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Pengaruh Mikroba Pelarut Fosfat dan Pupuk P terhadap P Tersedia, Aktivitas Fosfatase, P Tanaman dan Hasil Padi Gogo (Oryza sativa. L.) pada Ultisol Fitriatin, Betty Natalie; Yuniarti, Anny; Mulyani, Oviyanti; Fauziah2, Feni Siti; Tiara, Mohamad Dion
Agrikultura Vol 20, No 3 (2009)
Publisher : Agrikultura

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Abstract

Mikroba pelarut fosfat (MPF) berperan penting dalam mobilisasi fosfor tanah dan selanjutnya memfasilitasi penyerapannya oleh akar tanaman. Penelitian rumah kaca telah dilakukan untuk mengetahui pengaruh mikroba pelarut fosfat dan pupuk P terhadap P tersedia, aktivitas fosfatase, populasi mikroba pelarut fosfat, dan konsentrasi P tanaman serta hasil tanaman padi gogo (Oryza sativa L.) yang ditanam pada Ultisols Jatinangor. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok pola faktorial yang terdiri atas dua faktor yaitu jenis inokulan MPF dan dosis pupuk fosfat. Hasil penelitian menunjukkan bahwa tidak terdapat interaksi antara isolat mikroba pelarut fosfat dengan dosis pupuk P terhadap semua parameter penelitian. Namun inokulasi campuran Pseudomonas sp. dan Penicillium sp. mampu meningkatkan aktivitas fosfatase,  konsentrasi P tajuk hingga 19,23 % dan bobot gabah kering giling (GKG) padi gogo hingga 29,03 %. Aplikasi 75 kg P2O5 ha-1 mampu meningkatkan P tersedia hingga 20,66 % dan bobot GKG  hingga 15,23 % dibandingkan kontrol.
Populasi Mikroflora di Sekitar Rizosfer Tanaman Tomat Transgenik Miraculin dan Non-Transgenik Kultivar Moneymaker Wibawa, Rangga Jiwa; Fitriatin, Betty Natalie; Carsono, Nono
Jurnal Fakultas Pertanian Vol 1, No 4 (2014)
Publisher : Jurnal Fakultas Pertanian

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Abstract

Tanaman tomat transgenik miraculin adalah tanaman hasil rekayasa genetika yang disisipi gen miraculin dari tanaman Richadella dulcifica. Miraculin merupakan glikoprotein yang dapat memodifikasi rasa asam sehingga terasa manis di lidah. Salah satu syarat yang harus dipenuhi dalam hal pelepasan varietas tanaman hasil rekayasa genetika adalah pengujian keamanan hayati untuk mengetahui potensi keamanan lingkungan tanaman tersebut. Penelitian ini dilakukan untuk mengetahui potensi keamanan hayati tanaman tomat transgenik miraculin melalui pengujian dampak tanaman tersebut terhadap populasi mikroflora (bakteri, jamur, dan aktinomisetes) menggunakan konsep kesepadanan substansial. Pengamatan populasi mikroflora dilakukan dengan metode total plate count. Pengamatan dilakukan pada saat sebelum tanam, masa vegetatif maksimum, dan setelah panen. Hasil penelitian menunjukkan bahwa populasi mikroflora di sekitar rizosfer tanaman tomat transgenik miraculin dan tanaman tomat non-transgenik kultivar Moneymaker tidak menunjukkan perbedaan yang nyata menurut analisis statistik student t-test pada taraf kepercayaan 95%. Tanaman tomat transgenik miraculin tidak memiliki dampak yang berbeda dengan tanaman tomat non-transgenik kultivar Moneymaker terhadap populasi mikroflora. Kata kunci : mikroflora, miraculin, pengujian keamanan hayati, tomat transgenik
EFEK STERILISASI DAN KOMPOSISI MEDIA INOKULAN KONSORSIUM MIKORIZA ARBUSKULA (MA) DAN MYCORRHIZAL HELPER BACTERIA (MHB) TERHADAP JUMLAH SPORA MA, POPULASI MHB, DAN NISBAH PUPUS AKAR SORGUM (Sorghum bicolor) kartika, yeti; Nurbaity, Anne; Fitriatin, Betty Natalie; Sofyan, Emma Trinurani
Jurnal Fakultas Pertanian Vol 1, No 4 (2014)
Publisher : Jurnal Fakultas Pertanian

