Iwan Sahrial Hamid
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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Hambatan Ekspresi Vascular Endothelial Growth Factor oleh Ekstrak Daun Sambung Nyawa pada Endotel Membran Korioalantois (EFFECTIVITY OF SAMBUNG NYAWA LEAF EXTRACT TO INHIBIT VASCULAR ENDOTHELIAL GROWTH FACTOR EXPRESSION ON ENDOTHELIALS OF CHORIOALLANTOIC Hamid, Iwan Sahrial; Nazar, Dady Soegianto; Ratnani, Hermin
Jurnal Veteriner Vol 14, No 1 (2013)
Publisher : Jurnal Veteriner

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Abstract

Angiogenesis is the new blood vessels formation normality and important on growth and developmentof individu. Angiogenesis also have contribution to carcinogenesis or uncontrolled and malignant cancercell development, become pathologic condition like inflammatory and infection. The purpose of this researchfor knew the effectivities of Gynura procumbens extract on various dose for inhibition Vascular EndothelialGrowth Factor (VEGF) expression. This research was done to effort cancer progress inhibition. However,angiogenesis is part of carcinogenesis causes. The Chorio Allantoic Membrane (CAM) methods was usedfor this aim. Eggs at the age of nine days were divided into 6 groups. Group I were negative control ofvehicle, group II were zero treatment: 60 ng bFGF which aplicated into paper dish. The next four groupswere extract of Gynura procumbens that divided in four dose: 60, 75, 90 and 110 µg + bFGF 60 ng whichapplicated into  paper dish.  At the twelve days old, VEGF expression analysis was done whichimunohystochemical method with anti VEGF’s antibody. The result of this research showed that therewas significant different (p<0.05) on give of Gynura procumbens extract to VEGF expression. The mostsignificant VEGF inhibition by Gynura procumbens extract with dose 110 µg. The conclusion on this studywas Gynura procumbens extract effective to inhibit the VEGF expression on CAM embrio chick.
Pengaruh Pemberian Ekstrak Kedelai (Glycine max) Terhadap Jumlah Pertumbuhan Folikel Ovarium Mencit (Mus musculus) Pramesti, Nugraheni Anggara; Restiadi, Tjuk Imam; Yudhana, Aditya; Hernawati, Tatik; Hamid, Iwan Sahrial; Purnama, Muhammad Thohawi Elziyad
Jurnal Medik Veteriner Vol 1, No 3 (2018): Jurnal Medik Veteriner
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol1.iss3.2018.120-127

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Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak kedelai (Glycine max) terhadap jumlah pertumbuhan folikel ovarium pada mencit (Mus musculus). Sampel terdiri dari 20 ekor mencit yang secara acak dibagi menjadi lima kelompok. Kelompok kontrol (K) tidak diberi ekstrak kedelai. Kelompok P1 diberi ekstrak kedelai dengan dosis 0,05 mg/kgBB. Kelompok P2 diberi ekstrak kedelai dengan dosis 0,010 mg/kgBB. Kelompok P3 diberi ekstrak kedelai dengan dosis 0,015 mg/kgBB. Kelompok P4 diberikan ekstrak kedelai dengan dosis 0,020 mg/kgBB. Ekstrak kedelai diberikan selama 14 hari. Analisis data menggunakan uji One Way ANOVA dan dilanjutkan dengan uji Honestly Significant Difference (HSD). Hasil penelitian menunjukkan kelompok kontrol (K) memiliki perbedaan signifikan dengan kelompok P1, P2, P3 dan P4. Hasil rata-rata jumlah folikel primer tertinggi terdapat pada perlakuan 4 (P4) dengan dosis 0,020 mg/kgBB dan jumlah rata-rata folikel sekunder tertinggi pada perlakuan 3 (P3) dengan dosis 0,015 mg/kgBB. Penelitian ini dapat disimpulkan bahwa ekstrak kedelai yang diberikan pada mencit betina dapat meningkatkan jumlah pertumbuhan folikel ovarium dan jumlah rata-rata folikel tertinggi adalah pada perlakuan 3 (P3) dengan dosis 0,015 mg/kgBB.
The Number of Macrophages and Heterophils on Chick Embryo Chorioallantoic Membrane After Gynura procumbens (Lour) Merr Extract Treatment and bFgF Induction Hamid, Iwan Sahrial; P, Yuseni Kusuma; Bijanti, Retno; Aksono, E Bimo
Indonesian Journal of Cancer Chemoprevention Vol 3, No 2 (2012)
Publisher : Indonesian Research Gateway

