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Antibacterial Activity of Water lily Seed Extract Toward Diarrhea-causing Pathogenic Bacteria Fitrial, Yuspihana; Astawan, Made; Soekarto, Soewarno S; Wiryawan, Komang G; Wresdiyati, Tutik; Khairina, Rita
Jurnal Teknologi Dan Industri Pangan Vol 19, No 2 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.455 KB) | DOI: 10.6066/350

Abstract

The objectives of this study was to observe antibacterial activities of water lily seed, against diarrhea-causing pathogenic bacteria as well as lactic acid bacteria; phytochemistry components in water lily seed and to evaluate each component’s activities against pathogen bacteria. Extraction of antibacterial components in the seed was done by fractional extraction methods using solvent based on its polar level, i.e. hexane, ethyl acetate and ethanol. The activities of each extract was tested by using diarrhea-causing bacteria, Enteropathogenic Escherichia coli K.1.1 (EPEC K1.1) and S. typhimurium with agar well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were calculated with plate counting. Qualitative phytochemical tests were performed on all extracts. Fractionation was performed on extract with the largest antimicrobial activity by using thin-layer chromatography. The activities of each fraction were tested qualitatively by bio-autography method on thin layer chromatographic plates. The water lily seed had an antibacterial activity against EPEC K.1.1 and Salmonella typhimurium, especially in ethyl acetate extract. Ethanol extract had the same, yet lower activity. Ethyl acetate and ethanol extract of the seed did not show inhibition against the growth of lactic acid bacteria (Lactobacillus sp) and Bifidobacterium bifidum. The MIC and MBC values of the ethyl acetate extract on EPEC K1.1 were 0.89 (mg/mL) and 1.33 (mg/mL), respectively, while similar values of that on S. Typhimurium were 1.11 (mg/mL) and 1.33 (mg/mL¬), respectively. Phytochemistry components within ethyl acetate extract were alkaloids, flavonoids, tannins, glycosides, saponins, and triterpenoids. All fractions in the ethyl acetate extract had antimicrobial activities against EPEC K.1.1 and S. Typhimurium. These fractions were thought to inhibit the growth of the test-microbes by synergic action of each component.Key words: water lily seed, antibacterial, ethyl acetate extract
AKTIVITAS BIOLOGIS TEPUNG BIJI TERATAI PRA-MASAK SEBAGAI PRODUK PANGAN PENCEGAH DIARE Fitrial, Yuspihana; Khairina, Rita; Oktaviyanti, Ika K
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 15, No 2 (2012): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Departement of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (662.779 KB)

Abstract

Raw waterlilly seeds are known to have activity to prevent diarrhea. It has been proven through testing both in vitro and in vivo against E. coli K1.1 Enterophatogenic (EPEC), the bacteria that causes diarrhea. This study aimed to determine the biological activity of pre-cooked waterlilly seed flour as the prevention of diarrhea through in vivo testing. Results showed that mice fed a ransum substituted with waterlilly seed flour and intervented with EPEC had an increased in body weight 25% lower compared with controls, as well as a declining in fecal water content average of 2% a day after the EPEC intervention was stopped. Rationing followed by EPEC intervention did not increase the number of microbes, causing reduction in the number of E. coli, and did not cause a decrease in lactic acid bacteria (LAB). Rationing waterlilly seed flour shown to inhibit inflammation in the intestinal villi, preventing necrosis of the intestinal mucosa cell (jejenum), reduce inflammation, and prevent adhesion of E. coli in the intestinal mucosa.Key words: diarrhea, EPEC, jejunum, waterlily seeds pre-cooked flour
AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT BIJI TERATAI (Nymphaea pubescens Willd) AKIBAT PEMANASAN Fitrial, Yuspihana
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 14, No 1 (2011): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Departement of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.455 KB)

