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Journal : Sriwijaya Journal of Environment

Genetic Relatedness Of Local Varieties Of Rice South Sumatra Based On Polymerase Chain Reaction – Random Amplified Polymorphic DNA (PCR-RAPD) Hanum, Laila; Windusari, Yuanita; Muharni, Muharni; Adriansyah, Fikri
Sriwijaya Journal of Environment Vol 2, No 1 (2017): Green Environment For Human Welfare
Publisher : Program Pascasarjana Universitas Sriwijaya

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Abstract

Diversity 22 local varieties of rice South Sumatra has the potential to be developed into rice varieties. The similarity (similarity) among 22 local varieties of rice South Sumatra became one of the obstacles for scientists to develop rice varieties. One solution to this problem is to determine the genetic relatedness between 22 rice local vaietas South Sumatra. Identification of genetic relatedness among 22 local varieties of rice South Sumatra can be carried out using PCR-RAPD. This study aims to determine the phylogenetic relationship among 22 local varieties of rice South Sumatra. This research about DNA isolation and visualitation has been carried out in August 2015 until December 2015 in Microbiology Laboratory and Laboratory of Genetics and Biotechnology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya and research about DNA quality and quantity test and PCR-RAPD has been done in Biotechnology Labotory Faculty of Mathematics and Natural Sciences, Sriwijaya University. Obtained 22 local varieties of rice accessions South Sumatra. Genetic relatedness identified using PCR-RAPD technique. 7 primer (OPA, OPA-9, OPA-10, OPA-13, OPA-16, OPA-19, OPB-8) was used for amplification. Local varieties of rice South Sumatra is divided into two major groups in association coefficient of 0.76, namely groups A and B. Group A consists of groups A1 and A2 in association coefficient of 0.78. In the large group B also consists of two groups: the B1 and B2 in association coefficient of 0.84. Local varieties of rice genetic relatedness South Sumatra are not influenced by geographical location.Keywords: Local Varieties Of Rice South Sumatra, Genetic Relatedness,  PCR-RAPD
Diversity and Kinship of the Swamp Buffalo (Bubalus bubalis) from Pampangan South Sumatra Based On Morphological Characteristics Windusari, Yuanita; Hanum, Laila; Pratama, Rahmat
Sriwijaya Journal of Environment Vol 1, No 3 (2016): Biodiversity
Publisher : Program Pascasarjana Universitas Sriwijaya

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Abstract

Abstract: Previous studies have found four variant of swamp buffalo in the region of Pampangan based on the characteristics of habitats and morphology namely black buffalo, red,belang, and Lampung. Hence done observation to know the diversity and the kinship relation based on morphological characteristics. A method of on farm was done to data characterization and morphology. Data was analyzed using NTSys ver.2.1 and presented in dendrogram. Cluster analysis done with un-weighted pair-grup method with arithmetic averaging (UPGMA) with a coefficient similarities. The results show that morphology between variant buffalo that in eye color, the color of body, body size, or shapes and sizes horns. Buffalo belang having a dark eyes, the body white ribbed and light. Buffalo black having a black eyes and black body. Buffalo red having a red eyes, the body a red light colored (blonde). Buffalo Lampung allegedly is introduce from Lampung area, adapt and married with a local buffalo. The red buffalo having red eyes, like a black buffalo but shorter size with curved horns the way down. The results of the analysis kinship based on morphological characteristics show buffalo black and buffalo Lampung allegedly came from a single characterized by value a correlation coefficient of 0,85. Inbreeding and adaptation factors believed to cause different the phenotype and morphology. Buffalo  red having scarlet kinship the lowest is as much as 0,57 and predicted to have an ancestor different. Based on these results can be expressed variant swamp buffalo of regional Pampangan tending to low and the difference in the phenotype of influenced inbreeding and adaptation to the environmentKeywords: diversity, kinship, swamp buffalo (Bubalus bubalis), morphological characteristics, Pampangan South SumatraAbstrak : Berdasarkan karakteristik habitat dan pengamatan terhadap morfologi telah ditemukan empat varian kerbau rawa di wilayah Pampangan yaitu kerbau hitam, merah, belang, dan Lampung.  Untuk mengetahui keanekaragaman dan hubungan kekerabatan antar varian kerbau dilakukan penelitian ini. Pengamatam lapangan ditujukan untuk memperoleh data mengenai karakterisasi dan morfologi. Data dianalisis menggunakan NTSYS Ver.2.1 dan disajikan dalam bentuk dendrogram. Analisis kluster menggunakan metode Unweighted pair-grup method with arithmetic averaging (UPGMA) untuk mendapatkan koefisien similaritas. Hasil  menunjukkan perbedaan morfologi antar varian kerbau terlihat pada warna mata, warna tubuh, ukuran tubuh, serta bentuk dan ukuran tanduk.  Kerbau rawa belang memiliki mata gelap, tubuh bergaris putih dan ukuran tubuh kecil; kerbau hitam memiliki mata hitam dan warna kulit hitam;  kerbau mata merah memiliki mata berwarna tubuh lebih terang (warna bulu pirang); sedangkan kerbau Lampung diduga berasal dari daerah Lampung, yang beradaptasi dan kawin dengan kerbau lokal. Kerbau merah memiliki mata berwarna merah, memiliki tanduk yang lebih pendek dan melengkung ke bawah. Hasil analisis terhadap kekerabatan antar varian kerbau rawa diketahui bahwa kerbau hitam dan kerbau Lampung diperkirakan berasal dari satu induk dengan nilai koefisien korelasi sebesar 0,85. Inbreeding dan adaptasi diyakini menjadi faktor munculnya beberapa fenotip dan morfologi. Nilai kekerabatan terendah sebesar 0,57 ditemukan pada varian kerbau merah dan diperkirakan berasal dari nenek moyang berbeda. Berdasarkan hasil tersebut disimpulkan bahwa keragaman genetik dari kerbau rawa yang berasal dari daerah  Pampangan relatif rendah dan perbedaan fenotip lebih dipengaruhi oleh inbreeding dan adaptasi lingkungan.Kata kunci: keragaman, kekerabatan, kerbau rawa (Bubalus bubalis), karakteristik morfologi, Pampangan Sumatera Selatan