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Abstract

Antiangiogenesis (inhibition of new blood vessels formation) has become a strategy to inhibit cancer development. The aim of this experiment was to investigate antiangiogenic effect  of  Gynura  procumbens  (Lour)  Merr  focusing  on  the  decreasing  of  the  number  of macrophages  and  heterophils  on  chick  embryo  chorioallantoic  membrane.  Nine-days-aged-eggs  were  divided  into  six  groups  (eight  eggs  each  group).  Group  I  (positive  control)  eggs were  induced  with  bFGF+Tris  HCl.  Group  II  (negative  control)  eggs  were  treated  with DMSO+Tris  HCl.  Group  III  (treatment  I)  eggs  were  induced  with  60  ng  bFGF  and  treated with  ethanolic  extract  of  G.  procumbens  leaves  with  the  dose  of  60  µg.  The  following treatment  groups,  i.e.  group  IV  (treatment  II),  group  V  (treatment  III),  and  group  VI (treatment IV) were treated with increasing dose of extract, starting from 75 µg, 90 µg, and the last was 110 µg. Eggs were incubated until they reach the age of twelve days to observe macrophages, while  to observe heterophils, eggs were incubated until  the  age of seventeen days. Based on haematoxylin-eosin staining, macrophages in the treatment groups were less than the control positive group (bFGF+Tris HCl), but based on giemsa staining, the effect of Gynura procumbens  in decreasing the number of heterophils could not  be observed  because some blood smears. These analysis suggest that  the  ethanolic extract of  Gynura procumbens leaves can perform as antiangiogenic agent decreasing the number of macrophages.Keywords: antiangiogenic, macrophages, heterophils, Gynura procumbens
CYP1A1 and GSTm expression of hepatocytes induced by 7,12-dimethylbenz(a)anthracene and the influence of ethanolic extract of Gynura procumbens Hamid, Iwan Sahrial; ., Sugiyanto; Meiyanto, Edy; Widyarini, Sitarina
INDONESIAN JOURNAL OF PHARMACY Vol 20 No 4, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.093 KB) | DOI: 10.14499/indonesianjpharm0iss0pp198-206

Abstract

The cytochrome P-450 (CYP) and glutathione S-transferase (GST) enzyme systems may influence the biological effects of carcinogens. As such, these enzymes may predict the developmental risk of breast cancer, as well as be potential targets for chemoprevention. The purpose of this study was to compare the expression of CYP1A1 and GST* between treatment groups. Expression of CYP1A1 and GST* was quantified in liver tissue from 18 female Sprague Dawley rats aged 40 days, which randomly divided into six treatment groups. Those are base line group (without DMBA and ethanolic extract treatment), DMBA induced cancer group, the other two groups was administrated by DMBA after treated by ethanolic extract in two different doses, 300 mg /kg BW and 750 mg/kg BW. The last two groups were given two doses of extract ethanolic only, without initiated of DMBA. The ingestion of the extract was carried for three weeks and the ingestion of DMBA was performed twice in the third week. The expression CYP1A1 and GST was quantified by immunohistochemistry. CYP1A1 expression was significantly higher (P < 0.05) in cancer group (DMBA) as compared with other groups. On the other hand, GST expression was lower in cancer group (P < 0.05). Result of this study demonstrated that ethanolic extract leaves of G. procumbens in 300 mg/kg BW dose could inhibit CYP1A1 stronger than are other and induced GST* level. Has effect on ethanolic extract leaves of G. procumbens has ability in played role of as blocking agent in preventing initiation stage of carsinogenesis, therefore it should be taken into account for chemopreventing agent in mammary carsinogenesis.Key words : Gynura procumbens, breast cancer, Chemopreventive, CYP1A1, GSTμ
Ethyl p-methoxycinnamate from Kaempferia galanga inhibits angiogenesis through tyrosine kinase Ekowati, Juni; Hardjono, Suko; Hamid, Iwan Sahrial
Universa Medicina Vol 34, No 1 (2015)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2015.v34.43-51