Abstract

The purposes of this study were to evaluate the in uence of heating temperature, 100 ˚C and 121 ˚C (the autoclave temperature) on antibacterial activity and stability of water lily seed ethyl acetate extract. Research was initiated with water lily seed extract using multistage maceration extraction method based on solvent polarity level. Extraction stages beginning with a solvent hexane (non polar), then with ethyl acetate (semi-polar) so that theobtained ethyl acetate extract. In ethyl acetate extracts were subjected to heating at 100 ˚C and 121 ˚C. Each extract that has been treated, tested for antibacterial activity with tested bacteria E. coli and S. Typhimurium by using agar well diffusion method. Observations on the stability of fractions contained in the ethyl acetate extract from the treatment given, performed by using TLC (Thin Layer Chromatographi) with a mobile phase of hexane and ethyl acetate (7:3). The activities of each fraction were tested quantitatively by agar well diffusion method. Based on the results of these studies showed that heating affect the antibacterial activity of ethyl acetate extracts. The higher the temperature, decreased antibacterial activity, however at a temperature of 121 ˚ C, ethyl acetate extracts still have antibacterial activity. The process of heating effect on the stability of the ethyl acetate extract fractions. The fractions that too act as an antibacterial were 5 and 6 that relatively more polar than the fraction of 7,8,9,10 and 11. The both fractions was not stable against heating temperature of 100 ˚C and 121 ˚C.Key words: antibacterial, ethyl acetate extract, fraction, temperature
POTENSI BIJI DAN EKSTRAK BIJI TERATAI (Nymphaea pubescens Willd) SEBAGAI PENCEGAH DIARE PADA TIKUS PERCOBAAN YANG DIINTERVENSI E.coli ENTEROPATOGENIK Fitrial, Yuspihana; Astawan, Made; Soekarto, Soewarno; Wiryawan, Komang; Wresdiyati, Tutik
Agritech Vol 32, No 3 (2012)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.9611

