Danny Halim
Grup Peneliti Sel Punca, Unit Penelitian Kesehatan, Fakultas Kedokteran Universitas Padjadjaran
Articles
5
Documents
Knowledge and attitude of pregnant woman toward umbilical-cord-blood collection

Indonesian Journal of Obstetrics and Gynecology Vol. 34. No 2. April 2010
Publisher : Indonesian Journal of Obstetrics and Gynecology

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Abstract

Objective: Stem cell technology from umbilical cord blood (UCB) has developed fast as health services. UCB could be used in many kinds of disease. Private UCB bank has been operated in many places all over Indonesia. There’s a need for research to evaluate the knowledge and attitude of pregnant woman towards the act of collecting UCB.Method: This is a cross-sectional study base on question-naire given to 163 pregnant women in three private hospitals in the Bandung City from September - November 2009. The result was analyzed with Rank-spearman correlation and Kruskal-Wallis chisquare.Result: Most respondent were 20 - 29 years old pregnant women (54.6%), with educational level of strata-1 (S-1) (41.1%), with monthly income within 5 - 10 million rupiah (35.6%). This is the second pregnancy or more (42.9%), with gestational age less than 24 weeks (61.3%) and variative obstetric history (59.5%). The average level of knowledge was poor and attitude scale was uncertain (3.4). There is a significant correlation between age (p = 0.008%; CI 95%), level of education (p = 0.0001; CI 95%) and knowledge. There is a significant correlation between gestational age and attitude (p = 0.003; CI 95%). Respondent’s level of knowledge also has a significant correlation with attitude (τs = 0.421; p = 0.0001; CI 95%).Conclusion: This study described the respondent’s poor knowledge will influence their attitudes toward UCB collection, so it is necessary for disseminating more information on UCB as an effort to get better research result on UCB.[Indones J Obstet Gynecol 2010; 34-2: 97-100]Keywords: knowledge level, attitudes, collecting UCB, pregnant woman, the Bandung city Tujuan: Tujuan: Teknologi sel punca yang berasal dari darah tali pusat (DTP) saat ini berkembang dengan pesat untuk pelayanan kesehatan. DTP ini dapat dipergunakan pada berbagai macam penyakit. Beberapa cabang bank DTP swasta telah beroperasi di Indonesia. Sehingga perlu dilakukan penelitian tingkat pengetahuan dan sikap ibu hamil terhadap pengumpulan DTP. Metode: Dilakukan penelitian survey cross-sectional pada 163 orang ibu hamil di tiga rumah sakit swasta Kotamadya Bandung sejak September - November 2009. Kuisioner berupa kuesioner tertutup.Hasil yang di dapat dianalisis dengan menggunakan uji korelasi Rank-Spearman dan uji chi-kuadrat Kruskal-Wallis. Hasil: Karakteristik terbesar responden berusia 20 - 29 tahun (54,6%), berpendidikan S-1 (41,1%), pendapatan perbulan 5 sampai 10 juta rupiah (35,6%). Kehamilan saat ini adalah kehamilan yang ke-2 atau lebih (42,9%), usia kehamilan kurang dari 24minggu (61,3%) dan riwayat obstetri yang bervariasi (59,5%). Rerata tingkat pengetahuan kurang (50,97%) dan skala sikap raguragu (3,4). Didapatkan hubungan bermakna antara usia (p = 0,008%; CI 95%) dan tingkat pendidikan terhadap tingkat pengetahuan (p = 0,0001; CI 95%). Terdapat hubungan bermakna antara usia gestasi dengan sikap responden (p = 0,003; CI 95%). Tingkat pengetahuan responden juga bermakna secara statistikterhadap sikap responden (τs = 0,42; p = 0,000; CI 95%).Kesimpulan: Penelitian ini menggambarkan tingkat pengetahuan responden yang rendah akan mempengaruhi sikap terhadap pengumpulan DTP, sehingga dibutuhkan penyebaran informasi yang lebih baik lagi mengingat manfaat yang didapat melalui penelitian DTP.[Maj Obstet Ginekol Indones 2010; 34-2: 97-100]Kata kunci: tingkat pengetahuan, sikap, pengumpulan darah tali pusat, ibu hamil, Kotamadya Bandung

Embryo Quality: The Most Critical Factor for Pregnancy Rates after day-2, day-3, and day-5 of Embryo Transfer

Indonesian Journal of Obstetrics and Gynecology Vol. 34. No. 4. October 2010
Publisher : Indonesian Journal of Obstetrics and Gynecology

