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Functional Response of the Parasitoid Eriborus argenteopilosus (Cameron) to Crocidolomia pavonana (Fabricius) under Different Temperature

HAYATI Journal of Biosciences Vol 12, No 1 (2005): March 2005
Publisher : Bogor Agricultural University, Indonesia

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Abstract

The functional response of the parasitoid Eriborus argenteopilosus (Cameron) (Hymenoptera: Ichneumonidae) were studied using Crocidolomia pavonana (Fabricius) (Lepidoptera: Pyralidae) larvae as host at different densities. All larvae were exposed to one E. argenteopilosus female for three hours at three different temperatures, i.e 20 oC, 25 oC, and 30 oC. Data were analyzed using logistic regression to determine the type of functional response. At 20 oC E. argenteopilosus showed type II functional response, while at 25 oC and 30 oC the functional response is type III. Based on surface analysis, the optimal parasitism rate occurred at 22.24 oC with 10.12 larvae parasitized/hour. The larvae density to achieve optimal parasitism rate was 69.38 larvae. The optimal oviposition rate was 12.59 eggs/hour which occurred at 24.41 oC, using a density of 94.54 larvae.

Inokulasi Fungi Mikoriza Arbuskula (FMA) Indigenus pada Bibit Jahe untuk Pengendalian Penyakit Layu Ralstonia solanacearum ras 4)

Jurnal Natur Indonesia Vol 14, No 01 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

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Abstract

Bacterial wilt disease caused by Ralstonia solanacearum race 4 is one of factors inhibiting ginger production. The study on ArbuscularMychorrizae Fungus (AMF) in greenhouse was found could reduce of bacteria wilt disease. Experiment design of research was randomizedblock design using 8 isolates. The results showed that inoculation of Arbuscular Mychorrhizae Fungus isolated from healthy gingerrhizosphere as biological diseases control agents could reduces disease severity. Four isolate could reduced disease severity up to 100% andsupport plant growth and production. As the result the seedling formation increased by 50–150%, plant height 36.92–87.56%, the numberof leaf 61.94–162.22% and ginger yield 190.62–400% respectively, compared to the control.

Penapisan Bakteri Endofit Akar Kedelai Secara in Planta untuk Mengendalikan Penyakit Pustul Bakteri

Jurnal Fitopatologi Indonesia Vol 8, No 4 (2012)
Publisher : Jurnal Fitopatologi Indonesia

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Abstract

Endophytic bacteria reside within plant hosts without causing disease symptoms. Bacterial endophytes promote plant growth and yield, suppress pathogens, may help to remove contaminants, solubilize phosphate, or contribute assimilable nitrogen to plants. The aim of this study were to obtain indigenous endophytic bacterial isolates, which have the ability to control bacterial pustule cause by Xanthomonas axonopodis pv. glycines and to increase growth and yield of soybean. The bacterial endophyte isolates were introduced as seed treatment (108 cfu/ml) and soil drench on 3 weeks old soybean seedling. Xanthomonas axonopodis pv. glycines were inoculated on one month old of soybean by pricking leaf methodes. The effect of bacterial endophyte on disease incidence, disease severity, plant growth and yield of soybean were evaluated. We have found two selected bacterial endophyte isolates from soybean’s rhizosphere (St4E1.1 and St1E1.1.), with the ability to control bacterial pustule and to increase growth and yield of soybean.Key words: bacterial endophyte, bacterial pustule, in planta technique, soybean

Detection and Sequence Diversity of Begomovirus Associated with Yellow Leaf Curl Disease of Pepper (Capsicum annuum) in West Sumatra, Indonesia

Microbiology Indonesia Vol 3, No 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

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Abstract

Yellow leaf curl disease of pepper has become an emerging important disease in West Sumatra since early 2000. Several attempts have been made, including disease survey and detection, in order to identify the causal agent of the disease. Pepper (Capsicum annuum) plants showing yellow leaf curl from West Sumatra were analyzed for presence of Begomovirus employing Polymerase Chain Reaction (PCR) with degenerate primers pAL1v 1978 and pARc 715. A DNA fragment of 1.6 kb was successfully amplified and subjected to direct sequencing. A stem loop region was found in the nucleotide sequence obtained, which contains the conserved nucleotide signature sequence TAATATTAC present in begomoviruses. Based on the stem loop region comparisons and phylogenetic analysis, the virus isolates from West Sumatra showed the closest relationship to Pepper yellow leaf curl Indonesia virus (PYLVIV) and Tomato yellow leaf curl Indonesia virus (TYLCIV). The sequence was different from other Asia Begomoviruses reported earlier. These isolates were divided into three groups which were tentatively called Pepper yellow leaf curl Indonesia virus-West Sumatra-[group 1], -[group 2] and -[group 3] {PYLCIV-WS-[group1], -[group2], and -[group3]}.