Abstract

The aims of this study were to observe phytochemical components contained in waterlily seeds and its ethyl acetate extract; and to observe their antibacterial activities on rats that were intervened with diarrhea-causing bacteria (Enterophatogenic E. coli K1.1, EPEC). Treatment was given on the Sprague Dawley male rats (weighing 140 ± 5 g) for 28 days. Male rats were fed a basal diet (grup control) or the same diet containing 18.7 g/100g of waterlily’s seed flour or the same diet containing 6 g/100 g of FOS or a basal diet with 17.8 mg/ml waterlily’s seeds extract (orally route) for 2,3 or 4 weeks. After 2 weeks treatment feedings, rats were orally infected with 0.3 ml of 106 CFU/ml E. coli in a week. Cecal contents were collected at the end of each time period.The biological activity of treatment was observed by observing body weight, daily feed intake, feed effi ciency, total microorganisms, total E. coli and total lactic acid bacteria from the cecum contents of rats and histological picture of the small intestine of rats. The results showed that water lily seed contain alkaloids, fl avonoids, steroids, glycosides, tannins, saponins, and triterpenoids, whereas ethyl acetate extract of water lily seed contain alkaloids, fl avonoids, tannins, glycosides, saponins and triterpenoid. Substitution treatment with waterlily seed in rations affect to total E. coli in the cecum, both after EPEC intervention and after the intervention stopped. While the provision of waterlily seed extracts affect to total E. coli in the cecum after the intervention stopped. The feed effi ciency in group substituted with waterlily seed was higher than the control and its extract (P <0.05). Substitution treatment of waterlily seed and the extract could prevent damage to the intestinal villi resulting from enterophatogenic E.coli attack. The results of this study indicated that both waterlily seed and its extract contain active compounds that could prevent and inhibit the growth of E. coli causing diarrhea in rats.ABSTRAKPenelitian ini bertujuan untuk mengetahui komponen Þ tokimia yang terdapat pada biji teratai dan ekstrak etil asetat bijiteratai dan mengetahui aktivitas antibakterinya terhadap E.coli penyebab diare (E.coli Enteropatogenik K1.1, EPECK1.1) pada tikus percobaan. Perlakuan diberikan pada tikus jantan jenis Sprague Dawley (berat 140 ±5 g) selama28 hari. Tikus percobaan dibagi menjadi 4 grup yaitu, grup 1, kontrol (mendapatkan ransum standar), grup 2 yangmendapat ransum yang disubstitusi tepung biji teratai (18,7 g/100 g), grup 3 yang mendapat ransum yang disubstitusiFOS (fruktooligosakarida, 6 g/100 g), dan grup 4 yang mendapat ransum standar dan ekstrak etil asetat biji teratai(17,8 mg/ml). Setelah 2 minggu perlakuan ransum, tikus percobaan diintervensi secara oral dengan 0,3 ml dari 106CFU/ml EPEC K1.1 selama 1 minggu sehingga diare. Aktivitas biologis ransum perlakuan diamati dengan mengamatibobot badan, konsumsi ransum per hari, eÞ siensi ransum, total mikroba, total E.coli dan total bakteri asam laktatdari isi sekum tikus percobaan. Hasil penelitian menunjukkan bahwa biji teratai mengandung alkaloid, ß avonoid,steroid, glikosida, tanin, saponin, dan triterpenoid, sedangkan ekstrak etil asetat biji teratai mengandung alkaloid,ß avonoid, tanin, glikosida, saponin dan triterpenoid. Substitusi biji teratai pada ransum mampu menurunkan totalE.coli isi sekum, baik setelah intervensi maupun setelah intervensi EPEC dihentikan. Sementara pemberian ekstrakbiji teratai mampu menurunkan total E.coli isi sekum setelah intervensi dihentikan. EÞ siensi ransum pada grup yangdisubstitusi biji teratai lebih tinggi dibandingkan kontrol dan ekstrak biji teratai (P<0.05). Perlakuan substitusi bijiteratai, dan pemberian ekstrak biji teratai dapat mencegah kerusakan pada vili usus halus akibat dari serangan E.colienteropatogenik. Berdasarkan penelitian ini, baik bjii teratai maupun ekstrak biji teratai mengandung senyawa aktifyang mampu mencegah dan menghambat pertumbuhan E.coli penyebab diare.Kata kunci: Biji teratai, Enterophatogenic Escherichia coli, ekstrak etil asetat, antibakteri, diare.
Karakteristik Protein dan Nitrogen Non Protein Daging Ikan Cucut Lanyam (Charcharhinus limbatus) (Characteristics of Protein and Non Protein Nitrogen in Lanyam Shark Muscle) Fitrial, Yuspihana
- Vol 1, No 1 (2011): Fish Scientiae, Vol.1 No.1 Juni 2011
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/fs.v1i1.1175

Abstract

The aim of these research were to study the characteristics of protein and non protein nitrogen contained in Lanyam shark muscle, to obtain the protein solubility curve and iso-electric point. This research begins with the analysis of protein solubility of shark muscle at several levels of pH (from 1.5-12 with intervals of 0.5),. Then, performed classification of shark muscle protein nitrogen solubility in some solvents by using the classification Osborn,  to observed  the influence of the process of washing and boiling on  non-protein nitrogen and urea in shark muscle and to study the thermal stability of muscle protein with differential scanning calorimetric study.Based on protein solubility of Lanyam muscle at pH 1.5 to 12 obtained two points which is minimum solubility at pH 4.5 and pH 9. Based on the classification Osborn, Lanyam muscle contained albumin (28.64%), globulin (13:44%), prolamin (03.29%), glutelin (33.70%). Observation of non-protein nitrogen levels indicated that the washing process was very effective to reduce non-protein nitrogen levels up to 62.34% and urea levels up to 58% . Differential Scanning Calorimetry Study of Lanyam mince showed two types of protein that has a different stability to heat and after added 2.5% NaCl formed a peak which is a fusion of both these proteins
Antibacterial Activity of Water lily Seed Extract Toward Diarrhea-causing Pathogenic Bacteria Fitrial, Yuspihana; Astawan, Made; Soekarto, Soewarno S; Wiryawan, Komang G; Wresdiyati, Tutik; Khairina, Rita
Jurnal Teknologi dan Industri Pangan Vol 19, No 2 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.455 KB)

