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PENGARUH MINUMAN BEROKSIGEN TERHADAP SISTEM IMUN, KADAR MALONALDEHIDA DAN PERFORMA RESPONDEN MAHASISWA OLAHRAGAWAN [Effect of Oxygenated Water on Immune System, Malonaldehyde Content and Student-Athlete Performance] Refdi, Cesar Welya; Zakaria, Fransiska Rungkat; Giriwono, Puspo Edi
Jurnal Teknologi Dan Industri Pangan Vol 25, No 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/8308

Abstract

PENGARUH MINUMAN BEROKSIGEN TERHADAP  SISTEM IMUN, KADAR MALONALDEHIDA DAN PERFORMA RESPONDEN MAHASISWA OLAHRAGAWAN [Effect of Oxygenated Water on Immune System, Malonaldehyde Content and Student-Athlete Performance] Cesar Welya Refdi, Fransiska Rungkat Zakaria* dan Puspo Edi Giriwono Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor   Diterima 23 Agustus 2013 / Disetujui 01 Juli 2014 ABSTRACT Exercise requires more oxygen input and cannot be fully obtained through breathing. The body has a homeostatic system, when more ATP is needed, the more oxygen is required by the body. Limited supply of oxygen such as those happens in athlete’s body automatically switches the respiration from aerobic into anaerobic. This situation results in building up of lactic acid which in turn lowers the energy for cellular metabolism as well asenergy for exercise. The purpose of this study was to assess the effects of short-term and long-term drinking of oxygenated water on lactic acid and energy expenditure (EE) in exercise performance; Cluster of Differentiation-4 (CD4), CD8, CD56, Interleukin-6 (IL-6) contents; and level of malonaldehyde. During short-term intervention, 12 male student volunteers drank oxygenated (50, 80 and 130 ppm) water and normal mineral water, 15 minutes before treadmill and after treadmill (10 Kmh). The lactic acid concentration was measured before and after treadmill, and 5 minutes after the reconsumption of the oxygenated water. During long-term intervention, 17 male student volunteers drank oxygenated (100 ppm) water twice a day for 21 days. The blood plasma and lymphocyte sampled before and after intervention were analyzed for the same parameters. The results showed that oxygenated water had no effect on lactic acid accumulations and EE. Similarlyt he long-term study showed that oxygenated water did not affect CD4 and CD8, had no harmful effect on IL-6 and malonaldehyde, yet it significantly increased the CD56 content.
SUPLEMENTASI MIKROENKAPSULAT EKSTRAK KULIT BUAH MANGGIS (KBM) MENURUNKAN KADAR MALONALDEHIDA HATI TIKUS Febriane, Nesya Nova; Giriwono, Puspo Edi; Koswara, Sutrisno; Prangdimurti, Endang
Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research) Vol 38, No 1 Jun (2015)
Publisher : Pusat Teknologi Terapan Kesehatan dan Epidemiologi Klinik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (434.993 KB)

