Articles

Insidensi Nematoda Gastrointestinal dan Protozoa pada Monyet Ekor Panjang (Macaca fascicularis) Liar di Taman Wisata Alam (TWA) Pulau Weh Sabang Rahmi, Erdiansyah; Hanafiah, Muhammad; Sutriana, Amalia; Hambal, Muhammad; Wajidi, Farid
Jurnal Ilmu-Ilmu Peternakan JIIP Volume XIII No. 6 Mei 2010
Publisher : Jurnal Ilmu-Ilmu Peternakan

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The research aims to find out the  incidence of gastrointestinal parasite in wild long tail macaque in wildlife  reserve  park, Weh  Island, Sabang. For  that purpose,  fecal  specimens  from 25 wild  long  tail macaques  in Weh  Island-  Sabang were  collected.  The  fresh  faeces  samples were  then  preserved  using AFA  (alcohol-formal-acetic)  liquid  prior  to  be  examined  in  Parasitology  Laboratorium,  Faculty  of Veterinary Medicine, Syiah Kuala University.   The  presence of gastrointestinal  parasite was  observed using centrifuge method as well as Boray  sedimentation method. The obtained data were tabulated and analyzed  descriptively.  The  result  revealed  that  two  parasite  species  were  found  in  gastrointestine; Eimeria  spp.    (12%)  and  Strongloides  spp.  (8%).  It  could  be  concluded  that  the  prevalence  of gastrointestinal  parasite  in wild  long  tail macaque  in wildlife  reserve  park, Weh  Island-  Sabang was considered mild.
Identification of Parasites in Snakehead Fish (Channa striata) in Meunasah Manyang Village Lhoknga Subdistrict Aceh Besar Umara, Adil; Bakri, Muttaqien; Hambal, Muhammad
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (50.491 KB) | DOI: 10.21157/j.med.vet..v8i2.3324

Abstract

The study aimed at identifying the types of parasites that infect snakehead (C. striata) fish as well as to determine the prevalence of the parasite species. A total of 51 snakehead fish was captured using nets and traps then examined at Parasitology Laboratory of Veterinary Medicine Faculty Syiah Kuala University Banda Aceh. Ectoparasites inspection was done by observation of the outer body organs as well as by making the mucus smear examined under a microscope, while endoparasites inspection was done by observation on the surface of the digestive tract and heart, and also thin and thick boold smear. The study revealed that two types of parasites that infect snakehead fish in irrigation Cot Balee were Pallisentis nagpurensis and Trichodina spp. It also found larvae obtained from the intestinal villi scrapings that have not been identified. A total of 96% snakehead fish infected by P. nagpurensis, 6% infested with Trichodina spp., and 10% infested by unidentified larvae.Key words: parasite, snakehead fish (Channa striata), Palllisentis nagpurensis, Trichodina spp, prevalence
Purifikasi Imunoglobulin Yolk Pada Ayam yang Divaksin terhadap Ekskretori/Sekretori Stadium L3 Ascaridia galli Darmawi, Darmawi; Balqis, Ummu; Tiuria, Risa; Hambal, Muhammad; Samadi, Samadi
Jurnal Agripet Vol 10, No 2 (2010): Volume 10, No. 2, Oktober 2010
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.859 KB) | DOI: 10.17969/agripet.v10i2.638

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Purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli L3 larvae stageABSTRACT. The main immunoglobulin fraction of poultry is called IgY, in order to distinguish it from the mammalian IgG. This article focus on purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli larvae to obtained purity IgY. Active vaccinations with excretory/secretory antigen were applied intra muscularly of chickens with an initial dose of 80 μg. The vaccinations were repeated three times with dose of each 60 μg with an interval of one week. The first vaccinations were excretory/secretory antigen mixed with Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete. Antibody response in yolk was detected at weekly intervals by agar gel precipitation test (AGPT). The chicken’s eggs were collected from 49 day after vaccinations. IgY was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ekscretory/secretory IgY protein was determined by Bradford method (λ = 280 nm). The result showed that antibody in yolk was begun detect with AGPT at four weeks after vaccination. IgY concentration after purification was 0,875 ± 0.251 mg/ml. This study has shown that the product released in vitro by L3 stage A. galli is capable of stimulating humoral immunity by mean of producing antibody through yolk as biologic manufacturer could be a good choice.
Perubahan Patologi Anatomi Hati dan Saluran Empedu Sapi Aceh Yang Terinfeksi Fasciola gigantica Balqis, Ummu; Darmawi, Darmawi; Aisyah, Siti; Hambal, Muhammad
Jurnal Agripet Vol 13, No 1 (2013): Volume 13, No. 1, April 2013
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.434 KB) | DOI: 10.17969/agripet.v13i1.554

