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Journal : Aquacultura Indonesiana

Acceleration of Blue Swimming Crab (Portunus pelagicus) Larval Development by Phytoecdysteroid Ahmad, Fakhirah; Fujaya, Yushinta; Trijuno, Dody Dharmawan; Aslamyah, Siti
Aquacultura Indonesiana Vol 16, No 2 (2015): Volume 16 Issue 2 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.259 KB) | DOI: 10.21534/ai.v16i2.9

Abstract

Phytoecdysteroid is a mimic hormone like molt regulating hormone in arthropoda and crustacea.  This research aims to investigate the response of Portunus pelagicus larvae on various doses of vitomolt enrichment in predigested artificial diet.  Four doses of vitomolt (0; 4; 40; 400 mg/100 g artificial diet) were used in this research.  Larval development and survival rate were measured The results showed that the larvae development rate was increased in congruent with the dose of vitomolt.  However, highest survival rate was found on 4 mg vitomolt/100 g artificial diet (P>0.05).  Based on regression analysis, it is estimated the optimal dose of vitomolt for larval development rate and survival rate was about 23 mg/100 g of artificial diet. Vitomolt application in artificial diet could be used to accelerate the larval development rate in blue swimming crab. Keywords: Portunus pelagicus, phytoecdysteroid, artificial diet, survival, larval development
The Morphometric Character and Mitochondrial 16S rRNA Sequence of Portunus pelagicus Fujaya, Yushinta; Hidayani, Andi Aliah; Asphama, Andi Ivo; Trijuno, Dody Dharmawan; Tenriulo, Andi; Parenrengi, Andi
Aquacultura Indonesiana Vol 16, No 1 (2015): Volume 16 Issue 1 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.576 KB) | DOI: 10.21534/ai.v16i1.1

Abstract

Blue swimming crab (Portunus pelagicus) colors have variations from different habitats and they have different vitality. The purpose of this study was to analyze the diversity of the P. pelagicus using morphometric and 16SrRNA mitochondrial sequence.  There were three groups of blue swimming crab characterized by differences in color and density patterns of spots.  The result of Canonical discriminant analysis showed that there were morphometric differences among the three groups.  The main distinguishing morphometric characteristics are the ratio of carapace width and length as well as the ratio of the length and width of cheliped merus.  The third group of crab is also has similarity about 98-99% by the Portunus pelagicus 16S rRNA sequences available in gene banks.  These studies had concluded that differences in color and pattern of the spot density have also morphometric and genetic differences. At least there were two groups based on genetic variation of Portunus pelagicus from Barru Regency waters which may have a variety of different properties.  Further research is needed to study the adaptation abilities of the two groups of crab on the condition of aquaculture as a potential domestic animal.Keywords: Crab; Portunus pelagicus; Morphometric; 16S rRNA.
EFFECT OF ROTIFERS (Branchionus plicatilis) REPLACEMENT WITH PREDIGESTED ARTIFICIAL FEED ON SURVIVAL AND LARVAE DEVELOPMENT OF BLUE SWIMMING CRAB (Portunus pelagicus) Sudaryono, Agung; Alik T, Widyawati; Aslamyah, Siti; Fujaya, Yushinta
Aquacultura Indonesiana Vol 16, No 2 (2015): Volume 16 Issue 2 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.087 KB) | DOI: 10.21534/ai.v16i2.17

