Dian Hardianto
Balai Besar Pengembangan Budidaya Air Tawar Sukabumi

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PENERAPAN SELEKSI FAMILI F3 PADA IKAN NILA HITAM (Oreochromis niloticus) Yuniarti, Tristiana; Hanif, Sofi; Hardianto, Dian
SAINTEK PERIKANAN Vol 4, No 2 (2009): Jurnal Saintek Perikanan
Publisher : SAINTEK PERIKANAN

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Abstract

ABTSRAKPemuliaan ikan nila di Indonesia merupakan kegiatan perekayasaan yang sangat penting dilakukan untuk menigkatkan mutu genetik ikan nila yang ada di masyarakat. Metode Seleksi Famili telah digunakan sebagai satu metode efektif untuk mendapatkan strain induk nila yang lebih unggul. Pada tahun 2005 telah berhasil membuat generasi pertama seleksi famili sebanyak 35 famili, tahun 2006 telah menghasilkan 49 famili dan tahun 2007 menghasilkan 39 famili. Masing-masing famili terdiri dari dua sub populasi yaitu induk jantan dan induk betina. Jumlah populasi hasil seleksi pada sub populasi jantan dan betina masing-masing dapat memenuhi jumlah top grad minimal 15 ekor jantan dan 15 ekor betina. Jumlah famili yang memijah 39 famili. Hasil cut off pada masing-masing sub famili mempunyai bobot rataan 120,14+7,3 g pada sub populasi jantan dan 97,36 + 2,6 g pada sub populasi betina. Mutu genetik yang diperoleh pada generasi F3 menghasilkan nilai heritabiliti sebesar 0,142 dengan respon seleksi 25,4 g. Proses seleksi masih perlu dilanjutkan kepada generasi ke-4 untuk memperoleh generasi yang lebih unggul.Kata Kunci: Tilapia, program pemijahan, seleksi familiABSTRACTGenetic improvement of tilapia in Indonesia is very important in order to improve the tilapia quality. Family selection method was known as an effective method to get higher quality brood stock. In 2005, 35 first generation of families selection were successfully done, in 2006, 49 families were produced and in 2007, 39 families were produced. Each family consisted of two sub population, i.e. male and female brood stocks. The number of male and female sub population selection could fulfill the top grade minimal number of 15 males and 15 females. 39 families spawned. The cut off results of each sub family had average body weight of 120.14 ± 7.3 g for male sub population and 97.36 ± 2.6 g for female sub population. The genetic quality which was obtained on F3 generation had a heritability value of 0.142 with selection respond 25.4 g. The selection process was still needed to be continued on F4 generation to get better quality generationKey words: Tilapia, breeding program, family selection
The sex ratio and testosterone levels in tilapia immersed in different doses of 17α-methyltestosterone Junior, Muhammad Zairin; Naufal, Muhammad Restya; Setiawati, Mia; Hardianto, Dian; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol 16, No 1 (2017): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.16.1.51-59

Abstract

  Tilapia fish farming using monosex male population has been reported to have 10% higher productivity compared to mix-sex culture. This study aimed to determine immersion dose of 17?-metiltestosterone (MT) that allowed higher male percentage, growth, survival, and measure testosterone level in fish body. The experiment was designed as three immersion doses, namely: 0; 1.8; and 5.4 mg/L MT, each with three replications. Immersion was conducted to 14-day-old larvae for four hours, with density of 100 fish/L of water. Testosterone levels in fish was measured using ELISA method, and sex identification was performed by histology method. The result showed that percentage of male fish was the same in the treatment of 1.8 mg/L and 5.4 mg/L, and 53?65% higher than the control without MT treatment (54% male). Growth and survival of fish until day 56 post immersion were the same. By ELISA analysis, the levels of testosterone in larvae just after immersion was similar in 1.8 mg/L and 5.4 mg/L treatments, decreased drastically on day 14 after immersion, and then the levels of testosterone to be similar with the control on day 28, i.e. about 2 ng/g. By PCR method with specific primer, sex reversed and normal males could be distinguished, and on day 71 the testosterone levels in sex reversed and normal males fish was also the same, 0.3 ng/g (P>0.05). Thus, sex reversal by immersion at a dose of 1.8 mg/L can be consider as a standard protocol for monosex tilapia production. Testosterone level in the body of MT-treated fish becomes the same to the control of less than one month post immersion
Genetic variability of the fifth generation of nile tilapia Oreochromis niloticus using microsatellite DNA markers Satriani, Gloria Ika; Soelistyowati, Dinar Tri; Hardianto, Dian; Aliah, Ratu Siti
Jurnal Akuakultur Indonesia Vol 10, No 2 (2011): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.10.124-130

Abstract

ABSTRACTFifth generations of Nile tilapia from several strains have been produced by using selective breeding program in Main Centre for Freshwater Aquaculture Development (MCFAD) Sukabumi, West Java. This research was aimed to evaluate the impact of family selection program of some highly economic traits on its genetic variability using microsatellite DNA markers. The total of 180 specimens have been collected from fifth generation of nine reciprocal mating between three families selected from fourth generation of Nile tilapia and were screened for genetic variability at three microsatellite loci (UNH 123*, UNH 172*, UNH 216*). The results showed that the amount of genetic variability on fifth generations of Nile tilapia from three strains was ranged between 33 to 100% and the highest genetic distance relationship between families was 0.3875. This research approved that females and males issued from the family which have more amount of genetic variability and higher distance to others could be considered as genetic materials to produce the next generation.Keywords: microsatellite DNA, genotype, genetic variability, genetic distance, Oreochromis nilotiocus ABSTRAKBeberapa strain ikan nila generasi kelima telah dihasilkan dalam program pemuliaan di Main Centre untuk Freshwater Aquaculture Development (MCFAD) Sukabumi, Barat Jawa. Riset ini bertujuan untuk mengevaluasi pengaruh seleksi famili terhadap performa karakter ekonomis penting berdasarkan keragaman genetiknya menggunakan penanda microsatellite DNA. Spesimen dari 180 individu generasi kelima hasil persilangan resiprokal antara tiga famili generasi keempat dianalisis dengan penanda tiga microsatellite loci (UNH 123*, UNH 172*, UNH 216*). Hasil penelitian menunjukkan bahwa keragaman genetik ikan nila generasi kelima berkisar antara 33 sampai 100% dan hubungan kekerabatan genetik antar famili yang paling jauh adalah 0,3875. Individu betina dan jantan yang berasal dari famili dengan tingkat keragaman genetik dan kekerabatan yang lebih tinggi dapat dipertimbangkan sebagai sumber genetik berkualitas untuk menghasilkan generasi berikutnya.Kata kunci: microsatellite DNA, genotipe, keragaman genetik, jarak genetik, Oreochromis nilotiocus