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ISOLASI DAN SELEKSI BAKTERI PENDEGRADASI PARAQUAT DARI TANAH PERTANIAN DI KAMPAR RIAU

Jurnal Teknobiologi Vol 1, No 2 (2010)
Publisher : Jurnal Teknobiologi

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Abstract

Paraquat is an active material for many kind of herbicide. Paraquat is toxic to every organism,because it can release free radical which react with oxygen to form toxic superoxide. Paraquat’sadsorbsion in soil will increase its persistence as the formation of stabil structure is increase anddegradable.A study on isolation and selection paraquat degrader bacteria from agricultural soilkampar Riau. Bacteria were isolated by using N-free media that was enriched with paraquat (10ppm), then characterized by macroscopic and microscopic method.Growth test was carry out inN-free with paraquat concentration was 20 ppm and 40 ppm which purpose to perceived bacteriagrowth base on time to generate and growth curve. Result of this study obtained 14 isolatedwhich able to growth on N-free medium with 10 ppm paraquat concentration. One isolate iscoccus, Gram positive and 13 isolate rod-shaped, Gram-negative. J311, K212 and K411 wasisolate that tested of growth cause have OD value that include on high criteria based on mediantest. The fastes time to generate with 20 ppm and 40 ppm concentration was belong to isolateK.212 that was 12,99 h and 19,76 h. The longest time to generate for all concentration findableon K411 that was 14,48 h and 24,39 h. The highest growth for all concentration belong to K212that was 0,53 h-1 and 0,035 h-1 and the lowest findable on K411that was K.411 that was 0,048 h-1and 0,03 h-1.Keywords : paraquat, biodegradation, bacteria

APLIKASI MIKROORGANISME LIGNOSELULOLITIK INDIGENUS ASAL TANAH GAMBUT RIAU DALAM PEMBUATAN KOMPOS DARI LIMBAH TANDAN KOSONG KELAPA SAWIT (Elaeis guineensis Jacq.)

Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Palm oil industries usually produce side result such as empty fruit bunch (EFB) wastes. This waste can be employed as a substrate for making compost. Naturally, EFB takes a long time to produce standardized compost as its contains lignin and cellulose components. The purpose of this research was to analyze the ability of indigenous lignocellulolytic microorganisms isolated from peat soil in Riau as the bioactivator. The selected isolates consisting of 4 bacteria (BB_S27, BB_HP42, BB_HP41 and BB_K20) and 2 fungi (LIJ1 and L1J2) were subcultured on Nutrient Broth and Potato Dextrose Broth. Starters were then prepared from a combination of those isolates resulting three types of starter, namely starter I (4 isolates bacteria), starter II (4 isolates bacteria and 1 isolates fungi), starter III (4 isolates bacteria and 2 isolates fungi). As positive controls, starter IV (EM4) and starter V (bioactivator made in USA) were utilized. Starters were prepared by fermentation process during 7 days using seedling media. Composting was done by the windrow composting system utilizing 500 kg EFB as a substrate and inoculated by 50 liters of the starters at the 1st and 7th incubation time. During 35 days composting process, the substrate was enriched by water every 2 days. The best quality compost was produced by K3 treatment (a combination of 4 bacteria) as its characters almost in line with the National Quality Standard (ISO) such as N 1.57%, C/N ratio 23.89, P 0.38%, K 1.61%, blackish brown color, soil smell, unraveled texture, and the highest cell number of bacteria, fungi, and celullolytic microorganisms were with value 1.38x1012 CFU/g, 2.08x107 CFU/g dan 1.35x108 CFU/g, respectively.

APLIKASI MIKROBA LIGNOSELULOLITIK INDIGENUS ASAL TANAH GAMBUT RIAU DALAM PEMBUATAN KOMPOS DARI CAMPURAN TANDAN KOSONG DAN LIMBAH CAIR PABRIK KELAPA SAWIT (Elaeis guineensis Jacq.)

Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Activity of palm oil industries usually has side result such as empty fruit bunch (EFB) and palm oil mill effluent (POME) wastes. Those wastes still contain organic material that can be utilized as a substrate for compost production. Because of lignin and cellulose components, composting of EFB takes about 60 days to obtain standardized composts. It is known that composting process can be shorterned by adding bioactivators. The purpose of this research was to analyze the ability of indigenous lignocellulolytic microbes isolated from peat soil in Riau as bioactivator in composting of EFB enriched by POME. The selected isolates consisting of 4 bacteria (BB_S27, BB_HP42, BB_HP41 and BB_K20) and 2 fungi (LIJ1 and L1J2) were subcultured on Nutrient Broth and Potato Dextrose Broth. Starters were then made from a combination of the isolates and fermented during 7 days using seedling media. The compost treatments included negative control (seedling media without isolates), positive control by using effective microorganisms (EM) and bioactivator made in USA, and 4 combinations of those isolates resulting 7 treatments. Composting was done by the windrow composting system utilizing 400 kg EFB as a substrate and inoculated by 50 liters of the starters at the 1st and 14th incubation time. During 35 days composting process, the substrate was enriched by 20 liters POME every 2 days. The best quality compost was produced by treatment K3 (a combination of 4 bacteria and 2 fungi) as its characters almost in line with the National Quality Standard (ISO) such as N 2.22%, C/N ratio 14.5, P 0.760%, K 3.44%, blackish brown color, smell like soil and unraveled texture. The highest celullolytic and ligninolytic microbes cell number were also found in compost treated by K3 with value 8.8 x 108 CFU/g and 1.2 X 109 CFU/g, respectively.

SELEKSI ISOLAT JAMUR DALAM MENGHASILKAN HORMON IAA (Indole Acetic Acid) ASAL TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR

Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Indole Acetic Acid (IAA) is one of group of auxin phytohormones and plays a role to improve plant growth. Beside plants, fungi are also known to be able to produce IAA.This study aimed to select the ability of fungal isolates collection of Microbiology Laboratory, Faculty of Math and Natural Sciences University of Riau in IAA production. Fungal isolates were cultured in medium PDB (Potato Dextrosa Broth)enriched by tryptophan as a precursor of IAA. Indole acetic acid produced by fungi was detected by adding Salkowski reagent and determined quantitatively by the use of colorimetric method. The results showed that 47 fungal isolates were able to produce IAA. The highest IAA production was revealed by isolate RPL4-14 (646,75±0,35 ppm) and the lowest was shown by isolate RPL2-x (4,00±3,53 ppm) when they were culturedin PDB medium enriched by tryptophan. The fungal collection did not only produce IAA in the medium containing tryptophan, but also in the medium without tryptophan as shown by isolate RPL3-10, which has IAA concentration i.e 76,50±0,00 ppm and71,00±0,70 ppm, respectively. Isolates which produced the highest IAA concentration are identified as Penicillium sp.

SELEKSI KEMAMPUAN BAKTERI PELARUT FOSFAT ASAL BUKIT BATU-RIAU DALAM MENGHASILKAN ASAM SIANIDA

Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Phosphate solubilizing bacteria (PSB) is beneficial bacteria which are not only capablein solubilizing organic phosphate into inorganic phosphate, but also producing cyanideacid (HCN). HCN plays a role in controling weed growth. The objective of this researchwas to screen the potency of indigenous PSB isolated from peat soil in producting HCN.The collections of PSB were subcultured in King’B broth. The ability of 152 isolates onHCN production were tested qualitatively using filter paper soaked with picric acid andNa 2 CO 3 . The result indicated that as many as 26 isolates (17.1%) were able to releasevarious levels of HCN production based on the appearing of brown, dark brown, orreddish brown colour on the filter paper.

Seleksi Potensi Bakteri Probiotik Indigenus Riau sebagai Agen Biopreservatif Bahan Pangan

Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

The use of synthetic preservatives is not safe for consumption and very dangerous for health.Lactic acid bacteria (LAB) is a probiotic bacterium that can produce bacteriocins asbiopreservatives agents. Bacteriocins have been widely applied as a food preservative, but itscommercial availability is still low and very expensive. This study was conducted to test the abilityof 23 isolates of local probiotic bacteria in Riau to produce bacteriocins. LAB total population wascalculated by Total Plate Count (TPC) method. LAB inhibition test against indicator bacteriaStaphylococcus aureus FNCC 0047 was conducted using well diffusion method. Bacteriocinsantimicrobial confirmatory test was performed at 100OC for 15 minutes, 30 minutes, and 60minutes. The result showed that the highest LAB population was PSG4 (22.45 x 106 cfu/ml) andthe lowest was SLK2 (4.75 x 106 cfu/ml). The greatest inhibitory activity against indicator bacteriaS. aureus FNCC 0047 was found in PSG2 (15.1 mm) and the smallest was in SLK2 (10.75 mm)with the activity of bacteriocins 2575 mm2/ml and 792.6 mm2/ml respectively. The bacteriocinsconfirmatory test showed clear zone of isolates JN5, PSG2, and SLK2 with the largest inhibitionzone was during heating for 30 minutes and 60 minutes (19.2 mm, 19.5 mm, and 16.8 mm) whilethe smallest was for 60 minutes and 15 minutes (12.8 mm, 15.6mm, and 14.7 mm).Keywords: Probiotic bacteria, biopreservatives, bacteriocins, Staphylococcus aureus FNCC 0047.