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Pathology anatomy changes in liver and bile duct of Aceh cattle infected with fasciola giganticABSTRACT. This study was conducted in Banda Aceh Municipality, Aceh Province to report the pathological changes in liver of Aceh cattle infected with Fasciola gigantica. In this study, livers of male aceh cattles were collected from slaughter area when Qurban in Idul Adha during two years (2011 to 2012). A total of 15 aceh cattle livers were examined. Establishment of F. gigantica were observed, and pathological changes of liver were identified. The result showed that out of 15 male Aceh cattle livers 6 (40%) were found infected with F. gigantica. The common pathological changes found in this study were the hepatic damage, and the bile ducts were changes in size and shape with the dilated lumen. The bile ducts were found filled with blackish brown exudate, which contained adult F. gigantica. In conclusion, fascioliasis is greatly responsible for hepatic damage, and currently expanding as an important veterinary public health problem.
Kajian Titer Antibodi Pada Yolk dari Ayam yang Diimunisasi Dengan Antigen Ekskretori/Sekretori Stadium L3 Ascaridia galli Darmawi, Darmawi; Balqis, Ummu; Tiuria, Risa; Hambal, Muhammad; Samadi, Samadi
Jurnal Agripet Vol 8, No 2 (2008): Volume 8, No. 2, Oktober 2008
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (47.948 KB) | DOI: 10.17969/agripet.v8i2.611

Abstract

Evaluation of antibody titre in yolk from immunized chickens with excretory/secretory antigen of L3 stage of Ascaridia galliABSTRACT. The purpose of the present study was to trigger humoral immunity of chickens egg yolk exposed to excretory/secretory released in vitro by L3 stage of Ascaridia galli. Amount of 6 head chickens were divided into two groups. First group, the chickens were not immunized. Second group, the chickens were immunized with excretory/ secretory. Active immunizations with excretory/ secretory antigen were applied intra muscularly of chickens with an initial dose of 80 μg. The immunizations were repeated three times with dose of each 60 μg with an interval of one week. The first immunizations were excretory/secretory antigen mixed with Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete. Antibody response in yolk was detected at weekly intervals by enzyme linked immunosorbant assay (ELISA). The result showed that antibody in yolk was begun detect with ELISA increased at two weeks after immunization, This study has shown that the excretory/secretory released in vitro by L3 stage A. galli is capable of stimulating humoral immunity by mean of producing IgY in yolk.
Histopathological Changes of Chicken (Gallus domesticus) Small Intestine Naturally Infected by Ascaridia galli Balqis, Ummu; Hambal, Muhammad; Utami, Cut Syeila
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.037 KB) | DOI: 10.21157/j.med.vet..v8i2.3352

Abstract

This study was aimed at finding out the histopathological changes of chicken small intestines that were naturally infected by Ascaridia galli. Ten intestines were obtained from wet market in Banda Aceh then measured in length and divided into three sections: duodenum, jejunum and ileum, of which histologic slides were made. The number of Ascaridia galli founded in the lumen and ingesta were counted. Parameter picture of histopathologycal changes were scored. Results were analyzed descriptively. Duodenum, jejunum and ileum infected by Ascaridia galli showed hyperemia, hemorrhage, desquamation, inflamatory cell infiltration, histopathological changes of villi represented by desquamation, hyperplasia and fusion. Damage level of the small intestine chicken was largely determined by the number of infecting Ascaridia galli. It was concluded that the more number of Ascaridia galli that infect chickens, the more damage occurred in the small intestine of chicken.Key words: intestine, chicken, Ascaridia galli
Mucosal Mast Cells Contribution in Intestinal Defense of Chickens (Gallus domesticus) Infected Naturally by Ascaridia galli Balqis, Ummu; Admi, Masda; Darmawi, Darmawi; Hambal, Muhammad
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Abstract

