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Insecticidal Bufadienolides from The Leaves of Kalanchoe daigremontiana (Crassulaceae) Hermawan, Wawan; Maharani, Rani; Fajriah, Sofa; Hardiawan, Revan; Supratman, Unang
Jurnal ILMU DASAR Vol 11, No 2 (2010)
Publisher : Fakultas MIPA Universitas Jember

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Abstract

Kalanchoe is the biggest genera of Crassulaceae family and distributed in tropical and subtropical regions. This genera is found to be a rich source of biologically active natural products such as triterpenes, flavonoids and steroids. As a part of our continuing search for novel insecticidal compounds from Indonesian Kalanchoe plants, we examined Kalanchoe daigremontiana collected from Bandung region, West Java, Indonesia. The methanolic extract of the dried leaves of K. daigremontiana was concentrated and extracted with methylene chloride The methylene chloride extract exhibited an insecticidal activity toward silkworms. The methylene chloride extract was partitioned between n-hexane and methanol containing 10% water. The active lower layer was extracted with ethyl acetate. By using the insecticidal activity to follow the separations, the ethyl acetate fraction was separated by combination of column chromatography on Kieselgel 60 and ODS to afford two insecticidal bufadienolides 1 and 2. The structures of these compounds were elucidated based on spectroscopic analysis (UV, IR, NMR, 2D-NMR) and comparison with those related data previously reported. In this paper, the isolation, structural elucidation, and insecticidal activities against the third instar larvae of silkworm will be described. Keywords: Kalanchoe daigremontiana, Crassulaceae, bufadienolides, insecticidal activity
Toxicological Evaluation of Tetrameles nudiflora Methanolic Extract Banjarnahor, Sofna Dewita Sari; Dewijanti, Indah D; Angelina, Marissa; Megawati, Megawati; Fajriah, Sofa
Journal of Tropical Life Science Vol 5, No 1 (2015)
Publisher : Journal of Tropical Life Science

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Abstract

Exploration of Mekongga national park resulted in novel finding of anticancer property from Tetrameles nudiflora. The investigation of toxic features of T. nudiflora is vital for further studies of its pharmacological activities. Acute toxicity test was done on methanolic extracts of T. nudiflora in DDY mice. Animals were divided into 5 groups: Group 1 was given 1 ml solution of 2.5% Tween 80 in a single oral dose; Group 2, 3, 4 and 5 were orally administrated a single dose of 1, 2, 4 and 8 g/kg body weight T. nudiflora, respectively. Toxic effects of the extract were evaluated on the basis of behavioral observations in the form of locomotor activity; curiosity; defecation; urination and also animal mortality. Observations were carried out for 14 days. No significant changes on body weight, and behavioural activities were recorded. Mortality was recorded up to 22% of the male group, and 11% within the female group. The T.nudiflora extracts tested for toxicity against brine shrimp had 50% lethal concentration (LC50) values of 46.67 μg/ml. It can be concluded that methanol extracts of T. nudiflora are potential to be explored as anticancer (LC50=46.67 μg/ml). The extract is slightly toxic in male mice with Lethal Dose 50 (LD50) 12.6 g/kg body weight, and practically non toxic for female (LD50>15 g/kg body weight).Key words : Tetrameles nudiflora, brine shrimp lethality test, acute oral toxicity, LD 50.
PENAPISAN FITOKIMIA DAN UJI TOKSISITAS DARI DAUN Myristica fatua HOUTT Fajriah, Sofa; Megawati, Megawati
Chimica et Natura Acta Vol 3, No 3 (2015)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (100.947 KB) | DOI: 10.24198/cna.v3.n3.9219

Abstract

Myristica fatua merupakan salah satu tumbuhan obat asli Indonesia yang berpotensi untuk dikembangkan. Telah dilakukan penapisan fitokimia dan uji toksisitas menggunakan metode BSLT (Brine Shrimp Lethality Toxicity) terhadap ekstrak metanol, ekstrak aseton dan etanol 70%. Selanjutnya ekstrak metanol difraksinasi untuk memperoleh fraksi n-heksana, etil asetat, dan n-butanol dari daun Myristica fatua Houtt dan dilakukan uji toksisitas lebih lanjut. Hasil penapisan fitokimia menunjukkan bahwa daun M. fatua mengandung senyawa terpenoid, steroid, flavonoid, saponin, dan tanin. Potensi antikanker juga ditunjukkan terhadap ekstrak metanol dan fraksi etil asetat yang mempunyai toksisitas paling tinggi dari fraksi lainnya dengan LC50 masing-masing adalah sebesar 64,57 dan 83,75 μg/mL.
Apigenin, an Anticancer Active Compound Isolated from Macaranga gigantifolia Leaves Fajriah, Sofa; Megawati, Megawati; Darmawan, Akhmad
Journal of Tropical Life Science Vol 6, No 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.02