Abstract

ABSTRACTHigh consumption of fried food contributes to increased risk of degenerative. Potent antioxidants that may alleviate this problem are contained in pericarp of mangosteen (KBM). However, its bitter taste hinders use of this antioxidant. Microencapsulation process can mask bitter taste and control the release of bioactive compounds. This study aims to evaluate the effectiveness of microencapsulated mangosteen pericarp extract in supressing malonaldehyde (MDA) in rat liver as a result of the consumption of oxidized palm oil. Antioxidants were extracted with methanol from KBM and microencapsulated using gelatin, carboxymethylcellulose (CMC) and maltodextrin. Its antioxidative capacity is determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Extract is supplemented to feed of rats at doses of 100mg/kg bw (KBM 1), meanwhile microencapsulated KBM at doses 100 (KBM 2) and 200 mg/kg bw (KBM 3) in addition to oxidized palm oil, for 50 days. After termination, liver was excised and liver MDA concentration was assayed. The decrease of MDAlevels on KBM 1, KBM 2, and KBM 3 respectively are 11.64 percent, 40.18 percent, and 53.43 percent. Supplementation of microencapsulated and non-encapsulated KBM extract do not affect body weights and feedconsumption of rats. Microencapsulated KBM is effective to reduce MDA levels significantly than its raw extract, in which 200 mg/kg bw is the best concentration. Its process can reduce the bitter taste of KBM.Keywords: antioxidant, mangosteen pericarp, oxidized oil, microencapsulation, liver MDA levelsABSTRAKTingginya konsumsi pangan yang digoreng meningkatkan resiko penyakit degeneratif. Salah satu antioksidan yang berpotensi mengatasi masalah ini adalah yang terkandung pada kulit buah manggis (KBM). Tetapi rasapahit dan getir yang terkandung dalam KBM menyebabkan penggunaannya menjadi terbatas. Oleh karena itu, digunakan proses mikroenkapsulasi yang dapat melindungi dan mengontrol pelepasan senyawa bioaktif yang terkandung dalam KBM. Penelitian ini bertujuan untuk mengevaluasi efektivitas mikroenkapsulasi ekstrak KBMuntuk menurunkan kadar malonaldehida (MDA) hati tikus percobaan yang mengonsumsi minyak sawit teroksidasi. Antioksidan diekstrak dari tepung KBM dengan metanol dan dimikroenkapsulasi menggunakan gelatin, karboksimetil selulosa (CMC), dan maltodekstrin. Kapasitas antioksidan diukur dengan metode 2,2- diphenyl-1-picrylhydrazyl (DPPH). Ekstrak dengan dosis 100 mg/kg bb (KBM 1) serta mikroenkapsulat KBMdengan dosis 100 (KBM 2) dan 200 mg/kg bb (KBM 3) disuplementasi pada pakan tikus dengan penambahan minyak sawit teroksidasi selama 50 hari perlakuan. Setelah diterminasi, hati tikus diambil lalu diukurkonsentrasi malonaldehida (MDA) yang terkandung. Penurunan kadar MDA pada KBM 1, KBM 2, dan KBM 3 yaitu sebesar 11,64 persen, 40,18 persen, dan 53,43 persen. Suplementasi ekstrak KBM yang dimikroenkapsulasi maupun tanpa enkapsulasi tidak mempengaruhi berat dan konsumsi pakan tikus. Mikroenkapsulat KBM efektif untuk menurunkan kadar MDA hati tikus dibandingkan ekstrak tanpa enkapsulasi, dimana konsentrasi terbaik yaitu 200 mg/kg bb. Proses ekstraksi dan mikroenkapsulasi dapat mengurangi rasa pahit dan getir KBM. [Penel Gizi Makan 2015, 38(1): 61-70]Kata kunci: antioksidan, kulit buah manggis, minyak sawit teroksidasi, mikroenkapsulasi, kadar MDA hati
PENGARUH MINUMAN BEROKSIGEN TERHADAP SISTEM IMUN, KADAR MALONALDEHIDA DAN PERFORMA RESPONDEN MAHASISWA OLAHRAGAWAN [Effect of Oxygenated Water on Immune System, Malonaldehyde Content and Student-Athlete Performance] Refdi, Cesar Welya; Zakaria, Fransiska Rungkat; Giriwono, Puspo Edi
Jurnal Teknologi dan Industri Pangan Vol 25, No 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2014.25.1.90

