Mokhamad Fahrudin
Bagian Embriologi, Departemen Anatomi, Histologi dan Embriologi Fakultas Kedokteran Hewan Institut Pertanian Bogor, Bogor

Published : 17 Documents
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Karakteristik dan komposisi semen kancil (Tragulus javanicus) yang dikoleksi dengan elektroejakulator

Jurnal Anatomi Indonesia Vol 1, No 1 (2006)
Publisher : Jurnal Anatomi Indonesia

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Abstract

The ejaculates were taken by electroejaculation from four apparently healthy young adultmale lessermousedeer (Tragulus javanicus). The animals were anesthetized with a combination of xylazin and ketamine followedby ether per inhalation anesthesia. The semen was white, yellowish, or creamy in color. The semen had a pH of7.63±0.22. Themean values for volumewas 19.44±6.8 μl, spermconcentration was 47.44 ± 4.9 x 106 sperm/ml,percentage of sperm motility was 36.43±1.1 %, percentage of live sperm was 53.11±3.0 %, percentage ofabnormal spermatozoa was 21.03±1.05%and percentage of intact acrosome was 52.28±2.7%, respectively.These values were relatively lowwhen compared to other domestic ruminants and suggested to be relatedwithage and sexualmaturity of the animal.The seminal plasma contained 10.2–11.5mg/100ml fructose, 22.07–24.5mg/100ml citric acid, 65mg/100ml proteins, 22.07–24.5mg/100ml sorbitol, 91.1– 94.72mg/100ml sodium, 0.1mg/100ml potassium, 12.8mg/100ml calcium, 0.8mg/100mlmagnesiumand 10.72–11.2 chloride. By SDS PAGE,eleven proteins with different molecular weight were determined in the seminal plasma. Among them, theproteins with 64.77 kDa and 71.72 kDa were particularly prominent.

Inhibitory Effect of Iodoacetate on Developmental Competence of Porcine Early Stage Embryos In Vitro

HAYATI Journal of Biosciences Vol 16, No 1 (2009): March 2009
Publisher : Bogor Agricultural University, Indonesia

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Abstract

In porcine preimplantation embryos, glucose-utilizing pathways are reported active throughout their development. The aim of this study was to test the involvement of glycolysis activity in early stage of porcine embryo development by the addition of iodoacetate, a glycolytic inhibitor, to culture medium. In vitro matured and fertilized oocytes were cultured for the first 2 days in iodoacetate at concentrations of 0, 1, or 2.5 µM. The proportion of viable embryos on day 4 decreased when Ia was added at concentration of 2.5 µM (P < 0.05). The development of cleaved embryos to the blastocyst stage on day 6 was significantly reduced by 1 µM iodoacetate and none of cleaved embryos in Ia-2.5 group develop to the blasyocyst stage (P < 0.05). More than 60% of embryos in Ia-2.5 group were arrested at the two-four-cell stage, and then arrested at the morula stage. These results indicate that glucose plays important roles in supporting the development of early stage of porcine embryos and that the inhibition of glucose metabolism may disrupt the management of energy production, leaving the embryos incompetence to develop. Key words: iodoacetate, glucose, early stage embryos, in vitro culture

Allotransplantasi Testis Mencit Muda sebagai Upaya Preservasi Gonad In Vivo

Jurnal Ilmu Pertanian Indonesia Vol 12, No 1 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Cancer disease are not detected only at adult but also at young age. One therapy for cancer diseases is chemotherapy and radiation, that give a side effect of infertility in the gonad, therefore, it is necessary to preserve the gonad. Sperm collection from adult is easy but not from the young patients.Keyword: transpalantation, testis, mencit muda, spermatogenesis

SEBARAN ANTIAGLUTININ SPERMATOZOA DALAM PLASMA YANG DIKOLEKSI DARI EPIDIDIMIS DAN EJAKULAT DOMBA THE DISTRIBUTION OF SPERM ANTIAGGLUTINI IN PLASMA COLLECTED FROM EPIDIDYMIS AND EJACULATE OF RAM

Jurnal Veteriner Vol 8, No 1 (2007)
Publisher : Jurnal Veteriner

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Abstract

Penelitian untuk mengetahui tingkat aglutinasi antarkepala spermatozoa dan sebaran antiaglutinin dalam plasma asal epididimi dan ejakulat telah dilakukan untuk mengoptimalkan pemanfaatannya dalam fertilisasi in vitro. Tingkat aglutinasi dan aktivitas antiaglutinin plasma epididimis dan ejakulat domba dihitung dengan cara menghitung jumlah aglutinasi antarkepala spermatozoa setelah diinkubasikan selama 1, 3, 5, dan 7 jam in vitro dalam media Krebs Ringer- HEPES.

