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Journal : Medical Journal of Indonesia

Polymorphisms in the pfcrt and pfmdr1 genes in Plasmodium falciparum isolates from South Sumatera, Indonesia Saleh, Irsan; Handayani, Dwi; Anwar, Chairil
Medical Journal of Indonesia Vol 23, No 1 (2014): February
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Background: Over the past decade, antimalarial drug resistance has rapidly become a major public health problem in South East Asia region including South Sumatra. This study aimed to determine the extent of gene polymorphisms associated with chloroquine resistance (CQR) in P. falciparum isolates from Lahat, Sekayu, Baturaja and Palembang district.Methods: A molecular study was conducted to identify the mutant alleles of the genes associated with the resistance to chloroquine among the isolates of Plasmodium falciparum from South Sumatera. Blood from 25 patients was collected, DNA was isolated, and the sequences of two different genes (Plasmodium falciparum chloroquine resistance transporter/pfcrt and Plasmodium falciparum multidrug resistance/pfmdr1) were analyzed using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).Results: This study identified polymorphism in the pfcrt 76-Thr in all isolates and pfmdr1 86-Tyr. These findings may reflect the failure of treatment with the standard dose of chloroquine within the last few years in South Sumatera. Conclusion: PCR-RFLP technique provide a simple and rapid method of detecting polymorphisms in genes that may predict chloroquine resistance (CQR). Although the identification of the polymorphism in the pfcrt and pfmdr1 genes provides a significant indicator of CQR, further studies are needed to determine the role of these polymorphisms in the in vivo and in vitro responses to drug treatment.Keywords: chloroquine, Plasmodium falciparum, pfmdr1, pfcrt
Co-Authors . Wahyuni, . Aat Sriati Abdul Mun’im Ahmad Ghiffari Ana Sutrisnawati, Ana Anindya Ardiansari Anna S. Ranti, Anna Ardiayasari, Anindya Aribowo, Luita Arifianto, Indriyana Putri Armina, Armina Avin F Helmi, Avin F Ayu, Friska Bertha M Sopha, Bertha M Budi Hartono Chairil Anwar Chatarina Umbul W, Chatarina Dalilah Dalilah Dewi, Novita Puspita Diyono Ikhsan Dwiputra, Kemas Muhammad Alwan Efri Widianti Ella Amalia, Ella Elva Nuraina Endang Sutisna Sulaeman Eti Poncorini Pamungkasari Fahmi Arifan Fani, Reisa Hesti Fifa Argentina FS Nugraheni S Haris Wibisono Haryanto, Didid I.G.B. Budi Dharma, I.G.B. Budi Iche Andriyani Liberty, Iche Andriyani Indah Fitri Nurdianthi, Indah Fitri Indah Hartati Irsan Saleh Kuara Jangga Uma, Wilhelmus Laila Faizah Achmad, Laila Faizah Laila Faizah, Laila Lina D. Fathimahhayati, Lina D. Lisdiana - Lusia Hayati, Lusia Lutfi, Mochtar M K Herliansyah, M K Maghfiroh Rahayu Nindatama, Maghfiroh Rahayu Margaretha TS, Margaretha Mohammad Endy Yulianto Muhaimin Ramdja, Muhaimin Muhammad Ilyas Muhammad Reza Murni Murni Nova Kumalasari Novera Herdiani, Novera Nurhidayah Nurhidayah Nurul Asiah Oktavian, Rosian Pangestu, Ilyas Teguh Petricka, Gracea Prameswarie, Thia Prasasti, Gita Dwi Prasasty, Gita Dwi Pratiwi Puji Lestari, Pratiwi Puji Puji, Pratiwi Rico Vendamawan Rizki Amy Lavita, Rizki Amy Santi Martini Sari, Septa Satriya Nurgiri, Yoga Selly Pinangki, Selly Septiana, R. A. Leni Sianipar, Melpa Yohana Siti Harnina Bintari Siti Latifah Soegiyanto Soegiyanto, Soegiyanto Sri Rustiyaningsih Suhendrianto Suhendrianto, Suhendrianto Sukamto, Neubrina Raesuky Suryadi, Kresna Theresia Purbandari Tri Rustiadi, Tri Utama, Denny Satria Veronika Prasetya Vita Paramita Wahyu Sopandi Yahya, Yulia Farida Yulastini, Fitria