Helmy Hamid
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Pathogenicity and immunogenicity local isolat infectious laryngo tracheitis virus Indriani, Risa; Hamid, Helmy; Adjid, R.M Abdul; Saepulloh, Muharam
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 2 (2004): JUNE 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.531 KB) | DOI: 10.14334/jitv.v9i2.418

Abstract

Infectious laryngotracheitis (ILT) is an acute and contagious respiratory diseases of chicken. The virus is Gallid herpes and belong to family herpesviridae. Two local strains of ILT virus those were BGR-6 and BKS-3 were isolated and their pathogenicity and immunogenicity were further observed after five time pareses on coris allantoic of specific pathogenic free embryonated eggs. The pathogenicity of both isolates to be possible for use as seed vaccine were detected based on pathogenicity indices and antibody response. Experimental specific pathogenic free chicken in isolator cages were infected by the isolates using103EID50. ILT virus per dose. Clinical syndromes, pathological anatomic lesions, and immunological response were observed in the infected chickens and another group at uninfected chickens as a control. Results showed that either BGR-6 or BKS-3 caused clinical signs with ITPI scores of 0,05 and 0,03 respectively and there were no mortality of infected chickens. The top antibody responces of BGR-6 and BKS-3 were observed at OD 0.90 and 0.44 respectively. It can be concluded that BGR-6 and BBS-3 had low ITPI scores, but BGR-6 gave higher antibody response and can be used as a candidate for seed vaccine.   Key words: Infectious laryngotracheitis, ILT, BGR-6, BKS-3, pathogenicity, immunogenicity
The isolation of canine parvovirus and pathological changes of infected dogs Sendow, Indrawati; Hamid, Helmy
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 1 (2004): MARCH 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.024 KB) | DOI: 10.14334/jitv.v9i1.427

Abstract

The aims of this study are to isolate canine parvovirus (CPV) from the field case and to evaluate its histological aspects in CPV infected dogs. Samples were collected from intestine, intestine contained and mucose, as well as dogs feses from diarrhoea and blood diarrhoea. The suspension of those specimen was inoculated into Feline Kidney cells and observed for cythopathic effect (CPE). The presence of CPE indicated that there was viral isolate and continued to further identification using Haemaglutination (HA) test with pig red blood cells. Samples with positif in HA test were further identification using Haemaglutination Inhibition (HI) test against reference CPV antisera. Isolation result showed that from 81 samples processed, 10 samples indicated CPE in cell cultures, and had agglutinated in pig red blood cells and neutralised reference CPV antisera as the same titer of reference CPV antisera. Nine isolates were obtained from feces and 1 isolate was obtained from Mucose intestine from bloody diarrhoea dogs. Those isolates were also obtained from 1 to 2 days post blood diarrhea clinical signs. Two from 10 cadavers examined showed histological changes to CPV infection. Isolate of CPV, originally from feces, was also obtained from one of the two cadavers. Based on the results it was concluded that more CPV viral isolates can be obtained from bloody diarrhea feces.   Key words: Canine parvovirus, inhibition, isolation, pathology, identification
Mareks Disease in Chicken: Clinico-Pathological Aspect and Diagnosis Damayanti, Rini; Hamid, Helmy
Indonesian Bulletin of Animal and Veterinary Sciences Vol 12, No 2 (2002)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/wartazoa.v12i2.770

Abstract

Marek’s is a limphoproliferative and oncogenic disease of poultry caused by a serotype 1 herpesvirus.  Chicken  between 10 and 15 weeks of age are the most susceptible although in some outbreaks it also affects other ages. The causal agent consists of  some  virus  strains  with  different  pathotypes  causing  a  variety  of  clinical  and  pathological manifestations. The  main pathological features are neurological lesion and lymphoma formation in some visceral organs. Marek’s disease is clinically classified as classical, acute, peracute and chronic with neurological disorder and superficial tumor. At necropsy the peripheral nerves were enlarged, discoloured and loss of striation. There were formation of lymphoid tumour in various visceral organs. Microscopically Marek’s produced non suppurative encephalitis/neuritis and pleomorphic tumour. The diagnosis is based on the history of the disease, age of affected chicken, clinical and pathological features. A definitive diagnosis is made by virological and serological tests. Some of the Marek’s cases in Indonesia were discussed in this paper, as well as review on clinico- pathological aspects how to confirm the diagnosis of Marek’s.   Key words. Marek’s Disease, chicken, clinical, pathological, diagnosis
Pathogenicity and immunogenicity local isolat infectious laryngo tracheitis virus Indriani, Risa; Hamid, Helmy; Adjid, R.M Abdul; Saepulloh, Muharam
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 2 (2004)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v9i2.418