Abstract

The objectives of this study was to observe antibacterial activities of water lily seed, against diarrhea-causing pathogenic bacteria as well as lactic acid bacteria; phytochemistry components in water lily seed and to evaluate each component?s activities against pathogen bacteria. Extraction of antibacterial components in the seed was done by fractional extraction methods using solvent based on its polar level, i.e. hexane, ethyl acetate and ethanol. The activities of each extract was tested by using diarrhea-causing bacteria, Enteropathogenic Escherichia coli K.1.1 (EPEC K1.1) and S. typhimurium with agar well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were calculated with plate counting. Qualitative phytochemical tests were performed on all extracts. Fractionation was performed on extract with the largest antimicrobial activity by using thin-layer chromatography. The activities of each fraction were tested qualitatively by bio-autography method on thin layer chromatographic plates. The water lily seed had an antibacterial activity against EPEC K.1.1 and Salmonella typhimurium, especially in ethyl acetate extract. Ethanol extract had the same, yet lower activity. Ethyl acetate and ethanol extract of the seed did not show inhibition against the growth of lactic acid bacteria (Lactobacillus sp) and Bifidobacterium bifidum. The MIC and MBC values of the ethyl acetate extract on EPEC K1.1 were 0.89 (mg/mL) and 1.33 (mg/mL), respectively, while similar values of that on S. Typhimurium were 1.11 (mg/mL) and 1.33 (mg/mL¬), respectively. Phytochemistry components within ethyl acetate extract were alkaloids, flavonoids, tannins, glycosides, saponins, and triterpenoids. All fractions in the ethyl acetate extract had antimicrobial activities against EPEC K.1.1 and S. Typhimurium. These fractions were thought to inhibit the growth of the test-microbes by synergic action of each component.Key words: water lily seed, antibacterial, ethyl acetate extract
AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT BIJI TERATAI (Nymphaea pubescens Willd) AKIBAT PEMANASAN Fitrial, Yuspihana
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 14, No 1 (2011): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.455 KB) | DOI: 10.17844/jphpi.v14i1.3427

Abstract

The purposes of this study were to evaluate the in? uence of heating temperature, 100 ?C and 121 ?C (the autoclave temperature) on antibacterial activity and stability of water lily seed ethyl acetate extract. Research was initiated with water lily seed extract using multistage maceration extraction method based on solvent polarity level. Extraction stages beginning with a solvent hexane (non polar), then with ethyl acetate (semi-polar) so that theobtained ethyl acetate extract. In ethyl acetate extracts were subjected to heating at 100 ?C and 121 ?C. Each extract that has been treated, tested for antibacterial activity with tested bacteria E. coli and S. Typhimurium by using agar well diffusion method. Observations on the stability of fractions contained in the ethyl acetate extract from the treatment given, performed by using TLC (Thin Layer Chromatographi) with a mobile phase of hexane and ethyl acetate (7:3). The activities of each fraction were tested quantitatively by agar well diffusion method. Based on the results of these studies showed that heating affect the antibacterial activity of ethyl acetate extracts. The higher the temperature, decreased antibacterial activity, however at a temperature of 121 ? C, ethyl acetate extracts still have antibacterial activity. The process of heating effect on the stability of the ethyl acetate extract fractions. The fractions that too act as an antibacterial were 5 and 6 that relatively more polar than the fraction of 7,8,9,10 and 11. The both fractions was not stable against heating temperature of 100 ?C and 121 ?C.Key words: antibacterial, ethyl acetate extract, fraction, temperature
KUALITAS TERASI UDANG DENGAN SUPLEMENTASI PEDIOCOCCUS HALOPHILUS (FNCC-0033 Soetikno, Nooryantini; Fitrial, Yuspihana; Khairina, Rita
- Vol 1, No 1 (2011): Fish Scientiae, Vol.1 No.1 Juni 2011
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/fs.v1i1.1176