Abstract

PENGARUH MINUMAN BEROKSIGEN TERHADAP  SISTEM IMUN, KADAR MALONALDEHIDA DAN PERFORMA RESPONDEN MAHASISWA OLAHRAGAWAN[Effect of Oxygenated Water on Immune System, Malonaldehyde Content and Student-Athlete Performance]Cesar Welya Refdi, Fransiska Rungkat Zakaria* dan Puspo Edi GiriwonoDepartemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor Diterima 23 Agustus 2013 / Disetujui 01 Juli 2014ABSTRACT   Exercise requires more oxygen input and cannot be fully obtained through breathing. The body has a homeostatic system, when more ATP is needed, the more oxygen is required by the body. Limited supply of oxygen such as those happens in athlete?s body automatically switches the respiration from aerobic into anaerobic. This situation results in building up of lactic acid which in turn lowers the energy for cellular metabolism as well asenergy for exercise. The purpose of this study was to assess the effects of short-term and long-term drinking of oxygenated water on lactic acid and energy expenditure (EE) in exercise performance; Cluster of Differentiation-4 (CD4), CD8, CD56, Interleukin-6 (IL-6) contents; and level of malonaldehyde. During short-term intervention, 12 male student volunteers drank oxygenated (50, 80 and 130 ppm) water and normal mineral water, 15 minutes before treadmill and after treadmill (10 Kmh). The lactic acid concentration was measured before and after treadmill, and 5 minutes after the reconsumption of the oxygenated water. During long-term intervention, 17 male student volunteers drank oxygenated (100 ppm) water twice a day for 21 days. The blood plasma and lymphocyte sampled before and after intervention were analyzed for the same parameters. The results showed that oxygenated water had no effect on lactic acid accumulations and EE. Similarlyt he long-term study showed that oxygenated water did not affect CD4 and CD8, had no harmful effect on IL-6 and malonaldehyde, yet it significantly increased the CD56 content.  
PARAMETER KINETIKA INAKTIVASI TERMAL DAN ISOLASI Staphylococcus aureus PADA MINUMAN DARI GEL CINCAU HIJAU DAN ROSELA Purnomo, Eko Hari; Giriwono, Puspo Edi; Indrasti, Dias; Firlieyanti, Antung Sima; Kinasih, Andini Giwang
Jurnal Teknologi dan Industri Pangan Vol 26, No 1 (2015): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2015.26.1.124

Abstract

Information about heat resistance (D and z values) of target bacteria is needed for the thermal process design on drink from Green Grass Jelly (Premna oblongifolia Merr.) and Roselle (Hibiscus sabdariffa L.), so it can guarantee quality and safety of the product. The objectives of this research were to isolate Staphylococcus aureus from commercial green grass jelly and to determine the D and z values of Staphylococcus aureus (from commercial product and standard clinical isolate ATCC 25923) on heating menstruum of green grass jelly and roselle. Isolation of S. aureus was done by inoculation in selective medium, D and z values of Staphylococcus aureus were assessed by heating at constant temperatures of 57, 53, 49, and 45°C during the time interval 2.5, 5, 10, and 15 minutes. The results showed that one isolate (Isolate A) gave positive response of Staphylococcus aureus in isolation tests and had similar percentage with the reference culture of 41.8% using the API Staph Kit. Heat resistance of Staphylococcus aureus (represented as D value) isolated from green grass jelly at constant heating temperature of D45, D49, D53 and D57 were 32.3, 17.9, 4.6, and 1.5 minutes. On the other hand, D value of isolates ATCC 25923 (standard clinical isolate) at constant heating temperature of D45, D49, D53 and D57 were 18.5, 6.8, 2.9, and 1.4 minutes. The z value of isolates A and ATCC 25923 were 8.8°C and 10.8°C. Smaller z value of isolate A showed that pasteurization process can be accelerated and optimized with increasing the temperature slightly but has the same lethality effect.
SENYAWA BIOAKTIF TORBANGUN (Plectranthus amboinicus (Lour.) SPRENG) FRAKSI KLOROFORM INDUKSI APOPTOSIS PADA KANKER PAYUDARA (SEL MCF-7) IN VITRO Yulianto, Wahid; Andarwulan, Nuri; Giriwono, Puspo Edi; Pamungkas, Joko
Majalah Obat Tradisional Vol 22, No 1 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.642 KB) | DOI: 10.22146/tradmedj.24312

