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Karakter Makromorfologi dan Mikromorfologi Duku, Kokosan, Langsat dalam Penentuan Status Taksonomi pada Kategori Infraspesies Hanum, Laila; Kasiamdari, Rina Sri; Santosa, Santosa; Rugayah, Rugayah
Biospecies Vol 6, No 02 (2013)
Publisher : Biospecies

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Abstract

Duku, kokosan dan langsat merupakan buah-buahan yang populer dan memiliki nilai ekonomi yang penting. Secara morfologi duku, kokosan dan langsat  hampir mirip satu sama lain sehingga menyulitkan dalam penentuan pada tingkat infraspesies bagi para pemulia tanaman. Berbagai pendekatan untuk penentuan morfologi ini telah banyak dilakukan. Tujuan penelitian ini menentukan status taksonomi duku, kokosan dan langsat pada ketegori infraspesies berdasarkan karakteritik makro dan mikromorfologi. Berbagai variasi duku, langsat dan kokosan dikoleksi dari beberapa pusat pertanaman di Indonesia, meliputi wilayah Sumatera, Jawa dan Kalimantan. Preparasi herbarium tumbuhan meliputi koleksi sampel, persiapan, penempelan dan identifikasi. Analisis morfologi menggunakan metode pengamatan dan deskripsi, pengukuran karakter morfologi dilakukan baik pada organ generatif dan vegetatif meliputi bunga, batang, biji dan buah, dan semua sampel didokumentasikan dengan menggunakan foto. Hasil penelitian ini menunjukkan ditemukan 29 variasi dari duku, kokosan, dan langsat yang terdiri dari 19 duku, 2 kokosan dan 8 langsat dari semua wilayah. Hasil karakterisasi makro dan mikromorfologi menunjukkan duku, kokosan, dan langsat dari berbagai daerah di Indonesia dapat dinyatakan duku, kokosan, dan langsat merupakan marga Lansium, untuk kategori jenis adalah L. domesticum Correa dan pada kategori infraspesies dapat dibagi menjadi dua group yaitu group duku dan group kokosan-langsat.
Genetic Relatedness among Duku, Kokosan, and Pisitan in Indonesia Based on Random Amplified Polymorphic DNA Markers Hanum, Laila; Kasiamdari, Rina Sri; ., Santosa; ., Rugayah
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

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Abstract

Genetic relatedness among duku, kokosan, and pisitan from Indonesia were investigated using random amplified polymorphic DNA (RAPD) markers. Eleven primers (OPA-01, OPA-02, OPA-10, OPB-07, OPB-11, OPB-12, OPB-15, OPT-16, OPU-14, OPU-19, and OPU-20) were used for amplification and yielded a total of 174 DNA bands, of which 167 were polymorphic. Primer OPA-10, OPB-11, OPB-12, OPB-15, and OPU-19 produced all of the polymorphic DNA bands. The size of the amplified DNA fragments ranged from 41-1546 bp. The dendrogram separated into two clusters at a genetic similarity coefficient of 0.76. The cluster 1 consisted of subclusters duku and several pisitan (pisitan OKI, pisitan Sleman, pisitan Hatu, pisitan Punggur, and pisitan Tanjung), and cluster 2 consisted of subclusters kokosan and pisitan. In the kokosan subclusters, including duku Drendan. Dendrogram supported the determination of taxonomic status of duku, kokosan, and pisitan as one species, namely Lansium domesticum Corr. and its divided into two groups, namely L. domesticum ’duku group’ and L. domesticum ’pisitan-kokosan group’. Thus, RAPD analysis was useful tool for determining the genetic variation and the genetics relatedness among duku, kokosan, and pisitan in Indonesia.Key words: duku, kokosan, pisitan/langsat, genetic relatedness, RAPD
The Phylogenetic Relationship Among Varieties of Lansium domesticum Correa Based on ITS rDNA Sequences Hanum, Laila; Kasiamdari, Rina Sri; ., Santosa; ., Rugayah
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

