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Aktifitas Inhibitor Alpha-Glukosidase Bakteri Endofit PR-3 yang Diisolasi dari Tanaman Pare (momordica charantia) Pujiyanto, Sri -; Ferniah, Rejeki Siti
Bioma Vol. 12, No. 1, Tahun 2010
Publisher : Bioma

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.973 KB) | DOI: 10.14710/bioma, 12, 1, 1-5

Abstract

Some traditional medicinal plants are known to have efficacy as a medicine for diabetes. Active compoundsproduced by a plant can be derived from endophytic microbes that live in these plants. One way diabetes drugs workis to prevent digestion of complex carbohydrates into glucose so that glucose intake is reduced. Alpha-glucosidaseinhibitor is a compound that can prevent the digestion of carbohydrates, especially starch into glucose. This studyaimed to test the inhibitory activity of alpha gluosidase PR-3 isolate, an endophytic bacteria from Momordicacharantia. The results showed that the crude extract (supernatant) from PR-3 has the capability of the enzyme alphaglucosidase inhibition that is equal to 61.2% compared with positive control compound acarbose 1 mg / ml. Theresults also showed that the use of maltose as carbon source produce the highest an alpha glucosidase inhibitor(54,97%), followed by the starch (47.77%), glucose (31.97%), fructose (44.14%) and sucrose (27.7%).
Pengendalian Hayati Penyakit Hawar Daun Tanaman Kentang Dengan Agens Hayati Jamur-jamur Antagonis Isolat Lokal Purwantisari, Susiana -; Ferniah, Rejeki Siti; Raharjo, Budi -
Bioma Vol. 10, No. 2, Tahun 2008
Publisher : Bioma

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1981.199 KB) | DOI: 10.14710/bioma, 10, 2, 51-57

Abstract

Penyakit hawar daun tanaman kentang atau yang oleh petani di Kedu, Wonosobo disebut Lodoh merupakanpenyakit yang paling serius di antara penyakit dan hama yang menyerang tanaman kentang di Indonesia. Penyakitlodoh ini disebabkan oleh serangan jamur patogen ganas Phytophthora infestans yang dapat menurunkan produksikentang hingga 90% dari total produksi kentang dalam waktu yang amat singkat. Sampai saat ini kapang patogenpenyebab penyakit busuk batang dan daun tanaman kentang tersebut masih merupakan masalah krusial dan belumada fungisida yang benar-benar efektif terhadap penyakit tersebut. Penelitian ini bertujuan mengoleksi danmengidentifikasi jamur-jamur tanah isolat lokal yang bersifat antagonis terhadap patogen penyebab penyakit busukdaun dan umbi tanaman kentang. Hasil penelitian menunjukkan bahwa penyebab penyakit busuk daun dan umbitanaman kentang di daerah sentra pembibitan tanaman kentang di Kedu Temanggung Jawa Tengah adalahPhytophthora infestans. Terdapat 17 isolat jamur isolat lokal yang dapat diisolasi dari tanah di sentra pembibitantanaman kentang tersebut. Dari 17 isolat jamur ini dapat dikelompokkan menjadi 4 kelompok isolat yang berbedamorfologi koloninya. Pengamatan secara mikroskopis menunjukkan bahwa dari 4 kelompok jamur tanah tersebutadalah dari marga Trichoderma spp, Aspergillus sp, Penicillium sp dan Phytophthora infestans.
Produksi Inulinase Pichia alni DUCC-W4 pada Tepung Umbi Dahlia (Dahlia variabilis Willd) dengan Variasi Konsentrasi Ammonium Nitrat dan Waktu Inkubasi -, Wijanarka -; Ferniah, Rejeki Siti; -, Salamah -
Bioma Vol. 10, No. 2, Tahun 2008
Publisher : Bioma

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (74.151 KB) | DOI: 10.14710/bioma, 10, 2, 58-64