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Abstract

Objective: To determine the most critical factor on day-2, day-3, and day-5 of embryo transfer in correlation with pregnancy rates.Method: This research is a retrospective study in Aster Fertility Clinic, IVF program- Dr. Hasan Sadikin Hospital, Bandung, Indonesia. One hundred ninety five women enrolled in an IVF program conducted from March 2006 through November 2009 at the Aster Fertility Clinic. Effect of embryo-transfer day and any other factors (including quality of embryo, oocyte quantity, difficulty of embryo transfer technique, and blood or mucus contamination on the catheter) on pregnancy rate in IVF.Results: The mean age of the pregnant group was 34.65 (SD = 3.91), and the mean of the infertility period was 7.25 years (SD = 3.54). There were no siginificant differences in pregnancy rates in the day-2, day-3, and day-5 groups. The most critical factor influencing pregnancy was the total score for the quality of embryos [p = 0.001; OR (CI 95%) = 1.94 (0.91 - 4.08)]. Otherwise, the day of embryo transfer, oocyte quantity, and difficulties in embryo transfer did not affect the pregnancy rate (p > 0.05).Conclusion: Our study suggests that the total score for the quality of the embryos was the most critical factor for the success rate of pregnancy rather than the day of embryo transfer, oocyte quantity, difficulty of embryo transfer technique, or contamination of blood and mucus on the catheter.[Indones J Obstet Gynecol 2010; 34-4: 175-9]Keywords: day of embryo transfer, in vitro fertilization, quality of embryo total score, oocytes quantity, catheter contaminationTujuan: Untuk menentukan faktor yang terpenting pada keberhasilan kehamilan setelah transfer embrio hari ke-2, ke-3 dan ke-5.Metode: Penelitian ini adalah studi retrospektif di klinik Fertilitas Aster, Program IVF- Rumah Sakit Dr. Hasan Sadikin, Bandung, Indonesia. Penelitian ini melibatkan seratus sembilan puluh lima perempuan yang mengikuti program IVF sejak Maret 2006 hingga Nopember 2009. Efek dari waktu (hari) transfer embrio dan faktorfaktor lainnya (termasuk kualitas embrio, jumlah oosit, kesulitan dalam teknik transfer embrio, dan kontaminasi darah atau mukus dalam kateter) terhadap angka keberhasilan kehamilan pada IVF.Hasil: Rata-rata usia perempuan hamil pada penelitian ini 34,65 (SD = 3,91), dan rata-rata periode infertilitas 7,25 (SD = 3,54). Tidak terdapat perbedaan bermakna pada angka kebehasilan kehamilan setelah transfer embrio baik pada hari ke-2, -3 dan -5. Faktor terpenting yang mempengaruhi keberhasilan kehamilan adalah skor total dari kualitas embrio [p = 0,001; OR (CI 95%) = 1,94 (0,91 - 4,08)]. Dilain fihak, waktu transfer embrio, jumlah oosit, kesulitan dalam teknik transfer embrio tidak mempengaruhi kebehasilan kehamilan (p > 0,05).Kesimpulan: Hasil studi kami menunjukkan bahwa faktor terpenting yang mempengaruhi keberhasilan kehamilan adalah skor total dari kualitas embrio dibandingkan dengan waktu transfer embrio, jumlah oosit, kesulitan dalam teknik transfer embrio, atau kontaminasi darah dan mukus dalam kateter.[Maj Obstet Ginekol Indones 2010; 34-4: 175-9]Kata kunci: hari setelah transfer embrio, fertilisasi in vitro, skor total dari kualitas embrio, jumlah oosit, kontaminasi kateter

Uji Fungsional dan Karakteristik Sel Punca Hematopoetik Hasil Isolasi dari Darah Tali Pusat Manusia Menggunakan Metode Modifikasi Unpad- Aster

Majalah Kedokteran Bandung Vol 43, No 4 (2011)
Publisher : Fakultas Kedokteran, Universitas Padjadjaran

