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Journal : TORANI

Frekuensi Pemberian Pakan Buatan Berbasis Limbah Untuk Produksi Kepiting Bakau Cangkang Lunak Aslamyah, Siiti; Fujaya, Yushinta
TORANI : Jurnal Ilmu Kelautan dan Perikanan Vol 24, No 1 (2014)
Publisher : TORANI : Jurnal Ilmu Kelautan dan Perikanan

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Abstract

Feed eficiency is largely determined by its availability in suficient quantities and on timefeeding, s o it is necessary to study the frequency of feeding for the best production of soft shell crab. Four feeding frequency (1 time per day, 1 time per 2 days, 1 time per 3 days, and 1 time per 4 days) tested in this study. Feed with nutrient composition of 30.86% protein, 7.2% fat, nitrogen free extract (NFE) 48.89%, crude fiber 5.7% enriched with vitomolt 0.1041 5 mg/g of feed to the standard dose and 0.4166 mg vitomolt/g feed to high doses; or equal to 700 ng vitomolt/g  crab. Test crabsof Scylla  sp. reared in  crabs box and placed in  the pond. The results showed  the frequency of feeding 1 time per 2 days resulted in the highest percentage of molting (66.67%) and the lowest percentage of moulting was found at 1 per 4 times a day of feeding (36.67%). Growth param eters such as weight and carapace width, and feed eficiency did not difer among all treatments. Thus, feeding in the soft shell crab for the maximum production can be done with a frequency of 1 time per 2 days .
Effectiveness of Addition Papain Enzyme in Artificial Diet on the Metamorphosis Rate and Glycogen Content of Mangrove Crab Larvae (Scylla olivacea) Haryati, Haryati; Fujaya, Yushinta; Saade, Edison; Fajrianti, Dwi
TORANI: Journal of Fisheries and Marine Science Vol 1, No 2 (2018)
Publisher : Marine Science and Fisheries Faculty, Hasanuddin University

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Abstract

In the rearing of fish larvae, artificial feed can be given when digestive enzymes have been produced. To improve the ability of fish larvae to utilize artificial feeds can be done with the addition of exogenous enzyme into the artificial feeds. The aim of this study was to determine the dose of papain enzyme and the correct predigest artificial feeding stadium on metamorphosis rate and glycogen content of mangrove larvae (Scylla olivacea). The experimental design used was a factorial pattern with a complete randomized baseline design, the first factor being the dose of papain enzyme (0%, 1.5%, 3.0% and 4.5%), while the second factor was the predigest artificial feeding stage (zoea 2 and 3).  The results showed that the difference of dose of papain enzyme and predigest artificial feeding stages had significant effect (p <0.05) on the metamorphosis rate of  7, 9, 10, 12, 13, 16 and 17 day larvae, but the interaction between them was not significant (p>0,05). The rate of metamorphosis at doses of enzyme papain was 0% and 1.5% longer and significantly different (p <0.05) compared to 3% and 4.5% doses. The rate of metamorphosis at doses of 3.0% and 4.5% was not significantly different (p> 0.05). The content of glycogen larvae in dose of enzyme papain 4,5% not different than dose 1,5% and 3,0% but higher and significantly different than dose 0%. Glycogen content at dose 0%, 1,5% and 3,0%. was not significantly different (p> 0.05). Based on the results of these studies to hydrolyze feed proteins can be used papain enzyme with a dose of 4.5%.Keywords: papain enzyme, metamorphosis rate, mangrove crab larvae (Scylla olivacea), glycogen content
Effectivity of mulberry leaf extract on stimulating ekdisteroid hemolimph content and molting of mud crab (Scylla olivacea) Fujaya, Yushinta; Trijuno, Dody Dharmawan; Haryati, Haryati; Hasnidar, Hasnidar; Rusdi, Muhammad; Usman, Zainal
TORANI: Journal of Fisheries and Marine Science Vol 2, No 1 (2018)
Publisher : Marine Science and Fisheries Faculty, Hasanuddin University

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Abstract

This study aims to study the performance of phytoecdisteroids from mulberry leaves in molting stimulating of mangrove crabs. The research was carried out at the Research and Development Center for Aquaculture at the Faculty of Marine Sciences and Fisheries at Hasanuddin University, located in Bojo Village, Mallusetasi District, Barru Regency, South Sulawesi Province. Mangrove crabs (Scylla olivacea) weighing 45-55 g and carapace widths 60-65 mm were used as test animals. There were five doses of mulberry leaf extract applied through feed, namely: A) 1.1 mg / g feed, B) 1.9 mg / g feed, C) 2.7 mg / g feed, D) 3.5 mg / g feed. Crabs were kept individually in plastic boxes that are floated above the surface of the pond. During rearing, crabs are fed 3% dry fish per day which has been enriched with mulberry leaf extract. The parameters observed were ecdysteroid compounds contained in mulberry leaf extract, ecdysteroid content in hemolymph before and after application of mulberry leaf extract, and molting percentage. Identification of the ekdisteroid compounds contained in mulberry leaf extract was carried out using Thin Layer Chromatography (TLC) and Measurement of the content of ecdysteroids in crab hemolymh was carried out using Ultra Fast Liquid Chromatography (UFLC).  The results showed that the 3.5 mg dose of mulberry leaf extract / g feed gave the highest increase in ecdysteroid hemolymph, which was approximately 1760 ekdisteroid per mL hemolimph, while the dose of 1.1 mg / g feed only provided an increase of 100 ng ekdisteroid per mL hemolymph. The higher the dose of mulberry leaf extract in the feed, the higher the increase in the concentration of hemolymph ecdysteroids, but the high concentration of ecdystoid in the hemolymph does not guarantee molting.  The optimal dose of mulberry leaf extract in the feed stimulating molting was 2.4 mg / g of feed.Keywords: mulberry, phytoecdysteroid, molting, crab, aquaculture