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Abstract

Sterilisasi dan komposisi media merupakan faktor penentu dalam menghasilkan inokulan mikoriza yang berkualitas. Penelitian ini bertujuan untuk mendapatkan kualitas inokulan konsorsium FMA dan MHB yang terbaik dengan merekayasa media tanam dan metode sterilisasi. Percobaan telah dilakukan di rumah kaca menggunakan Rancangan Acak Kelompok (RAK) pola faktorial yang terdiri atas dua faktor perlakuan. Faktor pertama adalah sterilisasi media yang terdiri atas: (1) sterilisasi melalui pencucian dengan air, (2) sterilisasi menggunakan autoklaf, (3) sterilisasi dengan NaOCl, dan (4) sterilisasi dengan irradiasi gamma. Faktor kedua adalah komposisi media yang terdiri atas: (1) 100% zeolit, (2) 75% zeolit : 25% arang sekam, dan (3) 50% zeolit : 50% arang sekam. Hasil penelitian menunjukkan bahwa terdapat interaksi antara sterilisasi dan komposisi media terhadap jumlah spora MA, namun tidak berpengaruh terhadap nisbah pupus akar sorgum. Sterilisasi media secara mandiri berpengaruh terhadap populasi MHB. Perlakuan sterilisasi irradiasi gamma + komposisi media 75% zeolit : 25% arang sekam merupakan perlakuan yang cenderung lebih baik dalam meningkatkan jumlah spora MA, populasi MHB, dan nisbah pupus akar sorgum. 
Populasi Bakteri Dan Jamur Pada Rizosfer Caisim (Brassica juncea L.) Yang Ditanam Di Tanah Dikontaminasi Insektisida Organoklorin Setelah Aplikasi Konsorsia Mikroba Dan Kompos Hindersah, Reginawanti; Rachman, Wawan; Fitriatin, Betty Natalie; Nursyamsi, Dedy
Agrologia Vol 3, No 2 (2014)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v3i2.246

Abstract

Organochlorine insecticide was banned for agriculture but nowadays its residue was still found  in some agricultural soil. One of ways to lowering organochlorine residue in soil is by used of degrading-organochlorine microbial consortia accompanied with compost application. However, application of exogenous microbes might affect bacterial and fungal population in soil. The pot experiment has been set up to verify the influence of Pseudomonas mallei and Trichoderma sp. and compost on total bacteria and fungi on rhizosphere of Cai Sim (Brassica juncea L.) grown on Andisols which were contaminated with organochlorine insecticide of heptachlor, endosulfan, dieldrin and DDT. Experimental design was Factorial Randomized Block Design with three replicates. The first factor was microbial consortia of P.mallei and Trichoderma sp., and the second one was kind of compost. The result showed that effect of microbial consortia on total bacteria population was determined by kind of compost. Total bacterial population in rhizosphere of Cai Sim grown with cow manure compost and microbial consortia was more increased.  However, microbial consortia as well as any kind of compost did not influence total fungal population in Cai Sim rhizosphere. In this experiment, total bacterial and fungal population in rhizosphere reached 109 cfu g-1 and  104 cfu g-1 respectively, indicating that major microbial population in rhizosphere was  in the normal range.
Respons tanaman jagung (Zea mays L.) akibat pemberian pupuk fosfat dan waktu aplikasi pupuk hayati mikroba pelarut fosfat pada Ultisols Jatinangor Wahyudin, Agus; Fitriatin, Betty Natalie; Wicaksono, Fiky Yulianto; Ruminta, Ruminta; Aristiyo, Muhamad
Kultivasi Vol 16, No 1 (2017)
Publisher : Fakultas Pertanian UNPAD

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Abstract

ABSTRAKJagung merupakan salah satu komoditas tanaman pangan yang mempunyai peranan strategis dalam pembangunan pertanian dan perekonomian Indonesia karena memiliki potensi dalam kebutuhan pangan, pakan, bahan baku industri, dan kerajinan tangan. Penelitian ini bertujuan untuk mengetahui pengaruh jenis pupuk fosfat dan waktu pengaplikasian pupuk hayati mikroba pelarut fosfat (MPF) terhadap pertumbuhan dan hasil tanaman jagung pada Ultisols Jatinangor. Percobaan dilaksanakan di Kebun Percobaan Ciparanje Fakultas Pertanian Universitas Padjadjaran, Jatinangor, Kabupaten Sumedang, Jawa Barat dengan ketinggian tempat +_ 750 meter di atas permukaan laut dan ordo tanah Ultisol, curah hujan rata-rata termasuk tipe C3 menurut Oldeman, dan temperatur udara berkisar antara 22,00 – 23,66 C. Percobaan dilakukan dari bulan Mei sampai Agustus 2014. Metode percobaan yang digunakan adalah Rancangan Acak Kelompok yang terdiri dari 9 perlakuan dan diulang tiga kali. Perlakuan yang diujicoba adalah sebagai berikut: control (tanpa pupuk P dan pupuk hayati MPF); pupuk SP-36 dan batuan fosfat masing-masig dengan dosis anjuran 100% tanpa pengaplikasian pupuk hayati; pupuk SP-36 dan batuan fosfat masing-masing dengan dosis 50% dari anjuran dan dikombinasikan dengan pemberian pupuk hayati MPF sebanyak 1 kali pada saat sebelum tanam, 2 kali pada saat sebelum tanam dan 4 minggu setelah tanam (MST), dan 3 kali pada saat sebelum tanam, waktu pengaplikasian pupuk hayati mikroba pelarut fosfat memberikan pengaruh terhadap panjang tongkol. Perlakuan jenis pupuk SP-36 dengan dosis 50% dari anjran dan waktu pengalikasian pupuk hayati MPF sebanyak 1 kali pada waktu sebelum tanam terbukti memberikan pengaruh yang cenderung lebih baik dan efisien terhadap hasil dari tanaman jagung.Kata kunci : jgung, pupuk fosfat, dan mikroba pelarut fosfat.      
Azotobacter chroococcum Dan Pembenah Tanah Untuk Menurunkan Serapan Kadmium Oleh Tanaman Padi (Oryza sativa L.) Hindersah, Reginawanti; Nurfitriana, Nofalia; Fitriatin, Betty Natalie
Agrologia Vol 6, No 1 (2017)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v6i1.176