This study was aimed at finding out the investigation of mucosal mast cells   in intestines of chicken  that  were  naturally  infected  by  Ascaridia galli.  Amount  of  ten  intestine  of  freshly slaughtered chickens (Gallus domesticus) found from local abatoir in Banda Aceh were divided into two groups containing five intestines of each. Mucosal mast cells count were done of which histologic slides were made in stained serial histological sections with Alcian blue (pH 0,3) and Safranin-O (pH 0,1) of the intestines. The result showed that the mucosal mast cells increased significantly (P < 0.05) in intestines of chickens infected naturally by survival A. galli adult worms. It was concluded that the intestinal defense of chickens against parasite infection is associated with the mucosal mast cells contribution by creating an environment hostile to the establishment and survival of intestinal nematodes, A. galli.
Enzyme activity of Ascaridia galli Llarvae was inhibited by Phenil Methanyl Methane Sulfonyl Fluoride Balqis, Ummu; Darmawi, .; Hambal, Muhammad
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Enzyme catalyse a broad spectrum of important biological reactions, including protein metabolism and immune reactions. A study was carried out to partial characterize enzyme from exretory/secretory of A. galli L3 stage based on influence of inhibitor. A. galli L3 were recovered from intestines of 100 heads chickens 7 days after oesophagus inoculation with 6000 L2. L3 recovered in this manner were cultured (5 – 10 ml-1) in flasks containing rosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Cultures were incubated at 370C in 5% CO2 and culture fluid was collected after 3 days in culture. Excretory/secretory was prepared from metabolic product of L3 released in culture medium. The enzyme activity was assayed against casein 2%. The amount of concentration was determined from the absorbance at 595 nm. Inhibitor sensitivity on enzyme activity was studied in phenil methanyl methane sulfonyl fluoride (PMSF) 0,5 and 1 mM.    The result showed that enzyme activity of A. galli larvae was inhibited by PMSF 0.5 and 1 mM, which enzyme activity remainded 1,8 and 0%, respectively. The results indicate that the excretory/secretory secreted by L3  A. galli contained serine protease
Poultry sellers’ perception and their effort for prevention strategy on avian influenza in Banda Aceh, Indonesia Darmawi, .; Ferasyi, Teuku R.; Muthmainnah, .; Balqis, Ummu; Fakhrurrazi, .; Darniati, .; Hambal, Muhammad
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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A study was conducted to examine some poultry sellers’ perception of the Avian influenza (birdflu) in nine locations (Ulee Kareng, Batoh, Lamnyong, Kampong Baru, Peunayong, Setui, Kampong Ateuk, and Neusu) in Banda Aceh Municipality, Indonesia. Primary data were obtained with the use of a structured questionnaire administared to ninety nine (99) respondents selected using proportional stratified random sampling, between November to December 2011. Information was obtained using the questionnaire sheets contain questions: knowladge, attitude, availability of facilities, and the effort of Avian influenza prevention strategy. Data obtained were analyzed by descriptive statistics. The result showed that only 52% of the poultry sellers’ had good knowledge of early symptomps of Avian influenza. Amount 59% of the poultry sellers’ had good attitudes. Result also showed that 73% of respondents had good facilities that was available in live poultry market area, but only 70% of respondents were good awareness about the effort of Avian influenza prevention strategy. In addition, the respondents who had more knowledge, good attitudes, and/or good facilities were also those who actually acted more preventively. Based on the result we concluded that the poultry sellers’ perception on prevention strategy for infection of birdflu in Banda Aceh categorized as poor. It is suggested that compliance with preventive measures may be enhanced through behavioural modification and building awareness through communication, training, workshop programs to the live poultry market sellers’ in Banda Aceh
Ovitrap use in epidemiology study of Aedes aegypti and Aedes albopictus in Kuta Alam sub-district Banda Aceh, Indonesia Farida, Athaillah; Ahmad, Abu Hasan; Hadi, Upik K.; Hambal, Muhammad; Fahrimal, Yudha; Shafitri, Rimaya
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