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Abstract

Metode isolasi sel-sel mononuklear/mononuclear cells (MNCs) dari darah tali pusat (DTP) manusia secara konvensional menghasilkan tingkat kontaminasi sel eritrosit yang sangat tinggi. Oleh karena itu, tujuan penelitian ini adalah menilai perbedaan viabilitas dan kontaminasi sel eritrosit dalam populasi MNC DTP pada modifikasimetode isolasi yang kami kembangkan. Penelitian ini juga bertujuan untuk menguji fungsi dan karakteristik populasi MNCs dari DTP manusia sebagai dasar pembangunan bank darah tali pusat di Indonesia. Penelitian dilakukan di Departemen Obstetri dan Ginekologi RS Dr. Hasan Sadikin Bandung periode Januari–Oktober 2010. Isolasi MNCs dengan metode modifikasi (dinamakan modifikasi Unpad-Aster) yang menghasilkan 5,1x106 sel/mL memiliki tingkat kontaminasi sel eritrosit yang lebih rendah dibandingkan dengan metode konvensional. Morfologi sel yang dibiakkan dalam medium unrestricted somatic stem cells (USSCs) tampak seperti sel-sel yang adheren (menempel di dasar), berbentuk sel spindle, dengan cluster of differentiation-90 (CD-90) (antigen leukosit) dan cluster of differentiation-105 (CD-105) yang positif serta dapat berdiferensiasi menjadi sel neuron dan adiposit; sedangkan morfologi untuk cord blood-derived multipotent progenitor cells (CB-MPCs) tampak seperti sel-sel fibroblas dengan cluster of differentiation-45 (CD-45) (antigen hematopoetik) yang positif serta dapat berdiferensiasi menjadi sel neuron. Disimpulkan bahwa metode modifikasi Unpad-Aster memberikan tingkat kontaminasi eritrosit yang lebih rendah dibandingkan dengan metode konvensional. Sel mononuklear yang berasal dari darah tali pusat ini dapat berdiferensiasi menjadi sel-sel neuron dan adiposit. [MKB. 2011;43(4):171–7].Kata kunci: Darah tali pusat (DTP), diferensiasi, karakterisasi, modifikasi Unpad-Aster, sel mononuklearFunctional Test and Characteristic of Hematopoietic Stem Cells Derived from Human Umbilical Cord Blood Using Unpad-Aster’s Modified MethodThe conventional method of mononuclear cells (MNCs) isolation from human umbilical cord blood (UCB) yielded high erythrocyte contamination level. Therefore, the aim of this study was to assess the differences of cell viability and erythrocyte contamination on the population of UCB MNCs in our modified isolation method. This study was also aimed to test the function and characteristic of human MNCs derived from UCB as the basis for the development of UCB banking in Indonesia. The study was conducted in Department of Obstetry and Ginecology >RS Dr. Hasan Sadikin Bandung in period of January–October 2010. The modified isolation method (namely Unpad Aster’s modification) yielded 5.1x106 MNC cell/mL has lower erythrocyte contamination level than conventional method. The morphology of MNCs cultured in unrestricted somatic stem cells (USSCs) medium looked like adhered cells (attached at the surface of culture flask), spindle-shaped cells with positive luster of ifferentiation-90 (CD-90) (leukocyte antigen) and cluster of differentiation-105 (CD-105) and could differentiate into neuronal cells and adipocytes. While the morphology of cord blood-derived multipotent progenitor cells (CB-MPCs) looked like fibroblast cells with positive cluster of differentiation-45 (CD-45) (antigen hematopoietic) and could differentiate into neuronal cells. In conclusions, the Unpad-Aster’s modified isolation method gives lower level of erythrocyte contamination compared with conventional method. Mononuclear cells derived from UCB could differentiate into neuronal cells and adipocytes. [MKB. 2011;43(4):171–7].Key words: Characteristic, differentiation, mononuclear cells (MNCs), umbilical cord blood, Unpad-Aster modification

Cancer Stem Cell: Target Baru Obat Antikanker

Indonesian Journal of Cancer Vol 4, No 3 (2010): Jul - Sep 2010
Publisher : "Dharmais" Cancer Center Hospital