Abstract

One of the problems faced in blue swimming crab hatchery business is the reliance on the use of natural feed. This research aimed to study the effect of rotifer feed replacement by predigested artificial feed on survival rates, growth rates of the larval stages and to determine the best feeding period of predigest artificial feed 100% to replace rotifers. The study was conducted in May-June 2015 in a household scale hatchery location at Bojo Village, Mallusettasi District, Kabupaten of Barru. There were five replacements of rotifers by predigest artificial feed namely: A. Control (feeding with rotifer up to stadia zoea 4), B (feeding with rotifer up to stadia zoea 3), C (feeding with rotifer up to stadia zoea 2), D (feeding with rotifer only at stadia zoea 1), E (No feeding with rotifer). Predigest artificial feeds were fed for a rearing period of larvae in all treatments. The results showed that the survival of the blue crabs larvae that did not consume rotifers as a feed source were significantly lower than the crab larvae consuming rotifers. The larvae with no feeding rotifer survived only at stadia zoea-2 while those feeding with rotifers survived until zoea-4. The rate of stadia changes did not differ among treatments getting rotifers. Use of predigest artificial feed on crab hatchery can be an alternative to rotifers in the future. Feeding predigest 100% can be started at stadia zoea-2. Key words: blue swimming crab, Portunus pelagicus, larvae, rotifers, predigest artificial feed.
The Intracellular Cryoprotectant Effects in Preserving Goramy Spermatozoa after Two Days Sub-Zero Freezing Abinawanto, Abinawanto; Fitrianingrum, Nisa; Lestari, Retno; Sudaryono, Agung; Rostika, Rita; Fujaya, Yushinta
Aquacultura Indonesiana Vol 16, No 1 (2015): Volume 16 Issue 1 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (100.86 KB) | DOI: 10.21534/ai.v16i1.2

Abstract

The spermatozoa quality of goramy two days after sub-zero freezing was examined. The quality of spermatozoa examined included motility, viability, and abnormality. We aimed to determine the optimum concentration of glycerol protecting spermatozoa during preservation. We used 0%, 1%, 3%, 5%, 7%, and 9% of glycerol, respectively.  Sperms were diluted by the combination of glycerol and fish ringer (1 part of sperm + 3 part of solvent). The dilute sperms were then equiliberated at 4°C for 45 min, and were freezed at -34°C for two days. Thawing was then carried out at 30°C for 2 min. Based on Dunnet test, 5% of glycerol was the optimum concentration maintaining spermatozoa motility (75.95±4.76)%.Keywords: glycerol; Osphronemus goramy; spermatozoa motility, viability, and abnormality; sub-zero freezing
Effect of Predigested Artificial Diet Using Papain Enzyme on the Degree of Protein Hydrolysis and Protease Enzyme Activity of Mud Crab (Scylla olivacea) Larvae at Zoea 2 and 3 Stages Haryati, Haryati; Fujaya, Yushinta; Saade, Edison
Aquacultura Indonesiana Vol 19, No 2 (2018)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.33 KB) | DOI: 10.21534/ai.v19i2.118

Abstract

The ability of mud crab (Scylla olivacea) larvae to digest artificial diet depends on the availability of digestive enzyme. To enhance the larvaes capability to utilize artificial diet can be conducted by adding exogenous enzyme to the diet.The aim of this research was to determine the dose of papain enzyme and stadia of predigest artificial diet that produced the best degree of protein hydrolysis and protease enzyme activity. Completely randomized designwas used to evaluate the effect of papain enzyme dose on the degree of protein, with four treatment and three replications, namely the doses of 0.0%, 1.5%, 3.0%, and 4.5%. Factorial pattern with the completely randomized design was used to evaluate the effect of papain enzyme dose and the proper predigest artificial feeding stage on the activity of protease enzyme. The first factor was the papain enzyme dose (0.0%, 1.5%, 3.0%, and 4.5%), while second factor was the stadia of larvae when it is fed with predigested artificial diet (zoea 2 and zoea 3 stadia). The research showed that the difference of papain enzyme dose provides real effect on degree of protein hydrolysis either on JP 0 or JP 1 diet brands (P<0.05). Degree of protein hydrolysis of the diet predigested with 0.0% papain enzyme was the lowest and has real difference (P<0.05) with the 1.5%, 3.0% and 4.5% doses. Degree of protein hydrolysis on 3% dose of papain enzyme does not provide real difference with the 4.5%, namely  19.753% and 22.890% respectively in JP 0, 18.707 % and 20.430 % in JP 1, but has real difference (P<0.05) with 1.5% doses.  The difference papain enzyme dose and  stadia of predigest artificial diet as well as the interaction between both of them had significant effect  (p<0.05) on the activity of protease enzyme. On the larvae of zoea 2 stadia, the activity of protease enzyme on the larvae fed with predigest artificial diet starting from zoea 2 stadia with 3.0% and 4.5% papain enzyme dose or those still fed with natural diet does not provide real difference, but is higher and provides real difference than 0% and 1.5% doses. On the larvae of zoea 3 stadia, the activity of protease enzyme to the larvae fed with artificial diet starting from zoea 2 with 0% and 1.5% papain enzyme doses, and those fed with artificial diet starting from zoea 3 stadia with 0% papain enzyme dose does not provide real difference (p>0.05) but is lower and provides real difference than other treatments. Protease enzyme activity on the larvae  fed with predigest artificial diet using  4.5%  papain enzyme  starting at zoea 2 stadia ,  was not significantly different (P <0.05) compared to larvae fed artificial diet which was  predigest  with 0.0%,  1.5%, 3.0 and 4.5  papain enzyme started at Zoea 3.  Based on the degree of protein hydrolysis, papain enzymes can be used to hydrolyze artificial diet  in doses ranging from 3.0% to 4.5%. Based on the activity of protease enzymes, mud crab larvae (Scylla olivacea) predigested artificial diet using 4.5% papain enzyme can be provided to the larvae starting from zoea 2 stadia.
Quality of Blue swimming crab Portunus pelagicus Larvae from Domesticated Broodstock Trijuno, Dody Dharmawan; Fujaya, Yushinta; Agviranti, Agviranti; Marhama, Syamsurya
Aquacultura Indonesiana Vol 16, No 1 (2015): Volume 16 Issue 1 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.051 KB) | DOI: 10.21534/ai.v16i1.5