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Abstract

Lansium domesticum Corr. with vernacular name in Indonesian duku has been reported containingtherapeutic bioactive compounds, and some of these compounds shown to be potent antitumor, anticancer,antimalaria, antimelanogenesis, antibacteria, and antimutagenic activities. This plant is commonly known asduku, kokosan and langsat by the local community in Indonesia. The morphological appearance of all varieties isnearly the same, and identifi cation of the varieties is very diffi cult for growers. Variation of DNA sequences ofthe ITS (Internal transcribed spacer) region can be used as a molecular character to determine the phylogeneticrelationship of different varieties of L. domesticum. The aims of this study were to determine taxonomy status ofduku, kokosan, and langsat, also phylogenetic relationship among varieties of L. domesticum based on ITS rDNAsequencing. DNA was isolated from leaves of plant and then amplifi ed using F1 and R1 primers. Nucleotidesequences were identifi ed using Sequence Scanner Software Programm version 1.0, nucleotide sequences from18S, ITS1, 5.8S, ITS2 and 26S region, that has been mergered using EditSeq and SegMan in software Suite forSequence Analysis DNASTAR Lasergene DM version 3.0.25. The results of study showed that DNA fragmentsranging in size from 782-810 bp. Different pattern of DNA fragments indicated polymorphism among duku,kokosan, and langsat. Based on the results of the ITS rDNA sequencing and phylogenetic tree analysis. Itwas determined that Lansium and Aglaia are a separated genus with the similarity index value of 0.98. Duku,kokosan and langsat were divided into two cluster, namely cluster kokosan-langsat and cluster duku with thesimilarity index value of 0.996.Keywords : Phylogenetic relationship, ITS region, L. domesticum, duku, kokosan, langsat
THE DEVELOPMENT OF INVENTORY, MONITORING AND INFORMATION NETWORKS SYSTEM OF FAUNAL DIVERSITY IN SOUTH SUMATRA Yustian, Indra; Setiawan, Arum; Setiawan, Doni; Hanum, Laila; Dahlan, Zulkifli
BIOVALENTIA: Biological Research Journal Vol 3, No 2 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

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Abstract

Despite high attention and commitment of Indonesia in biodiversity conservation and its habitat, however, loss of biodiversity and habitat deforestation is also still high. Deforestation rate in Indonesia between period of 2000-2012 reach up 6.02 million ha, and approximately onethird of which is in Sumatra Island. As the fulfillment of CBD and Aichi targets, and in line with the mandate of the Law on the Conservation of Natural Resources and Ecosystems and IBSAP (Indonesian Biodiversity Strategy & Action Plan 2015-2020), we try to support the requires data as well as information on the current state of biodiversity. The aims are to develop an inventory system of biodiversity needed to establish baseline data on biodiversity including its conservation status in South Sumatra, develop a biodiversity monitoring system with qualitative parameters that can be used in the determination and monitoring of degradation rates of biodiversity, and the establishment of data management system and information network of fauna biodiversity in South Sumatera so that it can be utilized in monitoring and reporting of biodiversity at regional, national and international level at the international level. We conduct a comprehensive review of methods and techniques of inventory and monitoring of fauna biodiversity, especially those that have been done in the area of South Sumatra Province. We also analysis of data needs and information network of fauna biodiversity in South Sumatra. The results is shows in the web-application database, called the South Sumatran Biodiversity Information Networks, or SSBIN, and could preview at http://ssbin.unsri.ac.id/.Keywords: south sumatra, biodiversity, information, networks, SSBIN.
Genetic Relatedness Of Local Varieties Of Rice South Sumatra Based On Polymerase Chain Reaction – Random Amplified Polymorphic DNA (PCR-RAPD) Hanum, Laila; Windusari, Yuanita; Muharni, Muharni; Adriansyah, Fikri
Sriwijaya Journal of Environment Vol 2, No 1 (2017): Green Environment For Human Welfare
Publisher : Program Pascasarjana Universitas Sriwijaya