A study on the mosquitoes Aedes spp. has been conducted in Kuta Alam Sub-district, Banda Aceh, to find out the percentage and distribution of positive ovitrap with eggs of Aedes aegypti and A. albopictus, based on location of ovitrap, level of rainfall as well as different landuse of settlement areas. Sampling was conducted by setting up ovitrap monthly, from January to December 2010 in settlement area that have more trees versus less trees, both indoor and outdoor. Data of the ovitrap percentage that was proved to be positive with eggs of A. aegypti and A. albopictus was analized using Student T-Test. The study revealed that rainfall value did not significantly affect (P&gt;0.05) the percentage of positive A. aegypti and  A. albopictus ovitraps and neither  did the location of ovitrap (indoor versus outdoor). The settlement areas with more trees have significantly higher percentage of ovitraps containing A. albopictus than areas with less trees, but not significant for A. aegypti (P&lt;0.05). It was suggested that rainfall and location of ovitrap did not influence the number of ovitrap containing Aedes aegypti and Aedes albopictus eggs, while settlement landuse  influenced significantly the percentage of positive ovitraps for Aedes albopictus, but not for Aedes. aegypti. Between two villages in Kuta Alam sub-district, it was found out that Gampong Beurawe was dominated by Aedes albopictus while Gampong Kuta Alam by Aedes aegypti. Overall, the sensitivity of ovitrap in Kuta Alam subdistrict was 83,7%. The usage of ovitrap is thus recommended for monitoring of Aedes spp. control programs
Co-Authors . Darmawi . Darniati . Fakhrurrazi . Muthmainnah Abdul Harris Abdullah Hamzah, Abdullah Abu Hasan Ahmad Adil Umara, Adil Admi, Masda Agus Dermawan, Agus Agusty, Atika Ahmad, Abu Hassan Akbar, Muhammad Ridhan Al Azhar Amalia Sutriana Amiruddin A Andi Novita Anwar A, Anwar Aprilia Wardana, Aprilia Arman Sayuti Athailah, Farida Athaillah Farida Athaillah, Farida Athaillah, Farida Aulanni’am a Ayuni, Rizka Azhar Azhar Azhari A Azhari Azhari Bakrie, Muttaqien Basuki B. Purnomo Cut Dahlia Iskandar Cut S. Utami Cut Syeila Utami, Cut Syeila Darmawi D Darmawi Darmawi Dasrul Dasrul Dwina Aliza Dwinna Aliza Eliawardani Eliawardani, Eliawardani Ellyawardani, Ellyawardani Elsa Mariane Ramadani, Elsa Mariane Erdiansyah Rahmi Erina Erina Fachriyan Hasymi Pasaribu Farid Wajidi Farida Farida Febiola Rama Sari, Febiola Rama Ferasyi, T Reza fisdiora, zena Fitra Aji Pamungkas, Fitra Gholib G, Gholib Hamdani Budiman Hamny H, Hamny Hamny Hamny harliyanda, syafriza Hasan, Denny Irmawati Henni Vanda Ismail Ismail Jamil, Ikwan Joharsyah J, Joharsyah Khaira, Arini Ulfa M. Aris Widodo M. Nur Salim Maldini, Audi Maryam Maryam Muhammad Hanafiah Mukhtar, Rahmi Sulastri Mulya Fahmi, Mulya Mulyadi Adam, Mulyadi Mulyadi Mulyadi Murhaban Murhaban Muslim Akmal Muslina Muslina, Muslina Mustafa Sabri Muttaqien Bakri, Muttaqien Muttaqien Muttaqien, Muttaqien N.A., Zuhrawaty NA, Zuhrawaty Nurul Hidayati Nuzul Asmilia Parwis, Muhammad Rahmah, Taufikah Rahman, Resty Fauzana Rastina, Rastina Rastina, Rastina Razali Daud Razali R Razali Razali Retno Damayanti Soejoedoeno Rimaya Shafitri Rinidar R, Rinidar Rinidar Rinidar, Rinidar Risa Tiuria Rizki, Adistya Putra Roslizawaty r Rosmaidar Rosmaidar, Rosmaidar Ruskhanidar R, Ruskhanidar Rusli Rusli Saddat Nasution, Saddat Samadi Samadi Septian, Anugrah Siti Aisyah Sri Wahyuni Sugito s Sugito Sugito Suriadi S, Suriadi Sutiman B. Sumitro Syafruddin s T. Armansyah T. Fadrial Karmil T. Reza Ferasyi, T. Reza Tapielaniari -, Tapielaniari Teuku R. Ferasyi Teuku Reza Ferasyi Teuku Reza Ferasyi, Teuku Tongku Nizwan Siregar Triva Murtina Lubis Ummu Balqis Upik K. Hadi Wardani, Elia Winaruddin Winaruddin Wulan Windasari, Wulan Yudha Fahrimal Z, Zuhrawati Zainuddin Z Zainuddin Zainuddin Zakiah Heryawati Manaf Zarry, Muhammad Geraldy