S. Gustina
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PENGARUH MEDIUM PEMISAH, PENAMBAHAN EKSTRAK KOPI SEBELUM PROSES PEMISAHAN SPERMATOZOA PEMBAWA KROMOSOM X DAN Y DAN LAMA PENYIMPANAN TERHADAP KUALITAS SEMEN CAIR KAMBING PERANAKAN ETTAWA Hasbi, -; Sonjaya, H.; Gustina, S.
JURNAL ILMU DAN TEKNOLOGI PETERNAKAN Vol 1, No 2 (2011)
Publisher : Fakultas Peternakan Universitas Hasanuddin

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Abstract

One problem in the application of biotechnology in spermatozoa sexing of X and Y sperm chromosome is a decrease in motility and percentage of live spermatozoa. The decrease could be caused by metabolic process, therefore spare energy obtained from separation medium is decreasing resulting in decrease in motility and percentage of live spermatozoa.  Research was carried out to evaluate the effects of separation medium, addition of coffee extract before sexing of X and Y sperm chromosome, and storage period on the quality of goat fresh semen.  This experiment was performed according to completely randomized design in factorial pattern (2 x 2 x 6). The first factor was the separation medium (A1: 10% and A2: 30%), the second factor was the coffee extract addition (B1: 0 mM and B2: 3 mM), and the third factor was the period of storage(D1: 0 hour, D2: 2 hours, D3: 4 hours, D4: 6 hours, D5: 8 hours and D6: 10 hours). The result showed that the motility of spermatozoa Y in 30% separation medium was higher (P<0.01) than that of spermatozoa X in 10% separation medium and it was higher (P<0.05) with addition of coffee extract compared with no coffee extract addition.  Viability of spermatozoa Y was higher (P<0.05) compared with spermatozoa X and addition of coffee extract not significantly different (P>0.05) compared with no coffee extract addition. The motility and viability of spermatozoa decreased during 10 hours storage. It was concluded that the motility and viability of spermatozoa Y was higher than spermatozoa X. Coffee extract can prevent a decrease of spermatozoa motility during storage. The motility of spermatozoa and the percentage of live spermatozoa decreased during storage.   Key words: Separation medium, Coffee extract, Storage period, Fresh semen, Ettawa cross goat
Effect of Separation Medium, Addition of Coffee Extract Before Sexing X and Y Sperm Chromosome and Storage Period on Quality of Fresh Semen of Ettawa Cross Goat Hasbi, .; Sonjaya, H.; Gustina, S.
Jurnal Ilmu dan Teknologi Peternakan Vol 1, No 2 (2011)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

One problem in the application of biotechnology in spermatozoa sexing of X and Y sperm chromosome is a decrease in motility and percentage of live spermatozoa. The decrease could be caused by metabolic process, therefore spare energy obtained from separation medium is decreasing resulting in decrease in motility and percentage of live spermatozoa.  Research was carried out to evaluate the effects of separation medium, addition of coffee extract before sexing of X and Y sperm chromosome, and storage period on the quality of goat fresh semen.  This experiment was performed according to completely randomized design in factorial pattern (2 x 2 x 6). The first factor was the separation medium (A1: 10% and A2: 30%), the second factor was the coffee extract addition (B1: 0 mM and B2: 3 mM), and the third factor was the period of storage(D1: 0 hour, D2: 2 hours, D3: 4 hours, D4: 6 hours, D5: 8 hours and D6: 10 hours). The result showed that the motility of spermatozoa Y in 30% separation medium was higher (P<0.01) than that of spermatozoa X in 10% separation medium and it was higher (P<0.05) with addition of coffee extract compared with no coffee extract addition.  Viability of spermatozoa Y was higher (P<0.05) compared with spermatozoa X and addition of coffee extract not significantly different (P>0.05) compared with no coffee extract addition. The motility and viability of spermatozoa decreased during 10 hours storage. It was concluded that the motility and viability of spermatozoa Y was higher than spermatozoa X. Coffee extract can prevent a decrease of spermatozoa motility during storage. The motility of spermatozoa and the percentage of live spermatozoa decreased during storage.
INSULIN-LIKE GROWTH FACTOR-I CONCENTRATION IN THE FOLLICULAR FLUID OF BALI CATTLE AND ITS ROLE IN THE OOCYTE NUCLEAR MATURATION AND FERTILIZATION RATE Hasbi, H.; Gustina, S.; Karja, N.W. K.; Supriatna, I.; Setiadi, M. A.
Media Peternakan Vol. 40 No. 1 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (625.199 KB) | DOI: 10.5398/medpet.2017.40.1.7