STUDY ON SPERM AGGLUTINATION WITH CHARACTERIZATION OF PLASMACOLLECTED FROM EPIDIDYMIS AND EJACULATE IN RAM

Jurnal Veteriner Vol 9, No 4 (2008)
Publisher : Jurnal Veteriner

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Abstract

This study was designed to optimalize the use of epididymal or ejaculate sperm and plasma for in vitro fertilization, that sperm agglutination was found at preparation. The rate of sperm agglutination was calculated the head-to-head sperm agglutination that were incubated in Krebs Ringer-(N-(2hydroxyethyl)piperazine-N’-(2-ethenesulfonic acid) or KR-HEPES medium in 38.50C with 5% CO2 at 1, 3, 5 and 7 hours culture in vitro. The rate of head-to-head sperm agglutination were decreased with time treatments. The cauda of sperm agglutination was lower than that caput, corpus epididymal and ejaculate sperm with statistically significant (P<0.01). These result reflected that distribution of anti-agglutinin might be higher in cauda epididymal than that other areas. Number of protein were characterize with SDS-PAGE as follow 11 bands in caput epididymal, 9 bands in corpus epididymal, 2 bands in cauda epididymal and 4 bands in seminal plasma. The higher distribution of protein was found at range 25-40 kDa in epididymal plasma of ram. However, further investigation should be conducted to determine presumptive anti-agglutinin by advance method.

Optimization of Activation Methods for Mouse Oocytes Using Calcium-free CZB Medium, SrCl2, and Cytochalasin B in Vitro

Jurnal Kedokteran Maranatha Vol 8, No 2 (2009)
Publisher : Universitas Kristen Maranatha

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Abstract

Embryonic stem cells can be obtained by generating an embryo through fertilization; however, an embryo can also be generated asexually through parthenogenesis. This procedure will overcome the ethical issues regarding the use of embryos initially generated for reproductive purposes. The aim of this study was to obtain an optimized oocyte activation method through parthenogenesis by using mice oocytes as a model. Ten mM SrCl2 and 5 µg/ml Cytochalasin B (CB) in calcium-free Chatot Ziomek Bavister (CZB) were used as a medium for an in vitro activation of mouse oocytes. Treatment combinations for the oocyte activation methods were (A) activation in CZB & SrCl2 (prepared in stock) for two hours and in CZB & CB for four hours; (B) activation in CZB & SrCl2 (fresh medium) for two hours and in CZB & CB for four hours; and (C) activation in CZB & CB & SrCl2 (fresh medium) for six hours. The results show that the activation rate of  mouse oocytes  with  method C has  been  the best among all the protocols. This optimized protocol clearly provides a new insight in the generation of embryos for further use, particularly for producing embryonic stem cells.

Perkembangan Praimplantasi Embrio Mencit dengan Materi Genetik yang Berasal dari Parental, Maternal, dan Inti Sel Somatik (PRE-IMPLANTATION DEVELOPMENT OF MOUSE EMBRYO WITH GENETIC MATERIAL DERIVED FROM PARENTAL, MATERNAL AND SOMATIC CELL NUCLEUS)