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Abstract

Relaps, resistance and metastasis has become prominent problems that oncologists and cancer patients have to dealt with. Various studies have been done previously concluded that there are a subpopulation of cancer cells, identified as cancer stem cell, most likely to be the cause of relaps, resistance and metastasis of cancer. Cancer stem cell is a subpopulation of cancer cells that possess tumorigenicity, hence it can initiate the growth of tumor. Cancer stem cell has been suspected to be originated from normal stem cells reside in mature tissues, or from progenitor cells that gone through some series of alterations on its characteristics, including mutagenic and non-mutagenic changes. As seen in normal stem cells, cancer stem cell is also oftenly found in its inactive state. Therefore, cancer stem cell is not affected when it treated with many chemotherapeutic agents that are targeting cancer cells that proliferate extensively. Eventually, this event leads to the incidence of cancer relaps on cancer patients who already had series of cancer therapy. Based on this knowledge, it can be concluded that the only absolute way to overcome the incidence of metastasis, resistance and relaps on cancer patients, is to targeting cancer stem cell. Therefore, optimization on protocols of cancer stem cell identification and isolation strived continously. Some molecular markers that are oftenly used as a standard on cancer stem cell isolation are CD34, CD44 and CD133. In line with that, isolation methods that are based on sphere formation and the absorption of coloring dye could also be done to obtain cancer stem cell population. This review article would like to explain the nature of cancer stem cell existence, the pathology underlies its formation, characteristics and identification techniques that are commonly used, and challenges that have to be faced by scientists and physicians in order to optimize the application of cancer stem cell theory for the progress of science and patients’ sake.

Modifikasi Metode Isolasi Sel Endotel Pembuluh Darah Otak (EPDO) Tikus: Teknik Dasar Kultur Sel Primer di Bidang Neurosains

Majalah Kedokteran Bandung Vol 42, No 4 (2010)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Metode konvensional isolasi sel endotel pembuluh darah otak (EPDO) masih tergolong sulit, sehingga upaya mendapatkan populasi murni sel ini adalah tantangan. Pada penelitian ini dilakukan isolasi endotel dari tikus Wistar dan mencit C57/Bl6, berdasarkan protokol the care and use of laboratory animals, Universitas Gunma, Jepang. Modifikasi metode isolasi adalah menggunakan gradasi bovine serum albumin (BSA), bukan Dextran-70 yang umumnya dipakai, untuk memisahkan sel EPDO yang bersatu menjadi sel EPDO tunggal. Penelitian ini dilakukan di laboratorium sel kultur, Universitas Padjadjaran bekerjasama dengan Universitas Gunma, Jepang, Januari 2008–Juni 2009. Uji hasil isolasi dan karakteristik sel EPDO dilakukan dengan teknik imunofloresen. Ekspresi tight junction ZO-1, menunjukkan sel EPDO membentuk selapis sel utuh, rapat, tidak bertumpuk dan kompak, sesuai dengan karakteristik dinding EPDO. Fenotip sel EPDO dikonfirmasi dengan acethylated LDL, faktor von Willebrand dan CD31. Penghancuran kapiler dengan collagenase/dispase masih menghasilkan populasi sel yang terkontaminasi perisit. Kontaminasi dimurnikan dengan menggunakan puromycin, tingkat pemurnian sel EPDO mencapai 98,3%. Simpulan, teknik modifikasi berhasil mengisolasi sel EPDO tikus dan mencit, tanpa melakukan intervensi genetik. Puromycin dapat digunakan untuk memurnikan sel EPDO. [MKB. 2010;42(4):161–8].Kata kunci: Metode modifikasi isolasi sel EPDO, pembuluh sawar otak, teknik pemurnian Isolation Modified-Method of Mouse-Brain Microvessel Endothelial Cells: Primary Cell Culture Technique in NeuroscienceIsolation method to obtain pure BMVECs is hard to be done consistently and remains a challenge. In this study, we isolated BMVECs from Wistar rat and C57/Bl6 mouse from Japan SLC. All procedures performed according to guidelines for the care and use of laboratory animals of Gunma University, Japan. The modification of isolation method was using bovine serum albumin (BSA) gradation, not Dextran-70 in which generally used, to separate clusters of BMVECs into single cell. This study was done at Universitas Padjadjaran, in colaboration with Gunma University, Japan, January 2008–June 2009. Further,characteristic and purification results were proven by imunofluorescene staining. The results showed that staining of tight junction, ZO-1, formed a monolayer, tightly packed, non-overlapping and contact-inhibited BMVECs, as expected for a vessel wall endothelial. ECs phenotype confirmed by acethylated LDL, von Willebrand and CD31. The digestion of capillaries generated contaminating pericytes. Contamination was purified using puromycin and the results considered satisfactory (98.3%). In conclusion, our modification procedure allows the isolation of primary rat and mouse BMVECs, which form an endothelial-like monolayer in few days. Puromycin can be used for purification of primary rat and mouse BMVECs. [MKB. 2010;42(4):161–8].Key words: Blood brain barrier, isolation modified-method of mouse-BMVECs, purification methods DOI: http://dx.doi.org/10.15395/mkb.v42n4.30