FORMULASI PUPUK HAYATI SERBUK MENGGUNAKAN BAKTERI PELARUT FOSFAT INDIGENUS ASAL TANAH GAMBUT RIAU DALAM BERBAGAI BAHAN PEMBAWA

Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Peat soil has been known for its low content in phosphor (P). On the other hand, P solubilizing process is naturally slow. Inoculation of phosphate solubilizing bacteria (PSB) could increase this process. Therefore, the exploration of bacteria with such potential for use as biofertilizer agents is needed. Quality of biofertilizer depends on the viability and amount of bacteria contained, it is also influenced by type of carrier material. This research was aimed to find the appropriate carrier materials in production of biofertilizer which were contained PSB agent and to determine the quality of biofertilizer produced at a certain storage time. As many as 4 selected PSB isolates (BB_UB6, BB_K9, BB_K2, and BB_HS13) were used to produce 3 starter combinations. The starters were prepared by growing the isolates in Pikovskaya’s media. Biofertilizer was produced by inoculating each starter into the peat and wood charcoal as carrier material and was fermented for 4 days. Biofertilizer quality was determined by calculating the PSB cells number during 0, 30, 60, and 90 days storage time and the degree of biofertilizer acidity. The cell numbers of starter I, II, and III ranged from 8.2.1010-2.9.1011 CFU/g, 5.3.1010-2.9.1011 CFU/g, and 8.0.1010-2.9.1011 CFU/g, respectively in a period of storage 0-90 days. The highest cell number at the end of storage was found from biofertilizer produced by using peat, as carrier material which was kept at 40C (1,3.1011 CFU/g, starter I), (8.6.1010 CFU/g, starter II), and (9.6.1010 CFU/g, starter III). Biofertilizer acidity was relatively neutral, 6.38. Based on the results obtained, peat was revealed as a good carrier material and the quality of biofertilizers which were produced was still good.

Seleksi Isolat Aktinomisetes Asal Tanah Gambut Desa Rimbo Panjang Kabupaten Kampar Dalam Menghasilkan Hormon IAA (Indole Acetic Acid)

Jurnal Riau Biologia Vol 2, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Hormon IAA (Indole Acetic Acid) termasuk fitohormon golongan auksin yang berperan sebagai zat pemacu pertumbuhan tanaman. Selain tumbuhan, mikroba juga diketahui mampu menghasilkan IAA. Penelitian ini bertujuan untuk menyeleksi kemampuan koleksi isolat aktinomisetes Laboratorium Mikrobiologi FMIPA UR dalam menghasilkan IAA. Uji produksi IAA oleh aktinomisetes dilakukan dengan penambahan reagen Salkowski menggunakan metode kolorimetri dalam medium SCB (Starch Casein Broth) yang diperkaya dengan triptofan sebagai prekursor dan tanpa triptofan. Hasil penelitan ini diperoleh sebanyak 50 dari 85 isolat aktinomisetes yang memiliki kemampuan dalam menghasilkan hormon IAA. Produksi IAA tertinggi dihasilkan oleh RB5S78 sebesar 35,97 ppm dan konsentrasi terendah dihasilkan oleh RB4S67 sebesar 0,24 ppm dalam medium SCB yang diperkaya triptofan. Pada medium tanpa triptofan hasil tertinggi diperoleh isolat aktinomisetes RB5S78 dengan konsentrasi sebesar 15,28 ppm dan konsentrasi terendah dihasilkan oleh RB4S65 sebesar 0,16 ppm. Isolat aktinomisetes yang menghasilkan IAA dengan kriteria tinggi yang diperoleh termasuk dalam genus Streptomyces dan Rhodococcus.