Jurnal Veteriner Vol 15, No 1 (2014)
Publisher : Jurnal Veteriner

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Abstract

Cloned embryo and parthenogenetic embryo are a potential source of stem cells for regenerativemedicine. Stem cells derived from those embryos are expected to overcome the ethical issues to the use offertilization embryos for therapeutic purposes. The pre-implantation development is a critical step fordeveloping embryos reach the blastocyst stage. The objectives in vivo of this research are to produce mousecloned embryo, parthenogenetic embryo, and fertilized embryo and to study stages of  in vitro pre-implantation development culture. In vivo fertilized embryos, mouse oocytes, and cumulus cells were usedin this study. Treatment was performed on female mice superovulated with PMSG and hCG injections.Two-cell stage of in vivo fertilized embryos were collected on the second day post hCG injection. Clonedembryos were produced through Somatic Cell Nuclear Transfer (SCNT), which included enucleation, nucleartransfer and artificial activation. Parthenogenetic embryos were produced with artificial activationtechnique. The result of the research indicated that SCNT application was able to produce cloned embryos which could develop to blastocyst stage (3,2%). In addition, artificial activation of oocytes could produceparthenogenetic embryos which were able to develop up to the blastocyst stage (8,6%). In conclusion,efficiency level of parthenogenetic embryos that is able to reach the blastocyst stage was higher than in thecloned embryos. Fertilized embryos shows a better development and more efficient compared to in vitrocloned embryos and parthenogenetic embryos cultures.

PERKEMBANGAN EMBRIO SAPI SETELAH FERTILISASI MENGGUNAKAN METODE INTRACYTOPLASMIC SPERM INJECTION (ICSI) DAN AKTIVASI DENGAN STRONTIUM

Jurnal Kedokteran Hewan Vol 8, No 2 (2014): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

Penelitian ini bertujuan mengetahui pengaruh kombinasi intracytoplasmic sperm injection (ICSI) dan aktivasi dengan strontium untuk mengetahui perkembangan pronukleus dan perkembangan embrio sampai tahap blastosis. Kombinasi ICSI dan strontium 20 mM meningkatkan perkembangan pronukleus 2-PN mencapai 43,59%. Hasil perkembangan embrio pada perlakuan kombinasi ICSI dan strontium 20 mM mencapai tingkat perkembangan 2-4 sel (50,5%), 8-16 sel (43,73%), dan blastosis (15,63%). Kesimpulan dari hasil penelitian ini adalah kombinasi ICSI dengan aktivasi strontium 20 mM mampu menghasilkan perkembangan in vitro embrio sapi yang lebih baik.

Allotransplantasi Testis Mencit Muda sebagai Upaya Preservasi Gonad In Vivo

Jurnal Ilmu Pertanian Indonesia Vol 12, No 1 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Original Source | Check in Google Scholar | Full PDF (166.442 KB)

Abstract

Cancer disease are not detected only at adult but also at young age. One therapy for cancer diseases is chemotherapy and radiation, that give a side effect of infertility in the gonad, therefore, it is necessary to preserve the gonad. Sperm collection from adult is easy but not from the young patients.Keyword: transpalantation, testis, mencit muda, spermatogenesis

Relationship between Nucleus Swelling and Development Competence of Bovine Cloned Embryos Reconstructed by Enucleated Oocytes with Serum-starved or Serum-fed Fetal Somatic Cells

Indonesian Journal of Biotechnology Vol 10, No 2 (2005)
Publisher : Universitas Gadjah Mada

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Abstract

This study was conducted to examine the occurrence of nuclear remodeling (nucleus swelling) and its effectson the subsequent in vitro development of bovine embryos reconstructed by serum-starved and serum-fed somaticcells. Results from this study demonstrated that all of the reconstructed embryos that received serum-starved andserum-fed somatic cells exhibited condensed-nuclei. More than 90% of the transferred nuclei exhibited nuclearenvelope breakdown and premature chromatin condensation which clearly distinct from an intact nucleus. Therewas no significant difference on the degree of nucleus swelling in SS-NT embryos or SF-NT embryos, indicatingthat either serum-starved or confluent somatic cell lines could be reprogrammed by the recipient cytoplasmenvironments in similar pattern. Although the fusion rate was not significantly different among the groups, theproportion of SS-NT embryos which developed to the 2- to 4-cell stage (89.7%) and to the 8- to 16-cell stage (74.7%)was significantly higher than that of SF-NT embryos. Whereas, the proportion of reconstructed embryos thatdeveloped to the morula and blastocyst stages were not significantly different among the groups. Results of thesestudies demonstrate that reconstructed embryos, which received either serum-starved or serum-fed confluentsomatic cells, showed similar developmental competence to the blastocyst stage.Keywords: nuclear transplantation technique-somatic cells-nucleus swelling