Herry Garna
Department of Child Health, Faculty of Medicine, Padjadjaran University, Bandung

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Journal : Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)

EKSPRESI KORESEPTOR HUMAN IMMUNODEFICIENCY VIRUS CCR5 DAN CXCR4 PADA SUBSET SEL LIMFOSIT T SERTA MONOSIT Rengga Indrati, Agnes; Meijerink, Hinta; Garna, Herry; Alisjahbana, Bachti; Parwati, Ida; van Crevel, Reinout; van der Venn, Andre
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 2 (2012)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i2.1012

Abstract

Chemokine receptors CCR5 and CXCR4 which lied on lymphocyte cell surface play important role in HIV infection and pathogenesis.The expression of these chemokine receptors will affect progressively the disease. The objectives of the study are to find the distributionof lymphocyte T cell subset and monocyte among the peripheral blood mononuclear cells and to know the determination of CCR5 andCXCR4 co receptors expression on T lymphocyte cells subset and monocyte. This study is a preliminary study to explore the distributionof co receptors CCR5 and CXCR4 expression in healthy people. The sample taken is peripheral blood mononuclear cells (PBMC) fromhealthy subjects. The identification of T lymphocyte cells subsets and monocyte, and the expression of CCR5 and CXCR4 co receptorswere determined using flowcytometry. The memory T cell (CD4+CD45RO) is found to be the largest proportion among T lymphocyte cell(66.2%), whereas the other T lymphocyte cell subset, regulatory T cell, which identified by CD25+ high expression was found between2.0-5.3% from the whole T lymphocyte cell. The proportion of CXCR4 co receptors was found higher compare to CCR5 co receptorson all T lymphocyte subsets and monocyte. Only small proportion of monocyte expresses both co receptors (2.85%), but most of the Tlymphocyte cell expressed both CCR5 and CXCR4. The expression of the CXCR4 on regulatory T cell (18.18%) is the lowest compared toother cells, but the fluorescence intensity of both co receptors was very high (CCR5 53.53 and CXCR4 49.33). The different distributionof CCR5 and CXCR4 co receptors among T lymphocyte cell subsets and monocyte will influence the vulnerability and the pathogenicityof HIV infection.
Co-Authors Achmad Surjono, Achmad Adjat Sedjati Rasyad Agnes Rengga Indrati Agus Firmansyah Ahmad Suardi, Ahmad Alex Chairulfatah Ani Melani Maskoen Aniceto Cardoso Barreto, Aniceto Cardoso Ardini Saptaningsih Raksanagara, Ardini Saptaningsih Ari Indra Susanti, Ari Indra Arief Budiman Aulia Fitri Swity, Aulia Fitri Azhali M. S., Azhali M. Bachti Alisjahbana Budi Setiabudiawan Cissy B. Kartasasmita Dadang Hudaya S, Dadang Hudaya Dany Hilmanto Dedi Rachmadi Dessy Afrianti, Dessy Dida A. Gurnida Dida Akhmad Gurnida Dina Garniasih, Dina Djatnika Setiabudi Dwi Prasetyo Dzulfikar D. Lukmanul Hakim Eddy Fadlyana Endah Pujiastuti, Endah Fina Meilyana Firman F. Wirakusumah Firman Wirakusumah, Firman Fiva A Kadi, Fiva A Gartika Sapartini Harry Iskandar, Harry Heda Melinda D. Nataprawira Henne Giyantini, Henne Herman, Herry Hidayat Wijayanegara Hudaya, Dadang Husin, Farid Ida Parwati Irawan, Gaga Irvan Afriandi Ismawaty, Nenden Iwin Sumarman Iwin Suwarman Julistio Djais, Julistio Julistio T. B. Djais, Julistio T. B. Julistio T.B. Djais, Julistio T.B. Junia, Jujun Kamila, Nurul Auliya Kusnandi Rusmil Laili, Fauzia Linda Marlina, Linda Lony Novita, Lony M.S. Azhali Maryamah, Aam Meijerink, Hinta Mohamad Yanuar Anggara Nanan Sekarwana Novila Sjafri Bachtiar Novilia Sjafri Bachtiar Nur Melani Sari, Nur Melani Nurakilah, Heni Okinarum, Giyawati Yulilania Ponpon Idjradinata Ponpon S Idjradinata R.M Ryadi Fadil, R.M Ryadi Rahmat Budi Reinout van Crevel Rengga Indrati, Agnes Reni Ghrahani Retno Saraswati Sitomorang, Ingrid Rita Sjarif Hidajat, Sjarif Soenarjati Soedigo Adi Soepriadi, Myrna Sri Endah Rahayuningsih Sugih, Siti Sunjaya, Deni K. Susiarno, Hadi Susiarno, Hadi Sutisna, Ma'mun Tina Ramayanthi, Tina Tisnasari Hafsah, Tisnasari Titik Respati Tono Djuwantono van der Venn, Andre Widajanti, Endang Wiwin Winiar