Abstract

Inulinase (E.C.3.2.1.7) merupakan kelompok enzim hidrolase yang mampu menghidrolisis inulin menjadifruktosa. Produksi fruktosa secara langsung dari inulin oleh enzim inulinase hanya memerlukan satu tahap reaksienzimatis dan menghasilkan 90% fruktosa sehingga lebih efisien. Optimasi perlu dilakukan untuk meningkatkanproduksi inulinase, antara lain dengan penambahan sumber nitrogen dan optimasi waktu inkubasi. Khamirmerupakan salah satu mikroba yang dapat memproduksi enzim. Salah satu khamir inulinolitik yang berhasil diisolasidari umbi dahlia yaitu Pichia alni DUCC-W4. Tujuan penelitian ini adalah mengetahui variasi konsentrasi NH4NO3dan waktu inkubasi Pichia alni DUCC-W4 dalam memproduksi inulinase pada tepung umbi dahlia. Penelitian inidilaksanakan di laboratorium mikrobiologi, Jurusan Biologi Fakultas MIPA Universitas Diponegoro. Penentuanaktivitas inulinase dilakukan dengan metode DNS. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL)faktorial dengan 2 faktor. Faktor I (P0, P1, P2, dan P3) berupa konsentrasi NH4NO3 yang berbeda yaitu 0,029mM;0,05 mM; 0,1 mM; 0,15 mM dan faktor II (H1, H2, dan H3) berupa waktu inkubasi (12 jam,18 jam, dan 24 jam).Masing – masing perlakuan diulang sebanyak 3 kali. Data yang diperoleh dianalisis dengan menggunakan metodeANOVA. Aktivitas inulinase masing – masing perlakuan pada waktu inkubasi 12 jam yaitu 0,567 U/mL, 0,407U/mL, 0,304 U/mL, 0,486 U/mL, pada waktu inkubasi18 jam yaitu 0,761 U/mL, 0,644 U/mL, 0,543 U/mL, 0,554U/mL, sedangkan waktu inkubasi 24 jam yaitu 0,564U/mL, 0,567 U/mL, 0,529U/mL, 0,612 U/mL. Berdasarkanhasil penelitian menunjukkan bahwa penambahan konsentrasi NH4NO3 pada medium produksi dan perbedaan waktuinkubasi tidak meningkatkan aktivitas inulinase Pichia alni DUCC-W4.
Characterization and Pathogenicity of Fusarium oxysporum as the Causal Agent of Fusarium Wilt in Chili (Capsicum annuum L.) FERNIAH, REJEKI SITI; DARYONO, BUDI SETIADI; KASIAMDARI, RINA SRI; PRIYATMOJO, ACHMADI
Microbiology Indonesia Vol 8, No 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.8.3.5

Abstract

Fusarium wilt is a serious disease attacking chili plants in Central Java which cause lost of chili productivity. Fusarium wilt is caused by pathogenic fungi Fusarium oxysporum, which is host specific. The objectives of this research were to characterize the pathogenic F. oxysporum as the causal agent of fusarium wilt in chili plants and to observe the virulence of the pathogen. Fungal pathogen was isolated from Tawangmangu as an endemic area of fusarium wilt in Central Java. The fungi was characterized morphologically and identified molecularly by its internal transcribed spacer regions (ITS regions). Pathogenicity test was done to observe the virulence of the pathogen. One pathogenic strain was isolated from Tawangmangu, Karanganyar and was identified  morphologically and molecularly as F. oxysporum.  
Optimasi Isolasi DNA Cabai (Capsicum annuum L.) Berdasar Perbedaan Kualitas dan Kuantitas Daun serta Teknik Penggerusan Ferniah, Rejeki Siti; Pujiyanto, Sri
Bioma Vol. 15, No.1, Tahun 2013
Publisher : Bioma

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.999 KB) | DOI: 10.14710/bioma, 15, 1, 14-19

Abstract

Complete genome of chili has not been reported.The first step to study the genome is DNA isolation, so it is necessary to optimize the protocol to get an optimum DNA. This research aimed to optimize chili DNA isolation by variate the quantity and quality of chili leaves as row material and variate the grinding technique. DNA isolation was done using commercial kit without liquid nitrogen, and analyzed by agarose gel electrophoresis. The results showed that frozen chili leaf yields more DNA than fresh leaf, 0,1 g of leaf got optimum DNA, and grinding in mortar was better than in microtube.   Key words: DNA,  isolation, Capsicum annuum
Indonesian red chilli (Capsicum annuum L.) capsaicin and its correlation with their responses to pathogenic Fusarium oxysporum Ferniah, Rejeki Siti; Pujiyanto, Sri; Kusumaningrum, Hermin Pancasakti
NICHE Journal of Tropical Biology Vol. 1, No. 2, Year 2018
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.917 KB) | DOI: 10.14710/niche.1.2.7-12

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Red chili is a commercial crop for the food industry in Indonesia. There are some categories of red chili based on their pungency. The hot chili usually has more capsaicin than the sweet chili. Some cultivars may have more resistance to pathogen infection than the others. This research aimed to analyze the disease resistance of red chili cultivars from Indonesia against pathogenic Fusariumoxysporum and the correlation with capsaicin contents. Disease resistance was examined by determination of the Disease Severity Index (DSI) 15 dpi (days post inoculation). The correlation was analyzed by the regression coefficient. The result showed that the most resistance cultivar against F. oxysporum was Branang, while Lembang-1displayed the contrary. There was not a correlation of capsaicin content with the chili resistance to F. oxysporum.
Phylogenetic of Ornamented Chilli Based on the Fruit Morphology and Molecular Marker using OPA-12 Primer Ferniah, Rejeki Siti; Kusdiyantini, Endang; Nurhayati, Nurhayati
Agric Vol 30 No 1 (2018)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.436 KB) | DOI: 10.24246/agric.2018.v30.i1.p57-62