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Tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng) telah dimanfaatkan secara tradisional pada berbagai negara di daerah tropis untuk menyembuhkan berbagai penyakit. Penelitian ini bertujuan untuk mengidentifikasi komponen aktif pada fraksi kloroform dari ekstrak tanaman torbangun tersebut yang memiliki efek terhadap ekspresi gen-gen yang berkaitan dengan apoptosis pada sel kanker payudara MCF-7. Apoptosis diamati secara morfologis menggunakan metode pewarnaan inti sel Hoechst. Ekspresi gen dianalisa menggunakan Real-Time PCR sedangkan komponen bioaktif diidentifikasi menggunakan LC-MS. Hasil pengamatan morfologi secara jelas memperlihatkan terjadinya apoptosis pada sel setelah diberi perlakuan ekstrak tanaman. Terjadi peningkatan ekspresi gen anti-apoptosis Bcl-2 namun peningkatan tersebut tidak mampu mencegah terjadinya apoptosis pada sel. Ekspresi gen p53 dan p21 meningkat secara signifikan setelah diberi perlakuan fraksi kloroform. Ekspresi gen caspase 9, caspase 7 dan caspase 1 meningkat dengan level peningkatan yang dipengaruhi oleh konsentrasi yang diberikan. Sebagian besar komponen yang teridentifikasi tergolong dalam kelompok diterpenoid yang kemungkinan berkontribusi terhadap bioaktifitas fraksi kloroform dalam menginduksi apoptosis pada sel kanker MCF-7.
Enterococcus faecium 1.15 Isolated from Bakasam Showed Milk Clotting Activity Putranto, Wendry Setiyadi; Suradi, Kusmajadi; Chairunnisa, Hartati; Mustopa, Apon Zaenal; Giriwono, Puspo Edi; Kusumaningrum, Harsi Dewantari; Suhartono, Maggy Thenawidjaja
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (499.913 KB) | DOI: 10.14203/ab.v21i1.293

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The Lactic Acid Bacteria with Milk Clotting Activity (MCA) were isolated from Bakasam, an Indonesian traditional fermented meat. The isolate screening was carried out using modified method of Skim Milk Agar and Milk Clotting Activity Test, and the isolate was then identified using 16S rRNA. We found 4 isolates that showed MCA of 18-20 SU/ml. Identification using 16S rRNA indicated that the isolate ALG.1.15 was 99% (FR3-F primer) and 99% (FR3-R primer) identic with Enterococcus faecium. The isolate potentially produced renin-like protease to subtitute renin from veal.  
The Development of Protein Extract from Local Indonesian Shrimp (Penaeus merquiensis) for Food Allergies Reagent Using Skin Prick Test Method CHALID, SRI YADIAL; SYAH, DAHRUL; GIRIWONO, PUSPO EDI; ZAKARIA, FRANSISKA RUNGKAT
JURNAL ILMU KEFARMASIAN INDONESIA Vol 13 No 2 (2015): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Jerbung is a kind of shrimp which widely spread in Indonesian marine that is a major allergen from seafood origin. Until now, the best treatment for food allergies is avoid from all kinds of food allergens. Avoiding certain foods should be established based on allergy tests such as skin prick test (SPT) and double-blind placebo-controlled food challenges. SPT is the most widely used test for detecting IgE-mediated food allergies. The main ingredient of SPT are protein extract which is called the reagent. Jerbung protein was extracted from part of meat by buffer phosphat saline (PBS) then analyzed by using SDS-PAGE and protein concentration was analyzed by Bradford assay. Protein extract was formulated to SPT reagent based on standarization of the European Pharmacopoeia Monograph on Allergen Products 7 (2010:1063). Skin prick test reagent was applied on 24 adult participants who were food allergies and 16 adults individuals of non food allergies. Sera of subjects were collected to measuretotal IgE and specific IgE-binding. The sensitivity of protein extract was 95% and negative error of 5%. Whereas specificity was 94% with a positive error was 6%. Immunobloting was also performed on 10 sera subjects. Nine sera subjects of these shrimp allergic participants showed the specific binding to allergen of jerbung shrimp with the range of molecular weight of 28-63 kDa.
Profil Dan Sensitivitas Protein Alergen Ikan Tongkol (Thunnus albacares) Sebagai Reagen Skin Prick Test (SPT) Chalid, Sri Yadial; Syah, Dahrul; Giriwono, Puspo Edi; Rungkat, Fransiska; Zakaria, Zakaria
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 5, No. 1, May 2019
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Syarif Hidayatullah State Islamic Uni