Abstract

Apigenin, a flavonoid compound has been isolated from the ethyl acetate fraction of methanol extract of Macaranga gigantifolia leaves. Isolation and purification of apigenin conducted using chromatographic method, and chemical structure characterized based on spectroscopic data. In vitro anticancer activity test against murine leukemia P-388 cell line showed potential activity with IC50 14.13 μg/mL.Key words: apigenin, flavones, Macaranga gigantifolia, anticancer, murine leukemia P-388 cell line.
MACARANGIN, A GERANYLATED FLAVONOID AND ANTICANCER ACTIVE COMPOUND ISOLATED FROM ETHYL ACETAT FRACTION OF Macaranga gigantifolia LEAVES Darmawan, Akhmad; Suwarso, Wahyudi P.; Kosela, Soleh; Kardono, Leonardus B.S.; Fajriah, Sofa
INDONESIAN JOURNAL OF PHARMACY Vol 26 No 1, 2015
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (705.678 KB) | DOI: 10.14499/indonesianjpharm26iss1pp52

Abstract

Macaranga known locally as mahang-mahangan has uniquely ecological function, and also became a part of traditional medicine in Indonesia. Macaranga genus also known as a sources of terpenoid and phenolic (flavonoid) compounds which have biological activity as antioxidant and anticancer (cytotoxicity).There are few phytochemical investigations have been done on M. gigantifolia species. As a part of our continuing research of isolation anticancer compound from natural product, a geranylated flavonoid compound (macarangin) has been isolated from ethyl acetate fraction of Macaranga gigantifolia leaves using chromatography methods. The isolated compund (isolat MG) was elucidated to gain the chemical structure based on spectroscopic data (LC-MS and FT-NMR). Cytotoxicity test of this compound was tested against MCF 7 cell lines, showed that macarangin has a potential activity with IC50 value 119.12μg/mL. Key words: Macarangin, geranylated  flavonoid, Macaranga gigantifolia, MCF 7 cell lines.
Insecticidal Bufadienolides from The Leaves of Kalanchoe daigremontiana (Crassulaceae) Hermawan, Wawan; Maharani, Rani; Fajriah, Sofa; Hardiawan, Revan; Supratman, Unang
Jurnal ILMU DASAR Vol 11 No 2 (2010)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Kalanchoe is the biggest genera of Crassulaceae family and distributed in tropical and subtropical regions. This genera is found to be a rich source of biologically active natural products such as triterpenes, flavonoids and steroids. As a part of our continuing search for novel insecticidal compounds from Indonesian Kalanchoe plants, we examined Kalanchoe daigremontiana collected from Bandung region, West Java, Indonesia. The methanolic extract of the dried leaves of K. daigremontiana was concentrated and extracted with methylene chloride The methylene chloride extract exhibited an insecticidal activity toward silkworms. The methylene chloride extract was partitioned between n-hexane and methanol containing 10% water. The active lower layer was extracted with ethyl acetate. By using the insecticidal activity to follow the separations, the ethyl acetate fraction was separated by combination of column chromatography on Kieselgel 60 and ODS to afford two insecticidal bufadienolides 1 and 2. The structures of these compounds were elucidated based on spectroscopic analysis (UV, IR, NMR, 2D-NMR) and comparison with those related data previously reported. In this paper, the isolation, structural elucidation, and insecticidal activities against the third instar larvae of silkworm will be described.
Toxicity and Lethality Evaluations of Koordersiodendron pinnatum Leaves Methanolic Extract in DDY Mice Banjarnahor, Sofna Dewita Sari; Dewijanti, Indah Dwiatmi; Angelina, Marissa; Fajriah, Sofa
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.10