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Abstract

Diversity 22 local varieties of rice South Sumatra has the potential to be developed into rice varieties. The similarity (similarity) among 22 local varieties of rice South Sumatra became one of the obstacles for scientists to develop rice varieties. One solution to this problem is to determine the genetic relatedness between 22 rice local vaietas South Sumatra. Identification of genetic relatedness among 22 local varieties of rice South Sumatra can be carried out using PCR-RAPD. This study aims to determine the phylogenetic relationship among 22 local varieties of rice South Sumatra. This research about DNA isolation and visualitation has been carried out in August 2015 until December 2015 in Microbiology Laboratory and Laboratory of Genetics and Biotechnology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya and research about DNA quality and quantity test and PCR-RAPD has been done in Biotechnology Labotory Faculty of Mathematics and Natural Sciences, Sriwijaya University. Obtained 22 local varieties of rice accessions South Sumatra. Genetic relatedness identified using PCR-RAPD technique. 7 primer (OPA, OPA-9, OPA-10, OPA-13, OPA-16, OPA-19, OPB-8) was used for amplification. Local varieties of rice South Sumatra is divided into two major groups in association coefficient of 0.76, namely groups A and B. Group A consists of groups A1 and A2 in association coefficient of 0.78. In the large group B also consists of two groups: the B1 and B2 in association coefficient of 0.84. Local varieties of rice genetic relatedness South Sumatra are not influenced by geographical location.Keywords: Local Varieties Of Rice South Sumatra, Genetic Relatedness,  PCR-RAPD
Diversity and Kinship of the Swamp Buffalo (Bubalus bubalis) from Pampangan South Sumatra Based On Morphological Characteristics Windusari, Yuanita; Hanum, Laila; Pratama, Rahmat
Sriwijaya Journal of Environment Vol 1, No 3 (2016): Biodiversity
Publisher : Program Pascasarjana Universitas Sriwijaya

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Abstract

Abstract: Previous studies have found four variant of swamp buffalo in the region of Pampangan based on the characteristics of habitats and morphology namely black buffalo, red,belang, and Lampung. Hence done observation to know the diversity and the kinship relation based on morphological characteristics. A method of on farm was done to data characterization and morphology. Data was analyzed using NTSys ver.2.1 and presented in dendrogram. Cluster analysis done with un-weighted pair-grup method with arithmetic averaging (UPGMA) with a coefficient similarities. The results show that morphology between variant buffalo that in eye color, the color of body, body size, or shapes and sizes horns. Buffalo belang having a dark eyes, the body white ribbed and light. Buffalo black having a black eyes and black body. Buffalo red having a red eyes, the body a red light colored (blonde). Buffalo Lampung allegedly is introduce from Lampung area, adapt and married with a local buffalo. The red buffalo having red eyes, like a black buffalo but shorter size with curved horns the way down. The results of the analysis kinship based on morphological characteristics show buffalo black and buffalo Lampung allegedly came from a single characterized by value a correlation coefficient of 0,85. Inbreeding and adaptation factors believed to cause different the phenotype and morphology. Buffalo  red having scarlet kinship the lowest is as much as 0,57 and predicted to have an ancestor different. Based on these results can be expressed variant swamp buffalo of regional Pampangan tending to low and the difference in the phenotype of influenced inbreeding and adaptation to the environmentKeywords: diversity, kinship, swamp buffalo (Bubalus bubalis), morphological characteristics, Pampangan South SumatraAbstrak : Berdasarkan karakteristik habitat dan pengamatan terhadap morfologi telah ditemukan empat varian kerbau rawa di wilayah Pampangan yaitu kerbau hitam, merah, belang, dan Lampung.  Untuk mengetahui keanekaragaman dan hubungan kekerabatan antar varian kerbau dilakukan penelitian ini. Pengamatam lapangan ditujukan untuk memperoleh data mengenai karakterisasi dan morfologi. Data dianalisis menggunakan NTSYS Ver.2.1 dan disajikan dalam bentuk dendrogram. Analisis kluster menggunakan metode Unweighted pair-grup method with arithmetic averaging (UPGMA) untuk mendapatkan koefisien similaritas. Hasil  menunjukkan perbedaan morfologi antar varian kerbau terlihat pada warna mata, warna tubuh, ukuran tubuh, serta bentuk dan ukuran tanduk.  Kerbau rawa belang memiliki mata gelap, tubuh bergaris putih dan ukuran tubuh kecil; kerbau hitam memiliki mata hitam dan warna kulit hitam;  kerbau mata merah memiliki mata berwarna tubuh lebih terang (warna bulu pirang); sedangkan kerbau Lampung diduga berasal dari daerah Lampung, yang beradaptasi dan kawin dengan kerbau lokal. Kerbau merah memiliki mata berwarna merah, memiliki tanduk yang lebih pendek dan melengkung ke bawah. Hasil analisis terhadap kekerabatan antar varian kerbau rawa diketahui bahwa kerbau hitam dan kerbau Lampung diperkirakan berasal dari satu induk dengan nilai koefisien korelasi sebesar 0,85. Inbreeding dan adaptasi diyakini menjadi faktor munculnya beberapa fenotip dan morfologi. Nilai kekerabatan terendah sebesar 0,57 ditemukan pada varian kerbau merah dan diperkirakan berasal dari nenek moyang berbeda. Berdasarkan hasil tersebut disimpulkan bahwa keragaman genetik dari kerbau rawa yang berasal dari daerah  Pampangan relatif rendah dan perbedaan fenotip lebih dipengaruhi oleh inbreeding dan adaptasi lingkungan.Kata kunci: keragaman, kekerabatan, kerbau rawa (Bubalus bubalis), karakteristik morfologi, Pampangan Sumatera Selatan
Genetic Relatedness among Duku, Kokosan, and Pisitan in Indonesia Based on Random Amplified Polymorphic DNA Markers Hanum, Laila; Kasiamdari, Rina Sri; Santosa, S.; Rugayah, R.
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Original Source | Check in Google Scholar | Full PDF (306.302 KB)