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v5i1.9678

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Ikan tongkol merupakan salah satu produk laut penyebab alergi makanan. Gejala klinis reaksi alergi makanan adalah gatal, bentol, bengkak, sesak nafas, batuk, dan yang terparah adalah syok anafilaksis. Pengobatan dan pencegahan alergi makanan yang terbaik adalah menghindari konsumsi penyebab alergi. Menghindari konsumsi suatu makanan sebaiknya berdasarkan uji alergi seperti Skin Prick Test (SPT). SPT dilakukan menggunakan reagen SPT yang dicukitkan pada lapisan epidermis kulit lengan bagian volar. Reagen alergen pada penelitian ini merupakan protein ikan tongkol yang berasal dari laut Indonesia dan diekstrak dengan buffer fosfat, Profil ekstrak protein menggunakan elektroforesis dan immunoblotting untuk menentukan protein allergen. Sebanyak 15 pita protein terdeteksi pada ekstrak ikan tongkol yaitu protein dengan berat molekul antara 17 sampai 152 kDa. Potensi alergenik terdapat pada pita protein dengan berat molekul antara 12 sampai 50 kDa. Reagen SPT ikan tongkol mempunyai sensitivitas sebesar 81.8% dan spesifitas 100% sehingga disimpulkan bahwa reagen ekstrak ikan tongkol dapat digunakan sebagai reagen skin prick test Kata kunci: Alergi, protein, tongkol, gejala klinis, skin prick test   Tuna fish is one of the marine products that can cause allergic. Clinical symptoms of allergic are a bump, swelling, shortness of breath, coughing and anaphylactic shock is the worst symptom. The best medication and treatment is avoiding the consumption of food that could cause allergy. Avoiding consumption of food should be based on an allergy test such as a Skin Prick Test (SPT). SPT using reagent which is applied on the skin of the forearm between the wrist and elbow. The reagent for SPT is a protein solution of tuna which was extracted by phosphate buffer then protein profile was detected using electrophoresis. Immunoblotting was done to determine the molecular weight of the allergen protein. Fifteen protein bands were detected on tuna fish extract; the molecular weight of the protein was obtained between 17 to 152 kDa. Protein allergenic are molecules that have protein bands with a molecular weight between 12 and 50 kDa. Tuna fish extract solution for SPT had a sensitivity is 81.8%, and specificity is 100%, it was concluded that tuna fish reagent could be used as SPT reagent. Keywords: Allergen, protein, tuna fish, skin prick test.
Role of Nutrition and Health Claims on Growing Up Milk Product as Purchasing Decision Determination RTR, Ayu Puspitalena; Palupi, Nurheni Sri; Giriwono, Puspo Edi
Jurnal Mutu Pangan (Indonesian Journal of Food Quality) Vol 2, No 2 (2015)
Publisher : Jurnal Mutu Pangan (Indonesian Journal of Food Quality)