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BACKGROUNDMany tumors express on their receptor tyrosine kinases vascular endothelialgrowth factor activity associated with angiogenesis. Inhibition ofangiogenesis through reduction of tyrosine kinase activity is a promisingstrategy for cancer therapy. The present study aimed to determine themechanism and potency of ethyl p-methoxycinnamate (EPMC) isolatedfrom Kaempferia galanga as angiogenesis inhibitor.METHODSA laboratory experimental study was conducted using chorio-allantoicmembranes (CAMs) of nine-day old chicken eggs induced by 60ng basicfibroblast growth factor (bFGF). Ethyl p-methoxycinnamate (EPMC) potencywas determined at dosages of 30, 60, 90 and 120 μg and compared withcelecoxib 60 μg as reference drug and one negative bFGF-induced controlgroup. Neovascularization and endothelial cell count in CAM blood vesselswere evaluated. To predict the antiangiogenic mechanism of EPMC, adocking study was performed with the Molegro Virtual Docker program ontyrosine kinase as receptor (PDB 1XKK).RESULTSAngiogenesis stimulation by bFGF was prevented significantly (p<0.05)by EPMC at dosages of 30, 60, 90 and 120 μg and this activity was dosedependent. Molecular docking showed interaction between EPMC functionalgroups and tyrosine kinase amino acids at Met766, Met793, Thr854, Thr790,Gln791 and Ala743. There was an association between EPMCantiangiogenic activity and docking study results.CONCLUSIONSEthyl p-methoxycinnamate is a potential new angiogenesis inhibitor throughinteraction with tyrosine kinase. EPMC could be a promising therapeuticagent for treatment of angiogenesis-related diseases.
Hidroponik Fodder Jagung sebagai Substitusi Hijauan Pakan Ternak Ditinjau dari Produktivitas Susu Kambing Sapera Saputro, Amung Logam; Hamid, Iwan Sahrial; Prastiya, Ragil Angga; Purnama, Muhammad Thohawi Elziyad
Jurnal Medik Veteriner Vol 1, No 2 (2018): Jurnal Medik Veteriner
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol1.iss2.2018.48-51

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Hidroponik adalah sistem bercocok tanam menggunakan media cair dan nutrisi-nutrisi yang diperlukan untuk pertumbuhan tanaman. Keuntungan bercocok tanam dengan hidroponik yakni tanaman tidak merusak tanah, tidak membutuhkan tempat yang luas, tanpa pestisida atau obat hama yang merusak tanah, mempercepat pertumbuhan dan menjaga kualitas tanaman. Penelitian ini menggunakan perbandingan konsentrat dan fodder jagung yakni P1 (100:0), P2 (60:40), P3 (75:25) dan P4 (0:100). Hasil penelitian menunjukan bahwa kuantitas susu pada hari ke-1, ke-2 dan ke-3 menunjukkan P4 berbeda nyata (p<0,05) dengan P1, P2, dan P3. Hal ini menunjukkan penurunan kuantitas susu pada P4 dan kuantitas susu terbanyak pada P1 dan P3. Simpulan penelitian perlakuan pemberian fodder jagung 25% dan konsentrat 75% serta perlakuan konsentrat 100% dapat meningkatkan kuantitas susu kambing Sapera.
Uji Organoleptis, pH, Uji Eber dan Cemaran Bakteri pada Karkas yang Diisolasi dari Kios di Banyuwangi Fikri, Faisal; Hamid, Iwan Sahrial; Purnama, Muhammad Thohawi Elziyad
Jurnal Medik Veteriner Vol 1, No 1 (2017): Jurnal Medik Veteriner
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol1.iss1.2017.23-27

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Food borne disease merupakan isu bagi dunia kesehatan karena masih belum bisa dikendalikan secara tuntas. Daging merupakan salah satu agen tempat berkembangnya mikroba infeksius, cemaran kimia dan fisik yang memungkinkan timbulnya food poisoning. Penanganan masalah food borne disease melalui penatalaksanaan penyediaan pemotongan melalui Rumah Potong Hewan (RPH) menjadi salah satu kunci untuk mengendalikan bahan pangan. Kualitas daging menjadi prioritas penting dalam menjamin mutu pangan. Penelitian menggunakan kajian cross sectional study dengan metode sampling purposive. Pengujian sampel di wilayah Kecamatan Banyuwangi, Genteng dan Kalibaru menjadi subjek utama dimana terdapat kios daging yang mensuplai kebutuhan konsumen sehingga akan diketahui kualitas daging yang dipotong melalui RPH. Penelitian ini menunjukkan hasil uji organoleptis warna 37% kelainan, bau normal, tekstur konsistensi keras 0,05%, uji pH 50% diatas ambang normal, uji Eber 45% positif mengalami awal kebusukan. Semua sampel daging negative Salmonella spp dan 50% melebihi ambang batas Standart Nasional Indonesia cemaran Escherichia coli.
Efektivitas Sugar Dressing (100% Gula) dalam Meningkatkan Kepadatan Kolagen pada Proses Penyembuhan Luka Bakar Buatan pada Kulit Tikus Putih (Rattus norvegicus) Jantan Ivanalee, Angela Swasti; Yudaniayanti, Ira Sari; Yunita, Maya Nurwartanti; Triakoso, Nusdianto; Hamid, Iwan Sahrial; Saputro, Amung Logam
Jurnal Medik Veteriner Vol 1, No 3 (2018): Jurnal Medik Veteriner
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol1.iss3.2018.134-141