Abstract

There are many varieties of ornamental chilli that may different from their ancestor and cause genetic variability. This research aims to determine the relationship of ornamental chillies in Indonesia based on their fruit morphology and RAPD-PCR. Morphological was analysed by description method, while molecular was analysed by RAPD-PCR using OPA 12 primer. Result showed that Katty, Naga Morich, Big Black Mama, and Yellow Primo were Capsicum chinense based on their morphology and RAPD-PCR using OPA-12 primer.
Analysis of The Open Reading Frame (ORF) 29-TrnC (GCA) Sequence to Detect Indica and Japonica Sub Species on Upland Rice of Situ Bagendit and Inbred Rice of Ciherang Istiana, Rohma; Kusumaningrum, Hermin Pancasakti; Ferniah, Rejeki Siti
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12626

Abstract

The identification and the characterization of genetic diversity of rice was the first step in the rice plant breeding program. This study aimed to detect indica or japonica sub-species on upland rice Situ Bagendit and inbred rice Ciherang using molecular markers ORF 29-TrnC (GCA) on the chloroplast genome. Rice was included to the indica sub-species if the 32 bp insertion on ORF 29-TrnC (GCA) sequence was found, on the contrary, if the deletion 32 bp on ORF 29-TrnC (GCA) was found then it was included to the japonica sub-species. DNA isolation was examined from the leaves of the rice plants, and then it tested quantitatively to determine the transparency and DNA concentration from the isolation results. PCR amplification was performed using a pair of primers CP2 and it was followed by agarose gel electrophoresis. The visualization of the DNA bands used the gel documentation. Sequencing of PCR products produced a long base 390 bp in Situ Bagendit rice and 390 bp in Ciherang rice. Analysis of the sequences showed that the insertions occurred throughout the 32 bp in Situ Bagendit rice and the insertions occurred throughout the 32 bp in Ciherang rice. The results showed that upland rice Situ Bagendit and inbred rice Ciherang were included in the indica sub-species. The knowledge of variety of genetics of rice can be used as bio-information in the plant breeding program. Further, the knowledge can be used to protect in genetic power source, the selection and the composing of superior varieties of rice which is tolerant with kinds of biotic and abiotic factor.
Polimorfisme Cabai Rawit dan Cabai Gendot dengan Penanda RAPD (Random Amplified Polymorphic DNA) Menggunakan Primer OPA-8 Purnomo, Eko; Ferniah, Rejeki Siti
Berkala Bioteknologi Volume 1 Nomor 1 April 2018
Publisher : Berkala Bioteknologi

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Abstract

comABSTRAKJenis cabai di Indonesia sangatlah beragam, beberapa di antaranya adalah cabai gendot dan rawit merah.Keberagaman cabai yang tinggi dapat dianalisis secara molekuler melalui pemetaan genetik. Tujuan penelitianini adalah untuk mengetahui keragaman genetik dari sampel cabai gendot dan cabai rawit merah melalui analisispenanda RAPD. Metode yang dilakukan meliputi isolasi DNA dengan menggunkaan Wizard Genomic DNAPurification Kit Promega dan buffer Cetylrimethylammonium bromide (CTAB), lalu sampel diamplifikasidengan primer OPA-8. Hasil pengujian dengan penanda RAPD menghasilkan 75% polimorfisme DNA di antaracabai gendot dan cabai rawit merah. Hasil analisis keragaman genetik mendapatkan nilai indeks kesamaan 0,4.
Identification and Cluster Analysis of Pitcher Plant (Nepenthes spp.) from South Sumatera Indonesia Lestari, Weni; Jumari, Jumari; Ferniah, Rejeki Siti
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.13968

Abstract

Nepenthes spp. is a typical plant of Southeast Asia especially Indonesia which has a special leaf modification called a pitcher. The largest number of Nepenthes spp. species in Indonesia is on the island of Sumatra. The purpose of this reseach was to identify and analyze cluster Nepenthes spp. from South Sumatra based on morphological characteristics. The specimens were collected from the forest of Tekorejo Village, Air Itam Village and cultivation location in Palembang city of South Sumatra. Identification of morphological characters performed on the characteristics of root, stem, leaves, and pitcher. The morphological data is used for cluster analysis using NTSYS software version 2.02. The identification results showed 9 variants of Nepenthes spp. which belong to the species N. mirabilis, N. gracilis, and N. sumatrana. Dendogram analysis results form two main clusters with a similarity value of 22%. The first cluster consists of N. mirabilis and N. sumatrana. The second cluster consists of N. gracilis. Based on the results of this study can be concluded that the species Nepenthes spp. South Sumatra is N. mirabilis, N. gracilis, and N. sumatrana. The results of this study will be dedicated to updating information about the existence of Nepenthes spp. from South Sumatra and his cluster.