Abstract

Heavy metal contamination in paddy field which came from industry nearby and agricultural input. Increased level of toxic heavy metal cadmium will threat rice production and quality. Bioremediation by used of rhizosphere bacteria is an easy, cheap and effective method to control toxic metal uptake. Pot experiment has been done to study the change in cadmium uptake after biofertilizer Azotobacter chroococcum inoculation and some soil ameliorant. Expirement has been carried out in factorial completely randomized block design with A. chroococcum and ameliorant as treatments.   Either Azotobacter inoculation or soil ameliorant didn’t change soil acidity, the soil still neutral. Rhizosphere of paddy received biochar, hay compost and biochar and inoculated with 108 cfu mL-1 Azotobacter was colonized by more Azotobacter compared to uninoculated one. All plant treated with Azotobacter has higher nitrogen uptake but lower cadmium uptake. This experiment suggested that biofertilizer Azotobacter has a significant role to decrease cadmium uptake by padi, an important food crop. 
Populasi Bakteri Pelarut Fosfat, P-Potensial Dan Hasil Jagung Yang Dipengaruhi Oleh Aplikasi MPF Pada Ultisols Jatinangor Fitriatin, Betty Natalie; Agustina, Mayang; Hindersah, Reginawanti
Agrologia Vol 6, No 2 (2017)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v6i2.171

Abstract

Ultisol is low fertility soil and as low available P due to P fixation by Al and Fe. The phosphate solubilizing microbes (PSM) is the microbial group that enable realease fixed-P to soil solution and be absorbed by plant. This research was aimed to determine the effects of PSM biofertilizer and phosphate fertilizer dosage combination on soil potential P, total phosphate solubilizing bacteria (PSB) population and yield of maize (Zea mays L.) grown in Ultisols. This research used a Randomized Block Design that consist of nine treatments with three replications. The treatments were 100 kg ha-1 of SP-36 fertilizer, 50 kg ha-1 of PSM biofertilizer, SP-36 fertilizer (50 kg ha-1 and 100 kg ha-1) with PSM biofertilizer (25 kg ha-1, 50 kg ha-1 and 75 kg ha-1) repectively. The result showed that the combination of PSM and phosphate fertilizer dosage had significantly effect on PSB total population and maize yield, but did not not on  potential P. This experiment suggested that SP-36 fertilizer with 50 kg ha-1 dosage and PSM biofertilizer with 50 kg ha-1 dosage is the best combination.
APLIKASI KONSORSIUM PUPUK HAYATI TERHADAP POPULASI BAKTERI PELARUT FOSFAT DAN BOBOT KERING PADI (Oryza sativa L.) PADA BEBERAPA TINGKAT SALINITAS Puspafirdausi, Fidya A.; Sofyan, Emma Trinurani; Fitriatin, Betty Natalie
Jurnal Agroekoteknologi Vol 9, No 1 (2017)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