Abstract

Infectious laryngotracheitis (ILT) is an acute and contagious respiratory diseases of chicken. The virus is Gallid herpes and belong to family herpesviridae. Two local strains of ILT virus those were BGR-6 and BKS-3 were isolated and their pathogenicity and immunogenicity were further observed after five time pareses on coris allantoic of specific pathogenic free embryonated eggs. The pathogenicity of both isolates to be possible for use as seed vaccine were detected based on pathogenicity indices and antibody response. Experimental specific pathogenic free chicken in isolator cages were infected by the isolates using103EID50. ILT virus per dose. Clinical syndromes, pathological anatomic lesions, and immunological response were observed in the infected chickens and another group at uninfected chickens as a control. Results showed that either BGR-6 or BKS-3 caused clinical signs with ITPI scores of 0,05 and 0,03 respectively and there were no mortality of infected chickens. The top antibody responces of BGR-6 and BKS-3 were observed at OD 0.90 and 0.44 respectively. It can be concluded that BGR-6 and BBS-3 had low ITPI scores, but BGR-6 gave higher antibody response and can be used as a candidate for seed vaccine.   Key words: Infectious laryngotracheitis, ILT, BGR-6, BKS-3, pathogenicity, immunogenicity
The isolation of canine parvovirus and pathological changes of infected dogs Sendow, Indrawati; Hamid, Helmy
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 1 (2004)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v9i1.427

Abstract

The aims of this study are to isolate canine parvovirus (CPV) from the field case and to evaluate its histological aspects in CPV infected dogs. Samples were collected from intestine, intestine contained and mucose, as well as dogs feses from diarrhoea and blood diarrhoea. The suspension of those specimen was inoculated into Feline Kidney cells and observed for cythopathic effect (CPE). The presence of CPE indicated that there was viral isolate and continued to further identification using Haemaglutination (HA) test with pig red blood cells. Samples with positif in HA test were further identification using Haemaglutination Inhibition (HI) test against reference CPV antisera. Isolation result showed that from 81 samples processed, 10 samples indicated CPE in cell cultures, and had agglutinated in pig red blood cells and neutralised reference CPV antisera as the same titer of reference CPV antisera. Nine isolates were obtained from feces and 1 isolate was obtained from Mucose intestine from bloody diarrhoea dogs. Those isolates were also obtained from 1 to 2 days post blood diarrhea clinical signs. Two from 10 cadavers examined showed histological changes to CPV infection. Isolate of CPV, originally from feces, was also obtained from one of the two cadavers. Based on the results it was concluded that more CPV viral isolates can be obtained from bloody diarrhea feces.   Key words: Canine parvovirus, inhibition, isolation, pathology, identification
The development of an Enzyme Linked Immunosorbent Assay for detecting Injectious laryngotrachitis viral antibodies in chicken serum Indriani, Risa; Adjid, R.M Abdul; ., Darminto; Hamid, Helmy
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 2 (2002)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v7i2.285

Abstract

The aim of this study was to develop an Enzyme-linked immunosorbent assay (ELISA) for detection of antibody against gallid herpes virus, the causal agent of infectious laryngotracheitis (ILT) in chicken. Its application in experimental chicken under laboratory condition was also evaluated. Results showed that ELISA for ILT was developed successfully with sensitivity and specificity was 98% and 97,14% respectively. The ELISA was able to determine the dynamic of antibodies respond in experimental chickens following vaccination and artificial infection with ILT virus. It was concluded that this ELISA offers a simple, sensitive and specific antibody assay for detection of antibodies against ILT virus in chickens arising from vaccination or infection.   Key words: ELISA, antibody, chicken, Infectious laryngotrachitis
Pathogenicity and immunogenicity local isolat infectious laryngo tracheitis virus Indriani, Risa; Hamid, Helmy; Adjid, R.M Abdul; Saepulloh, Muharam
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 2 (2004): JUNE 2004
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v9i2.418

