Dewi Fatma
Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430

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The Effects of Triethylene Glycol Dimethacrylate (TEGDMA) on the Protein of Human Dental Pulp Cells Farida, Ratna; Fatma, Dewi; Karina, Karina; Tanaya, Tanaya S.; Pardamean, Pardamean RAS
Journal of Dentistry Indonesia Vol 16, No 3 (2009): December
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

Triethylene glycol dimethacrylate (TEGDMA) is a common component of the bonding agents and resin composites used in dentistry for restorative dentistry. However, TEGDMA could be released from composite resins following incomplete polymerization and degradation processes by salivary enzyme in the mouth. Subsequently, TEGDMA is available in saliva and diffuses toward and affects the dental pulp which contains various cells, and thus may cause severe cytotoxic effects. Objectives: To determine the total protein concentration of human dental ulp cells following exposure to TEGDMA. Materials and Methods: Dental pulp cells were isolated from the pulp of the freshly extracted teeth and cultured in DMEM for 48 h (37ºC, 5% CO2). Then, 2 mM and 4 mM, and 8 mM TEGDMA were added to these cells and incubated for 24 h. The total protein was measured by Bradford Protein Assay. Results: The total protein concentration of dental pulp cells after exposure to 4 mM, 8mM, and 12 mM TEGDMA were statistically lower (22762.27 µg/ml ± 3385.87; 20268.44 µg/ml ± 1701.14; 23706.51 µg/ml ± 3214.52; respectively) than the control group (24253.77 µg/ml ± 3072.99). Furthermore, the total protein concentration of culture medium after exposure to 4 mM, 8 mM, and 2 mM TEGDMA, were statistically higher (28635.85 µg/ml ± 2373.4; 35288.41 µg/ml ± 3469.48; 38199.79 µg/ml ± 2752.47; respectively) when compared with the controls (27073.83 µg/ml ± 2772.47). Conclusion: 2 mM, 4 mM, and 8 mM TEGDMA caused cytotoxity to human dental pulp cells chowed by decreasing the total protein of cells and increasing the total protein of the culture medium.DOI: 10.14693/jdi.v16i3.102
THE EFFECT OF OESTROGEN AND OR CALCIUM VITAMIN D3 ON THE MANDIBULAR HEIGHT OF POST OVARIECTOMIZED WISTAR RATS Henri, Henri D.; Erwin, Erwin; Jimmy, Jimmy G.; Djamal, Niniarty Z.; Fatma, Dewi; Soekanto, Sri Angky
Journal of Dentistry Indonesia Vol 10, No 1 (2003): APRIL
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

One of the major health problems in elderly women is osteoporosis post menopause. Dentists must be aware of the disease since its involvement on the jaw. Nowadays, there are a lot of researches on correlation of osteopororsis and mandible but only few concentrate on hormonal substitution therapy and/or Calcium-vitamin D3 (Ca-Vit D3). This research is to evaluate the effect of hormonal substitiution therapy and/or Ca-vit D3 on mandibular height. Forty five rats used in this research and divided into nine groups: one control group, two ovariectomized (OVX) groups, two OVX groups treated with estrogen, two OVC groups treated with Ca-vit D3, two OVC groups treated with estrogen and Ca-vit D3. All of the rats except the control group were ovariectomized as model for postmenopausal estrogen deficiency state. The treatment was done in two or four weeks. The animals were killed with cervical dislocation, the mandible were excised and soaked on Hydrogen Peroxide 10%. Then the mandibles heights on right buccal side were measured from the manduble base to the alveolar crest. It is concluded that hormonal substitution therapy and combination of the hormonal substitution therapy and Ca-vit D3 can maintain the normal mandibular height. Mandibular height of groups with therapy using Ca-vit D3 have slightly lower means compared to control group but without significant difference statistically. The best therapy is combination of hormonal substitution therapy and Ca-vit D3.
PENGARUH TERAPI SULIH HORMON ESTROGEN, PREPARAT KALSIUM DAN KOMBINASINYA PADA TULANG MANDIBULA (Studi eksperimental laboratorik pada tikus Wistar setelah ovariektomi) Djamal, Niniarty Z.; Gunawan, H. A.; Fatma, Dewi; Soekanto, Sri Angky; Gultom, Ferry; Utami, S.
Journal of Dentistry Indonesia Vol 10, No 2 (2003): AUGUST
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

The purpose of this study was to evaluate the effects of ovariectomy, the influences of estrogen replacement therapy and/or suppelment CavitD3 on mandibles calcium contents of animals models. Forty-five female rats were used in this research. Five rates were sacrificed for unoperated basal controls and the others were bilaterally ovariectomized. The ovariectomized animals were divided into 8 groups and two of these groups were only ovariectomized, the others were treated by estrogen replacement therapy. CavitD3 and combination of both per oral for 2 and 4 weeks and then were sacrificed. Mandibles calcium contents were analyzed by Ion Selective Electrode after demineralized with 10% phosphoric acid for 24 hours. Result : there were significant increased of mandibles calcium content at 2 weeks post-ovariectomy, but the results decreased at 4 weeks post-ovariectomy. Estrogen replacement therapy and treated with CavitD3 for 2 and 4 weeks or combination of both for 2 weeks increased the mandibles calcium contents of ovariectomized rats, but therapy combination with estrogen and CavitD3 for 4 weeks had a protective effect to mandibles calcium contents of ovariectomized rats.
Rat Microglia Cells: Their Culture, Isolation and Phagocytic Activity Fatma, Dewi; Nakanishi, Hiroshi
Journal of Dentistry Indonesia Vol 12, No 1 (2005): April
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

Microglia were isolated from mixed primary cell cultures of the cerebral cortex from 3day old male Wistar rats. The mechanically dissociated cells were plated in a flask at a density of 107per 300 cm2 and maintained at 37°C in a 10% Co2/90% air atmosphere. After 10-14 days in culture, floating and attached cells on the mixed primary cultured cell layer were isolated by gentle shaking of the flask for 5 min. The resulting cell suspension was transferred to plastic dishes and allowed to adhere at 37°C . To investigate the morphological change of microglia, the cells after 2 days of culture were incubated with biotinylated GSA-1-B4 (10µg/ml) at 4°C for overnight. To detect the phagocytic activity, isolated microglia were incubated with opsonized zymosan (20mgl/ml) for Ih at 37°C and with Giemsas staining solution for 30 min at room temperature. The results were about 90% of attached cells  had amoeboid and rod-shaped cell bodies with no or a few thick processes. Most of these cells became amoeboid-like cells and showed a number of vacuoles in the cytosol when cultured in the presence of IFN-ɣ+LPS. Both control and IFN-ɣ + LPS – treated cells exhibited the intense phagocytic activity against zymosan particles.