Achmad Fuad Hafid
Faculty of Pharmacy, Airlangga University, Surabaya

Published : 4 Documents
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Combination Therapy Model of Cempedak Stembark Ethanol 80% Extract (Artocarpus champeden Spreng.) and Artesunat on Malaria Parasite Infected Mice Hafid, Achmad Fuad; Tyas, Maharani Wahyuning; Widyawaruyanti, Aty
Journal of the Indonesian Medical Association Vol. 61 No. 4 April 2011
Publisher : Journal of the Indonesian Medical Association

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Abstract

Parasite resistance to antimalarial become a major problem in health. Combination therapy with artemisinine derivates or known with term ACT (Artemisinine-based Combination Therapy) was suggested by WHO as alternative therapy to control the spreading resistance of Plasmodium falciparum. Combination therapy of Cempedak stembark (Artocarpus champeden Spreng.) 80% Ethanolic Extract (ACEE) and Artesunate on Plasmodium berghei infected mice was studied. The study was carried out by oral administration of ACEE and artesunate. Treatment groups were divided in (A) ACEE 100 mg/kg bw daily for three concecutive days; (B) Artesunate 36,4 mg/kg bw daily for three consecutive days; (C) combination of ACEE 100 mg/kg and Artesunate 36,4 mg/kg daily for three concecutive days; (D) combination of ACEE 100 mg/kg daily for three concecutive days and Artesunate 36.4 mg/kg daily at day one; (E) combination of ACEE 100 mg/kg for three concecutive days and Artesunate 36.4 mg/kg daily at day three. The result showed that A, B, C, D, and E inhibit parasite’s growth for 62.6%; 66.8%; 82.3%; 67.8%; 70.2% respectively. It showed that C gives the highest antimalarial activity among all groups. Based on one way Anova, the treatment groups showed statistically significant difference (p=0.00; p<0.05) for inhibiton of parasite growth. The research revealed that combination of ACEE 100 mg/kg and Artesunate 36.4 mg/kg daily for three concecutive days (model C) on P.berghei infected mice is the most potential therapy model.Keywords: Artocarpus champeden extract, combination therapy, Artesunate,inhibition of parasite’s growth.
Skrining Aktivitas Antimalaria Beberapa Tanaman Indonesia Hasil Eksplorasi Dari Hutan Raya Cangar, Batu-Malang, Jawa Timur Hafid, Achmad Fuad; Puliansari, Nike; Lestari, Nur Suci; Tumewu, Lidya; Rahman, Abdul; Widyawaruyanti, Aty
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol 3, No 1 (2016): Jurnal Farmasi dan Ilmu Kefarmasian Indonesia
Publisher : Fakultas Farmasi Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.387 KB) | DOI: 10.20473/jfiki.v3i12016.7-11

Abstract

Background: Malaria is the most important parasitic disease. Malaria control which depends on specific chemotherapy now complicated by resistance of Plasmodium falciparum to most commonly available antimalarial drug. Such situation has heralded the need for alternative antimalarial therapy. Objective: This research aim was to find new antimalarial candidates from some Indonesia plants collected from Cangar National Forest, Batu-Malang, East Java. Methods: Eleven samples of leaves and stem extracts were screened against Plasmodium falciparum 3D7 culture which maintained in RPMI-1640 Medium. Samples tested in concentration of 0.01, 0.1, 1, 10 and 100 µg/ml. Probit analysis was used to determine IC50. Results: In vitro antimalarial activity revealed that only three crude extracts samples from Fraxinus griffithi stem extract, Piper sulcatum leaves extract and Eucalyptus globulus stem extract had good antimalarial activity with IC50 value of 0.33, 0.20 and 0.55 µg/ml, respectively. Conclusions: Fraxinus griffithi stem extract, Piper sulcatum leaves extract and Eucalyptus globulus stem extract might be a good candidate for antimalarial natural product resources.
Aktivitas Antimalaria Ekstrak Kulit Batang Cempaka Kuning Terhadap Plasmodium Falciparum 3D7 ARIANTARI, NI PUTU; RUSTINI, NI LUH; TUMEWU, LIDYA; HAFID, ACHMAD FUAD; WIDYAWARUYANTI, ATY
JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 1 (2013): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Cempaka kuning (Michelia champaca) merupakan tanaman dari suku Magnoliaceae, yang secara tradisional telah digunakan untuk pengobatan demam. Penelitian ini dilakukan untuk mengevaluasi aktivitas antimalaria ekstrak kulit batang cempaka kuning secara in vitro terhadap Plasmodium alcipamm 3D7. Kulit batang cempaka kuning diekstraksi dengan n-heksana, kloroform dan metanol sehingga diperoleh ekstrak n-heksana, ekstrak kloroform dan ekstrak metanol. Ketiga ekstrak tersebut selanjutnya diuji aktivitas antimalarianya. Skrining titokirnia terhadap ketiga ekstrak tersebut menunjukkan bahwa ekstrak n-heksana mengandung minyak atsiri dan terpenoid, ekstrak kloroform mengandung minyak atsiri, terpenoid dan llavonoid, serta ekstrak metanol mengandung minyak atsiri, terpenoid, flavonoid, tanin dan glikosida. Hasil penelitian ini juga menunjukkan bahwa ketiga ekstrak yang diuji aktif secara in vitro terhadap P. falciparum 3D7, dengan nilai IC50 masing-masing sebesar 0,36 µg/mL untuk ekstrak n-heksana; 0,24 µg/mL untuk ekstrak kloroform dan 1,00 µg/ mL untuk ekstrak metanol. Ekstrak kulit batang Cempaka kuning memiliki aktivitas antimalaria sangat baik dan potensial untuk dikembangkan sebagai antimalaria.
Profil Fitokimia Ekstrak Etanol Bunga Kasumba Turate (Carthamus tinctorius L.) yang Berpotensi Sebagai Antimalaria Hamsidi, Rini; Widyawaruyanti, Aty; Hafid, Achmad Fuad; Ekasari, Wiwied; Malaka, Muhammad Hajrul; Kasmawati, Henny; Akib, Nur Illiyyin; Wahyuni, W; Sabarudin, S
Pharmauho Vol 4, No 2 (2018): Pharmauho
Publisher : Universitas Halu Oleo

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Bunga Kasumba turate (Carthamus tinctorius Linn) dari suku Asteraceae merupakan tumbuhan obat tradisional etnis Sulawesi Selatan yang secara empiris digunakan dalam pengobatan campak. Ekstrak etanol dari kasumba turate memberikan peningkatan aktivitas imunoglobulin G (IgG) dan  aktivitas imunoglobulin A (IgA) yang sangat signifikan. Penelitian menunjukkan ekstrak etanol bunga C. Tinctorius L memiliki potensi sebagai antimalaria secara in vitro dengan nilai IC50 sebesar 1,06 µg/ml. Sampel bunga C. tinctorius L. kering sebanyak 3 Kg dicacah hingga menjadi serbuk sebanyak 870 gram kemudian diekstraksi secara maserasi dengan menggunakan pelarut etanol 80%. Hasil maserasi dipekatkan dengan vacuum rotary evaporator pada suhu 50°C sehingga diperoleh ekstrak kental sebanyak 178,7gram. Profil fitokimia menunjukkan ekstrak etanol bunga C. tinctorius L. mengandung senyawa metabolit antara lain saponin, terpenoid, flavonoid, tanin dan antrakuinon.Kata kunci: Skrining, ekstrak, kasumba turate, obat tradisional, antimalaria