Abstract

The study of toxic features of Koordersiodendron pinnatum is vital for further studies of its pharmacological activities. Acute toxicity test was done on methanolic extracts of K. pinnatum in DDY mice. Animals were grouped into five: Group 1 was given 1 mL solution of 2.5% Tween 80 in a single oral dose; the remaining groups were orally given a single dose of 2, 4, 8 and 16 g/kg of K. pinnatum, respectively. Toxic effects of the extract were evaluated on the basis of behavioral observations in the form of locomotor activity; curiosity; defecation; urination and also animal mortality. Observations were carried out for 14 days. No significant changes on body weight, and behavioural activities were recorded. Mortality was recorded up to 2% of the male group, and no mortality within the female group. The extract is practically non toxic for both male and female (LD50>15 g/kg).
ISOLATION AND IDENTIFICATION OF 9-METHOXYCANTHIN-6-0NE COMPOUND FROM Eurycoma longifolia ROOTS Fajriah, Sofa; Hanafi, Muhammad; Sumiati, Atiek; Ngadiman, Ngadiman
Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry) Vol 12, No 2 (2010)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2282.305 KB) | DOI: 10.14203/jkti.v12i2.213

Abstract

9-Methoxycanthin-6-one compound has been isolated from Pasak Bumi (E. Longifolia) roots. The isolation process using maseration, colum chromatography vacuum, and recrystallization techniques. E. Longifolia root maserated with n-hexane and methanol respectively. The methanol extract fractionated using column chromatography vacuum with gradient elution (hexane-etil acetate) to obtain 9 fractions. Fraction 5 further purification using recrystallization technique to obtain yellow crystal, identified by LC-MS and NMR spectroscopy as 9-Methoxycanthin-6-one.Keyword: E. Longifolia, isolation, spectroscopy, 9-Methoxycanthin-6-one.
AKTIVITAS ANTIOKSIDAN DAN TOKSISITAS EKSTRAK AIR DAN ETANOL DAUN BENALU (Dendrophthoe pentandra L. Miq) YANG TUMBUH PADA BERBAGAI INANG Artanti, Nina; Widayati, Retno; Fajriah, Sofa
Jurnal Kimia Terapan Indonesia Vol 11, No 1 (2009)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2843.157 KB) | DOI: 10.14203/jkti.v11i1.175

Abstract

Mistletoes are used traditionally in Indonesiaas medicinal plant, one of this as anticancer. Cancer isone of degenerative diseases could be triggered by toomuch free radicals in the body, whereas antioxidantcould reduced the risk of degenerative diseases.Mistletoes reposted content flavonoids which known have antioxidant activity. Mistletoe potency as drugmaterial should be studied sa the utilization ofmistletoe could be developed. Antioxidant activitytest using "DPPH free radical scavenger" methodand toxicity test using BSLT (Brine Shrimp LethalityTest) method had been conducted on water andethanol extracts of mistletoe Dendropthoe pentandra(L.) Miq.) that grown on various trees (star fruit,mango, cananga, duku, sour-sop, kepel, mahkotadewa, and tea). The result showed that ethanol andwater extracte-ofD, petandra on all host plants haveantioxidant activity (IC50 value between 6.4 - 51.8ug/mL). The result of toxicity test showed that ethanolextract of mistletoe on cananga, starfruit, kepel andmahkota dewa host plants have toxicity effect againstA. salina larvae with LCso value below 1000 ug/mL,nevertheless ethanol extract on others host plants andwater extract didn It give toxicity effect against A.salina larvae (LC50 > 1000 ug/mL).Key words: mistletoe, D. petandra, DPPH, antioxidant,toxicity, degenerative disea.
ANTI DIABETIC FLAVANONE COMPOUND FROM THE LEAVES OF Artocarpus communis Lotulung, Puspa D.N.; Fajriah, Sofa; Sundowo, Andini; Filaila, Euis
Indonesian Journal of Chemistry Vol 9, No 3 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.149 KB) | DOI: 10.22146/ijc.21524

Abstract

The Flavanone compound with anti diabetic activity was isolated from ethyl acetate extract of Artocarpus communis leaves using column chromatography techniques. The structure of the flavanone compound was elucidated on the basic of spectroscopic evidence and comparison to published values. This compound, 8-geranyl-4,5,7-trihydroxyflavone, showed strong anti diabetic activity on α-glucosidase inhibition assay with IC50 18.120 µg mL-1.   Keywords: Artocarpus communis, 8-geranyl-4,5,7-trihydroxyflavone, anti diabetic activity