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Luka bakar merupakan hilangnya atau rusaknya keutuhan kulit akibat paparan suhu atau pH, zat kimia, gesekan, trauma tekanan dan radiasi. Proses penyembuhan luka tidak lepas dari peran kolagen. Sintesis kolagen dapat dipromosikan melalui pemberian gula (sugar dressing). Tujuan penelitian ini adalah mengetahui efektivitas sugar dressing untuk meningkatan kepadatan kolagen dalam proses penyembuhan luka bakar buatan pada kulit tikus putih (Rattus norvegicus) jantan. Dua puluh ekor tikus putih (Rattus norvegicus) jantan dibagi menjadi lima kelompok dengan jumlah empat ekor tiap kelompok. Kelompok K- (kontol negatif) tidak diberi perlukaan. Kelompok K+ (kontrol positif) di beri perlukaan kemudian hanya diberi bilasan NaCl fisiologis. Kelompok P1 (Bioplacenton®) diberi perlukaan dengan pengobatan Bioplacenton®. Kelompok P2 (sugar dressing) diberi perlukaan dengan pengobatan gula dan P3 (madu) menggunakan madu sebagai obat. Perawatan diberikan setiap hari sekali selama empat belas hari. Hasil uji nonparametrik Kruskal-Wallis menunjukkan perbedaan yang signifikan (p<0,05) dan dilanjutkan dengan uji beda Mann-Whitney. Kepadatan kolagen kelompok P1, P2, dan P3 tidak berbeda secara nyata (p>0,05), tetapi berbeda nyata dengan kelompok K- dan K+. Kesimpulan dari penelitian ini adalah sugar dressing efektif untuk meningkatkan kepadatan kolagen pada proses penyembuhan luka bakar.
Deteksi Antibodi Brucella pada Sapi Perah di Kecamatan Purwoharjo Kabupaten Banyuwangi dengan Metode Rose Bengal Test (RBT) Dwi, Welyanto Kurniawan; Tyasningsih, Wiwiek; Praja, Ratih Novita; Hamid, Iwan Sahrial; Sarudji, Suryanie; Purnama, Muhammad Thohawi Elziyad
Jurnal Medik Veteriner Vol 1, No 3 (2018): Jurnal Medik Veteriner
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol1.iss3.2018.142-147

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Penelitian ini bertujuan mengetahui keberadaan antibodi dari bakteri brucella pada sapi perah di Kecamatan Purwoharjo Kabupaten Banyuwangi menggunakan Rose Bengal Test (RBT). Metode yang digunakan dalam penelitian ini adalah metode analisis diskriptif dengan pendekatan kuantitatif, yaitu metode yang menggambarkan keberadaan antibodi Brucella pada sapi perah dengan cepat. Informasi pendukung diperoleh dari survei peternak dengan teknik wawancara. Sampel ditentukan dengan rumus slovin dengan ukuran sampel sebanyak 75 sampel dari 294 ekor sapi pada populasi sapi perah di Kecamatan Purwoharjo. Hasil penelitian menunjukan 2 sampel dinyatakan positif Brucella dari 75 sampel. Hasil penelitian ini dapat disimpulkan bahwa reaktor Brucella ditemukan pada sapi perah di Kecamatan Purwoharjo Kabupaten Banyuwangi yang berpotensi menyebabkan penyakit Brucellosis. Harapan dari penelitian ini adalah data yang diperoleh dapat diuji ulang dan diuji lebih lanjut dalam penelitian selanjutnya sehingga hasil dapat dikonfirmasi.
Identifikasi Escherichia coli dan Salmonella spp pada Karkas Sapi di Rumah Potong Hewan di Banyuwangi dan Resistensi Terhadap Antibiotika Fikri, Faisal; Purnama, Muhammad Thohawi Elziyad; Saputro, Amung Logam; Hamid, Iwan Sahrial
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.22146/jsv.38439

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Food borne disease can be transmitted through Escherichia coli and Salmonella spp contamination. The contamination of microorganisms with high pathogenic potentials on bovine carcasses results in food borne illness. The aim of this study was to identify multidrug-resistance of Escherichia coli and Salmonella spp on carcass samples that isolated from slaughter house in Banyuwangi. Samples were collected from district of Banyuwangi, Rogojampi, Genteng and Kalibaru. This study used cross sectional study with assumption of prevalence at 50% in each contaminant, confidence level 95% and standart of error at 10%. By the number of samples should reach 96 samples. The result showed that seven samples (7,3%) were positive Escherichia coli and none samples (0%) were positive Salmonella spp. The multidrug-resistance of Escherichia coli showed that Cephalotin (42,9%), Trimethoprim (14,3%) and Erythromycin (42,9%) whereas Ampicillin, Enrofloxacin, Chloramphenicol and Tetracycline were sensitive against Escherichia coli.