Abstract

Genetic relatedness among duku, kokosan, and pisitan from Indonesia were investigated using random amplified polymorphic DNA (RAPD) markers. Eleven primers (OPA-01, OPA-02, OPA-10, OPB-07, OPB-11, OPB-12, OPB-15, OPT-16, OPU-14, OPU-19, and OPU-20) were used for amplification and yielded a total of 174 DNA bands, of which 167 were polymorphic. Primer OPA-10, OPB-11, OPB-12, OPB-15, and OPU-19 produced all of the polymorphic DNA bands. The size of the amplified DNA fragments ranged from 41-1546 bp. The dendrogram separated into two clusters at a genetic similarity coefficient of 0.76. The cluster 1 consisted of subclusters duku and several pisitan (pisitan OKI, pisitan Sleman, pisitan Hatu, pisitan Punggur, and pisitan Tanjung), and cluster 2 consisted of subclusters kokosan and pisitan. In the kokosan subclusters, including duku Drendan. Dendrogram supported the determination of taxonomic status of duku, kokosan, and pisitan as one species, namely Lansium domesticum Corr. and its divided into two groups, namely L. domesticum ’duku group’ and L. domesticum ’pisitan-kokosan group’. Thus, RAPD analysis was useful tool for determining the genetic variation and the genetics relatedness among duku, kokosan, and pisitan in Indonesia.Key words: duku, kokosan, pisitan/langsat, genetic relatedness, RAPD
The Phylogenetic Relationship Among Varieties of Lansium domesticum Correa Based on ITS rDNA Sequences Hanum, Laila; Kasiamdari, Rina Sri; Santosa, S.; Rugayah, R.
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Original Source | Check in Google Scholar | Full PDF (208.799 KB)