FORMULASI BIOFERTILIZER CAIR MENGGUNAKAN BAKTERI PELARUT FOSFAT INDIGENUS ASAL TANAH GAMBUT RIAU

Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Inefficient of P-uptake by plants during the application of chemical phosphorus (P) fertilizer results P-leaching to the aquatic environment. Therefore, the use of phosphate solubilizing bacteria (PSB) to enhance solubilization of P is necessary. The aims of this study were to find the best formulation in producing liquid biofertilizer which contained PSB and to analyze the storage time of liquid biofertilizer which were produced. As many as 4 selected PSB isolates (BB_UB6, BB_K9, BB_K2 and BB_HS13) were used to produce 3 combination starters. Liquid biofertilizers were produced by fermentation using three types of formulation, namely Pikovskaya's medium, coconut water enriched with 2% molasses, and tofu waste water. The quality of liquid biofertilizer was determined by calculating the PSB cells number during 0, 30, and 60 days of storage time and by measuring the liquid biofertilizer acidity. Liquid biofertilizers produced were kept at room and refrigerator temperature. The results showed that the PSB cell numbers of starter I, II and III were higher in liquid biofertilizer that was formulated with coconut water which containing 2% of molasses until 60 days of storage time. The cell numbers of PSB ranged from 7,0×1010 - 2,82×1011 CFU/ml. In general, the PSB cell number was relatively stable when liquid biofertilizer was kept at room temperature. Based on this results, it can be concluded that the best formulation to produce liquid biofertilizer was the coconut water enriched with 2% molasses.

ISOLASI DAN KERAGAMAN BAKTERI UREOLITIK LOKAL RIAU YANG BERPOTENSI SEBAGAI CAMPURAN BETON

Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

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Abstract

Abstrak Bakteri ureolitik merupakan mikroorganisme yang memiliki kemampuan untuk menghasilkan urease yang dapat mengendapkan kalsium karbonat (kalsit). Bakteri ini berpotensi sebagai agen bioremediasi logam berat dan sebagai bahan konstruksi beton. Penelitian ini bertujuan untuk mengisolasi, mengkarakterisasi, dan menguji presipitasi kalsit bakteri ureolitik dari tanah lokal Riau. Hasil penelitian memperoleh 30 isolat bakteri yang berhasil diisolasi dari tanah tempat pembuangan akhir, dan setelah dilakukan pewarnaan Gram, 77% isolat merupakan bakteri Gram positif dan hanya 33% merupakan bakteri Gram negatif.  Hasil uji presipitasi menunjukkan bahwa isolat- isolat dengan kode sp. 32, sp. 9, dan sp. 20 mampu membentuk kalsium karbonat berturut-turut sebesar 0,129 g, 0,126 g, dan 0,105 g, setelah diinkubasi selama 7 hari pada medium cair yang diberi penambahan urea dan kalsium. Isolat-isolat bakteri tersebut memiliki hubungan kekerabatan yang dekat, yang ditandai dengan besarnya koefisien kekerabatannya, yaitu lebih dari 70%. Dengan demikian, bakteri-bakteri yang terisolasi dan teruji dalam  membentuk kalsium karbonat asal tanah lokal Riau berasal dari sekelompok bakteri, yang berpotensi untuk dikembangkan sebagai campuran beton.  Abstract Ureolytic bacteria are microorganisms that have the ability to produce urease that precipitates calcium carbonate (calcite). This bacteria has potential as an agent for bioremediation of heavy metal and as a concrete construction material. The aim of this research is concerning about isolation, characterization, and examination on calcite precipitate of the ureolytic bacteria from Riau local soil. The result showed that 30 isolates were isolated from landfill soil, and after Gram staining, 77% of the isolates are Gram-positive and only 33% are Gram-negative. The result of precipitation examination showed that the bacterial isolates sp. 32, sp. 9, and sp. 20 precipitated 0.129 g, 0.126 g and 0.105 g of calcium carbonate, respectively, after incubation for 7 days in broth medium added with urea and calcium. The bacterial isolates have a close relationship, which is characterized by the magnitude of the coefficient of more than 70%. Therefore, the isolated and tested bacteria having the ability to form calcium carbonate from local soil Riau derived from a group of bacteria, which has a potential to be developed as a mixture of concrete. Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 5737