan densitas awal dan (2) analisis stabilitas genetik planlet yang dihasilkan dari perbanyakan SE  siam Kintamani. Kegiatan I terdiri atas lima perlakuan densitas kalus (ID100–ID300), yaitu  100, 150, 200, 250, dan 300 mg yang dikulturkan pada 25 ml media cair MS + 500 mg/l malt ekstrak (ME) + 1,5 mg/l BA, yang disusun dalam rancangan acak kelompok dengan tiga ulangan, tiap ulangan terdiri atas lima erlenmeyer, sedangkan pada penelitian analisis stabilitas genetik, sampel yang digunakan ialah tanaman hasil perbanyakan SE pada stadia planlet hasil subkultur 1–6. Planlet tersebut diuji keragamannya dengan teknik PCR menggunakan penanda intersimple sequence repeat (ISSR). Hasil penelitian menunjukkan bahwa, jaringan nuselus yang digunakan sebagai eksplan dapat tumbuh dengan memuaskan pada 12–45 hari setelah kultur pada media inisiasi kalus.  Pertambahan berat basah kalus pada setiap subkultur sangat beragam.  Pertambahan berat basah tertinggi terjadi pada ID100 subkultur ke-5, sedangkan pertambahan berat secara total tertinggi ditemukan pada perlakuan ID200. Tanaman hasil perbanyakan SE pada stadia planlet secara genetik seragam dengan induknya. Namun pengujian stabilitas genetik pada tanaman hasil SE masih harus terus dilakukan seiring dengan semakin lama tanaman dipelihara di dalam kultur, mengingat frekuensi mutasi dapat meningkat seiring dengan semakin lamanya periode kultur. Implikasi hasil penelitian ini ialah proses multiplikasi kalus dan induksi embriogenesis somatik berlangsung optimal dan tidak mengakibatkan off-type pada tanaman yang dihasilkan
Perbanyakan Massal Embrio Kalamondin Melalui Teknologi Somatik Embriogenesis Menggunakan Bioreaktor Devy, Nirmala Frianti; Yulianti, Farida; Hardiyanto, Hardiyanto
Jurnal Hortikultura Vol 22, No 1 (2012): Maret 2012
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v22n1.2012.p1-7

Abstract

Sejauh ini, penelitian perbanyakan somatik embriogenesis baik untuk penyediaan semaian batang bawah maupun varietas komersial jeruk menghasilkan laju multiplikasi yang relatif lambat. Kombinasi antara perbanyakan melalui metode somatik embriogenesis dengan penggunaan bioreaktor, diharapkan mampu meningkatkan laju produksi kalus embrionik menjadi planlet.  Kajian awal dilakukan menggunakan nuselus Kalamondin (Citrus mitis Blanco) sebagai sumber kalus. Kalus yang dihasilkan diinduksi dan diperbanyak menjadi kalus embrionik dan embrio dengan cara dikulturkan pada shaker (100 rpm) serta bulb bioreactor. Tujuan penelitian ini ialah membandingkan produksi embrio Kalamondin melalui teknologi somatik embriogenesis pada kultur cair menggunakan shaker dan bioreaktor. Penelitian dilakukan di Laboratorium Kultur Jaringan Balai Penelitian Tanaman Jeruk dan Buah Subtropika, dari September 2008 sampai dengan Desember 2009.  Pada tahapan perbanyakan embrio dengan metode shaker, diperoleh bahwa rerata kemampuan kalus menghasilkan embrio dalam kultur selama 10 minggu ialah 18,12 embrio/g kalus. Dengan kisaran waktu yang sama, total embrio yang dihasilkan 3 g kalus/300 cc media cair di dalam bioreaktor menghasilkan 46 embrio/g kalus atau setara 2,53 kali dibandingkan metode shaker. Embrio yang tumbuh pada bioreaktor dapat berkembang hampir 100% menjadi planlet. Hasil penelitian ini membuktikan bahwa aplikasi bioreaktor untuk tujuan perbanyakan massal embrio Kalamondin memiliki pengaruh yang signifikan terhadap laju multiplikasinya.ABSTRAKSo far, research on somatic embryogenesis for rootstock and citrus commercial varieties has been faced by low multiplication rate of embryos. Combination of somatic embryogenesis method and bioreactor hypothezed can increase multiplication rate of embryos and improve regeneration of embryogenic calli to produce plantlets.  Kalamondin explants were inducted and proliferated to be embryonic calli and embryos using both shaker (100 rpm) and bulb bioreactor. The aimed of this research was to compare the production of Kalamondin embryos through somatic embryogenesis method on liquid media using shaker and bulb bioreactor. Research was conducted at Tissue Culture Laboratory of Indonesian Citrus and Subtropical Fruit Research Institute from September 2008 to December 2009.  Kalamondin nucelus as a callus source was used in this research. Results of the study indicated that the average of embryos production through shaker technique within 10 weeks of culture incubation was 18.12 embryos/g callus, while application of bioreactor imrpoved embryo productivity up to  46 embryos/g calli (3 g/300 cc media). The multiplication rate using the bioreactor increased up to 2.53 fold compare to shaker method. Results of the study give the real evidence that application of biorector for in vitro mass propagation of Kalamondin embryos had high significant effect on embryo multiplication rate.
Pengaruh Media dan Penyungkupan Terhadap Daya Tumbuh Benih Jeruk Bebas Penyakit Hasil Penyambungan Meristem Tip In Vitro Devy, Nirmala Friyanti; Suhariyono, Suhariyono; Hardiyanto, Hardiyanto
Jurnal Hortikultura Vol 25, No 1 (2015): Maret 2015
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v25n1.2015.p15-25