Abstract

Terasi is condiment of formed solid, its flavour typically result of shrimp fermentation or mix of them with salt or other additional substance. The aim of this research is to know  influence of the supplementation P. halophilus (FNCC-0033) isolate, to time of fermentation and quality of terasi shrimp. This research by complecated Randomized Design with 3 repetition’s. The treatment given are supplementation P. halophilus 2,5 x 104 CFU/g (A), P. halophilus 5,0  x 104 CFU/g (B) and processing terasi without addition P. halophilus (FNCC-0033) as control (treatmen O). Measure of chemical parameter total N, water content, TVB and pH, the microbiologis parameter are total microbe and total LAB, and parameter organoleptic are colour, odour and texture. The research conducting days fermentation by each every 7 days during 28 days fermentation. Based on TVB value  total microbe and total lactid asid bacteria show that had been formed at 21th  days fermentation.The result of analysis of varians showed differenct betwen observed day fermentation. The conclusion of this research showed processing terasi by supplementation of  P. halophilus have similarity wich spontanious fermentations. The total microbe are supplementation to terasi able resulted of more fermentation time is quicker than spontanious fermentation.
Diversifikasi Produk Pengolahan Ikan pada Kelompok Ibu Rumah Tangga Pengusaha Karamba Ikan Banyu Hirang, Desa Bangkal, Kecamatan Cempaka, Kota Banjarbaru, Kalimantan Selatan Soetikno, Nooryantini; Fitrial, Yuspihana; Khotimah, Iin Khusnul
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 3 No 2 (2018): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : ?Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (714.177 KB) | DOI: 10.33084/pengabdianmu.v3i2.385

Abstract

Fishery product diversification activities can be used to overcome problems that are often experienced by fish cage entrepreneurs. If there is an excess harvest of fresh fish or the yield is forced to do due to sudden changes in water conditions (such as water pollution, excess capacity of the buyer, the death of fresh fish), the problem has not resolved so far. The purpose of this activity is to provide knowledge and skills for housewife of “karamba” businesspeople in the form of making presto of soft thorn carp and nugget of tilapia in Bangkal Village, Cempaka District, Banjarbaru City, South of Kalimantan. The method used in this activity is the survey, demonstration, and evaluation of the implementation of activities. The results of this activity were in the form of nuggets of tilapia and presto of soft thorn carp produced by housewives of businesspeople from Banyu Hirang Village in Bangkal Village as participants. In addition to its delicious taste, has a high protein nutritional value, these processed products can also sell as a source of household income.
Antibacterial Activity of Melanin from Cuttlefish and Squid Ink Fitrial, Yuspihana; Khotimah, Iin Khusnul
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20, No 2 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.871 KB) | DOI: 10.17844/jphpi.v20i2.17907

Abstract

Marine environment comprises of many organism which are known to posses bioactive compound as a common means of self-defense or for the protection of eggs and embryos. Class Cephalopods (such as squidand cuttlefish) are notable for their defences, such as jetting escape movements, changes in colouration, toxic venom and inking.This study aims to compare the antibacterial activity of melanin from cuttlefish ink (Sepia sp.) with squid ink (Loligo sp.) against E. coli. Extraction and purification studies were carried out on Sepia and Loligo melanin using a hydrochloric acid 0,5M treatment under mechanical.The melanins were obtained and further evaluated their activity by direct contact methods between melanin and E. coli in nutrient broth.Total microbes was counted by total plate count.Both inks also was tested their activity against E. coli. The results showed that melanin from cuttlefish and squid inks had inhibitory activity at concentrations of 10 mg / ml and 20 mg / mL, respectively reaching 99.99% against E. coli.The inks of both Cephalopods at the same concentration as melanin, did not show any inhibitory activity against E. coli.  The melanin of Sepia sp. have a higher antibacterial activity than the melanin of Loligo sp.