Formulation of Indigenous Rhizobacterial Isolates from Healthy Soybean’s Root, which Ability to Promote Growth and Yield of Soybean

International Journal on Advanced Science, Engineering and Information Technology Vol 4, No 5 (2014)
Publisher : International Journal on Advanced Science, Engineering and Information Technology

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Abstract

Plant growth promoting rhizobacteria are a group of bacteria, that actively colonize plant roots, induce the resistance of plant to pathogen, increase plant growth and yield. Our previous research had showed, that one of rhizobacterial isolates from soybean root effective to promote growth and yield of soybean. To maintain the effectivity of this bacterial isolate during storage, transportation and application, so need to be formulated. The aim of this research was to get the best carrier for formulation to maintain the effectivity of rhizobacterial isolate in storage to promote growth and yield of soybean. This research have used random complete design with 16 treatments and 3 replicates. The treatments were combination of material carrier for formulation of rhizobacterial isolate (peat soil, tapioca flour and coconut water + 1 % palm oil) and time of storage of formula (0, 1, 3, 5 and 7 weeks) and control. The parameter were population density of rhizobacterial isolate on formula, growth development (germination rate, plant height, number of leaves and twigs) generative phase (time of flowering and pod) and yield (weight of seed) of soybean. The results showed that all formulas of rhizobacterial isolate  able to increase growth and yield of soybean. The best combination which effective to increase growth and yield of soybean were rizobacteria in peat soil formula and storage for five weeks.

Karakteristik Morfologi dan Fisiologi Isolat Pantoea stewartii subsp. stewartii pada Jagung

Jurnal Fitopatologi Indonesia Vol 10, No 2 (2014)
Publisher : Jurnal Fitopatologi Indonesia

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Abstract

Pantoea stewartii subsp. stewartii is the pathogen causing stewart’s wilt disease in maize. The occurrence of the disease was recently reported in Indonesia with limited information. Research was conducted to characterize several isolates of P. stewartii subsp. stewartii isolated from maize plants collected from 6 districts in West Sumatra. Leaves showing yellow stripes symptoms along the vein was ground and suspeneded then the bacteria was isolated and incubated for 3 × 24 h at room temperature in nutrient glucose agar (NGA). Pure bacterial colonies gained from this medium was used for characterization. Assessment of bacterial characters was conducted based on morphological assay, physiological assay, biological assay (hypersensitivity, and pathogenicity) followed by polymerase chain reaction (PCR)-based identification. Unique characters of the family Enterobacteriaceae was observed on 7 isolates based on morphological and physiological characters, and only 4 isolates (PR1, PP, ST1, LA) gave positive reaction on hypersensitivity and pathogenicity test. Further molecular identification confirmed 3 out of 7 isolates had high homology to Pantoea stewartii, i.e. Pantoea stewartii strain R1-104, R1-132, and ATCC 29923 for isolates LA, PR1, and PR2, respectively.

Potensi serangga pengunjung bunga sebagai vektor penyakit darah bakteri (Ralstonia solanacearum Phylotipe IV) pada pisang di Sumatera Barat

Jurnal Entomologi Indonesia Vol 9, No 1 (2012): April
Publisher : Perhimpunan Entomologi Indonesia

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Abstract

Blood diseases caused by Ralstonia solancearum Phylotype IV is a major cause of production loss of banana in Indonesia, particularly for areas in West Sumatera. Currently there is a lack of information on blood diseases. The objective of this study was to obtain data of insect diversity and its potential as a dissemination agent of R. solanacearum Phylotype IV in West Sumatera. This research was conducted with a purposive sampling method in the Tabek Panjang highlands, District of Baso, Agam Regency and Pasar Usang lowland, district of Batang Anai, Pariaman Regency, West Sumatera. The results showed that banana plants infected by R. solanacearum Phylotype IV have a high diversity of flower-visiting insects. The diversity of insects in the lowlands is higher than that in highland and mainly are dominated by Trigona spp. (Hymenoptera: Apidae), Drosophila sp. (Diptera: Drosophilidae). To test the potential of both insect as vector of the blood diseases, isolated and identification of the bacteria using triphenyl tetrazolium medium chlorid (TTC) was used. Result showed that bacteria isolated from both insect are R. solanacearum hence providing evidence of the insect as vector of the blood diseases. Both insects have the potential to be vector of R. solanacearum Phylotype IV in West Sumatera. The identification of the bacteria that causes the disease. The identification of bacteria that is spread by flower visitors insects are R.solanacearum Phylotipe IV.