Abstract

Blue swimming crab (Portunus pelagicus) is the important world fishery resource, but the crab larval rearing faces high mortality problem. The aim of the research was to compare survival and growth rate of larvae resulted from wild and domesticated broodstock blue swimming crab. Domesticated and wild broodstock were used to produce larvae. Domesticated broodstock was selected from repeated reared crab until third generation. Selection of the broodstock mainly based on the survival and growth rate. The wild broodstock was collected from Makassar Strait Sea. Larvae from the two sources of broodstock were reared in the conical tanks with density of 50 zoeas per liter. After reaching megalopa phase, then, the larvae were transfered into concrete tank completed with shelter and it were reared until crab phase. Crab phase was reared for 18 days, then these seed were released into the brackishwater pond for further domestication step. Larva from zoea to crab were fed with rotifer, artemia, fresh small shrimp and diet. The result showed that domesticated broodstock produced higher survival rate (P<0.05) larvae, faster larval stage changes (P<0.01) and uniform larval growth compared to the larvae from wild broodstock. It is clearly shown, the use of domesticated broodstock of blue swimming crab produced better larva quality and quantity than the wild broodstoock crab.Keywords : blue swimming crab, larvae, domestication, broodstock selection, faster growth
Habitat Preferences of Blue Swimming Crab (Portunus pelagicus) Species Complex Asphama, Andi Ivo; Amir, Faisal; Malina, Asmi Citra; Fujaya, Yushinta
Aquacultura Indonesiana Vol 16, No 1 (2015): Volume 16 Issue 1 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.69 KB) | DOI: 10.21534/ai.v16i1.10

Abstract

Blue swimming crabs (Portunus pelagicus) from Makassar Strait have some variations in color and pattern of spots on the carapace. The purpose of this study was to analyze the habitat preferences blue swimmer crab species complex from Macassart Straits.  Crabs were grouping based on the difference of color and pattern of spots on the crab carapace to identify the variety of crabs.  Habitat was observed salinity, depth, and substrate type. The results of PCA analysis showed a tendency of each population occupies a different base substrates (P<0.01). Substrate type also correlated with depth and salinity. The conclusion of this study is the difference in the color and pattern on the carapace thought to be different species or varieties and they occupy different habitats.  Preferences of the different habitats for different population groups are very important information for the development of blue swimming crab aquaculture in the future. Keywords: Crab, Portunus pelagicus, morphometric, habitat, aquaculture