Abstract

Lansium domesticum Corr. with vernacular name in Indonesian duku has been reported containingtherapeutic bioactive compounds, and some of these compounds shown to be potent antitumor, anticancer,antimalaria, antimelanogenesis, antibacteria, and antimutagenic activities. This plant is commonly known asduku, kokosan and langsat by the local community in Indonesia. The morphological appearance of all varieties isnearly the same, and identifi cation of the varieties is very diffi cult for growers. Variation of DNA sequences ofthe ITS (Internal transcribed spacer) region can be used as a molecular character to determine the phylogeneticrelationship of different varieties of L. domesticum. The aims of this study were to determine taxonomy status ofduku, kokosan, and langsat, also phylogenetic relationship among varieties of L. domesticum based on ITS rDNAsequencing. DNA was isolated from leaves of plant and then amplifi ed using F1 and R1 primers. Nucleotidesequences were identifi ed using Sequence Scanner Software Programm version 1.0, nucleotide sequences from18S, ITS1, 5.8S, ITS2 and 26S region, that has been mergered using EditSeq and SegMan in software Suite forSequence Analysis DNASTAR Lasergene DM version 3.0.25. The results of study showed that DNA fragmentsranging in size from 782-810 bp. Different pattern of DNA fragments indicated polymorphism among duku,kokosan, and langsat. Based on the results of the ITS rDNA sequencing and phylogenetic tree analysis. Itwas determined that Lansium and Aglaia are a separated genus with the similarity index value of 0.98. Duku,kokosan and langsat were divided into two cluster, namely cluster kokosan-langsat and cluster duku with thesimilarity index value of 0.996. Keywords : Phylogenetic relationship, ITS region, L. domesticum, duku, kokosan, langsat
Bioacumulation of cadmium (Cd) Within water around the final solid waste disposal (FWD) of Sukawinatan Palembang Julius, Deni; Suheryanto, Suheyanto; Hanum, Laila
Indonesian Journal of Environmental Management and Sustainability Vol 2 No 3 (2018): September
Publisher : ARTS Publishing

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Abstract

There has been research on the Bioacumulation of Cadmium in the Leachate, Sediment, Sepat fish and watercress within water around the final solid waste disposal (FWD) of Sukawinatan Palembang using Atomic Absorption Spectrophotometric (SSA) technique. This study aims to determine the distribution of cadmium contained in water ecosystem within water around the final solid waste disposal (FWD) of Sukawinatan, as well as bioconcentration factor (BCF) of the biotic compartement to the abiotic compartement . The way is done by wetdestruction sample. The resultant destruction solution was analyzed at a wavelength of 228.8 nm. The results showed that the concentration of cadmium in leachate Inlet 0,0099 mg/L until the Sedapat River 0,0010 mg/L, Sediment Inlet 0,0427 mg/Kg until Sedapat River Sediment 0,0051 mg/Kg, Sepat fish reservoir 0,0099 mg/Kg and Sepat fish Sedapat River 0,0096 mg/Kg along watercress reservoir 0,0042 mg/Kg over Sedapar river 0,0027 mg/Kg. So that distribution of cadmium for water and sediment compartment getting further from leachate inlet to Sedapat river is decreasing. The value of cadmium consentration in leachate is still below the threshold conformable in regulation of environment and forestry ministers no.59 of 2016 is 0,1 mg/L. Distribution  on the fish and watercress compartment also descreased to the Sedapat river. Consentration value on the fish and watercress also stil below the quality standard  in this SNI 7378;2009 for fish 0,1 mg/Kg and watercress 0,2 mg/Kg. While the value of BCF fish and BCF watercress  <100 is still in low category.
Analisis Polimorfisme Padi Varietas Lokal Sumatera Selatan Berdasarkan Pendekatan PCR-RAPD Adriansyah, Fikri; Hanum, Laila; Muharni, Muharni; Windusari, Yuanita
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol 7, No 1 (2018): JLSO
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Abstract

Adriansyah et al, 2018. Polymorphism Analysis of Local Varieties of South Sumatra Rice Based on PCR-RAPD. JLSO 7(1):50-58.The existence of rice germplasm is threatened because of the introduction of various high-yielding varieties. This study aimed to analyze rice kinship and conservation of local varieties of South Sumatra rice. This study was conducted in August 2015 to December 2015. DNA isolation using the Promega Wizard Purification Systems KIT. This study uses OPA-9 primer, OPA-10, OPA-13, OPA-16. All primers can amplify DNA with optimal quality. OPA-9 primers produced 5 DNA bands, OPA-10 primers produced 9 DNA bands, OPA-13 primers produced 12 DNA and Primary OPA-16 bands producing 9 ppita DNA.