INDUKSI KETAHANAN TANAMAN JAHE TERHADAP PENYAKIT LAYU Ralstonia solanacearum RAS 4 MENGGUNAKAN FUNGI MIKORIZA ARBUSKULAR (FMA) INDIGENUS

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 11, No 1 (2011): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

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Abstract

Bacterial wilt disease caused by Ralstonia solanacearum race 4 is a yield-limiting disease of ginger. A study to investigate the ability of indigenous Arbuscular Mycorrhizae Fungi (AMF) to reduce bacteria wilt disease has been done. This research was designed by using Randomized Block Design (RBD) at greenhouse experiments, using eight AMF isolates with six replication. As supporting data, population R. solanacearum race 4 after treatments was assesed. The results showed that aplication of indigenous AMF  to ginger rhizome as seed treatment dicreased disease incidence, support ginger growth and production such as the seedling formation 150%, plant height 98.6%, the number of leaf 115,7%.  As the result yield of ginger rhizome increased by 400 % and phyloplan 598,3%, respectively, compared to the control.

Penapisan Isolat Rizobakteri dari Perakaran Tanaman Kedelai yang Sehat untuk Pengendalian Penyakit Pustul Bakteri (Xanthomonas axonopodis pv. glycines)

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 13, No 1 (2013): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

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Abstract

Screening of indigenous  rhizobacteria from healthy soybean root to control bacterial pustule (Xanthomonas axonopodis pv. glycines) using in planta technique. Plant growth promoting rhizobacteria are a group of bacteria  that actively colonize plant roots, increase plant growth and control  plant pathogens. The aim of this study was to obtain rhizobacteri isolates which have the ability to control bacterial pustule and  increase growth and yield of soybean. This method based on in planta selection of enhanced competitive soil root-colonizing bacteria from soil samples of healthy soybean root at endemic area of bacterial pustule in Darmasraya  District  and   Sijunjung District, West Sumatera. We characterized only the best rhizobacteri isolates which have ability to control bacterial pustule and to increase growth and yield of soybean. This type of characterization has possibility to find  new, easy and cheap biocontrol organisms. Ten Rhizobacteri isolates were introduced via seed treatment (108 cfu/ml) and soil drench to 3 week old  soybean seedling. Xanthomonas axonopodis pv. glycines were inoculated to one month old of soybean seedling. The effect of rhizobacteria  on disease incidence, disease severity, plant growth and yield of soybean were evaluated.  We have found that two selected  rhizobacteri isolates from soybean (P12Rz2.1 and P14Rz1.1) were the best isolates in promoting growth and the of the soybean plants  with the effectiveness  20.62 % and 20.47 %.

SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAH

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 13, No 2 (2013): September, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

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Abstract

Screening and identification of endophytic bacteria to control bacterial leaf blight disease on Shallot. The experiment was conducted in Laboratory and Green House, from January to June 2012.   Laboratory experiment consisted of three steps:  (1) isolation of endophytic bacteria from healthy onion roots, (2)In planta /screening of endophytic isolates capable of reducing bacterial leaf blight disease, and (3) molecular identification of potential endophytic isolates.  Treatments of  in planta test were arranged in Completely Randomized Design.  Collected isolates were tested for their capability in controlling bacterial leaf blight disease on shallot.  The variables observed were disease incidence, disease severity, and shallot yield. The results showed that out of 82 isolates successfully isolated, 56 isolates (68.29%) were Gram positive, and 26 isolate (31.71%) were Gram negative. All isolates were HR negative and pathogenicity negative. Six endophytic isolates showed  better performance in inducing resistance and increasing onion yield. Based on 16S rRNA sequence the six isolates were  Bacillus cereus strain P14 , Bacillus cereus strain Se07, Bacillus sp H1, Bacillus sp SJ1 and Serratia marcescens strain PPM4.