Abstract

Teknologi penyambungan meristem tip (PMT) untuk mengeliminasi penyakit yang berasal dari graft-transmissible pada tanaman jeruk di Indonesia telah dilakukan sejak dua dekade lalu. Namun tingkat pertumbuhan benih hasil penyambungannya masih sangat rendah dan waktu yang diperlukan untuk proses indeksing masih relatif panjang. Penelitian dilaksanakan di Laboratorium Kultur Jaringan dan Rumah Pembibitan Balai Penelitian Tanaman Jeruk dan Buah Subtropika mulai Januari 2012 – Maret 2013. Tujuan penelitian ini adalah mendapatkan teknologi PMT yang diperbarui melalui modifikasi media in vitro serta penyungkupan untuk meningkatkan persentase keberhasilan dan daya tumbuh tanaman. Perlakuan yang diaplikasikan adalah penambahan konsentrasi vitamin MS pada media persemaian biji batang bawah jeruk Japanese Citroen/JC (Citrus medica) dan media pertumbuhan tanaman keprok Batu 55 (Citrus reticulata Blanco) hasil PMT in vitro. Pada tahapan berikutnya, perlakuan yang diaplikasikan adalah perbaikan kondisi lingkungan tumbuh berupa penambahan ukuran polibag dan pengaturan suhu lingkungan mikro tanaman dengan melakukan penyungkupan menggunakan plastik tanaman hasil PMT yang di-regrafting. Penelitian dirancang dalam rancangan acak lengkap dengan lima ulangan pada tahapan pertumbuhan semai in vitro, rancangan acak lengkap faktorial dengan lima ulangan pada pertumbuhan tanaman hasil PMT, dan rancangan acak kelompok faktorial dengan tiga ulangan pada tahapan regrafting. Hasil penelitian menunjukkan bahwa  pada fase semaian batang bawah in vitro, penambahan konsentrasi vitamin pada media sampai 10 kali tidak berpengaruh nyata terhadap persen perkecambahan biji serta pertumbuhan semaian JC, sedangkan pada fase ex vitro, perlakuan kombinasi antara ukuran polibag dan penyungkupan tidak berpengaruh nyata terhadap semua parameter yang diamati. Perlakuan penyungkupan pada minggu ke–10 sampai dengan ke–14 berpengaruh nyata terhadap peningkatan pertumbuhan tinggi dan jumlah daun jeruk keprok Batu 55, masing-masing mencapai 40,1% dan 25,9%. Hasil penelitian ini akan mempercepat kisaran waktu yang dibutuhkan untuk menghasilkan tanaman induk jeruk bebas penyakit dengan menggunakan teknik PMT.