Abstract

The objective of this study was to determine the concentration of IGF-I in the follicular fluid (FF) of Bali cattle and its role in the nuclear maturation and fertilization rate. The follicular fluid was collected by the aspiration technique, then it was centrifuged at 1500 g for 30 min at 24oC. The supernatant was collected and stored at -20oC until being used in the experiment for analysis of IGF-1. A total of 1105 oocytes were used in this study. The oocytes were matured in M199 without supplementation of bovine serum albumin, with supplementation of BSA, and with supplementations of 10% FF (v/v) from the follicle with diameter Ø&lt;4 mm, 4?Ø&lt;6 mm, 6?Ø&lt;8 mm, and Ø?8 mm at the luteal phase and then fertilized. The results showed that the concentrations of IGF-I in the FF obtained during the luteal phase was significantly higher (P&lt;0.05) compared to those obtained during follicular phase. The IGF-I concentrations in the follicular fluid of follicle with diameter smaller than 6 mm were significantly higher (P&lt;0.05) compared to those with diameters larger than 6 mm. The percentage of nuclear maturation rate of oocytes cultured with FF obtained from follicle with diameter &lt;4 mm was significantly higher (P&lt;0.05) compared to those obtained from the other groups of follicle diameters. The supplementation of maturation media with BSA and FF were able to improve fertilization rate significantly (P&lt;0.05) compared to maturation media without BSA. In conclusion, the concentration of IGF-I in the follicular fluid obtained during the luteal phase was higher compared to those obtained during the follicular phase. The IGF-I concentrations in the follicular fluid of smaller follicles (diameter &lt;6 mm) were higher compared to those in the large follicles (diameter ?6 mm). The supplementation of FF can improve the nuclear maturation and fertilization rate
Effect of Separation Medium, Addition of Coffee Extract Before Sexing X and Y Sperm Chromosome and Storage Period on Quality of Fresh Semen of Ettawa Cross Goat Hasbi, .; Sonjaya, H.; Gustina, S.
Jurnal Ilmu dan Teknologi Peternakan Vol 1, No 2 (2011)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

One problem in the application of biotechnology in spermatozoa sexing of X and Y sperm chromosome is a decrease in motility and percentage of live spermatozoa. The decrease could be caused by metabolic process, therefore spare energy obtained from separation medium is decreasing resulting in decrease in motility and percentage of live spermatozoa.  Research was carried out to evaluate the effects of separation medium, addition of coffee extract before sexing of X and Y sperm chromosome, and storage period on the quality of goat fresh semen.  This experiment was performed according to completely randomized design in factorial pattern (2 x 2 x 6). The first factor was the separation medium (A1: 10% and A2: 30%), the second factor was the coffee extract addition (B1: 0 mM and B2: 3 mM), and the third factor was the period of storage(D1: 0 hour, D2: 2 hours, D3: 4 hours, D4: 6 hours, D5: 8 hours and D6: 10 hours). The result showed that the motility of spermatozoa Y in 30% separation medium was higher (P<0.01) than that of spermatozoa X in 10% separation medium and it was higher (P<0.05) with addition of coffee extract compared with no coffee extract addition.  Viability of spermatozoa Y was higher (P<0.05) compared with spermatozoa X and addition of coffee extract not significantly different (P>0.05) compared with no coffee extract addition. The motility and viability of spermatozoa decreased during 10 hours storage. It was concluded that the motility and viability of spermatozoa Y was higher than spermatozoa X. Coffee extract can prevent a decrease of spermatozoa motility during storage. The motility of spermatozoa and the percentage of live spermatozoa decreased during storage.