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Abstract

Abstract. . This moment,it has not been known the role of nutrition and health claims as purchasing decision determination. Objective of the research is to study the role of nutrition and health claims on the labels of growing up milk as purchasing decision and to analyze corellation of respondent's characteristics which have influence. Study was conducted by survey, data were analyzed descriptively and statistically. Label of growing up milk for children 1-3 years old read by 70% respondent and 30% occasionally, and 23% of respondents do not read nutrition and health claims. Nutritional and health claims have a role as a determinant of purchasing decisions for consumers of 24%. As for the 76% purchase decision is influenced by other factors such as suitable with a child, nutrient content, good taste or price. The role of nutritional and health claims as a determinant of purchase decisions are influenced by factors of education, family expenses per month and expenditure for milk per month. As for the other factors that include age, occupation, number of children, place of purchase, and frequency of purchases did not affect the role of nutrition and health claims as a determinant of purchase. Keywords: Label, growing up milk, nutrition and health claims, purchasing decisions Abstrak. Saat ini belum diketahui peranan klaim gizi dan kesehatan sebagai penentu keputusan pembelian. Penelitian bertujuan mengkaji peranan klaim gizi dan kesehatan pada label produk susu pertumbuhan terhadap keputusan pembelian dan menganalisis hubungan karakteristik responden yang berpengaruh. Penelitian dilakukan dengan metode survei, data dianalisis secara deskriptif dan statistik. Sebanyak 70% responden membaca label produk susu pertumbuhan untuk anak usia 1-3 tahun dan 30% kadang-kadang, serta 23% responden tidak membaca klaim gizi dan kesehatan. Klaim gizi dan kesehatan mempunyai peranan sebagai penentu keputusan pembelian bagi konsumen yaitu sebesar 24%. Sebesar 76% keputusan pembelian dipengaruhi oleh faktor lainnya seperti cocok dengan anak, kandungan gizi, rasa yang enak ataupun harganya. Peranan klaim gizi dan kesehatan sebagai penentu keputusan pembelian dipengaruhi oleh faktor pendidikan, pengeluaran keluarga per bulan dan pengeluaran untuk susu per bulan. Faktor lain yang meliputi usia, pekerjaan, jumlah anak, lokasi pembelian, dan frekuensi pembelian tidak mempengaruhi terhadap peran klaim gizi dan kesehatan sebagai penentu pembelian. Kata kunci: Label, susu pertumbuhan, klaim gizi dan kesehatan, keputusan pembelian Aplikasi Praktis: Klaim gizi dan kesehatan pada label susu pertumbuhan untuk anak usia 1-3 tahun berperan sebagai penentu keputusan pembelian. Namun demikian belum semua responden membaca label serta klaim gizi dan kesehatan karena kurang dapat dipahami. Hasil ini dapat digunakan sebagai bahan pertimbangan bagi badan regulasi untuk mengatur klaim gizi dan kesehatan dalam bahasa yang lebih dapat dimengerti oleh masyarakat. Bagi produsen bermanfaat untuk dapat menentukan klaim gizi dan kesehatan yang tepat pada label sehingga berperan efektif sebagai pertimbangan dalam memilih produk. http://jurnalmutupangan.com/index.php?link=view&id=JMP-02-17-037
Prevalensi Penyakit Kanker di Indonesia Berdasarkan Pola Konsumsi Pangan dan Gaya Hidup (Cancer prevalence in Indonesia Based on Food Consumption Patterns and Lifestyle) Aliwikarta, Kuswanto; Palupi, Nurheni Sri; Giriwono, Puspo Edi
Jurnal Mutu Pangan (Indonesian Journal of Food Quality) Vol 3, No 1 (2016)
Publisher : Jurnal Mutu Pangan (Indonesian Journal of Food Quality)

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Abstract

Consumption of processed meat is associated to cancer risk increasing. This study used secondary data from RISKESDAS 2013 with a case-control study design, is aimed to analyze the cancer prevelance in Indonesia based on food consumption patterns and lifestyles. The case population is 90 people suffering from cancer and 90 people with no cancer randomly selected as control. Respondent profiles were analyzed by univariate analysis. The relationship of cancer risk factors were analyzed by bivariate analysis and determination of risk factors using multivariate analysis. Sensitivity and specificity analyses showed the cancer vulnerable age is ≥46 years. Processed meat consumption is associated significantly (p=0.000; OR=1.25) in the cancer prevelance. Respondents frequently eat processed meat risk is predicted 1.25 times compared to those not taking. Vegetable consumption (p=0.046; OR=0.509) and fruit consumption (p=0.022; OR=0.365) related significantly to cancer. Vegetables consumption reduce the risk by 0509 times, and fruit consumption reduce the risk by 0365 times. Lifestyle risk factors (physical activity and smoking) significant (p=0.000) to cancer. Multivariate analysis results showed the age, gen- der and processed meat consumption is associated significantly (p=0.000) to cancer.