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Abstract

Salinization of soil in coastal area is a serious problem and is increasing steadly. Application biofertilizer consortium is able to improve productivity of rice crops (Oryza sativa L.) grown in saline soils. Biofertilizer supplies nutrients and phytohormones that are beneficial for plant. The aim of this research was to find combination effects of biofertilizer consortium on population of phosphate solubilizing bacteria and dry weight of paddy grown in several level of salinity. This research was conducted from December 2016 to February 2017 at greenhouse of Faculty of Agriculture, Universitas Padjadjaran with altitude 752 meters above sea levels. This research used randomized block design single factor experiment which consist of eight combinations of biofertilizer (Azotobacter sp., Azospirillum sp., endophytic bacteria, PSB, and AMF) and salinity with four replications: A (non saline 0 mmhos cm-1), B (non saline of 0 mmhos cm-1 + biofertilizer), C (salinity of 2 mmhos cm-1), D (salinity of 2 mmhos cm-1+ biofertilizer), E (salinity of 4 mmhos cm-1), F (salinity of 4 mmhos cm-1+ biofertilizer), G (salinity of 6 mmhos cm-1), H (salinity of 6 mmhos cm-1+ biofertilizer). The result showed that at salinity level of 2-4 mmhos cm-1 the crops still survived growing under salinity stress. Salinity treatment of 2 mmhos cm-1 + biofertilizer significantly affected popuation of phosphate solubilizing bacteria and salinity treatment of 4 mmhos cm-1 + biofertilizer significantly affected dry weight of rice.
APLIKASI KONSORSIUM PUPUK HAYATI TERHADAP POPULASI BAKTERI PELARUT FOSFAT DAN BOBOT KERING PADI (Oryza sativa L.) PADA BEBERAPA TINGKAT SALINITAS Puspafirdausi, Fidya A; Sofyan, Emma Trinurani; Fitriatin, Betty Natalie
Jurnal Agroekoteknologi Vol 9, No 1 (2017)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/j.agrtek.v9i1.5046

Abstract

Salinization of soil in coastal area is a serious problem and is increasing steadly. Application biofertilizer consortium is able to improve productivity of rice crops (Oryza sativa L.) grown in saline soils. Biofertilizer supplies nutrients and phytohormones that are beneficial for plant. The aim of this research was to find combination effects of biofertilizer consortium on population of phosphate solubilizing bacteria and dry weight of paddy grown in several level of salinity. This research was conducted from December 2016 until February 2017 at greenhouse of Faculty of Agriculture, Universitas Padjadjaran with altitude 752 meters above sea levels. This research used Randomized Completely Block Design with single factor experiment which consisted of eight combinations of biofertilizer (Azotobacter sp., Azospirillum sp., endophytic bacteria, PSB, and AMF) and salinity with four replications: A (non saline 0 mmhos cm-1), B (non saline of 0 mmhos cm-1 + biofertilizer), C (salinity of 2 mmhos cm-1), D (salinity of 2 mmhos cm-1+ biofertilizer), E (salinity of 4 mmhos cm-1), F (salinity of 4 mmhos cm-1+ biofertilizer), G (salinity of 6 mmhos cm-1), H (salinity of 6 mmhos cm-1+ biofertilizer). The result showed that at salinity level of 2-4 mmhos cm-1 the crops still survived growing under salinity stress. Salinity treatment of 2 mmhos cm-1 + biofertilizer significantly affected popuation of phosphate solubilizing bacteria and salinity treatment of 4 mmhos cm-1 + biofertilizer significantly affected dry weight of rice.Keyword: Biofertilizer, Dry weight of rice, Paddy (Oryza sativa L.), Population of phosphate solubilizing bacteria, Saline soils
Konsorsium Pupuk Hayati Dan Amelioran Organik Terhadap Nitrogen Tanah Serta Pertumbuhan Padi (Oryza sativa L.) Pada Tanah Inceptisols Amanda, Adinda Putri; Fitriatin, Betty Natalie; Sofyan, Emma Trinurani
Jurnal Agrotek Indonesia Vol 3, No 2 (2018): Jurnal Agrotek Indonesia
Publisher : Faculty of Agriculture University of Singaperbangsa Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.763 KB) | DOI: 10.33661/jai.v3i2.1373

Abstract

Biofertilizer and organic ameliorant can be used alternative material to increase sustainable soil fertility  and yield for agricultur. This study aimed to investigate the effect of application biofertilizer consortium and organic ameliorant on chemical and biological character of soil and yield of rice in Inceptisols. The experiment was conducted in experimental field of Agriculture Faculty Universitas Padjadjaran from June until October 2017. Experimental design used a randomized block design of single faktor with twelve treatments and three replications. The treatments consist of control, solid biofertilizer, liquid biofertilizer, combination solid biofertilizer with organic amelioran (straw compost, charcoal husk and cow manure), liquid biofertilizer with organic amelioran, and organic amelioran independen. The result showed that biofertilizer consortium and organic ameliorant has significant effect on N soil and growth of rice. Solid biofertilizer and straw compost was the best treatment increase N soil.In general, biofertilizer consortium and organic ameliorant gave a significant influence for soil chemical and growth of rice. Key words: Biofertilizer, organic ameliorant, N soil and growth of rice.