Abstract

Infectious laryngotracheitis (ILT) is an acute and contagious respiratory diseases of chicken. The virus is Gallid herpes and belong to family herpesviridae. Two local strains of ILT virus those were BGR-6 and BKS-3 were isolated and their pathogenicity and immunogenicity were further observed after five time pareses on coris allantoic of specific pathogenic free embryonated eggs. The pathogenicity of both isolates to be possible for use as seed vaccine were detected based on pathogenicity indices and antibody response. Experimental specific pathogenic free chicken in isolator cages were infected by the isolates using103EID50. ILT virus per dose. Clinical syndromes, pathological anatomic lesions, and immunological response were observed in the infected chickens and another group at uninfected chickens as a control. Results showed that either BGR-6 or BKS-3 caused clinical signs with ITPI scores of 0,05 and 0,03 respectively and there were no mortality of infected chickens. The top antibody responces of BGR-6 and BKS-3 were observed at OD 0.90 and 0.44 respectively. It can be concluded that BGR-6 and BBS-3 had low ITPI scores, but BGR-6 gave higher antibody response and can be used as a candidate for seed vaccine.   Key words: Infectious laryngotracheitis, ILT, BGR-6, BKS-3, pathogenicity, immunogenicity
The isolation of canine parvovirus and pathological changes of infected dogs Sendow, Indrawati; Hamid, Helmy
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 1 (2004): MARCH 2004
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v9i1.427

Abstract

The aims of this study are to isolate canine parvovirus (CPV) from the field case and to evaluate its histological aspects in CPV infected dogs. Samples were collected from intestine, intestine contained and mucose, as well as dogs feses from diarrhoea and blood diarrhoea. The suspension of those specimen was inoculated into Feline Kidney cells and observed for cythopathic effect (CPE). The presence of CPE indicated that there was viral isolate and continued to further identification using Haemaglutination (HA) test with pig red blood cells. Samples with positif in HA test were further identification using Haemaglutination Inhibition (HI) test against reference CPV antisera. Isolation result showed that from 81 samples processed, 10 samples indicated CPE in cell cultures, and had agglutinated in pig red blood cells and neutralised reference CPV antisera as the same titer of reference CPV antisera. Nine isolates were obtained from feces and 1 isolate was obtained from Mucose intestine from bloody diarrhoea dogs. Those isolates were also obtained from 1 to 2 days post blood diarrhea clinical signs. Two from 10 cadavers examined showed histological changes to CPV infection. Isolate of CPV, originally from feces, was also obtained from one of the two cadavers. Based on the results it was concluded that more CPV viral isolates can be obtained from bloody diarrhea feces.   Key words: Canine parvovirus, inhibition, isolation, pathology, identification
The development of an Enzyme Linked Immunosorbent Assay for detecting Injectious laryngotrachitis viral antibodies in chicken serum Indriani, Risa; Adjid, R.M Abdul; ., Darminto; Hamid, Helmy
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 2 (2002): JUNE 2002
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v7i2.285

Abstract

The aim of this study was to develop an Enzyme-linked immunosorbent assay (ELISA) for detection of antibody against gallid herpes virus, the causal agent of infectious laryngotracheitis (ILT) in chicken. Its application in experimental chicken under laboratory condition was also evaluated. Results showed that ELISA for ILT was developed successfully with sensitivity and specificity was 98% and 97,14% respectively. The ELISA was able to determine the dynamic of antibodies respond in experimental chickens following vaccination and artificial infection with ILT virus. It was concluded that this ELISA offers a simple, sensitive and specific antibody assay for detection of antibodies against ILT virus in chickens arising from vaccination or infection.   Key words: ELISA, antibody, chicken, Infectious laryngotrachitis
The development of an Enzyme Linked Immunosorbent Assay for detecting Injectious laryngotrachitis viral antibodies in chicken serum Indriani, Risa; Adjid, R.M Abdul; ., Darminto; Hamid, Helmy
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 2 (2002): JUNE 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.993 KB) | DOI: 10.14334/jitv.v7i2.285

Abstract

The aim of this study was to develop an Enzyme-linked immunosorbent assay (ELISA) for detection of antibody against gallid herpes virus, the causal agent of infectious laryngotracheitis (ILT) in chicken. Its application in experimental chicken under laboratory condition was also evaluated. Results showed that ELISA for ILT was developed successfully with sensitivity and specificity was 98% and 97,14% respectively. The ELISA was able to determine the dynamic of antibodies respond in experimental chickens following vaccination and artificial infection with ILT virus. It was concluded that this ELISA offers a simple, sensitive and specific antibody assay for detection of antibodies against ILT virus in chickens arising from vaccination or infection.   Key words: ELISA, antibody, chicken, Infectious laryngotrachitis